• 1.

    Meltzer E, Leshem E, Lustig Y, Gottesman G, Schwartz E, 2016. The clinical spectrum of Zika virus in returning travelers. Am J Med 129: 11261130.

  • 2.

    World Health Organization, 2016. WHO Director-General Summarizes the Outcome of the Emergency Committee Regarding Clusters of Microcephaly and Guillain-Barré Syndrome. Geneva, Switzerland: World Health Organization. Available at: http://www.who.int/mediacentre/news/statements/2016/emergency-committee-zika-microcephaly/en/. Accessed November 8, 2016.

  • 3.

    Campos GS, Bandeira AC, Sardi SI, 2015. Zika virus outbreak, Bahia, Brazil. Emerg Infect Dis 21: 18851886.

  • 4.

    Lanciotti RS, Kosoy OL, Laven JJ, Velez JO, Lambert AJ, Johnson AJ, Stanfield SM, Duffy MR, 2008. Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007. Emerg Infect Dis 14: 12321239.

    • Search Google Scholar
    • Export Citation
  • 5.

    Faye O, Faye O, Diallo D, Diallo M, Weidmann M, Sall A, 2013. Quantitative real-time PCR detection of Zika virus and evaluation with field-caught Mosquitoes. Virol J 10: 311.

    • Search Google Scholar
    • Export Citation
  • 6.

    L’Huillier AG, Lombos E, Tang E, Perusini S, Eshaghi A, Nagra S, Frantz C, Olsha R, Kristjanson E, Dimitrova K, Safronetz D, Drebot M, Gubbay JB, 2017. Evaluation of altona Diagnostics RealStar Zika Virus RT-PCR Test Kit for Zika virus PCR testing. J Clin Microbiol 55: 15761584.

    • Search Google Scholar
    • Export Citation
  • 7.

    Pessôa R, Patriota JV, de Souza M de L, Abd El Wahed A, Sanabani SS, 2016. Detection of Zika virus in Brazilian patients during the first five days of infection: urine versus plasma. Euro Surveill 21(30). Available at: http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=22542. Accessed July 28, 2016.

    • Search Google Scholar
    • Export Citation
  • 8.

    World Health Organization, 2016. Laboratory Testing for Zika Virus Infection Interim Guidance. WHO/ZIKV/LAB/161. Mar 23;4.

  • 9.

    Centers for Disease Control and Prevention, 2014. Zika Virus Diagnostic Tests Available at: http://www.cdc.gov/zika/hc-providers/types-of-tests.html. Accessed November 8, 2016.

 
 
 

 

 
 
 

 

 

 

 

 

 

Diagnostic Validation of the RealStar® Zika Virus Reverse Transcription Polymerase Chain Reaction Kit for Detection of Zika Virus RNA in Urine and Serum Specimens

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  • 1 altona Diagnostics GmbH, Hamburg, Germany;
  • | 2 Laboratoire de Virologie, CNR des Arbovirus, Institut Pasteur de la Guyane, Cayenne, French Guiana

With the Zika virus outbreak in South America starting in 2015 and its potential to cause malformation of the fetus in infected women, the need for diagnostic methods became obvious. Until now, only limited data are available on the diagnostic performance of commercial kits. Here, we present data comparing the RealStar® Zika Virus RT-PCR Kit 1.0 for detection of Zika virus from 208 serum and urine samples collected in French Guiana with a reference method. Of these, 114 samples tested positive with the RealStar® Kit and 111 with the reference method.

Author Notes

Address for correspondence to Stephan Ölschläger, altona Diagnostics GmbH, Mörkenstrasse 12, 22767 Hamburg, Germany. E-mail: stephan.oelschlaeger@gmail.com

Authors’ addresses: Stephan Ölschläger and Mareen Zaruba, altona Diagnostics GmbH, Research and Development, Hamburg, Germany, E-mails: stephan.oelschlaeger@gmail.com and mareen.zaruba@altona-diagnostics.com. Antoine Enfissi, Mirdad Kazanji, and Dominique Rousset, Laboratoire de virology Institut Pasteur de la Guyane, Laboratoire de virology, Cayenne, French Guiana, E-mails: aenfissi@pasteuer-cayenne.fr, mkazanji@pasteur-cayenne.fr, and drousset@pasteur-cayenne.fr.

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