Author:
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-
1.
Schär F, Trostdorf U, Giardina F, Khieu V, Muth S, Marti H, Vounatsou P, Odermatt P, 2013. Strongyloides stercoralis: global distribution and risk factors. PLoS Negl Trop Dis 7: e2288.
-
2.
Buonfrate D, Mena MA, Angheben A, Requena-Mendez A, Muñoz J, Gobbi F, Albonico M, Gotuzzo E, Bisoffi Z; COHEMI Project Study Group, 2015. Prevalence of strongyloidiasis in Latin America: a systematic review of the literature. Epidemiol Infect 143: 452–460.
-
3.
Bisoffi Z, Buonfrate D, Montresor A, Requena-Méndez A, Muñoz J, Krolewiecki AJ, Gotuzzo E, Mena MA, Chiodini PL, Anselmi M, Moreira J, Albonico M, 2013. Strongyloides stercoralis: a plea for action. PLoS Negl Trop Dis 7: e2214.
-
4.
Requena-Méndez A, Chiodini P, Bisoffi Z, Buonfrate D, Gotuzzo E, Muñoz J, 2013. The laboratory diagnosis and follow up of strongyloidiasis: a systematic review. PLoS Negl Trop Dis 7: e2002.
-
5.
Hernández-Chavarría F, Avendaño L, 2001. A simple modification of the Baermann method for diagnosis of strongyloidiasis. Mem Inst Oswaldo Cruz 96: 805–807.
-
6.
Arakaki T, Iwanaga M, Kinjo F, Saito A, Asato R, Ikeshiro T, 1990. Efficacy of agar-plate culture in detection of Strongyloides stercoralis infection. J Parasitol 76: 425–428.
-
7.
Koga K, Kasuya S, Khamboonruang C, Sukhavat K, Ieda M, Takatsuka N, Kita K, Ohtomo H, 1991. A modified agar plate method for detection of Strongyloides stercoralis. Am J Trop Med Hyg 45: 518–521.
-
8.
Uparanukraw P, Phongsri S, Morakote N, 1999. Fluctuations of larval excretion in Strongyloides stercoralis infection. Am J Trop Med Hyg 60: 967–973.
-
9.
Ribero Z, Chourio G, Díaz I, Padilla L, Zárate M, Ferrer J, 2002. Comparación de cuatro técnicas de laboratorio para el diagnóstico de estrongiloidiasis. Rev Soc Ven Microbiol 22: 68–73.
-
10.
Salazar SA, Gutierrez C, Berk SL, 1995. Value of the agar plate method for the diagnosis of intestinal strongyloidiasis. Diagn Microbiol Infect Dis 23: 141–145.
-
11.
Campo-Polanco L, Gutiérrez LA, Cardona-Arias J, 2014. Infección por Strongyloides stercoralis: metanálisis sobre evaluación de métodos diagnósticos convencionales (1980–2013). Rev Esp Salud Publica 88: 581–600.
-
12.
Ahmed NH, 2014. Cultivation of parasites. Trop Parasitol 4: 80–89.
-
13.
Khieu V, Schär F, Marti H, Sayasone S, Duong S, Muth S, Odermatt P, 2013. Diagnosis, treatment and risk factors of Strongyloides stercoralis in schoolchildren in Cambodia. PLoS Negl Trop Dis 7: e2035.
-
14.
Steinmann P, Zhou XN, Du ZW, Jiang JY, Wang LB, Wang XZ, Li LH, Marti H, Utzinger J, 2007. Occurrence of Strongyloides stercoralis in Yunnan Province, China, and comparison of diagnostic methods. PLoS Negl Trop Dis 1: e75.
-
15.
Becker SL, Sieto B, Silué KD, Adjossan L, Koné S, Hatz C, Kern WV, N'Goran EK, Utzinger J, 2011. Diagnosis, clinical features, and self-reported morbidity of Strongyloides stercoralis and hookworm infection in a co-endemic setting. PLoS Negl Trop Dis 5: e1292.
-
16.
Siddiqui AA, Berk SL, 2001. Diagnosis of Strongyloides stercoralis infection. Clin Infect Dis 33: 1040–1047.
-
17.
Pilotte N, Papaiakovou M, Grant JR, Bierwert LA, Llewellyn S, McCarthy JS, Williams S, 2016. Improved PCR-based detection of soil transmitted helminth infections using a next-generation sequencing approach to assay design. PLoS Negl Trop Dis 10: e0004578.
-
18.
Llewellyn S, Inpankaew T, Nery SV, Gray DJ, Verweij JJ, Clements ACA, Gomes SJ, Traub R, McCarthy JS, 2016. Application of a multiplex quantitative PCR to assess prevalence and intensity of intestinal parasite infections in a controlled clinical trial. PLoS Negl Trop Dis 10: e0004380.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 41 | 41 | 14 |
| Full Text Views | 572 | 151 | 1 |
| PDF Downloads | 297 | 37 | 0 |
Improved Detection of Strongyloides stercoralis in Modified Agar Plate Cultures
Leonor A. Pocaterra
Leonor A. Pocaterra
Cátedra de Parasitología, Escuela de Medicina “José María Vargas,” Universidad Central de Venezuela, Caracas, Venezuela.
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Giuseppe Ferrara
Giuseppe Ferrara
Laboratorio de Parasitosis Intestinales, Escuela de Medicina “José María Vargas,” Universidad Central de Venezuela, Caracas, Venezuela.
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Rosaura Peñaranda
Rosaura Peñaranda
Cátedra de Parasitología, Escuela de Medicina “José María Vargas,” Universidad Central de Venezuela, Caracas, Venezuela.
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Elsy Rojas
Elsy Rojas
Laboratorio de Parasitosis Intestinales, Escuela de Medicina “José María Vargas,” Universidad Central de Venezuela, Caracas, Venezuela.
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Gladymar Pérez-Chacón
Gladymar Pérez-Chacón
Cátedra de Parasitología, Escuela de Medicina “José María Vargas,” Universidad Central de Venezuela, Caracas, Venezuela.
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Aurora Hernán
Aurora Hernán
Cátedra de Parasitología, Escuela de Medicina “José María Vargas,” Universidad Central de Venezuela, Caracas, Venezuela.
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Gabriela Certad
Gabriela Certad
Cátedra de Parasitología, Escuela de Medicina “José María Vargas,” Universidad Central de Venezuela, Caracas, Venezuela.
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Carlos Goldstein
Carlos Goldstein
Medicina Interna-Hematología, Centro Médico de Caracas, Caracas, Venezuela.
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Luz Núñez
Luz Núñez
Cátedra de Parasitología, Escuela de Medicina “José María Vargas,” Universidad Central de Venezuela, Caracas, Venezuela.
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A modification of Koga agar plate culture was performed, consisting of a 2 × 2-cm cellophane paper centered on the agar plate to prevent bacterial contamination of the agar and daily dish examinations (days 2–5). Between January 2000 and July 2005, we examined 1,708 infection-suspected patients, of which 147 (8.6%) harbored S. stercoralis. Single modified agar plate cultures exhibited superior sensitivity (93.2%), compared with different three-sample screening methods (sensitivity—Baermann: 76.6%, formalin-ethyl acetate: 22%, and direct smear: 15.3%). Agar plate cultures stand out as helpful alternatives for improved detection and therapy monitoring in poor countries and endemic areas. Combined with Baermann methods, they provide increased probability for S. stercoralis detection.
Author Notes
Authors' addresses: Leonor A. Pocaterra, Rosaura Peñaranda, Elsy Rojas, Gladymar Pérez-Chacón, Aurora Hernán, Gabriela Certad, and Luz Núñez, Cátedra de Parasitología, “Escuela de Medicina Jose Maria Vargas,” Universidad Central de Venezuela, Caracas, Venezuela, E-mails: leopoca@gmail.com, rosaura_penaranda@yahoo.com.br, elsyrjs@hotmail.com, gladymar@gmail.com, aurorahrnn@hotmail.com, gabrielacertad@hotmail.com, and nenagold@gmail.com. Giuseppe Ferrara, Laboratorio de Parasitosis Intestinales, Cátedra de Parasitología, Escuela de Medicina “José María Vargas,” Universidad Central de Venezuela, Caracas, Venezuela, E-mail: gferrara1971@gmail.com. Carlos Goldstein, Medicina Interna-Hematología, Centro Médico de Caracas, Caracas, Venezuela, E-mail: carlosgolds@gmail.com.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
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-
1.
Schär F, Trostdorf U, Giardina F, Khieu V, Muth S, Marti H, Vounatsou P, Odermatt P, 2013. Strongyloides stercoralis: global distribution and risk factors. PLoS Negl Trop Dis 7: e2288.
-
2.
Buonfrate D, Mena MA, Angheben A, Requena-Mendez A, Muñoz J, Gobbi F, Albonico M, Gotuzzo E, Bisoffi Z; COHEMI Project Study Group, 2015. Prevalence of strongyloidiasis in Latin America: a systematic review of the literature. Epidemiol Infect 143: 452–460.
-
3.
Bisoffi Z, Buonfrate D, Montresor A, Requena-Méndez A, Muñoz J, Krolewiecki AJ, Gotuzzo E, Mena MA, Chiodini PL, Anselmi M, Moreira J, Albonico M, 2013. Strongyloides stercoralis: a plea for action. PLoS Negl Trop Dis 7: e2214.
-
4.
Requena-Méndez A, Chiodini P, Bisoffi Z, Buonfrate D, Gotuzzo E, Muñoz J, 2013. The laboratory diagnosis and follow up of strongyloidiasis: a systematic review. PLoS Negl Trop Dis 7: e2002.
-
5.
Hernández-Chavarría F, Avendaño L, 2001. A simple modification of the Baermann method for diagnosis of strongyloidiasis. Mem Inst Oswaldo Cruz 96: 805–807.
-
6.
Arakaki T, Iwanaga M, Kinjo F, Saito A, Asato R, Ikeshiro T, 1990. Efficacy of agar-plate culture in detection of Strongyloides stercoralis infection. J Parasitol 76: 425–428.
-
7.
Koga K, Kasuya S, Khamboonruang C, Sukhavat K, Ieda M, Takatsuka N, Kita K, Ohtomo H, 1991. A modified agar plate method for detection of Strongyloides stercoralis. Am J Trop Med Hyg 45: 518–521.
-
8.
Uparanukraw P, Phongsri S, Morakote N, 1999. Fluctuations of larval excretion in Strongyloides stercoralis infection. Am J Trop Med Hyg 60: 967–973.
-
9.
Ribero Z, Chourio G, Díaz I, Padilla L, Zárate M, Ferrer J, 2002. Comparación de cuatro técnicas de laboratorio para el diagnóstico de estrongiloidiasis. Rev Soc Ven Microbiol 22: 68–73.
-
10.
Salazar SA, Gutierrez C, Berk SL, 1995. Value of the agar plate method for the diagnosis of intestinal strongyloidiasis. Diagn Microbiol Infect Dis 23: 141–145.
-
11.
Campo-Polanco L, Gutiérrez LA, Cardona-Arias J, 2014. Infección por Strongyloides stercoralis: metanálisis sobre evaluación de métodos diagnósticos convencionales (1980–2013). Rev Esp Salud Publica 88: 581–600.
-
12.
Ahmed NH, 2014. Cultivation of parasites. Trop Parasitol 4: 80–89.
-
13.
Khieu V, Schär F, Marti H, Sayasone S, Duong S, Muth S, Odermatt P, 2013. Diagnosis, treatment and risk factors of Strongyloides stercoralis in schoolchildren in Cambodia. PLoS Negl Trop Dis 7: e2035.
-
14.
Steinmann P, Zhou XN, Du ZW, Jiang JY, Wang LB, Wang XZ, Li LH, Marti H, Utzinger J, 2007. Occurrence of Strongyloides stercoralis in Yunnan Province, China, and comparison of diagnostic methods. PLoS Negl Trop Dis 1: e75.
-
15.
Becker SL, Sieto B, Silué KD, Adjossan L, Koné S, Hatz C, Kern WV, N'Goran EK, Utzinger J, 2011. Diagnosis, clinical features, and self-reported morbidity of Strongyloides stercoralis and hookworm infection in a co-endemic setting. PLoS Negl Trop Dis 5: e1292.
-
16.
Siddiqui AA, Berk SL, 2001. Diagnosis of Strongyloides stercoralis infection. Clin Infect Dis 33: 1040–1047.
-
17.
Pilotte N, Papaiakovou M, Grant JR, Bierwert LA, Llewellyn S, McCarthy JS, Williams S, 2016. Improved PCR-based detection of soil transmitted helminth infections using a next-generation sequencing approach to assay design. PLoS Negl Trop Dis 10: e0004578.
-
18.
Llewellyn S, Inpankaew T, Nery SV, Gray DJ, Verweij JJ, Clements ACA, Gomes SJ, Traub R, McCarthy JS, 2016. Application of a multiplex quantitative PCR to assess prevalence and intensity of intestinal parasite infections in a controlled clinical trial. PLoS Negl Trop Dis 10: e0004380.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 41 | 41 | 14 |
| Full Text Views | 572 | 151 | 1 |
| PDF Downloads | 297 | 37 | 0 |