ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
World Health Organization, 2016. The World Malaria Report 2016. Geneva, Switzerland: World Health Organization.
-
2.
Singh B, Kim Sung L, Matusop A, Radhakrishnan A, Shamsul SS, Cox-Singh J, Thomas A, Conway DJ, 2004. A large focus of naturally acquired Plasmodium knowlesi infections in human beings. Lancet 363: 1017–1024.
-
3.
Lee KS, Cox-Singh J, Singh B, 2009. Morphological features and differential counts of Plasmodium knowlesi parasites in naturally acquired human infections. Malar J 8: 73.
-
4.
Imwong M, Tanomsing N, Pukrittayakamee S, Day NP, White NJ, Snounou G, 2009. Spurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers currently used to detect P. knowlesi. J Clin Microbiol 47: 4173–4175.
-
5.
Lau YL, Lai MY, Anthony CA, Chang PY, Palaeya V, Fong MY, Mahmud R, 2015. Comparison of three molecular methods for the detection and speciation of five human Plasmodium species. Am J Trop Med Hyg 92: 28–33.
-
6.
Piepenburg O, Williams CH, Stemple DL, Armes NA, 2006. DNA detection using recombination protein. PLoS Biol 4: e204.
-
7.
Teoh BT, et al. 2015. Early detection of dengue virus by use of reverse transcription-recombinase polymerase amplification. J Clin Microbiol 53: 830–837.
-
8.
Abd El Wahed A, et al. 2015. Recombinase polymerase amplification assay for rapid diagnostics of dengue infection. PLoS One 10: e0129682.
-
9.
Kersting S, Rausch V, Bier FF, Nickisch-Rosenegk MV, 2014. Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis. Malar J 13: 99.
-
10.
Moorea MD, Jaykus LA, 2017. Development of a recombinase polymerase amplification assay for detection of epidemic human noroviruses. Sci Rep 7: 40244.
-
11.
Lau YL, Fong MY, Mahmud R, Chang PY, Palaeya V, Cheong FW, Chin LC, Anthony CN, Al-Mekhlafi AM, Chen Y, 2014. Specific, sensitive and rapid detection of human Plasmodium knowlesi infection by loop-mediated isothermal amplification (LAMP) in blood samples. Malar J 10: 197.
-
12.
Lau YL, Lai MY, Fong MY, Jelip J, Mahmud R, 2016. Comparison of three molecular methods for the detection and speciation of five human Plasmodium species. Am J Trop Med Hyg 94: 336–339.
-
13.
Yan L, Zhou J, Zheng Y, Gamson AS, Roembke BT, Nakayama S, Sintim HO, 2014. Isothermal amplified detection of DNA and RNA. Mol Biosyst 10: 970–1003.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 3 | 3 | 3 |
| Full Text Views | 545 | 265 | 3 |
| PDF Downloads | 165 | 39 | 3 |
Rapid Detection of Plasmodium knowlesi by Isothermal Recombinase Polymerase Amplification Assay
In this study, we developed a recombinase polymerase amplification (RPA) assay for specific diagnosis of Plasmodium knowlesi. Genomic DNA was extracted from whole blood samples using a commercial kit. With incubation at 37°C, the samples were successfully amplified within 20 minutes. The end product of RPA was further examined by loading onto agarose gel and a specific band was observed with a size of 128 bp. The RPA assay exhibited high sensitivity with limits of detection down to one copy of the plasmid. From the specificity experiments, it was demonstrated that all P. knowlesi samples (N = 45) were positive while other Plasmodium spp. (N = 42) and negative samples (N = 6) were negative. Therefore, the RPA assay is a highly promising approach with the potential to be used in resource-limited settings. This assay can be further optimized for bedside and on field application.
Author Notes
Financial support: This study was supported by University Malaya PPP Research Grant (PG054-2016A).
Authors’ addresses: Meng-Yee Lai and Yee-Ling Lau, Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia, E-mails: mengylai11@yahoo.com and lauyeeling@um.edu.my. Choo-Huck Ooi, Sarawak State Health Department, Jalan Diplomatik, Off Jalan Bako, Kuching, Sarawak, Malaysia, E-mail: ooi.choo.huck@gmail.com.