Real-Time Polymerase Chain Reaction Assay on Bronchoalveolar Lavage: An Alternative Method for Diagnosing Chronic Pulmonary Schistosomiasis?

Federico Gobbi Center for Tropical Diseases (CTD), Sacro Cuore Don Calabria Hospital, Negrar, Verona, Italy;

Search for other papers by Federico Gobbi in
Current site
Google Scholar
PubMed
Close
,
Fabio Formenti Center for Tropical Diseases (CTD), Sacro Cuore Don Calabria Hospital, Negrar, Verona, Italy;

Search for other papers by Fabio Formenti in
Current site
Google Scholar
PubMed
Close
,
Francesca Perandin Center for Tropical Diseases (CTD), Sacro Cuore Don Calabria Hospital, Negrar, Verona, Italy;

Search for other papers by Francesca Perandin in
Current site
Google Scholar
PubMed
Close
,
Dora Buonfrate Center for Tropical Diseases (CTD), Sacro Cuore Don Calabria Hospital, Negrar, Verona, Italy;

Search for other papers by Dora Buonfrate in
Current site
Google Scholar
PubMed
Close
,
Andrea Angheben Center for Tropical Diseases (CTD), Sacro Cuore Don Calabria Hospital, Negrar, Verona, Italy;

Search for other papers by Andrea Angheben in
Current site
Google Scholar
PubMed
Close
,
Simona Paiano Pneumology Unit, Sacro Cuore Don Calabria Hospital, Negrar, Verona, Italy

Search for other papers by Simona Paiano in
Current site
Google Scholar
PubMed
Close
, and
Zeno Bisoffi Center for Tropical Diseases (CTD), Sacro Cuore Don Calabria Hospital, Negrar, Verona, Italy;

Search for other papers by Zeno Bisoffi in
Current site
Google Scholar
PubMed
Close
Restricted access

Schistosomiasis causes mainly hepatic and genitourinary damage. Although lung nodules have been commonly described in acute phase, they are presumably underdiagnosed in chronic schistosomiasis. We previously reported a series of patients with chronic pulmonary schistosomiasis confirmed by the histological examination of the lung biopsies. In the present work, we retrospectively tested an in-house real-time polymerase chain reaction for Schistosoma (currently validated for diagnosis on stool and on urine) in the bronchoalveolar lavage (BAL) of a couple of those patients, and both resulted positive. The possibility of testing BAL with molecular methods targeting a wide spectrum of pathogens, including parasites, is appealing. Further studies are needed to validate this technique that might reduce unnecessary biopsies.

Author Notes

Address correspondence to Federico Gobbi, Center for Tropical Diseases (CTD), Sacro Cuore Don Calabria Hospital, Via Sempreboni 5, 37024 Negrar, Verona, Italy. E-mail: federico.gobbi@sacrocuore.it

Authors’ addresses: Federico Gobbi, Fabio Formenti, Francesca Perandin, Dora Buonfrate, Andrea Angheben, and Zeno Bisoffi, Sacro Cuore Don Calabria Hospital, Centre for Tropical Diseases, Negrar, Verona, Italy, E-mails: federico.gobbi@sacrocuore.it, fabio.formenti@sacrocuore.it, francesca.perandin@sacrocuore.it, dora.buonfrate@sacrocuore.it, andrea.angheben@sacrocuore.it, and zeno.bisoffi@sacrocuore.it. Simona Paiano, Sacro Cuore Don Calabria Hospital, Pneumology Unit, Negrar, Verona, Italy, E-mail: simona.paiano@sacrocuore.it.

  • 1.

    Colley DG, Bustinduy AL, Secor WE, King CH, 2014. Human schistosomiasis. Lancet 383: 22532264.

  • 2.

    Niemann T, Marti HP, Duhnsen SH, Bongartz G, 2010. Pulmonary schistosomiasis–imaging features. J Radiol Case Rep 4: 3743.

  • 3.

    Ross AG, Vickers D, Olds GR, Shah SM, McManus DP, 2007. Katayama syndrome. Lancet Infect Dis 7: 218224.

  • 4.

    Papamatheakis DG, Mocumbi AO, Kim NH, Mandel J, 2014. Schistosomiasis associated pulmonary hypertension. Pulm Circ 4: 596611.

  • 5.

    Schwartz E, 2002. Pulmonary schistosomiasis. Clin Chest Med 23: 433443.

  • 6.

    Gobbi F et al. 2017. Pulmonary nodules in African migrants caused by chronic schistosomiasis. Lancet Infect Dis 17: e159e165.

  • 7.

    Obeng BB et al. 2008. Application of a circulating-cathodic-antigen (CCA) strip test and real-time PCR, in comparison with microscopy, for the detection of Schistosoma haematobium in urine samples from Ghana. Ann Trop Med Parasitol 102: 625633.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 8.

    Castelli F, Sulis G, 2017. Migration and infectious diseases. Clin Microbiol Infect 23: 283289.

  • 9.

    Kunst H, Mack D, Kon OM, Banerjee AK, Chiodini P, Grant A, 2011. Parasitic infections of the lung: a guide for the respiratory physician. Thorax 66: 528536.

  • 10.

    Utzinger J, Becker SL, van Lieshout L, van Dam GJ, Knopp S, 2015. New diagnostic tools in schistosomiasis. Clin Microbiol Infect 21: 529542.

Past two years Past Year Past 30 Days
Abstract Views 42 41 1
Full Text Views 327 8 0
PDF Downloads 56 0 0
 
 
 
 
Affiliate Membership Banner
 
 
Research for Health Information Banner
 
 
CLOCKSS
 
 
 
Society Publishers Coalition Banner
Save