Nucleolar Persistence: Peculiar Characteristic of Spermatogenesis of the Vectors of Chagas Disease (Hemiptera, Triatominae)

Fernanda Fernandez Madeira Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista–São José do Rio Preto, São Paulo, Brazil.

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Kelly Cristine Borsatto Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista–São José do Rio Preto, São Paulo, Brazil.

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Anna Claudia Campaner Lima Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista–São José do Rio Preto, São Paulo, Brazil.

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Amanda Ravazi Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista–São José do Rio Preto, São Paulo, Brazil.

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Jader de Oliveira Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista–Araraquara, São Paulo, Brazil.

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João Aristeu da Rosa Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista–Araraquara, São Paulo, Brazil.

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Maria Tercília Vilela de Azeredo-Oliveira Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista–São José do Rio Preto, São Paulo, Brazil.

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Kaio Cesar Chaboli Alevi Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista–São José do Rio Preto, São Paulo, Brazil.

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All species of triatomines are considered potential vectors of Chagas disease and the reproductive biology of these bugs has been studied by different approaches. In 1999, nucleolar persistence during meiosis was observed in the subfamily for the first time. Recently, it has been observed that all species within the genus Rhodnius exhibit the same phenomenon, suggesting that it may be a synapomorphy of the triatomines. Thus, this article aims to analyze the nucleolar behavior during spermatogenesis of 59 triatomine species. All analyzed species exhibited nucleolar persistence during meiosis. Recently, it has been suggested that nucleolar persistence may be fundamental for the spermatogenesis of these vectors, since it is related to the formation of the chromatoid body. Therefore, we emphasize that this phenomenon is a peculiarity of the Triatominae subfamily and that further studies are required to analyze whether the nucleolar material that persists is active.

Author Notes

* Address correspondence to Kaio Cesar Chaboli Alevi, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista (IBILCE–UNESP), Rua Cristovão Colombo, 2265, Jardim Nazareth, CEP 15054-000, São José do Rio Preto, São Paulo, Brazil. E-mail: kaiochaboli@hotmail.com

Financial support: The study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (process numbers 2013/19764-0 and 2015/14762-5-FAPESP, Brazil) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil).

Authors' addresses: Fernanda Fernandez Madeira, Kelly Cristine Borsatto, Anna Claudia Campaner Lima, Amanda Ravazi, Maria Tercília Vilela Azeredo-Oliveira, and Kaio Cesar Chaboli Alevi, Departamento de Biologia, Instituto de Biociencias, Letras e Ciencias Exatas, Universidade Estadual Paulista Julio de Mesquita Filho–Campus de Sao Jose do Rio Preto, São Paulo, Brazil, E-mails: fernanda.bio56@hotmail.com, kellyborsatto@gmail.com, anna_campaner@hotmail.com, amandaravazi95@gmail.com, tercilia@ibilce.unesp.br, and kaiochaboli@hotmail.com. Jader de Oliveira and João Aristeu da Rosa, Departamento de Ciências Biológicas, Faculdade de Ciencias Farmaceuticas, Universidade Estadual Paulista Julio de Mesquita Filho–Campus de Araraquara, São Paulo, Brazil, E-mails: jdr.oliveira@hotmail.com and joaoaristeu@gmail.com.

  • 1.

    World Health Organization, 2015. Chagas disease (American trypanosomiasis). Wkly Epidemiol Rec 90: 3344.

  • 2.

    Almeida CE, Duarte R, Guerra do Nascimento R, Pacheco RS, Costa J, 2002. Triatoma rubrovaria (Blanchard, 1843) (Hemiptera, Reduviidae, Triatominae) II: trophic resources and ecological observations of five populations collected in the state of Rio Grande do Sul, Brazil. Mem Inst Oswaldo Cruz 97: 11271131.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 3.

    Almeida CE, Folly-Ramos E, Agapito-Souza R, Magno-Esperança G, Pacheco RS, Costa J, 2005. Triatoma rubrovaria (Blanchart, 1843) (Hemiptera-Reduviidae-Triatominae) IV: bionomic aspects on the vector capacity of nymphs. Mem Inst Oswaldo Cruz 100: 231235.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 4.

    Alevi KCC, Rosa JA, Azeredo-Oliveira MTV, 2014. Spermatogenesis in Triatoma melanica Neiva and Lenti, 1941 (Hemiptera, Triatominae). J Vector Ecol 39: 231233.

  • 5.

    Alevi KCC, Castro NFC, Oliveira J, Rosa JA, Azeredo-Oliveira MTV, 2015. Cystic spermatogenesis in three species of the prolixus complex (Hemiptera: Triatominae). Ital J Zool (Modena) 82: 172178.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 6.

    Morielle-Souza A, Taboga SR, Azeredo-Oliveira MTV, 2010. Ultrastructural analysis of the nucleolar aspects at spermiogenesis in triatomines (Heteroptera, Triatominae). Micron 41: 791796.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 7.

    Alevi KCC, Guerra AL, Imperador CHL, Rosa JA, Azeredo-Oliveira MTV, 2016. Reproductive biology of Triatoma brasiliensis (Hemiptera, Triatominae) during the imaginal molt. Am J Trop Med Hyg 94: 689690.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 8.

    Tartarotti E, Azeredo-Oliveira MTV, 1999. Patterns of nucleolar activity during spermatogenesis of two triatomines, Panstrongylus megistus and P. herreri. Caryologia 52: 177184.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 9.

    Alevi KCC, Castro NFC, Lima ACC, Ravazi A, Morielle-Souza A, Oliveira J, Rosa JA, Azeredo-Oliveira MTV, 2014. Nucleolar persistence during spermatogenesis of the genus Rhodnius (Hemiptera, Triatominae). Cell Biol Int 38: 977980.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 10.

    Howell WM, Black DA, 1980. Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 10141015.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 11.

    Costa LC, Azeredo-Oliveira MTV, Tartarotti E, 2008. Spermatogenesis and nucleolar activity in Triatoma klugi (Triatomine, Heteroptera). Genet Mol Biol 31: 438444.

  • 12.

    Alevi KCC, Mendonça PP, Pereira NP, Rosa JA, Azeredo-Oliveira MTV, 2013. Análise das possíveis regiões organizadoras nucleolares e da atividade nucleolar em Triatoma melanocephala e T. lenti, importantes vetores da doença de Chagas. Rev Cienc Farm Básica e Apl 34: 417421.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 13.

    Bardella VB, Azeredo-Oliveira MTV, Tartarotti E, 2008. Cytogenetic analysis in the spermatogenesis of Triatoma melanosoma (Reduviidae; Heteroptera). Genet Mol Res 7: 326335.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 14.

    Severi-Aguiar GDC, Azeredo-Oliveira MTV, 2005. Cytogenetic study on three species of the genus Triatoma (Heteroptera: Reduviidae) with emphasis on nucleolar organizer regions. Caryologia 58: 293299.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 15.

    Severi-Aguiar GDC, Lourenço LB, Bicudo HEMC, Azeredo-Oliveira MTV, 2006. Meiosis aspects and nucleolar activity in Triatoma vitticeps (Triatominae, Heteroptera). Genetica 126: 141151.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 16.

    Pereira NP, Alevi KCC, Mendonça PP, Azeredo-Oliveira MTV, 2015. Spermatogenesis and nucleolar behavior in Triatoma vandae and Triatoma williami (Hemiptera, Triatominae). Genet Mol Res 14: 1214512151.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 17.

    Borgueti AO, Alevi KCC, Silistino-Souza R, Rosa JA, Azeredo-Oliveira MTV, 2015. Immunofluorescence and ultrastructural analysis of the chromatoid body during spermatogenesis of Triatoma platensis and T. rubrovaria (Hemiptera, Triatominae). Micron 74: 4446.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 18.

    Madeira FF, Lima ACC, Rosa JA, Azeredo-Oliveira MTV, Alevi KCC, 2016. Nucleolar persistence phenomenon during spermatogenesis in genus Meccus (Hemiptera, Triatominae). Genet Mol Res 15. doi:10.4238/gmr.15017427.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 19.

    Morielle A, Azeredo-Oliveira MTV, 2004. Description of the nucleolar activity and karyotype in germinative cell lines of Rhodnius domesticus (Triatominae, Heteroptera). Caryologia 57: 3137.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 20.

    Alevi KCC, Mendonça PP, Pereira NP, Rosa JA, Azeredo-Oliveira MTV, 2014. Is there post-meiotic transcriptional activity during hemipteran spermiogenesis? Invertebr Reprod Dev 58: 193198.

    • PubMed
    • Search Google Scholar
    • Export Citation
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