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-
1.
Gunther G, 2014. Multidrug-resistant and extensively drug-resistant tuberculosis: a review of current concepts and future challenges. Clin Med (Lond) 14: 279–285.
-
2.
Wlodarska M, Johnston JC, Gardy JL, Tang P, 2015. A microbiological revolution meets an ancient disease: improving the management of tuberculosis with genomics. Clin Microbiol Rev 28: 523–539.
-
3.
van Embden JD et al., 1993. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol 31: 406–409.
-
4.
Kanduma E, McHugh TD, Gillespie SH, 2003. Molecular methods for Mycobacterium tuberculosis strain typing: a users guide. J Appl Microbiol 94: 781–791.
-
5.
Molina-Moya B, Latorre I, Lacoma A, Prat C, Domínguez J, 2014. Recent advances in tuberculosis diagnosis: IGRAs and molecular biology. Curr Treat Opt Infect Dis 6: 377e91.
-
6.
Gomgnimbou MK, Hernandez-Neuta I, Panaiotov S, Bachiyska E, Palomino JC, Martin A, del Portillo P, Refregier G, Sola C, 2013. Tuberculosis-spoligo-rifampin-isoniazid typing: an all-in-one assay technique for surveillance and control of multidrug-resistant tuberculosis on Luminex devices. J Clin Microbiol 51: 3527–3534.
-
7.
Espasa M, Salvado M, Vicente E, Tudo G, Alcaide F, Coll P, Martin-Casabona N, Torra M, Fontanals D, Gonzalez-Martin J, 2012. Evaluation of the VersaTREK system compared to the Bactec MGIT 960 system for first-line drug susceptibility testing of Mycobacterium tuberculosis. J Clin Microbiol 50: 488–491.
-
8.
Kamerbeek J et al., 1997. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol 35: 907–914.
-
9.
Portugal I, Maia S, Moniz-Pereira J, 1999. Discrimination of multidrug-resistant Mycobacterium tuberculosis IS6110 fingerprint subclusters by rpoB gene mutation analysis. J Clin Microbiol 37: 3022–3024.
-
10.
Garcia-Sierra N, Lacoma A, Prat C, Haba L, Maldonado J, Ruiz-Manzano J, Gavin P, Samper S, Ausina V, Dominguez J, 2011. Pyrosequencing for rapid molecular detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis strains and clinical specimens. J Clin Microbiol 49: 3683–3686.
-
11.
Gomgnimbou MK, Abadia E, Zhang J, Refregier G, Panaiotov S, Bachiyska E, Sola C, 2012. “Spoligoriftyping,” a dual-priming-oligonucleotide-based direct-hybridization assay for tuberculosis control with a multianalyte microbead-based hybridization system. J Clin Microbiol 50: 3172–3179.
-
12.
Abadia E et al., 2010. Resolving lineage assignation on Mycobacterium tuberculosis clinical isolates classified by spoligotyping with a new high-throughput 3R SNPs based method. Infect Genet Evol 10: 1066–1074.
-
13.
Perez-Lago L, Lirola MM, Navarro Y, Herranz M, Ruiz-Serrano MJ, Bouza E, Garcia-de-Viedma D, 2015. Co-infection with drug-susceptible and reactivated latent multidrug-resistant Mycobacterium tuberculosis. Emerg Infect Dis 21: 2098–2100.
-
14.
Flores L, Jarlsberg LG, Kim EY, Osmond D, Grinsdale J, Kawamura M, Desmond E, Hopewell PC, Kato-Maeda M, 2010. Comparison of restriction fragment length polymorphism with the polymorphic guanine-cytosine-rich sequence and spoligotyping for differentiation of Mycobacterium tuberculosis isolates with five or fewer copies of IS6110. J Clin Microbiol 48: 575–578.
-
15.
Zhang J, Abadia E, Refregier G, Tafaj S, Boschiroli ML, Guillard B, Andremont A, Ruimy R, Sola C, 2010. Mycobacterium tuberculosis complex CRISPR genotyping: improving efficiency, throughput and discriminative power of ‘spoligotyping’ with new spacers and a microbead-based hybridization assay. J Med Microbiol 59: 285–294.
-
16.
de Freitas FA et al., 2014. Multidrug resistant Mycobacterium tuberculosis: a retrospective katG and rpoB mutation profile analysis in isolates from a reference center in Brazil. PLoS One 9: e104100.
-
17.
Yasmin M, Gomgnimbou MK, Siddiqui RT, Refregier G, Sola C, 2014. Multi-drug resistant Mycobacterium tuberculosis complex genetic diversity and clues on recent transmission in Punjab, Pakistan. Infect Genet Evol 27C: 6–14.
-
18.
Dantas NG et al., 2015. Genetic diversity and molecular epidemiology of multidrug-resistant Mycobacterium tuberculosis in Minas Gerais State, Brazil. BMC Infect Dis 15: 306.
-
19.
Dominguez J et al., 2016. Clinical implications of molecular drug resistance testing for Mycobacterium tuberculosis: a TBNET/RESIST-TB consensus statement. Int J Tuberc Lung Dis 20: 24–42.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 242 | 194 | 14 |
| Full Text Views | 439 | 6 | 0 |
| PDF Downloads | 129 | 8 | 0 |
Molecular Characterization of Mycobacterium tuberculosis Strains with TB-SPRINT
Barbara Molina-Moya
Barbara Molina-Moya
Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d’Investigació Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Spain;
CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain;
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CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain;
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Michel Kiréopori Gomgnimbou
Michel Kiréopori Gomgnimbou
Institut de Biologie Intégrative de la Cellule (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France;
Centre Muraz, Bobo-Dioulasso, Burkina Faso
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Centre Muraz, Bobo-Dioulasso, Burkina Faso
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Carmen Lafoz
Carmen Lafoz
CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain;
Instituto Aragonés de Ciencias de la Salud, Fundación Instituto de Investigación Sanitaria de Aragón, Hospital Universitario Miguel Servet, Zaragoza, Spain;
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Instituto Aragonés de Ciencias de la Salud, Fundación Instituto de Investigación Sanitaria de Aragón, Hospital Universitario Miguel Servet, Zaragoza, Spain;
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Alicia Lacoma
Alicia Lacoma
Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d’Investigació Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Spain;
CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain;
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CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain;
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Cristina Prat
Cristina Prat
Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d’Investigació Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Spain;
CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain;
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CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain;
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Guislaine Refrégier
Guislaine Refrégier
Institut de Biologie Intégrative de la Cellule (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France;
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Sofia Samper
Sofia Samper
CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain;
Instituto Aragonés de Ciencias de la Salud, Fundación Instituto de Investigación Sanitaria de Aragón, Hospital Universitario Miguel Servet, Zaragoza, Spain;
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Instituto Aragonés de Ciencias de la Salud, Fundación Instituto de Investigación Sanitaria de Aragón, Hospital Universitario Miguel Servet, Zaragoza, Spain;
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Jose Dominguez
Jose Dominguez
Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d’Investigació Germans Trias i Pujol, Universitat Autònoma de Barcelona, Badalona, Spain;
CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain;
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CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain;
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Christophe Sola
Christophe Sola
Institut de Biologie Intégrative de la Cellule (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France;
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We evaluated Tuberculosis-Spoligo-Rifampicin-Isoniazid Typing (TB-SPRINT), a microbead-based method for spoligotyping and detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis. For that, 67 M. tuberculosis complex strains were retrospectively selected. Membrane-based spoligotyping, restriction fragment length polymorphism, DNA sequencing/pyrosequencing of rpoB, katG, and inhA promoter, TB-SPRINT, and SNP typing were performed. Concordance between spoligotyping methods was 99.6% (2,785/2,795 spoligotype data points). For most of the discordant cases, the same lineage was assigned with both methods. Concordance between phenotypic drug susceptibility testing and TB-SPRINT for detecting rifampicin and isoniazid resistance was 98.4% (63/64) and 93.8% (60/64), respectively. Concordance between DNA sequencing/pyrosequencing and TB-SPRINT for detecting mutations in rpoB, katG, and inhA were 98.4% (60/61), 100% (64/64), and 96.9% (62/64), respectively. In conclusion, TB-SPRINT is a rapid and easy-to-perform assay for genotyping and detecting drug resistance in a single tube; therefore, it may be a useful tool to improve epidemiological surveillance.
Author Notes
Financial support: The research was supported by grants from the Instituto de Salud Carlos III (PI07/0551, PI13/0154, PI15/0317, and PI16/01912).
Authors’ addresses: Barbara Molina-Moya and Jose Dominguez, Institut d’Investigació Germans Trias i Pujol, Microbiology, Badalona, Spain and CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, Spain, E-mails: barbaramolina88@gmail.com and jadomb@gmail.com. Michel Kiréopori Gomgnimbou, Guislaine Refrégier, and Christophe Sola, Institut de Biologie Intégrative de la Cellule (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay. Gif-sur-Yvette, France, E-mails: gomikir@yahoo.fr, guislaine.refregier@u-psud.fr, and christophe.sola@i2bc.paris-saclay.fr. Carmen Lafoz and Sofia Samper, Instituto Aragonés de Ciencias de la Salud, Fundación Instituto de Investigación Sanitaria de Aragón, Hospital Universitario Miguel Servet, Zaragoza, Spain, E-mails: clafoz@unizar.es and samper@unizar.es. Alicia Lacoma and Cristina Prat, Hospital Universitari Germans Trias i Pujol, Institut d’Investigació Germans Trias i Pujol, Universitat Autònoma de Barcelona, CIBER Enfermedades Respiratorias (CIBERES), Servei de Microbiologia, Badalona, Spain, E-mails: alicia.lacoma@gmail.com and cprat.germanstrias@gencat.cat.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Gunther G, 2014. Multidrug-resistant and extensively drug-resistant tuberculosis: a review of current concepts and future challenges. Clin Med (Lond) 14: 279–285.
-
2.
Wlodarska M, Johnston JC, Gardy JL, Tang P, 2015. A microbiological revolution meets an ancient disease: improving the management of tuberculosis with genomics. Clin Microbiol Rev 28: 523–539.
-
3.
van Embden JD et al., 1993. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol 31: 406–409.
-
4.
Kanduma E, McHugh TD, Gillespie SH, 2003. Molecular methods for Mycobacterium tuberculosis strain typing: a users guide. J Appl Microbiol 94: 781–791.
-
5.
Molina-Moya B, Latorre I, Lacoma A, Prat C, Domínguez J, 2014. Recent advances in tuberculosis diagnosis: IGRAs and molecular biology. Curr Treat Opt Infect Dis 6: 377e91.
-
6.
Gomgnimbou MK, Hernandez-Neuta I, Panaiotov S, Bachiyska E, Palomino JC, Martin A, del Portillo P, Refregier G, Sola C, 2013. Tuberculosis-spoligo-rifampin-isoniazid typing: an all-in-one assay technique for surveillance and control of multidrug-resistant tuberculosis on Luminex devices. J Clin Microbiol 51: 3527–3534.
-
7.
Espasa M, Salvado M, Vicente E, Tudo G, Alcaide F, Coll P, Martin-Casabona N, Torra M, Fontanals D, Gonzalez-Martin J, 2012. Evaluation of the VersaTREK system compared to the Bactec MGIT 960 system for first-line drug susceptibility testing of Mycobacterium tuberculosis. J Clin Microbiol 50: 488–491.
-
8.
Kamerbeek J et al., 1997. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol 35: 907–914.
-
9.
Portugal I, Maia S, Moniz-Pereira J, 1999. Discrimination of multidrug-resistant Mycobacterium tuberculosis IS6110 fingerprint subclusters by rpoB gene mutation analysis. J Clin Microbiol 37: 3022–3024.
-
10.
Garcia-Sierra N, Lacoma A, Prat C, Haba L, Maldonado J, Ruiz-Manzano J, Gavin P, Samper S, Ausina V, Dominguez J, 2011. Pyrosequencing for rapid molecular detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis strains and clinical specimens. J Clin Microbiol 49: 3683–3686.
-
11.
Gomgnimbou MK, Abadia E, Zhang J, Refregier G, Panaiotov S, Bachiyska E, Sola C, 2012. “Spoligoriftyping,” a dual-priming-oligonucleotide-based direct-hybridization assay for tuberculosis control with a multianalyte microbead-based hybridization system. J Clin Microbiol 50: 3172–3179.
-
12.
Abadia E et al., 2010. Resolving lineage assignation on Mycobacterium tuberculosis clinical isolates classified by spoligotyping with a new high-throughput 3R SNPs based method. Infect Genet Evol 10: 1066–1074.
-
13.
Perez-Lago L, Lirola MM, Navarro Y, Herranz M, Ruiz-Serrano MJ, Bouza E, Garcia-de-Viedma D, 2015. Co-infection with drug-susceptible and reactivated latent multidrug-resistant Mycobacterium tuberculosis. Emerg Infect Dis 21: 2098–2100.
-
14.
Flores L, Jarlsberg LG, Kim EY, Osmond D, Grinsdale J, Kawamura M, Desmond E, Hopewell PC, Kato-Maeda M, 2010. Comparison of restriction fragment length polymorphism with the polymorphic guanine-cytosine-rich sequence and spoligotyping for differentiation of Mycobacterium tuberculosis isolates with five or fewer copies of IS6110. J Clin Microbiol 48: 575–578.
-
15.
Zhang J, Abadia E, Refregier G, Tafaj S, Boschiroli ML, Guillard B, Andremont A, Ruimy R, Sola C, 2010. Mycobacterium tuberculosis complex CRISPR genotyping: improving efficiency, throughput and discriminative power of ‘spoligotyping’ with new spacers and a microbead-based hybridization assay. J Med Microbiol 59: 285–294.
-
16.
de Freitas FA et al., 2014. Multidrug resistant Mycobacterium tuberculosis: a retrospective katG and rpoB mutation profile analysis in isolates from a reference center in Brazil. PLoS One 9: e104100.
-
17.
Yasmin M, Gomgnimbou MK, Siddiqui RT, Refregier G, Sola C, 2014. Multi-drug resistant Mycobacterium tuberculosis complex genetic diversity and clues on recent transmission in Punjab, Pakistan. Infect Genet Evol 27C: 6–14.
-
18.
Dantas NG et al., 2015. Genetic diversity and molecular epidemiology of multidrug-resistant Mycobacterium tuberculosis in Minas Gerais State, Brazil. BMC Infect Dis 15: 306.
-
19.
Dominguez J et al., 2016. Clinical implications of molecular drug resistance testing for Mycobacterium tuberculosis: a TBNET/RESIST-TB consensus statement. Int J Tuberc Lung Dis 20: 24–42.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 242 | 194 | 14 |
| Full Text Views | 439 | 6 | 0 |
| PDF Downloads | 129 | 8 | 0 |