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RefWorks
Reference Manager
BibTeX
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EndNote
-
1.
Powers AM, Brault AC, Tesh RB, Weaver SC, 2000. Re-emergence of chikungunya and O’nyong-nyong viruses: evidence for distinct geographical lineages and distant evolutionary relationships. J Gen Virol 81: 471–479.
-
2.
Rwaguma EB, Lutwama JJ, Sempala SD, Kiwanuka N, Kamugisha J, Okware S, Bagambisa G, Lanciotti R, Roehrig JT, Gubler DJ, 1997. Emergence of epidemic O’nyong-nyong fever in southwestern Uganda, after an absence of 35 years. Emerg Infect Dis 3: 77.
-
3.
Shore H, 1961. O’nyong-nyong fever: an epidemic disease in east Africa. Trans R Soc Trop Med Hyg 55: 361–373.
-
4.
Vanlandingham DL, Hong C, Klingler K, Tsetsarkin K, McElroy KL, Powers AM, Lehane MJ, Higgs S, 2005. Differential infectivities of O’nyong-nyong and chikungunya virus isolates in Anopheles gambiae and Aedes aegypti mosquitoes. Am J Trop Med Hyg 72: 616–621.
-
5.
Williams MC, Woodall JP, Corbet PS, Gillett JD, 1965. O’nyong-nyong fever: an epidemic virus disease in East Africa. 8. Virus isolations from Anopheles mosquitoes. Trans R Soc Trop Med Hyg 59: 300–306.
-
6.
Haddow AJ, Davies CW, Walker AJ, 1960. O’nyong-nyong fever: an epidemic virus disease in east Africa. Trans R Soc Trop Med Hyg 54: 517–522.
-
7.
Johnson BK, Gichogo A, Gitau G, Patel N, Ademba G, Kirui R, Highton RB, Smith DH, 1981. Recovery of O’nyong-nyong virus from Anopheles funestus in western Kenya. Trans R Soc Trop Med Hyg 75: 239–241.
-
8.
Williams MC, Woodall JP, 1961. O’nyong-nyong fever: an epidemic virus disease in east Africa. II. Isolation and some properties of the virus. Trans R Soc Trop Med Hyg 55: 135–141.
-
9.
Lanciotti RS, Ludwig ML, Rwaguma EB, Lutwama JJ, Kram TM, Karabatsos N, Cropp BC, Miller BR, 1998. Emergence of epidemic O’nyong-nyong fever in Uganda after a 35-year absence: genetic characterization of the virus. Virology 252: 258–268.
-
10.
LaBeaud AD, Banda T, Brichard J, Muchiri EM, Mungai PL, Mutuku FM, Borland E, Gildengorin G, Pfeil S, Teng CY, Long K, Heise M, Powers AM, Kitron U, King CH, 2015. High rates of O’nyong nyong and chikungunya virus transmission in coastal Kenya. PLoS Negl Trop Dis 9: e0003436.
-
11.
Williams MC, Woodall JP, Gillett JD, 1965. O’nyong-nyong fever: an epidemic virus disease in east Africa. Vii. Virus isolations from man and serological studies up to July 1961. Trans R Soc Trop Med Hyg 59: 186–197.
-
12.
Tappe D, Kapaun A, Emmerich P, Campos Rde M, Cadar D, Gunther S, Schmidt-Chanasit J, 2014. O’nyong-nyong virus infection imported to Europe from Kenya by a traveler. Emerg Infect Dis 20: 1766–1767.
-
13.
Smith DR, Lee JS, Jahrling J, Kulesh DA, Turell MJ, Groebner JL, O’Guinn ML, 2009. Development of field-based real-time reverse transcription-polymerase chain reaction assays for detection of chikungunya and O’nyong-nyong viruses in mosquitoes. Am J Trop Med Hyg 81: 679–684.
-
14.
Waggoner JJ, Abeynayake J, Balassiano I, Lefterova M, Sahoo MK, Liu Y, Vital-Brazil JM, Gresh L, Balmaseda A, Harris E, Banaei N, Pinsky BA, 2014. A multiplex nucleic acid amplification test for the diagnosis of dengue, malaria, and leptospirosis. J Clin Microbiol 52: 2011–2018.
-
15.
Waggoner JJ, Ballesteros G, Gresh L, Mohamed-Hadley A, Tellez Y, Sahoo MK, Abeynayake J, Balmaseda A, Harris E, Pinsky BA, 2016. Clinical evaluation of a single-reaction real-time RT-PCR for pan-dengue and chikungunya virus detection. J Clin Virol 78: 57–61.
-
16.
Burd EM, 2010. Validation of laboratory-developed molecular assays for infectious diseases. Clin Microbiol Rev 23: 550–576.
-
17.
Waggoner JJ, Gresh L, Mohamed-Hadley A, Ballesteros G, Davila MJ, Tellez Y, Sahoo MK, Balmaseda A, Harris E, Pinsky BA, 2016. Single-reaction multiplex reverse transcription PCR for detection of Zika, chikungunya, and dengue Viruses. Emerg Infect Dis 22: 1295–1297.
-
18.
LaBeaud AD, Sutherland LJ, Muiruri S, Muchiri EM, Gray LR, Zimmerman PA, Hise AG, King CH, 2011. Arbovirus prevalence in mosquitoes, Kenya. Emerg Infect Dis 17: 233–241.
-
19.
Kading RC, Borland EM, Cranfield M, Powers AM, 2013. Prevalence of antibodies to alphaviruses and flaviviruses in free-ranging game animals and nonhuman primates in the greater Congo basin. J Wildl Dis 49: 587–599.
-
20.
Corbet PS, Williams MC, Gillett JD, 1961. O’Nyong-nyong fever: an epidemic virus disease in east Africa. IV. Vector studies at epidemic sites. Trans R Soc Trop Med Hyg 55: 463–480.
-
21.
Lutwama JJ, Kayondo J, Savage HM, Burkot TR, Miller BR, 1999. Epidemic O’nyong-nyong fever in southcentral Uganda, 1996–1997: entomologic studies in Bbaale village, Rakai District. Am J Trop Med Hyg 61: 158–162.
-
22.
De Zulueta J, Woodall JP, Cullen JR, Williams MC, Kafuko GW, Gillett JD, 1962. An observation on the possible effect of O’nyong-nyong fever on malaria. Bull World Health Organ 26: 135–139.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 883 | 710 | 16 |
| Full Text Views | 501 | 18 | 0 |
| PDF Downloads | 200 | 19 | 0 |
Development of a Real-Time Reverse Transcription Polymerase Chain Reaction for O’nyong-nyong Virus and Evaluation with Clinical and Mosquito Specimens from Kenya
Jesse Waggoner
Jesse Waggoner
Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia;
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Claire Jane Heath
Claire Jane Heath
Division of Infectious Diseases, Department of Pediatrics, Stanford University School of Medicine, Stanford, California;
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Bryson Ndenga
Bryson Ndenga
Kenya Medical Research Institute, Kisumu, Kenya;
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Francis Mutuku
Francis Mutuku
Technical University of Mombasa, Mombasa, Kenya;
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Malaya K. Sahoo
Malaya K. Sahoo
Department of Pathology, Stanford University School of Medicine, Stanford, California;
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Alisha Mohamed-Hadley
Alisha Mohamed-Hadley
Department of Pathology, Stanford University School of Medicine, Stanford, California;
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John Vulule
John Vulule
Kenya Medical Research Institute, Kisumu, Kenya;
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Dunstan Mukoko
Dunstan Mukoko
Ministry of Health, Nairobi, Kenya;
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A. Desiree LaBeaud
A. Desiree LaBeaud
Division of Infectious Diseases, Department of Pediatrics, Stanford University School of Medicine, Stanford, California;
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Benjamin A. Pinsky
Benjamin A. Pinsky
Department of Pathology, Stanford University School of Medicine, Stanford, California;
Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California
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Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, California
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O’nyong-nyong virus (ONNV), an alphavirus closely related to chikungunya virus (CHIKV), has been the documented cause of two large outbreaks in east Africa; however, little is known about the contribution of ONNV to cases of acute febrile illness during interepidemic periods. An ONNV real-time reverse transcription polymerase chain reaction (rRT-PCR) was developed and evaluated using clinical and mosquito pool samples. The ONNV rRT-PCR linear range extended from 8.0 to 2.0 log10 copies/μL, and the lower limit of 95% detection was 22.4 copies/μL. No cases of ONNV infection were identified in serum from 385 Kenyan children who presented with an acute febrile illness. Additionally, ONNV was not detected in 120 mosquito pools collected in coastal and western Kenya. The ONNV rRT-PCR demonstrated good analytical sensitivity when performed in monoplex or as a component of an ONNV–CHIKV duplex assay. This assay should provide a useful diagnostic for the detection of ONNV in surveillance studies.
Author Notes
Financial support: Research was supported by National Institutes of Health (NIH) grants K08AI110528 (JJW) and R01AI102918 (ADL). Funds for assay development were also provided by a Robert E. Shope International Fellowship in Infectious Diseases, distributed by the American Society for Tropical Medicine and Hygiene.
Authors’ addresses: Jesse Waggoner, Division of Infectious Diseases, Department of Medicine, Emory University, Atlanta, GA, E-mail: jesse.j.waggoner@emory.edu. Claire Jane Heath, Division of Infectious Diseases, Department of Pediatrics, Stanford University School of Medicine, Palo Alto, CA, E-mail: claireejane@gmail.com. Bryson Ndenga, Kenya Medical Research Institute, Kisian Campus, Kisumu, Kenya, E-mail: bndenga@yahoo.com. Francis Mutuku, Environmental Studies, Emory University, Atlanta, GA, and CWRU/DVBNTD, Diani, Kenya, E-mail: fmutuku73@gmail.com. Malaya K. Sahoo and Alisha Mohamed-Hadley, Department of Pathology, Stanford University School of Medicine, Palo Alto, CA, E-mails: mksahoo@stanford.edu and alisha.mohamedhadley@gmail.com. John Vulule, Center for Global Health Research, Kenya Medical Research Institute, Biomedical Sciences, Kisumu, Kenya, E-mail: jvulule@gmail.com. Dunstan Mukoko, Vector Borne Disease Control Unit, Vector Borne Disease Control Unit, Nairobi, Kenya, E-mail: dunstan.mukoko29@gmail.com. A. Desiree LaBeaud, Division of Infectious Diseases, Department of Pediatrics, Stanford University School of Medicine, Stanford, CA, E-mail: dlabeaud@stanford.edu. Benjamin A. Pinsky, Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, E-mail: bpinsky@stanford.edu.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Powers AM, Brault AC, Tesh RB, Weaver SC, 2000. Re-emergence of chikungunya and O’nyong-nyong viruses: evidence for distinct geographical lineages and distant evolutionary relationships. J Gen Virol 81: 471–479.
-
2.
Rwaguma EB, Lutwama JJ, Sempala SD, Kiwanuka N, Kamugisha J, Okware S, Bagambisa G, Lanciotti R, Roehrig JT, Gubler DJ, 1997. Emergence of epidemic O’nyong-nyong fever in southwestern Uganda, after an absence of 35 years. Emerg Infect Dis 3: 77.
-
3.
Shore H, 1961. O’nyong-nyong fever: an epidemic disease in east Africa. Trans R Soc Trop Med Hyg 55: 361–373.
-
4.
Vanlandingham DL, Hong C, Klingler K, Tsetsarkin K, McElroy KL, Powers AM, Lehane MJ, Higgs S, 2005. Differential infectivities of O’nyong-nyong and chikungunya virus isolates in Anopheles gambiae and Aedes aegypti mosquitoes. Am J Trop Med Hyg 72: 616–621.
-
5.
Williams MC, Woodall JP, Corbet PS, Gillett JD, 1965. O’nyong-nyong fever: an epidemic virus disease in East Africa. 8. Virus isolations from Anopheles mosquitoes. Trans R Soc Trop Med Hyg 59: 300–306.
-
6.
Haddow AJ, Davies CW, Walker AJ, 1960. O’nyong-nyong fever: an epidemic virus disease in east Africa. Trans R Soc Trop Med Hyg 54: 517–522.
-
7.
Johnson BK, Gichogo A, Gitau G, Patel N, Ademba G, Kirui R, Highton RB, Smith DH, 1981. Recovery of O’nyong-nyong virus from Anopheles funestus in western Kenya. Trans R Soc Trop Med Hyg 75: 239–241.
-
8.
Williams MC, Woodall JP, 1961. O’nyong-nyong fever: an epidemic virus disease in east Africa. II. Isolation and some properties of the virus. Trans R Soc Trop Med Hyg 55: 135–141.
-
9.
Lanciotti RS, Ludwig ML, Rwaguma EB, Lutwama JJ, Kram TM, Karabatsos N, Cropp BC, Miller BR, 1998. Emergence of epidemic O’nyong-nyong fever in Uganda after a 35-year absence: genetic characterization of the virus. Virology 252: 258–268.
-
10.
LaBeaud AD, Banda T, Brichard J, Muchiri EM, Mungai PL, Mutuku FM, Borland E, Gildengorin G, Pfeil S, Teng CY, Long K, Heise M, Powers AM, Kitron U, King CH, 2015. High rates of O’nyong nyong and chikungunya virus transmission in coastal Kenya. PLoS Negl Trop Dis 9: e0003436.
-
11.
Williams MC, Woodall JP, Gillett JD, 1965. O’nyong-nyong fever: an epidemic virus disease in east Africa. Vii. Virus isolations from man and serological studies up to July 1961. Trans R Soc Trop Med Hyg 59: 186–197.
-
12.
Tappe D, Kapaun A, Emmerich P, Campos Rde M, Cadar D, Gunther S, Schmidt-Chanasit J, 2014. O’nyong-nyong virus infection imported to Europe from Kenya by a traveler. Emerg Infect Dis 20: 1766–1767.
-
13.
Smith DR, Lee JS, Jahrling J, Kulesh DA, Turell MJ, Groebner JL, O’Guinn ML, 2009. Development of field-based real-time reverse transcription-polymerase chain reaction assays for detection of chikungunya and O’nyong-nyong viruses in mosquitoes. Am J Trop Med Hyg 81: 679–684.
-
14.
Waggoner JJ, Abeynayake J, Balassiano I, Lefterova M, Sahoo MK, Liu Y, Vital-Brazil JM, Gresh L, Balmaseda A, Harris E, Banaei N, Pinsky BA, 2014. A multiplex nucleic acid amplification test for the diagnosis of dengue, malaria, and leptospirosis. J Clin Microbiol 52: 2011–2018.
-
15.
Waggoner JJ, Ballesteros G, Gresh L, Mohamed-Hadley A, Tellez Y, Sahoo MK, Abeynayake J, Balmaseda A, Harris E, Pinsky BA, 2016. Clinical evaluation of a single-reaction real-time RT-PCR for pan-dengue and chikungunya virus detection. J Clin Virol 78: 57–61.
-
16.
Burd EM, 2010. Validation of laboratory-developed molecular assays for infectious diseases. Clin Microbiol Rev 23: 550–576.
-
17.
Waggoner JJ, Gresh L, Mohamed-Hadley A, Ballesteros G, Davila MJ, Tellez Y, Sahoo MK, Balmaseda A, Harris E, Pinsky BA, 2016. Single-reaction multiplex reverse transcription PCR for detection of Zika, chikungunya, and dengue Viruses. Emerg Infect Dis 22: 1295–1297.
-
18.
LaBeaud AD, Sutherland LJ, Muiruri S, Muchiri EM, Gray LR, Zimmerman PA, Hise AG, King CH, 2011. Arbovirus prevalence in mosquitoes, Kenya. Emerg Infect Dis 17: 233–241.
-
19.
Kading RC, Borland EM, Cranfield M, Powers AM, 2013. Prevalence of antibodies to alphaviruses and flaviviruses in free-ranging game animals and nonhuman primates in the greater Congo basin. J Wildl Dis 49: 587–599.
-
20.
Corbet PS, Williams MC, Gillett JD, 1961. O’Nyong-nyong fever: an epidemic virus disease in east Africa. IV. Vector studies at epidemic sites. Trans R Soc Trop Med Hyg 55: 463–480.
-
21.
Lutwama JJ, Kayondo J, Savage HM, Burkot TR, Miller BR, 1999. Epidemic O’nyong-nyong fever in southcentral Uganda, 1996–1997: entomologic studies in Bbaale village, Rakai District. Am J Trop Med Hyg 61: 158–162.
-
22.
De Zulueta J, Woodall JP, Cullen JR, Williams MC, Kafuko GW, Gillett JD, 1962. An observation on the possible effect of O’nyong-nyong fever on malaria. Bull World Health Organ 26: 135–139.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 883 | 710 | 16 |
| Full Text Views | 501 | 18 | 0 |
| PDF Downloads | 200 | 19 | 0 |