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CD31 Expression on CD4+ Cells: A Simple Method for Quantitation of Recent Thymus Emigrant CD4 Cells

Ramia ZakhourDepartment of Pediatrics, University of Texas Medical School, Houston, Texas.

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Dat Q. TranDepartment of Pediatrics, University of Texas Medical School, Houston, Texas.

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Gloria P. HeresiDepartment of Pediatrics, University of Texas Medical School, Houston, Texas.

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Guenet DegaffeDepartment of Pediatrics, University of Texas Medical School, Houston, Texas.

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Cynthia S. BellDepartment of Pediatrics, University of Texas Medical School, Houston, Texas.

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Elizabeth DonnachieDepartment of Pediatrics, University of Texas Medical School, Houston, Texas.

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Weihe ZhangDepartment of Pediatrics, University of Texas Medical School, Houston, Texas.

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Norma PérezDepartment of Pediatrics, University of Texas Medical School, Houston, Texas.

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Laura J. BenjaminsDepartment of Pediatrics, University of Texas Medical School, Houston, Texas.

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Gabriela Del BiancoDepartment of Pediatrics, University of Texas Medical School, Houston, Texas.

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Gilhen RodriguezDepartment of Pediatrics, University of Texas Medical School, Houston, Texas.

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James R. MurphyDepartment of Pediatrics, University of Texas Medical School, Houston, Texas.

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Measurements of CD4+CD31+ cells gave results consistent with those expected for recent thymus emigrant (RTE) CD4+ cells. The method was markedly simpler than established procedures for measurement of CD4+ RTE cells and is usable in locations with limited facilities and budgets.

Author Notes

* Address correspondence to James R. Murphy, Department of Pediatrics, Division of Infectious Diseases, University of Texas Medical School, 6431 Fannin, MSB 3.204, Houston, TX 77030. E-mail: james.r.murphy@uth.tmc.edu

Financial support: This work was funded by the State of Texas HIV Initiative.

Authors' addresses: Ramia Zakhour, Dat Q. Tran, Gloria P. Heresi, Guenet Degaffe, Cynthia S. Bell, Elizabeth Donnachie, Weihe Zhang, Norma Pérez, Laura J. Benjamins, Gabriela Del Bianco, Gilhen Rodriguez, and James R. Murphy, Department of Pediatrics, University of Texas Medical School, Houston, TX, E-mails: ramia.g.zakhour@uth.tmc.edu, dat.q.tran@uth.tmc.edu, gloria.p.heresi@uth.tmc.edu, guenet.degaffe@uth.tmc.edu, cynthia.bell@uth.tmc.edu, elizabeth.donnachie@uth.tmc.edu, weihe.zhang@uth.tmc.edu, norma.perez@uth.tmc.edu, laura.j.benjamins@uth.tmc.edu, gabriela.del.bianco@uth.tmc.edu, gilhen.rodriguez@uth.tmc.edu, and james.r.murphy@uth.tmc.edu.

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