Author:
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Keusch GT, Denno DM, Black RE, Duggan C, Guerrant RL, Lavery JV, Nataro JP, Rosenberg IH, Ryan ET, Tarr PI, Ward H, Bhutta ZA, Coovadia H, Lima A, Ramakrishna B, Zaidi AK, Hay Burgess DC, Brewer T, 2014. Environmental enteric dysfunction: pathogenesis, diagnosis, and clinical consequences. Clin Infect Dis 59 (Suppl 4): S207–S212.
-
2.
Prendergast AJ, Kelly P, 2016. Interactions between intestinal pathogens, enteropathy and malnutrition in developing countries. Curr Opin Infect Dis 29: 229–236.
-
3.
Denno DM, VanBuskirk K, Nelson ZC, Musser CA, Hay Burgess DC, Tarr PI, 2014. Use of the lactulose to mannitol ratio to evaluate childhood environmental enteric dysfunction: a systematic review. Clin Infect Dis 59 (Suppl 4): S213–S219.
-
4.
Trehan I, Benzoni NS, Wang AZ, Bollinger LB, Ngoma TN, Chimimba UK, Stephenson KB, Agapova SE, Maleta KM, Manary MJ, 2015. Common beans and cowpeas as complementary foods to reduce environmental enteric dysfunction and stunting in Malawian children: study protocol for two randomized controlled trials. Trials 16: 520.
-
5.
Stauber J, Shaikh N, Ordiz MI, Tarr PI, Manary MJ, 2016. Droplet digital PCR quantifies host inflammatory transcripts in feces reliably and reproducibly. Cell Immunol 303: 43–49.
-
6.
Ordiz MI, Shaikh N, Trehan I, Maleta K, Stauber J, Shulman R, Devaraj S, Tarr PI, Manary MJ, 2016. Environmental enteric dysfunction is associated with poor linear growth and can be identified by host fecal mRNAs. J Pediatr Gastroenterol Nutr 63: 453–459.
-
7.
Ordiz MI, May TD, Mihindukulasuriya K, Martin J, Crowley J, Tarr PI, Ryan K, Mortimer E, Gopalsamy G, Maleta K, Mitreva M, Young G, Manary MJ, 2015. The effect of dietary resistant starch type 2 on the microbiota and markers of gut inflammation in rural Malawi children. Microbiome 3: 37.
-
8.
Schloss PD, 2009. A high-throughput DNA sequence aligner for microbial ecology studies. PLoS One 4: e8230.
-
9.
Haas BJ, Gevers D, Earl AM, Feldgarden M, Ward DV, Giannoukos G, Ciulla D, Tabbaa D, Highlander SK, Sodergren E, Methé B, DeSantis TZ; Human Microbiome Consortium, Petrosino JF, Knight R, Birren BW, 2011. Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons. Genome Res 21: 494–504.
-
10.
Cole JR, Wang Q, Cardenas E, Fish J, Chai B, Farris RJ, Kulam-Syed-Mohideen AS, McGarrell DM, Marsh T, Garrity GM, Tiedje JM, 2009. The ribosomal database project: improved alignments and new tools for rRNA analysis. Nucleic Acids Res 37: D141–D145.
-
11.
Oksanen J, Blanchet FG, Kindt R, Legendre P, O'Hara RB, Simpson GL, Solymos P, Henry M, Stevens H, Wagner H, 2016. Vegan: Community Ecology Package 2016. Available at: https://CRAN.R-project.org/package=vegan.
-
12.
Verdu EF, Galipeau HJ, Jabri B, 2015. Novel players in coeliac disease pathogenesis: role of the gut microbiota. Nat Rev Gastroenterol Hepatol 12: 497–506.
-
13.
Graf D, Di Cagno R, Fåk F, Flint HJ, Nyman M, Saarela M, Watzl B, 2015. Contribution of diet to the composition of the human gut microbiota. Microb Ecol Health Dis 26: 26164.
-
14.
Ridlon JM, Kang DJ, Hylemon PB, 2006. Bile salt biotransformations by human intestinal bacteria. J Lipid Res 47: 241–259.
-
15.
Semba RD, Gonzalez-Freire M, Moaddel R, Trehan I, Maleta K, Khadeer M, Ordiz MI, Ferrucci L, Manary MJ, 2016. Environmental enteric dysfunction is associated with altered bile acid metabolism. J Pediatr Gastroenterol Nutr. doi:10.1097/MPG.0000000000001313.
-
16.
Marounek M, Fliegrova K, Bartos S, 1989. Metabolism and some characteristics of ruminal strains of Megasphaera elsdenii. Appl Environ Microbiol 55: 1570–1573.
-
17.
Wacklin P, Kaukinen K, Tuovinen E, Collin P, Lindfors K, Partanen J, Mäki M, Mättö J, 2013. The duodenal microbiota composition of adult celiac disease patients is associated with the clinical manifestation of the disease. Inflamm Bowel Dis 19: 934–941.
-
18.
Vázquez-Castellanos JF, Serrano-Villar S, Latorre A, Artacho A, Ferrús ML, Madrid N, Vallejo A, Sainz T, Martínez-Botas J, Ferrando-Martínez S, Vera M, Dronda F, Leal M, Del Romero J, Moreno S, Estrada V, Gosalbes MJ, Moya A, 2015. Altered metabolism of gut microbiota contributes to chronic immune activation in HIV-infected individuals. Mucosal Immunol 8: 760–772.
-
19.
Levine UY, Bearson SMD, Stanton TB, 2012. Mitsuokella jalaludinii inhibits growth of Salmonella enterica serovar Typhimurium. Veterinarian Microbiology 159: 115–122.
-
20.
Mangin I, Bonnet R, Seksik P, Rigottier-Gois L, Sutren M, Bouhnik Y, Neut C, Collins MD, Colombel JF, Marteau P, Doré J, 2004. Molecular inventory of faecal microflora in patients with Crohn's disease. FEMS Microbiol Ecol 50: 25–36.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 55 | 55 | 9 |
| Full Text Views | 615 | 170 | 2 |
| PDF Downloads | 271 | 54 | 1 |
Environmental Enteric Dysfunction and the Fecal Microbiota in Malawian Children
M. Isabel Ordiz
M. Isabel Ordiz
Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri.
Search for other papers by M. Isabel Ordiz in
Current site
Google Scholar
PubMed
Search for other papers by M. Isabel Ordiz in
Current site
Google Scholar
PubMed
Kevin Stephenson
Kevin Stephenson
Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri.
Search for other papers by Kevin Stephenson in
Current site
Google Scholar
PubMed
Search for other papers by Kevin Stephenson in
Current site
Google Scholar
PubMed
Sophia Agapova
Sophia Agapova
Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri.
Search for other papers by Sophia Agapova in
Current site
Google Scholar
PubMed
Search for other papers by Sophia Agapova in
Current site
Google Scholar
PubMed
Kristine M. Wylie
Kristine M. Wylie
Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri.
The McDonnell Genome Institute, Washington University School of Medicine, St. Louis, Missouri.
Search for other papers by Kristine M. Wylie in
Current site
Google Scholar
PubMed
The McDonnell Genome Institute, Washington University School of Medicine, St. Louis, Missouri.
Search for other papers by Kristine M. Wylie in
Current site
Google Scholar
PubMed
Ken Maleta
Ken Maleta
Department of Community Health, College of Medicine, University of Malawi, Malawi, Africa.
Search for other papers by Ken Maleta in
Current site
Google Scholar
PubMed
Search for other papers by Ken Maleta in
Current site
Google Scholar
PubMed
John Martin
John Martin
The McDonnell Genome Institute, Washington University School of Medicine, St. Louis, Missouri.
Search for other papers by John Martin in
Current site
Google Scholar
PubMed
Search for other papers by John Martin in
Current site
Google Scholar
PubMed
Indi Trehan
Indi Trehan
Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri.
College of Medicine, University of Malawi, Malawi, Africa.
Search for other papers by Indi Trehan in
Current site
Google Scholar
PubMed
College of Medicine, University of Malawi, Malawi, Africa.
Search for other papers by Indi Trehan in
Current site
Google Scholar
PubMed
Phillip I. Tarr
Phillip I. Tarr
Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri.
Search for other papers by Phillip I. Tarr in
Current site
Google Scholar
PubMed
Search for other papers by Phillip I. Tarr in
Current site
Google Scholar
PubMed
Mark J. Manary
Mark J. Manary
Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri.
School of Public Health and Family Medicine, University of Malawi, Blantyre, Malawi.
United States Department of Agriculture/Agricultural Research Service Childrens Nutrition Research Center, Houston, Texas.
Search for other papers by Mark J. Manary in
Current site
Google Scholar
PubMed
School of Public Health and Family Medicine, University of Malawi, Blantyre, Malawi.
United States Department of Agriculture/Agricultural Research Service Childrens Nutrition Research Center, Houston, Texas.
Search for other papers by Mark J. Manary in
Current site
Google Scholar
PubMed
Environmental enteric dysfunction (EED) is often measured with a dual sugar absorption test and implicated as a causative factor in childhood stunting. Disturbances in the gut microbiota are hypothesized to be a mechanism by which EED is exacerbated, although this supposition lacks support. We performed 16S ribosomal RNA gene sequencing of fecal samples from 81 rural Malawian children with varying degrees of EED to determine which bacterial taxa were associated with EED. At the phyla level, Proteobacteria abundance is reduced with severe EED. Among bacterial genera, Megasphaera, Mitsuokella, and Sutterella were higher in EED and Succinivibrio, Klebsiella, and Clostridium_XI were lower in EED. Bacterial diversity did not vary with the extent of EED. Though EED is a condition that is typically believed to affect the proximal small bowel, and our focus was on stool, our data do suggest that there are intraluminal microbial differences that reflect, or plausibly lead to, EED.
Author Notes
Financial support: This study was supported by the Feed the Future Program, USAID, and the Children's Discovery Institute of Washington University and St. Louis Children's Hospital.
Conflict of interest: Phillip I. Tarr is on the Scientific Advisory Board of MediBeacon, and is the coinventor of a novel technology to measure gut permeability.
Authors' addresses: M. Isabel Ordiz, Kevin Stephenson, Sophia Agapova, Kristine M. Wylie, Indi Trehan, Phillip I. Tarr, and Mark J. Manary, Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, E-mails: ordiz_i@wustl.edu, kbstephe@gmail.com, shtepaz@gmail.com, kwylie@wustl.edu, itrehan@wustl.edu, tarr@wustl.edu, and manary@wustl.edu. John Martin, The McDonnell Genome Institute, St. Louis, MO, E-mail: jmartin@wustl.edu. Ken Maleta, Department of Community Health, College of Medicine, University of Malawi, Malawi, Africa, E-mail: ken.maleta@gmail.com.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Keusch GT, Denno DM, Black RE, Duggan C, Guerrant RL, Lavery JV, Nataro JP, Rosenberg IH, Ryan ET, Tarr PI, Ward H, Bhutta ZA, Coovadia H, Lima A, Ramakrishna B, Zaidi AK, Hay Burgess DC, Brewer T, 2014. Environmental enteric dysfunction: pathogenesis, diagnosis, and clinical consequences. Clin Infect Dis 59 (Suppl 4): S207–S212.
-
2.
Prendergast AJ, Kelly P, 2016. Interactions between intestinal pathogens, enteropathy and malnutrition in developing countries. Curr Opin Infect Dis 29: 229–236.
-
3.
Denno DM, VanBuskirk K, Nelson ZC, Musser CA, Hay Burgess DC, Tarr PI, 2014. Use of the lactulose to mannitol ratio to evaluate childhood environmental enteric dysfunction: a systematic review. Clin Infect Dis 59 (Suppl 4): S213–S219.
-
4.
Trehan I, Benzoni NS, Wang AZ, Bollinger LB, Ngoma TN, Chimimba UK, Stephenson KB, Agapova SE, Maleta KM, Manary MJ, 2015. Common beans and cowpeas as complementary foods to reduce environmental enteric dysfunction and stunting in Malawian children: study protocol for two randomized controlled trials. Trials 16: 520.
-
5.
Stauber J, Shaikh N, Ordiz MI, Tarr PI, Manary MJ, 2016. Droplet digital PCR quantifies host inflammatory transcripts in feces reliably and reproducibly. Cell Immunol 303: 43–49.
-
6.
Ordiz MI, Shaikh N, Trehan I, Maleta K, Stauber J, Shulman R, Devaraj S, Tarr PI, Manary MJ, 2016. Environmental enteric dysfunction is associated with poor linear growth and can be identified by host fecal mRNAs. J Pediatr Gastroenterol Nutr 63: 453–459.
-
7.
Ordiz MI, May TD, Mihindukulasuriya K, Martin J, Crowley J, Tarr PI, Ryan K, Mortimer E, Gopalsamy G, Maleta K, Mitreva M, Young G, Manary MJ, 2015. The effect of dietary resistant starch type 2 on the microbiota and markers of gut inflammation in rural Malawi children. Microbiome 3: 37.
-
8.
Schloss PD, 2009. A high-throughput DNA sequence aligner for microbial ecology studies. PLoS One 4: e8230.
-
9.
Haas BJ, Gevers D, Earl AM, Feldgarden M, Ward DV, Giannoukos G, Ciulla D, Tabbaa D, Highlander SK, Sodergren E, Methé B, DeSantis TZ; Human Microbiome Consortium, Petrosino JF, Knight R, Birren BW, 2011. Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons. Genome Res 21: 494–504.
-
10.
Cole JR, Wang Q, Cardenas E, Fish J, Chai B, Farris RJ, Kulam-Syed-Mohideen AS, McGarrell DM, Marsh T, Garrity GM, Tiedje JM, 2009. The ribosomal database project: improved alignments and new tools for rRNA analysis. Nucleic Acids Res 37: D141–D145.
-
11.
Oksanen J, Blanchet FG, Kindt R, Legendre P, O'Hara RB, Simpson GL, Solymos P, Henry M, Stevens H, Wagner H, 2016. Vegan: Community Ecology Package 2016. Available at: https://CRAN.R-project.org/package=vegan.
-
12.
Verdu EF, Galipeau HJ, Jabri B, 2015. Novel players in coeliac disease pathogenesis: role of the gut microbiota. Nat Rev Gastroenterol Hepatol 12: 497–506.
-
13.
Graf D, Di Cagno R, Fåk F, Flint HJ, Nyman M, Saarela M, Watzl B, 2015. Contribution of diet to the composition of the human gut microbiota. Microb Ecol Health Dis 26: 26164.
-
14.
Ridlon JM, Kang DJ, Hylemon PB, 2006. Bile salt biotransformations by human intestinal bacteria. J Lipid Res 47: 241–259.
-
15.
Semba RD, Gonzalez-Freire M, Moaddel R, Trehan I, Maleta K, Khadeer M, Ordiz MI, Ferrucci L, Manary MJ, 2016. Environmental enteric dysfunction is associated with altered bile acid metabolism. J Pediatr Gastroenterol Nutr. doi:10.1097/MPG.0000000000001313.
-
16.
Marounek M, Fliegrova K, Bartos S, 1989. Metabolism and some characteristics of ruminal strains of Megasphaera elsdenii. Appl Environ Microbiol 55: 1570–1573.
-
17.
Wacklin P, Kaukinen K, Tuovinen E, Collin P, Lindfors K, Partanen J, Mäki M, Mättö J, 2013. The duodenal microbiota composition of adult celiac disease patients is associated with the clinical manifestation of the disease. Inflamm Bowel Dis 19: 934–941.
-
18.
Vázquez-Castellanos JF, Serrano-Villar S, Latorre A, Artacho A, Ferrús ML, Madrid N, Vallejo A, Sainz T, Martínez-Botas J, Ferrando-Martínez S, Vera M, Dronda F, Leal M, Del Romero J, Moreno S, Estrada V, Gosalbes MJ, Moya A, 2015. Altered metabolism of gut microbiota contributes to chronic immune activation in HIV-infected individuals. Mucosal Immunol 8: 760–772.
-
19.
Levine UY, Bearson SMD, Stanton TB, 2012. Mitsuokella jalaludinii inhibits growth of Salmonella enterica serovar Typhimurium. Veterinarian Microbiology 159: 115–122.
-
20.
Mangin I, Bonnet R, Seksik P, Rigottier-Gois L, Sutren M, Bouhnik Y, Neut C, Collins MD, Colombel JF, Marteau P, Doré J, 2004. Molecular inventory of faecal microflora in patients with Crohn's disease. FEMS Microbiol Ecol 50: 25–36.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 55 | 55 | 9 |
| Full Text Views | 615 | 170 | 2 |
| PDF Downloads | 271 | 54 | 1 |