Author:
ProCite
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Reference Manager
BibTeX
Zotero
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-
1.
World Health Organization, 2013. World Malaria Report. Available at: http://www.who.int/iris/bitstream/10665/97008/1/9789241564694_eng.pdf.
-
2.
Cotter C, Sturrock HJW, Hsiang MS, Liu J, Phillips AA, Hwang J, Gueye CS, Fullman N, Gosling RD, Feachem RGA, 2013. The changing epidemiology of malaria elimination: new strategies for new challenges. Lancet 382: 7.
-
3.
Okell LC, Bousema T, Griffin JT, Ouedraogo AL, Ghani AC, Drakeley CJ, 2012. Factors determining the occurrence of submicroscopic malaria infections and their relevance for control. Nat Commun 3: 1237.
-
4.
Shekalaghe SA, Bousema JT, Kunei KK, Lushino P, Masokoto A, Wolters LR, Mwakalinga S, Mosha FW, Sauerwein RW, Drakeley CJ, 2007. Submicroscopic Plasmodium falciparum gametocyte carriage is common in an area of low and seasonal transmission in Tanzania. Trop Med Int Health 12: 547.
-
5.
Okell LC, Ghani AC, Lyons E, Drakeley CJ, 2009. Submicroscopic infection in Plasmodium falciparum endemic populations: a systematic review and meta-analysis. J Infect Dis 200: 1509–1517.
-
6.
Schneider P, Bousema JT, Gouagna LC, Otieno S, van de Vegte-Bolmer M, Omar SA, Sauerwein RW, 2007. Submicroscopic Plasmodium falciparum gametocyte densities frequently result in mosquito infection. Am J Trop Med Hyg 76: 470.
-
7.
Dal-Bianco MP, Köster KB, Kombila UD, Kun JFJ, Grobusch MP, Mombo-Ngoma G, Matsiegui PB, Supan C, Salazar CLO, Missinou MA, Issifou S, Lell B, Kremsner P, 2007. High prevalence of asymptomatic Plasmodium falciparum infection in Gabonese adults. Am J Trop Med Hyg 77: 939–942.
-
8.
Bouyou-Akotet MK, Mawili-Mboumba DP, Kendjo E, Mabika-Mamfoumbi M, Ngoungou EB, Dzeing-Ella A, Pemba-Mihindou M, Ibinga E, Efame-Eya E; MCRU Team, Planche T, Kremsner PG, Kombila M, 2009. Evidence of decline of malaria in the general hospital of Libreville, Gabon from 2000 to 2008. Malar J 8: 300.
-
9.
Mawili-Mboumba DP, Bouyou Akotet MK, Kendjo E, Nzamba J, Medang Owono M, Mourou Mbina JR, Kombila M; MCORU Team, 2013. Increase in malaria prevalence and age of at risk population in different areas of Gabon. Malar J 12: 3.
-
10.
Planche T, Krishna S, Kombila M, Engel K, Faucher JF, Ngou-Milama E, Kremsner PG, 2001. Comparison of methods for the rapid laboratory assessment of children with malaria. Am J Trop Med Hyg 65: 599–602.
-
11.
Mawili-Mboumba DP, Nikiéma R, Bouyou-Akotet MK, Bahamontes-Rosa N, Traoré A, Kombila M, 2013. Sub-microscopic gametocyte carriage in febrile children living in different areas of Gabon. Malar J 12: 375.
-
12.
Schneider P, Wolters L, Schoone G, Schallig H, Sillekens P, Hermsen R, Sauerwein R, 2005. Real-time nucleic acid sequence based amplification is more convenient than real-time PCR for quantification of Plasmodium falciparum. J Clin Microbiol 43: 402–405.
-
13.
Ouédraogo AL, Gonçalves BP, Gnémé A, Wenger EA, Guelbeogo MW, Ouédraogo A, Gerardin J, Bever CA, Lyons H, Pitroipa X, Verhave JP, Eckhoff PA, Drakeley C, Sauerwein R, Luty AJ, Kouyaté B, Bousema T, 2016. Dynamics of the human infectious reservoir for malaria determined by mosquito feeding assays and ultrasensitive malaria diagnosis in Burkina Faso. J Infect Dis 213: 90–99.
-
14.
Chen I, Clarke SE, Gosling R, Hamainza B, Killeen G, Magill A, O'Meara W, Price RN, Riley EM, 2016. “Asymptomatic” malaria: a chronic and debilitating infection that should be treated. PLoS Med 19: e1001942.
-
15.
Bouyou-Akotet MK, Tshibola ML, Mawili-Mboumba DP, Nzong J, Bahamontes-Rosa N, Tsoumbou-Bakana G, Kombila M, 2015. Frequencies of dhfr/dhps multiple mutations and Plasmodium falciparum submicroscopic gametocyte carriage in Gabonese pregnant women following IPTp-SP implementation. Acta Parasitol 60: 2.
-
16.
Mwingira F, Genton B, Kabanywanyi ANM, Felger I, 2014. Comparison of detection methods to estimate asexual Plasmodium falciparum parasite prevalence and gametocyte carriage in a community survey in Tanzania. Malar J 13: 433.
-
17.
Ouédraogo AL, Schneider P, de Kruijf M, Nébié I, Verhave JP, Cuzin-Ouattara N, Sauerwein RW, 2007. Age-dependent distribution of Plasmodium falciparum gametocytes quantified by Pfs25 real-time QT-NASBA in a cross-sectional study in Burkina Faso. Am J Trop Med Hyg 76: 626–630.
-
18.
Manjurano A, Okell L, Lukindo T, Reyburn H, Olomi R, Roper C, Clark TG, Joseph S, Riley EM, Drakeley C, 2011. Association of sub-microscopic malaria parasite carriage with transmission intensity in north-eastern Tanzania. Malar J 10: 370.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 53 | 52 | 12 |
| Full Text Views | 294 | 95 | 1 |
| PDF Downloads | 105 | 33 | 0 |
Submicroscopic Falciparum Malaria in Febrile Individuals in Urban and Rural Areas of Gabon
Denise P. Mawili-Mboumba
Denise P. Mawili-Mboumba
Department of Parasitology-Mycology, Faculty of Medicine, Université des Sciences de la Santé, Libreville, Gabon.
Malaria Clinical and Operational Research Unit, Libreville, Gabon.
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Malaria Clinical and Operational Research Unit, Libreville, Gabon.
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Rosalie Nikiéma Ndong
Rosalie Nikiéma Ndong
Department of Parasitology-Mycology, Faculty of Medicine, Université des Sciences de la Santé, Libreville, Gabon.
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Noemi Bahamontes Rosa
Noemi Bahamontes Rosa
Diseases of the Developing World, GlaxoSmithKline, Madrid, Spain.
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Jose Luis Llergo Largo
Jose Luis Llergo Largo
Diseases of the Developing World, GlaxoSmithKline, Madrid, Spain.
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Aude Lembet-Mikolo
Aude Lembet-Mikolo
Department of Parasitology-Mycology, Faculty of Medicine, Université des Sciences de la Santé, Libreville, Gabon.
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Prycil Nzamba
Prycil Nzamba
Department of Parasitology-Mycology, Faculty of Medicine, Université des Sciences de la Santé, Libreville, Gabon.
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Christelle Offouga Mbouoronde
Christelle Offouga Mbouoronde
Department of Parasitology-Mycology, Faculty of Medicine, Université des Sciences de la Santé, Libreville, Gabon.
Malaria Clinical and Operational Research Unit, Libreville, Gabon.
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Malaria Clinical and Operational Research Unit, Libreville, Gabon.
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Maryvonne Kombila
Maryvonne Kombila
Department of Parasitology-Mycology, Faculty of Medicine, Université des Sciences de la Santé, Libreville, Gabon.
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Marielle K. Bouyou Akotet
Marielle K. Bouyou Akotet
Department of Parasitology-Mycology, Faculty of Medicine, Université des Sciences de la Santé, Libreville, Gabon.
Malaria Clinical and Operational Research Unit, Libreville, Gabon.
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Characterization of the parasite reservoir is required to improve malaria control. Asymptomatic patients with subpatent parasitemia have been identified in Gabon, but the prevalence of such infections among febrile subjects is unclear. We assessed the prevalence of submicroscopic Plasmodium falciparum infections on an island (Port-Gentil), and in urban (Libreville), semiurban (Melen), and rural (Oyem) settings in Gabon. Blood samples (N = 310) from febrile patients were tested for malaria parasites by quantitative nucleic acid sequence–based amplification (QT-NASBA). Parasites were detected in 55.8% (173/310) of samples by microscopy and in 66.4% (206/310) of samples by 18S rRNA QT-NASBA. The proportion of submicroscopic infections differed considerably between sites. Gametocytes were found in 1% (3/310) of the individuals by microscopy and in 32% (99/310) by Pfs25 mRNA QT-NASBA. Thus, submicroscopic parasitemia is frequent in febrile patients, and the detection of this condition is important, to improve disease control.
Author Notes
Authors' addresses: Denise P. Mawili-Mboumba, Christelle Offouga Mbouoronde, and Marielle K. Bouyou Akotet, Department of Parasitology-Mycology, Faculty of Medicine, Université des Sciences de la Santé, Libreville, Gabon, and Malaria Clinical Research and Operational Unit, Centre Hospitalier de Libreville, Libreville, Gabon, E-mails: dpmawili@gmail.com, offougachristi@yahoo.fr, and mariellebouyou@gmail.com. Rosalie Nikiéma Ndong Ella, Aude Lembet-Mikolo, Prycil Nzamba, and Maryvonne Kombila, Department of Parasitology-Mycology, Faculty of Medicine, Université des Sciences de la Santé, Libreville, Gabon, E-mails: rosalienikiema@yahoo.fr, ttanguy01@yahoo.fr, kennygeordy@yahoo.fr, and valentine_favry@yahoo.fr. Noemi Bahamontes Rosa and Jose Luis Llergo Largo, Diseases of the Developing World, GlaxoSmithKline, Madrid, Spain, E-mails: noemi.r.bahamontes-rosa@gsk.com and jose.l.llergo@gsk.com.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
World Health Organization, 2013. World Malaria Report. Available at: http://www.who.int/iris/bitstream/10665/97008/1/9789241564694_eng.pdf.
-
2.
Cotter C, Sturrock HJW, Hsiang MS, Liu J, Phillips AA, Hwang J, Gueye CS, Fullman N, Gosling RD, Feachem RGA, 2013. The changing epidemiology of malaria elimination: new strategies for new challenges. Lancet 382: 7.
-
3.
Okell LC, Bousema T, Griffin JT, Ouedraogo AL, Ghani AC, Drakeley CJ, 2012. Factors determining the occurrence of submicroscopic malaria infections and their relevance for control. Nat Commun 3: 1237.
-
4.
Shekalaghe SA, Bousema JT, Kunei KK, Lushino P, Masokoto A, Wolters LR, Mwakalinga S, Mosha FW, Sauerwein RW, Drakeley CJ, 2007. Submicroscopic Plasmodium falciparum gametocyte carriage is common in an area of low and seasonal transmission in Tanzania. Trop Med Int Health 12: 547.
-
5.
Okell LC, Ghani AC, Lyons E, Drakeley CJ, 2009. Submicroscopic infection in Plasmodium falciparum endemic populations: a systematic review and meta-analysis. J Infect Dis 200: 1509–1517.
-
6.
Schneider P, Bousema JT, Gouagna LC, Otieno S, van de Vegte-Bolmer M, Omar SA, Sauerwein RW, 2007. Submicroscopic Plasmodium falciparum gametocyte densities frequently result in mosquito infection. Am J Trop Med Hyg 76: 470.
-
7.
Dal-Bianco MP, Köster KB, Kombila UD, Kun JFJ, Grobusch MP, Mombo-Ngoma G, Matsiegui PB, Supan C, Salazar CLO, Missinou MA, Issifou S, Lell B, Kremsner P, 2007. High prevalence of asymptomatic Plasmodium falciparum infection in Gabonese adults. Am J Trop Med Hyg 77: 939–942.
-
8.
Bouyou-Akotet MK, Mawili-Mboumba DP, Kendjo E, Mabika-Mamfoumbi M, Ngoungou EB, Dzeing-Ella A, Pemba-Mihindou M, Ibinga E, Efame-Eya E; MCRU Team, Planche T, Kremsner PG, Kombila M, 2009. Evidence of decline of malaria in the general hospital of Libreville, Gabon from 2000 to 2008. Malar J 8: 300.
-
9.
Mawili-Mboumba DP, Bouyou Akotet MK, Kendjo E, Nzamba J, Medang Owono M, Mourou Mbina JR, Kombila M; MCORU Team, 2013. Increase in malaria prevalence and age of at risk population in different areas of Gabon. Malar J 12: 3.
-
10.
Planche T, Krishna S, Kombila M, Engel K, Faucher JF, Ngou-Milama E, Kremsner PG, 2001. Comparison of methods for the rapid laboratory assessment of children with malaria. Am J Trop Med Hyg 65: 599–602.
-
11.
Mawili-Mboumba DP, Nikiéma R, Bouyou-Akotet MK, Bahamontes-Rosa N, Traoré A, Kombila M, 2013. Sub-microscopic gametocyte carriage in febrile children living in different areas of Gabon. Malar J 12: 375.
-
12.
Schneider P, Wolters L, Schoone G, Schallig H, Sillekens P, Hermsen R, Sauerwein R, 2005. Real-time nucleic acid sequence based amplification is more convenient than real-time PCR for quantification of Plasmodium falciparum. J Clin Microbiol 43: 402–405.
-
13.
Ouédraogo AL, Gonçalves BP, Gnémé A, Wenger EA, Guelbeogo MW, Ouédraogo A, Gerardin J, Bever CA, Lyons H, Pitroipa X, Verhave JP, Eckhoff PA, Drakeley C, Sauerwein R, Luty AJ, Kouyaté B, Bousema T, 2016. Dynamics of the human infectious reservoir for malaria determined by mosquito feeding assays and ultrasensitive malaria diagnosis in Burkina Faso. J Infect Dis 213: 90–99.
-
14.
Chen I, Clarke SE, Gosling R, Hamainza B, Killeen G, Magill A, O'Meara W, Price RN, Riley EM, 2016. “Asymptomatic” malaria: a chronic and debilitating infection that should be treated. PLoS Med 19: e1001942.
-
15.
Bouyou-Akotet MK, Tshibola ML, Mawili-Mboumba DP, Nzong J, Bahamontes-Rosa N, Tsoumbou-Bakana G, Kombila M, 2015. Frequencies of dhfr/dhps multiple mutations and Plasmodium falciparum submicroscopic gametocyte carriage in Gabonese pregnant women following IPTp-SP implementation. Acta Parasitol 60: 2.
-
16.
Mwingira F, Genton B, Kabanywanyi ANM, Felger I, 2014. Comparison of detection methods to estimate asexual Plasmodium falciparum parasite prevalence and gametocyte carriage in a community survey in Tanzania. Malar J 13: 433.
-
17.
Ouédraogo AL, Schneider P, de Kruijf M, Nébié I, Verhave JP, Cuzin-Ouattara N, Sauerwein RW, 2007. Age-dependent distribution of Plasmodium falciparum gametocytes quantified by Pfs25 real-time QT-NASBA in a cross-sectional study in Burkina Faso. Am J Trop Med Hyg 76: 626–630.
-
18.
Manjurano A, Okell L, Lukindo T, Reyburn H, Olomi R, Roper C, Clark TG, Joseph S, Riley EM, Drakeley C, 2011. Association of sub-microscopic malaria parasite carriage with transmission intensity in north-eastern Tanzania. Malar J 10: 370.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 53 | 52 | 12 |
| Full Text Views | 294 | 95 | 1 |
| PDF Downloads | 105 | 33 | 0 |