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Evans BR, Gloria-Soria A, Hou L, McBride C, Bonizzoni M, Zhao H, Powell JR, 2015. A multipurpose high-throughput SNP chip for the dengue and yellow fever mosquito, Aedes aegypti. G3 (Bethesda) 5: 711–718.
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2.
Matthews TC, Craig GB, 1987. Heterozygosity in inbred strains of the tree-hole mosquito Aedes triseriatus. Biochem Genet 25: 647–655.
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3.
Matthews TC, Craig GB, 1989. Isozyme polymorphisms maintained by lethal loci in inbred strains of Aedes triseriatus. J Hered 80: 53–57.
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4.
Munstermann LE, 1994. Unexpected consequences of colonization and inbreeding—allozyme training in Culicidae (Diptera). Ann Entomol Soc Am 87: 157–164.
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5.
Gomez-Machorro C, Bennett KE, del Lourdes Munoz M, Black WC, 2004. Quantitative trait loci affecting dengue midgut infection barriers in an advanced intercross line of Aedes aegypti. Insect Mol Biol 13: 637–648.
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How Much Does Inbreeding Reduce Heterozygosity? Empirical Results from Aedes aegypti
Jeffrey R. Powell
Jeffrey R. Powell
Yale University, New Haven, Connecticut.
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Benjamin R. Evans
Benjamin R. Evans
Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut.
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Deriving strains of mosquitoes with reduced genetic variation is useful, if not necessary, for many genetic studies. Inbreeding is the standard way of achieving this. Full-sib inbreeding the mosquito Aedes aegypti for seven generations reduced heterozygosity to 72% of the initial heterozygosity in contrast to the expected 13%. This deviation from expectations is likely due to high frequencies of deleterious recessive alleles that, given the number of markers studied (27,674 single nucleotide polymorphisms [SNPs]), must be quite densely spread in the genome.
Author Notes
Financial support: This study was supported by NIH NIAID RO1 AI101112 and NIH 5T32 AI007404.
Authors' addresses: Jeffrey R. Powell, Yale University, New Haven, CT, E-mail: jeffrey.powell@yale.edu. Benjamin R. Evans, Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT, E-mail: b.evans@yale.edu.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Evans BR, Gloria-Soria A, Hou L, McBride C, Bonizzoni M, Zhao H, Powell JR, 2015. A multipurpose high-throughput SNP chip for the dengue and yellow fever mosquito, Aedes aegypti. G3 (Bethesda) 5: 711–718.
-
2.
Matthews TC, Craig GB, 1987. Heterozygosity in inbred strains of the tree-hole mosquito Aedes triseriatus. Biochem Genet 25: 647–655.
-
3.
Matthews TC, Craig GB, 1989. Isozyme polymorphisms maintained by lethal loci in inbred strains of Aedes triseriatus. J Hered 80: 53–57.
-
4.
Munstermann LE, 1994. Unexpected consequences of colonization and inbreeding—allozyme training in Culicidae (Diptera). Ann Entomol Soc Am 87: 157–164.
-
5.
Gomez-Machorro C, Bennett KE, del Lourdes Munoz M, Black WC, 2004. Quantitative trait loci affecting dengue midgut infection barriers in an advanced intercross line of Aedes aegypti. Insect Mol Biol 13: 637–648.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 35 | 35 | 8 |
| Full Text Views | 299 | 91 | 0 |
| PDF Downloads | 112 | 29 | 0 |