ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Tan KSW, 2008. New insights on classification, identification, and clinical relevance of Blastocystis spp. Clin Microbiol Rev 21: 639–665.
-
2.
Clark CG, van der Giezen M, Alfellani MA, Stensvold CR, 2013. Recent developments in Blastocystis research. Adv Parasitol 82: 1–32.
-
3.
Scanlan PD, Stensvold CR, Rajilić-Stojanović M, Heilig HG, De Vos WM, O'Toole PW, Cotter PD, 2014. The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota. FEMS Microbiol Ecol 90: 326–330.
-
4.
El Safadi D, Gaayeb L, Meloni D, Cian A, Poirier P, Wawrzyniak I, Delbac F, Dabboussi F, Delhaes L, Seck M, Hamze M, Riveau G, Viscogliosi E, 2014. Children of Senegal River Basin show the highest prevalence of Blastocystis sp. ever observed worldwide. BMC Infect Dis 14: 164.
-
5.
Poirier P, Wawrzyniak I, Albert A, El Alaoiu H, Delbac F, Livrelli V, 2011. Development and evaluation of a real-time PCR assay for detection and quantification of Blastocystis parasites in human stool samples: prospective study of patients with hematological malignancies. J Clin Microbiol 49: 975–983.
-
6.
Andersen LO, Stensvold CR, 2016. Blastocystis in health and disease: are we moving from a clinical to a public health perspective? J Clin Microbiol 54: 524–528.
-
7.
Ajjampur SSR, Tan KSW, 2016. Pathogenic mechanisms in Blastocystis spp.—interpreting results from in vitro and in vivo studies. Parasitol Int 65: 772–779.
-
8.
Lepczynska M, Chen WC, Dzika E, 2016. Mysterious chronic urticarial caused by Blastocystis spp.? Int J Dermatol 55: 259–266.
-
9.
Poirier P, Wawrzyniak I, Vivarès CP, Delbac F, El Alaoui H, 2012. New insights into Blastocystis spp.: a potential link with irritable bowel syndrome. PLoS Pathog 8: e1002545.
-
10.
Alfellani MA, Taner-Mulla D, Jacob AS, Imeede CA, Yoshikawa H, Stensvold CR, Clark CG, 2013. Genetic diversity of Blastocystis in livestock and zoo animals. Protist 164: 497–509.
-
11.
Alfellani MA, Stensvold CR, Vidal-Lapiedra A, Onuoha ES, Fagbenro-Beyioku AF, Clark CG, 2013. Variable geographic distribution of Blastocystis subtypes and its potential implications. Acta Trop 126: 11–18.
-
12.
Siala E, Guidara R, Ben Abdallah R, Ben Ayed S, Ben Alaya N, Zallaga N, Bouratbine A, Aoun K, 2011. The intestinal parasites in the food handlers of Tunis area: study of 8502 stool samples (1998–2008). Arch Inst Pasteur Tunis 88: 77–84.
-
13.
Trabelsi S, Ali IB, Khaled S, 2010. Clinical and epidemiological characteristics of Blastocystis hominis. Tunis Med 88: 190–192.
-
14.
Benouis A, Bekkouche Z, Benmansour Z, 2013. Epidemiological study of human intestinal parasitosis in the Hospital of Oran (Algeria). Int J Innov Appl Studies 2: 613–620.
-
15.
Tligui H, Oudghiri M, Bahji M, Agoumi A, 2001. Données épidémiologiques et pathologiques sur Blastocystis hominis. Rev Mar Biol Infect 10: 13–18.
-
16.
El Guamri Y, Belghyti D, Barkia A, Tiabi M, Aujjar N, Achicha A, El Kharrim K, Elfellaki L, 2011. Parasitic infection of the digestive tract in children in a regional hospital center in Gharb (Kenitra, Morocco): some epidemiological features. East Afr J Public Health 8: 250–257.
-
17.
El Fatni C, Olmo F, El Fatni H, Romero D, Rosales MJ, 2014. First genotyping of Giardia duodenalis and prevalence of enteroparasites in children from Tetouan (Morocco). Parasite 21: 48.
-
18.
Al-Fellani MA, Khan AH, Al-Gazoui RM, Zaid MK, Al-Ferjani MA, 2007. Prevalence and clinical features of Blastocystis hominis infection among patients in Sebha, Libya. Sultan Qaboos Univ Med J 7: 35–40.
-
19.
Abdulsalam AM, Ithoi I, Al-Mekhlafi HM, Al-Mekhlafi AM, Ahmed A, Surin J, 2013. Subtype distribution of Blastocystis isolates in Sebha, Libya. PLoS One 8: e84372.
-
20.
Hussein EM, Hussein AM, Eida MM, Atwa MM, 2008. Pathophysiological variability of different genotypes of human Blastocystis hominis Egyptian isolates in experimentally infected rats. Parasitol Res 102: 853–860.
-
21.
Souppart L, Moussa H, Cian A, Sanciu G, Poirier P, El Alaoui H, Delbac F, Boorom K, Delhaes L, Dei-Cas E, Viscogliosi E, 2010. Subtype analysis of Blastocystis isolates from symptomatic patients in Egypt. Parasitol Res 106: 505–511.
-
22.
Fouad SA, Basyoni MM, Fahmy RA, Kobaisi MH, 2011. The pathogenic role of different Blastocystis hominis genotypes isolated from patients with irritable bowel syndrome. Arab J Gastroenterol 12: 194–200.
-
23.
Essid R, Mousli M, Aoun K, Abdelmalek R, Mellouli F, Kanoun F, Derouin F, Bouratbine A, 2008. Identification of Cryptosporidium species infecting humans in Tunisia. Am J Trop Med Hyg 79: 702–705.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1507 | 1401 | 35 |
| Full Text Views | 390 | 13 | 0 |
| PDF Downloads | 114 | 18 | 0 |
Prevalence and Subtype Identification of Blastocystis sp. in Healthy Individuals in the Tunis Area, Tunisia
Imène Ben Abda
Imène Ben Abda
Research Laboratory of Medical Parasitology, Biotechnology and Biomolecules (PMBB), Institut Pasteur of Tunis, Tunis, Tunisia.
Department of Parasitology, Institut Pasteur of Tunis, Tunis, Tunisia.
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Department of Parasitology, Institut Pasteur of Tunis, Tunis, Tunisia.
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Nabil Maatoug
Nabil Maatoug
Research Laboratory of Medical Parasitology, Biotechnology and Biomolecules (PMBB), Institut Pasteur of Tunis, Tunis, Tunisia.
Department of Parasitology, Institut Pasteur of Tunis, Tunis, Tunisia.
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Department of Parasitology, Institut Pasteur of Tunis, Tunis, Tunisia.
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Rania Ben Romdhane
Rania Ben Romdhane
Research Laboratory of Medical Parasitology, Biotechnology and Biomolecules (PMBB), Institut Pasteur of Tunis, Tunis, Tunisia.
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Nada Bouhelmi
Nada Bouhelmi
Department of Parasitology, Institut Pasteur of Tunis, Tunis, Tunisia.
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Najet Zallegua
Najet Zallegua
Department of Parasitology, Institut Pasteur of Tunis, Tunis, Tunisia.
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Karim Aoun
Karim Aoun
Research Laboratory of Medical Parasitology, Biotechnology and Biomolecules (PMBB), Institut Pasteur of Tunis, Tunis, Tunisia.
Department of Parasitology, Institut Pasteur of Tunis, Tunis, Tunisia.
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Department of Parasitology, Institut Pasteur of Tunis, Tunis, Tunisia.
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Eric Viscogliosi
Eric Viscogliosi
Centre d'Infection et d'Immunité de Lille, Institut Pasteur de Lille, Université de Lille, Lille, France.
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Aïda Bouratbine
Aïda Bouratbine
Research Laboratory of Medical Parasitology, Biotechnology and Biomolecules (PMBB), Institut Pasteur of Tunis, Tunis, Tunisia.
Department of Parasitology, Institut Pasteur of Tunis, Tunis, Tunisia.
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Department of Parasitology, Institut Pasteur of Tunis, Tunis, Tunisia.
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Blastocystis sp. is currently the most common eukaryotic parasite found in humans. Despite its potential public health impact, epidemiological data regarding its prevalence and molecular subtype (ST) distribution in Maghreb are rarely reported. Therefore, the aim of this study was to determine the prevalence of the parasite in a cohort of healthy food handler Tunisian individuals and to acquire the first molecular data regarding the distribution of Blastocystis sp. STs in this country. Therefore, 524 fecal samples were collected, and 68 of them (13%) were identified as positive for the parasite by direct-light microscopy of smears. Seventeen samples of 100 negative by microscopy were also shown to be positive by real-time quantitative polymerase chain reaction. Among all the positive samples, 61 Blastocystis isolates were subtyped using partial small subunit ribosomal RNA gene analysis. ST3 was the most abundant (51%) followed by ST1 (30%), ST2 (16%), and ST4 and ST7 (both 1.6%).
Author Notes
Financial support: This study was supported by the Ministry of Higher Education and Scientific Research, Tunisia, in the setting of the Research Laboratory of Medical Parasitology, Biotechnology and Biomolecules (PMBB), and Institut Pasteur of Tunis, Tunisia (LR11-IPT06).
Authors' addresses: Imène Ben Abda, Nabil Maatoug, Rania Ben Romdhane, Nada Bouhelmi, Najet Zallegua, Karim Aoun, and Aïda Bouratbine, Laboratoire de Parasitologie, Institut Pasteur de Tunis, Tunis, Tunisia, E-mails: benabda.imen@hotmail.fr, nabilmaatoug2@gmail.com, rania.bromdhane91@gmail.com, nadabouhelmi@gmail.com, najet.zallaga@pasteur.rns.tn, karim.aoun@pasteur.rns.tn, and aida.bouratbine@pasteur.rns.tn. Eric Viscogliosi, Centre d'Infection et d'Immunité de Lille, Institut Pasteur de Lille, Université de Lille, Lille, France, E-mail: eric.viscogliosi@pasteur-lille.fr.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Tan KSW, 2008. New insights on classification, identification, and clinical relevance of Blastocystis spp. Clin Microbiol Rev 21: 639–665.
-
2.
Clark CG, van der Giezen M, Alfellani MA, Stensvold CR, 2013. Recent developments in Blastocystis research. Adv Parasitol 82: 1–32.
-
3.
Scanlan PD, Stensvold CR, Rajilić-Stojanović M, Heilig HG, De Vos WM, O'Toole PW, Cotter PD, 2014. The microbial eukaryote Blastocystis is a prevalent and diverse member of the healthy human gut microbiota. FEMS Microbiol Ecol 90: 326–330.
-
4.
El Safadi D, Gaayeb L, Meloni D, Cian A, Poirier P, Wawrzyniak I, Delbac F, Dabboussi F, Delhaes L, Seck M, Hamze M, Riveau G, Viscogliosi E, 2014. Children of Senegal River Basin show the highest prevalence of Blastocystis sp. ever observed worldwide. BMC Infect Dis 14: 164.
-
5.
Poirier P, Wawrzyniak I, Albert A, El Alaoiu H, Delbac F, Livrelli V, 2011. Development and evaluation of a real-time PCR assay for detection and quantification of Blastocystis parasites in human stool samples: prospective study of patients with hematological malignancies. J Clin Microbiol 49: 975–983.
-
6.
Andersen LO, Stensvold CR, 2016. Blastocystis in health and disease: are we moving from a clinical to a public health perspective? J Clin Microbiol 54: 524–528.
-
7.
Ajjampur SSR, Tan KSW, 2016. Pathogenic mechanisms in Blastocystis spp.—interpreting results from in vitro and in vivo studies. Parasitol Int 65: 772–779.
-
8.
Lepczynska M, Chen WC, Dzika E, 2016. Mysterious chronic urticarial caused by Blastocystis spp.? Int J Dermatol 55: 259–266.
-
9.
Poirier P, Wawrzyniak I, Vivarès CP, Delbac F, El Alaoui H, 2012. New insights into Blastocystis spp.: a potential link with irritable bowel syndrome. PLoS Pathog 8: e1002545.
-
10.
Alfellani MA, Taner-Mulla D, Jacob AS, Imeede CA, Yoshikawa H, Stensvold CR, Clark CG, 2013. Genetic diversity of Blastocystis in livestock and zoo animals. Protist 164: 497–509.
-
11.
Alfellani MA, Stensvold CR, Vidal-Lapiedra A, Onuoha ES, Fagbenro-Beyioku AF, Clark CG, 2013. Variable geographic distribution of Blastocystis subtypes and its potential implications. Acta Trop 126: 11–18.
-
12.
Siala E, Guidara R, Ben Abdallah R, Ben Ayed S, Ben Alaya N, Zallaga N, Bouratbine A, Aoun K, 2011. The intestinal parasites in the food handlers of Tunis area: study of 8502 stool samples (1998–2008). Arch Inst Pasteur Tunis 88: 77–84.
-
13.
Trabelsi S, Ali IB, Khaled S, 2010. Clinical and epidemiological characteristics of Blastocystis hominis. Tunis Med 88: 190–192.
-
14.
Benouis A, Bekkouche Z, Benmansour Z, 2013. Epidemiological study of human intestinal parasitosis in the Hospital of Oran (Algeria). Int J Innov Appl Studies 2: 613–620.
-
15.
Tligui H, Oudghiri M, Bahji M, Agoumi A, 2001. Données épidémiologiques et pathologiques sur Blastocystis hominis. Rev Mar Biol Infect 10: 13–18.
-
16.
El Guamri Y, Belghyti D, Barkia A, Tiabi M, Aujjar N, Achicha A, El Kharrim K, Elfellaki L, 2011. Parasitic infection of the digestive tract in children in a regional hospital center in Gharb (Kenitra, Morocco): some epidemiological features. East Afr J Public Health 8: 250–257.
-
17.
El Fatni C, Olmo F, El Fatni H, Romero D, Rosales MJ, 2014. First genotyping of Giardia duodenalis and prevalence of enteroparasites in children from Tetouan (Morocco). Parasite 21: 48.
-
18.
Al-Fellani MA, Khan AH, Al-Gazoui RM, Zaid MK, Al-Ferjani MA, 2007. Prevalence and clinical features of Blastocystis hominis infection among patients in Sebha, Libya. Sultan Qaboos Univ Med J 7: 35–40.
-
19.
Abdulsalam AM, Ithoi I, Al-Mekhlafi HM, Al-Mekhlafi AM, Ahmed A, Surin J, 2013. Subtype distribution of Blastocystis isolates in Sebha, Libya. PLoS One 8: e84372.
-
20.
Hussein EM, Hussein AM, Eida MM, Atwa MM, 2008. Pathophysiological variability of different genotypes of human Blastocystis hominis Egyptian isolates in experimentally infected rats. Parasitol Res 102: 853–860.
-
21.
Souppart L, Moussa H, Cian A, Sanciu G, Poirier P, El Alaoui H, Delbac F, Boorom K, Delhaes L, Dei-Cas E, Viscogliosi E, 2010. Subtype analysis of Blastocystis isolates from symptomatic patients in Egypt. Parasitol Res 106: 505–511.
-
22.
Fouad SA, Basyoni MM, Fahmy RA, Kobaisi MH, 2011. The pathogenic role of different Blastocystis hominis genotypes isolated from patients with irritable bowel syndrome. Arab J Gastroenterol 12: 194–200.
-
23.
Essid R, Mousli M, Aoun K, Abdelmalek R, Mellouli F, Kanoun F, Derouin F, Bouratbine A, 2008. Identification of Cryptosporidium species infecting humans in Tunisia. Am J Trop Med Hyg 79: 702–705.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1507 | 1401 | 35 |
| Full Text Views | 390 | 13 | 0 |
| PDF Downloads | 114 | 18 | 0 |