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-
1.
Aderinboye O, Syed SS, 2010. Congenital babesiosis in a four-week-old female infant. Pediatr Infect Dis J 29: 188.
-
2.
Herwaldt BL, Linden JV, Bosserman E, Young C, Olkowska D, Wilson M, 2011. Transfusion-associated babesiosis in the United States: a description of cases. Ann Intern Med 155: 509–519.
-
3.
Scholtens RG, Braff EH, Healey GA, Gleason N, 1968. A case of babesiosis in man in the United States. Am J Trop Med Hyg 17: 810–813.
-
4.
Centers for Disease Control and Prevention, 2012. Babesiosis surveillance: 18 States, 2011. Morb Mortal Wkly Rep 61: 505–509.
-
5.
Garcia LS, 2001. Procedures for Detecting Blood Parasites. Diagnostic Medical Parasitology. Washington, DC: ASM Press, 829–849.
-
6.
Quick RE, Herwaldt BL, Thomford JW, Garnett ME, Eberhard ML, Wilson M, Spach DH, Dickerson JW, Telford SR 3rd, Steingart KR, Pollock R, Persing DH, Kobayashi JM, Juranek DD, Conrad PA, 1993. Babesiosis in Washington State: a new species of Babesia? Ann Intern Med 119: 284–290.
-
7.
Vannier E, Gewurz BE, Krause PJ, 2008. Human babesiosis. Infect Dis Clin North Am 22: 469–488, viii–ix.
-
8.
Conrad PA, Kjemtrup AM, Carreno RA, Thomford J, Wainwright K, Eberhard M, Quick R, Telford SR 3rd, Herwaldt BL, 2006. Description of Babesia duncani n.sp. (Apicomplexa: Babesiidae) from humans and its differentiation from other piroplasms. Int J Parasitol 36: 779–789.
-
9.
Herwaldt B, Persing DH, Precigout EA, Goff WL, Mathiesen DA, Taylor PW, Eberhard ML, Gorenflot AF, 1996. A fatal case of babesiosis in Missouri: identification of another piroplasm that infects humans. Ann Intern Med 124: 643–650.
-
10.
Persing DH, Mathiesen D, Marshall WF, Telford SR, Spielman A, Thomford JW, Conrad PA, 1992. Detection of Babesia microti by polymerase chain reaction. J Clin Microbiol 30: 2097–2103.
-
11.
Bonnet S, Jouglin M, L'Hostis M, Chauvin A, 2007. Babesia sp. EU1 from roe deer and transmission within Ixodes ricinus. Emerg Infect Dis 13: 1208–1210.
-
12.
Bloch EM, Herwaldt BL, Leiby DA, Shaieb A, Herron RM, Chervenak M, Reed W, Hunter R, Ryals R, Hagar W, Xayavong MV, Slemenda SB, Pieniazek NJ, Wilkins PP, Kjemtrup AM, 2012. The third described case of transfusion-transmitted Babesia duncani. Transfusion 52: 1517–1522.
-
13.
Herwaldt BL, de Bruyn G, Pieniazek NJ, Homer M, Lofy KH, Slemenda SB, Fritsche TR, Persing DH, Limaye AP, 2004. Babesia divergens-like infection, Washington State. Emerg Infect Dis 10: 622–629.
-
14.
Qvarnstrom Y, Schijman AG, Veron V, Aznar C, Steurer F, da Silva AJ, 2012. Sensitive and specific detection of Trypanosoma cruzi DNA in clinical specimens using a multi-target real-time PCR approach. PLoS Negl Trop Dis 6: e1689.
-
15.
Rougemont M, Van Saanen M, Sahli R, Hinrikson HP, Bille J, Jaton K, 2004. Detection of four Plasmodium species in blood from humans by 18S rRNA gene subunit-based and species-specific real-time PCR assays. J Clin Microbiol 42: 5636–5643.
-
16.
Purnell RE, Brocklesby DW, Hendry DJ, Young ER, 1976. Separation and recombination of Babesia divergens and Ehrlichia phagocytophila from a field case of redwater from Eire. Vet Rec 99: 415–417.
-
17.
Bloch EM, Lee TH, Krause PJ, Telford SR 3rd, Montalvo L, Chafets D, Usmani-Brown S, Lepore TJ, Busch MP, 2013. Development of a real-time polymerase chain reaction assay for sensitive detection and quantitation of Babesia microti infection. Transfusion 53: 2299–2306.
-
18.
Bown KJ, Lambin X, Telford GR, Ogden NH, Telfer S, Woldehiwet Z, Birtles RJ, 2008. Relative importance of Ixodes ricinus and Ixodes trianguliceps as vectors for Anaplasma phagocytophilum and Babesia microti in field vole (Microtus agrestis) populations. Appl Environ Microbiol 74: 7118–7125.
-
19.
Chan K, Marras SA, Parveen N, 2013. Sensitive multiplex PCR assay to differentiate Lyme spirochetes and emerging pathogens Anaplasma phagocytophilum and Babesia microti. BMC Microbiol 13: 295.
-
20.
Hersh MH, Tibbetts M, Strauss M, Ostfeld RS, Keesing F, 2012. Reservoir competence of wildlife host species for Babesia microti. Emerg Infect Dis 18: 1951–1957.
-
21.
Hojgaard A, Lukacik G, Piesman J, 2014. Detection of Borrelia burgdorferi, Anaplasma phagocytophilum, and Babesia microti, with two different multiplex PCR assays. Ticks Tick Borne Dis 5: 349–351.
-
22.
Rollend L, Bent SJ, Krause PJ, Usmani-Brown S, Steeves TK, States SL, Lepore T, Ryan R, Dias F, Ben Mamoun C, Fish D, Diuk-Wasser MA, 2013. Quantitative PCR for detection of Babesia microti in Ixodes scapularis ticks and in human blood. Vector Borne Zoonotic Dis 13: 784–790.
-
23.
Teal AE, Habura A, Ennis J, Keithly JS, Madison-Antenucci S, 2012. A new real-time PCR assay for improved detection of the parasite Babesia microti. J Clin Microbiol 50: 903–908.
-
24.
Tonnetti L, Eder AF, Dy B, Kennedy J, Pisciotto P, Benjamin RJ, Leiby DA, 2009. Transfusion-transmitted Babesia microti identified through hemovigilance. Transfusion 49: 2557–2563.
-
25.
Cullen JM, Levine JF, 1987. Pathology of experimental Babesia microti infection in the Syrian hamster. Lab Anim Sci 37: 640–643.
-
26.
Newcombe RG, 1998. Two-sided confidence intervals for the single proportion: comparison of seven methods. Stat Med 17: 857–872.
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Comparison of Babesia microti Real-Time Polymerase Chain Reaction Assays for Confirmatory Diagnosis of Babesiosis
Abstract
Babesiosis is an emerging tick-borne disease caused by apicomplexan parasites of the genus Babesia. Most human infections in the United States are caused by Babesia microti, but other infection-causing Babesia parasites have been documented as well. Polymerase chain reaction (PCR)–based methods can be used to identify this parasite to the species level. In this study, published real-time PCR assays for the specific detection of B. microti were evaluated against conventional PCR for their analytical performance. All evaluated real-time PCR assays had comparable dynamic range and amplification efficiency, but the sensitivity and specificity varied. The best performing test, a TaqMan assay targeting the 18S ribosomal RNA gene, was further evaluated for diagnostic performance using blood specimens submitted to the Centers for Disease Control and Prevention for parasite detection and was found to have 100% sensitivity and specificity. In conclusion, the 18S TaqMan real-time PCR assay is a sensitive, specific, and rapid method for identification of B. microti among cases of babesiosis in the United States.
Author Notes
Authors' addresses: Samaly S. Souza, Henry S. Bishop, Patrick Sprinkle, and Yvonne Qvarnstrom, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, GA, E-mails: samalysouza@gmail.com, hsb2@cdc.gov, grc4@cdc.gov, and bvp2@cdc.gov.