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Genotypic Distribution of Rotavirus in Phnom Penh, Cambodia: An Association of G9 with More Severe Diseases

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  • 1 Department of Enteric Diseases, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand.
  • | 2 National Pediatric Hospital, Phnom Penh, Cambodia.

Rotavirus causes significant morbidity and mortality among children worldwide. Stool samples from a previous hospital-based surveillance study to detect diarrhea etiology at the National Pediatric Hospital in Phnom Penh, Cambodia, by Meng and others in 2011 were tested for rotavirus by real-time reverse transcription polymerase chain reaction (PCR) targeting vp6 gene and characterized for G- and P-genotypes of positive samples based on vp7 and vp4 genes, respectively. Rotavirus was detected in 159/531 (30%) of children with diarrhea and none was detected in 287 nondiarrhea controls. All but three of the rotavirus-positive cases were children under the age of 2. The most common genotypes characterized by PCR and sequencing were G1P[8] (69%), G9P[8] (11%), and G2P[4] (11%). Genotype G9 was detected at a relatively high percentage that is consistent with the global trend and found to be associated with hospitalization. Data on disease burden and genotypic distribution are required information for the planning of rotavirus vaccine implementation in Cambodia.

Author Notes

* Address correspondence to Ladaporn Bodhidatta, Department of Enteric Diseases, Armed Forces Research Institute of Medical Sciences (AFRIMS), 315/6 Rajvithi Road, Bangkok, 10400 Thailand. E-mail: LadapornB.fsn@afrims.org

Financial support: This project was supported by Armed Forces Health Surveillance Center-Global Emerging Infections Surveillance (AFHSC-GEIS).

Authors' addresses: Sasikorn Silapong, Pimmada Sakpaisal, Ladaporn Bodhidatta, Paphavee Lertsethtakarn, Orntipa Sethabutr, and Brett E. Swierczewski, Department of Enteric Diseases, Armed Forces Research Institute of Medical Sciences (AFRIMS) Bangkok, Thailand, E-mails: sasikorns@afrims.org, pimmadaJ@afrims.org, ladapornb@afrims.org, paphaveeL@afrims.org, aey.orntipa@gmail.com, and brett.swierczewski.mil@afrims.org. Ket Vansith and Chhour Y. Meng, National Pediatric Hospital, Phnom Penh, Cambodia, E-mails: vansith52@gmail.com and cymeng.nph@online.com. Carl J. Mason, Seattle, WA, E-mail: carlmason@icloud.com.

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