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RefWorks
Reference Manager
BibTeX
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-
1.
Llanes A, Restrepo CM, del Vecchio G, Anguizola FJ, Lleonart R, 2015. The genome of Leishmania panamensis: insights into genomics of the L. (Viannia) subgenus. Sci Rep 5: 8550.
-
2.
Reveiz L, Maia-Elkhoury AN, Nicholls RS, Romero GA, Yadon ZE, 2013. Interventions for American cutaneous and mucocutaneous leishmaniasis: a systematic review update. PLoS One 8: e61843.
-
3.
Ministerio de Salud Pública del Ecuador (MSP), 2013. Manual de procedimientos del Subsistema alerta acción SIVE-ALERTA. Available at: https://aplicaciones.msp.gob.ec/salud/archivosdigitales/documentosDirecciones/dnn/archivos/manual_de_procedimientos_sive-alerta.pdf. Accessed January 11, 2016.
-
5.
Hartley MA, Drexler S, Ronet C, Beverley SM, Fasel N, 2014. The immunological, environmental, and phylogenetic perpetrators of metastatic leishmaniasis. Trends Parasitol 30: 412–422.
-
6.
Luna AM, Michelena MA, Fleming C, Arena G, Chiavassa AM, Rafti P, 2012. Mucocutaneous leishmaniasis with laryngeal involvement. Med Cutan Ibero Lat Am 40: 154–157.
-
7.
Kato H, Calvopiña M, Criollo H, Hashiguchi Y, 2013. First human cases of Leishmania (Viannia) naiffi infection in Ecuador and identification of its suspected vector species. Acta Trop 128: 710–713.
-
8.
Calvopina M, Armijos R, Marco J, Uezato H, Kato H, Gómez E, Korenaga M, Barroso P, Mimori T, Cooper P, Nonaka S, Hashiguchi Y, 2006. Leishmania isoenzyme polymorphisms in Ecuador: relationships with geographic distribution and clinical presentation. BMC Infect Dis 6: 136–139.
-
9.
Mouttaki T, Morales-Yuste M, Merino-Espinosa G, Chiheb S, Fellah H, Martin-Sanchez J, Riyad M, 2014. Molecular diagnosis of cutaneous leishmaniasis and identification of the causative Leishmania species in Morocco by using three PCR-based assays. Parasit Vectors 7: 420.
-
10.
Bensoussan E, Nasereddin A, Jonas F, Schnur LF, Jaffe CL, 2006. Comparison of PCR assays for diagnosis of cutaneous leishmaniasis. J Clin Microbiol 44: 1435–1439.
-
11.
Vergel C, Palacios R, Cadena H, Posso CJ, Valderrama L, Perez M, Walker J, Travi BL, Saravia NG, 2006. Evidence for Leishmania (Viannia) parasites in the skin and blood of patients before and after treatment. J Infect Dis 194: 503–511.
-
12.
Venazzi EA, Roberto AC, Barbosa-Tessmann IP, Zanzarini PD, Lonardoni MV, Silveira TG, 2007. Detection of Leishmania (Viannia) DNA in blood from patients with American cutaneous leishmaniasis. Exp Parasitol 115: 399–402.
-
13.
Ontario Public Health, 2014. Specimen Collection Guide for Cutaneous Leishmaniasis. Available at https://www.publichealthontario.ca/en/eRepository/Specimen_collection_guide_cutaneous_leishmaniasis.pdf. Accessed January 18, 2016.
-
14.
Nzelu CO, Gomez EA, Cáceres AG, Sakurai T, Martini-Robles L, Uezato H, Mimori T, Katakura K, Hashiguchi Y, Kato H, 2014. Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for Leishmania infection. Acta Trop 132: 1–6.
-
15.
Foulet F, Botterel F, Buffet P, Morizot D, Rivollet D, Deniau M, Pratlong F, Costa JM, Bretagne S, 2007. Detection and identification of Leishmania species from clinical specimens by using a real-time PCR assay and sequencing of the cytochrome b gene. J Clin Microbiol 45: 2110–2115.
-
16.
Yang B-B, Chen D-L, Chen J-P, Liao L, Hu X-S, Xu J-N, 2013. Analysis of kinetoplast cytochrome b gene of 16 Leishmania isolates from different foci of China: different species of Leishmania in China and their phylogenetic inference. Parasit Vectors 6: 32.
-
17.
Romero GA, Guerra MV, Paes MG, Macedo VO, 2001. Comparison of cutaneous leishmaniasis due to Leishmania (Viannia) braziliensis and L. (V.) guyanensis in Brazil: therapeutic response to meglumine antimoniate. Am J Trop Med Hyg 65: 456–465.
-
18.
Bañuls AL, Hide M, Prugnolle F, 2007. Leishmania and the leishmaniases: a parasite genetic update and advances in taxonomy, epidemiology and pathogenicity in humans. Adv Parasitol 64: 1–109.
-
19.
Couppié P, Clyti E, Sobesky M, Bissuel F, Del Giudice P, Sainte-Marie D, Dedet JP, Carme B, Pradinaud R, 2004. Comparative study of cutaneous leishmaniasis in human immunodeficiency virus (HIV)-infected patients and non-HIV-infected patients in French Guiana. Br J Dermatol 151: 1165–1171.
-
20.
Mandal G, Mandal S, Sharma M, Charret KS, Papadopoulou B, Bhattacharjee H, Mukhopadhyay R, 2015. Species-specific antimonial sensitivity in Leishmania is driven by post-transcriptional regulation of AQP1. PLoS Negl Trop Dis 9: e0003500.
-
21.
Neitzke-Abreu HC, Venazzi MS, Bernal MV, Reinhold-Castro KR, Vagetti F, Mota CA, Silva NR, Aristides SM, Silveira TG, Lonardoni MV, 2013. Detection of DNA from Leishmania (Viannia): accuracy of polymerase chain reaction for the diagnosis of cutaneous leishmaniasis. PLoS One 8: e62473.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 295 | 217 | 4 |
| Full Text Views | 545 | 6 | 1 |
| PDF Downloads | 126 | 12 | 4 |
Diagnostic Efficacy of Molecular Techniques for Detection and Identification of Leishmania Species in Human Whole Blood and Skin Samples from Ecuador
Erika B. Muñoz
Erika B. Muñoz
Centro de Investigación Traslacional, Universidad de Las Américas (CIT-UDLA), Quito, Ecuador.
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Stephanie Santander
Stephanie Santander
Centro de Investigación Traslacional, Universidad de Las Américas (CIT-UDLA), Quito, Ecuador.
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Patricio Rojas-Silva
Patricio Rojas-Silva
Centro de Investigación Traslacional, Universidad de Las Américas (CIT-UDLA), Quito, Ecuador.
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Paul A. Cardenas
Paul A. Cardenas
Centro de Investigación Traslacional, Universidad de Las Américas (CIT-UDLA), Quito, Ecuador.
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Marco Fornasini
Marco Fornasini
Centro de Investigación Traslacional, Universidad de Las Américas (CIT-UDLA), Quito, Ecuador.
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Sara C. Cifuentes
Sara C. Cifuentes
Centro de Investigación Traslacional, Universidad de Las Américas (CIT-UDLA), Quito, Ecuador.
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Daniela Salvador
Daniela Salvador
Instituto Ecuatoriano de Seguridad Social, Quito, Ecuador.
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Manuel E. Baldeón
Manuel E. Baldeón
Centro de Investigación Traslacional, Universidad de Las Américas (CIT-UDLA), Quito, Ecuador.
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Microscopic examination is the standard method for diagnosis of cutaneous and mucocutaneous leishmaniasis despite its low sensitivity. This study compared the diagnosis efficacy of microscopic examination versus polymerase chain reaction (PCR)–based methods and DNA sequencing using whole blood and skin lesion samples from patients with suspected leishmaniasis. The presence of Leishmania was determined by microscopy and amplification of 18S ribosomal RNA gene from blood and skin samples of 22 patients. Twenty individuals were positive for leishmaniasis. Microscopic analysis identified 85%, whereas PCR identified 100% of positive cases from skin and 90% from blood. Cytochrome b gene (cyt-b) amplification and sequencing identified Leishmania guyanensis, Leishmania shawi, and Leishmania naiffi from skin and blood samples. This study demonstrated the usefulness of whole blood and molecular techniques for the diagnosis and species identification of leishmaniasis.
Author Notes
Authors' addresses: Erika B. Muñoz, Stephanie Santander, Patricio Rojas-Silva, Paul A. Cardenas, Marco Fornasini, Sara C. Cifuentes, and Manuel E. Baldeón, Centro de Investigación Traslacional, Universidad de Las Américas, Quito, Ecuador, E-mails: erika.munoz@udla.edu.ec, chefasan@hotmail.com, patricio.rojas@udla.edu.ec, paul.cardenas@udla.edu.ec, marco.fornasini@udla.edu.ec, s.cifuentes@udlanet.ec, and manuel.baldeon@udla.edu.ec. Daniela Salvador, Emergencia, Instituto Ecuatoriano de Seguridad Social, Quito, Ecuador, E-mail: dani_salvador88@hotmail.com.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Llanes A, Restrepo CM, del Vecchio G, Anguizola FJ, Lleonart R, 2015. The genome of Leishmania panamensis: insights into genomics of the L. (Viannia) subgenus. Sci Rep 5: 8550.
-
2.
Reveiz L, Maia-Elkhoury AN, Nicholls RS, Romero GA, Yadon ZE, 2013. Interventions for American cutaneous and mucocutaneous leishmaniasis: a systematic review update. PLoS One 8: e61843.
-
3.
Ministerio de Salud Pública del Ecuador (MSP), 2013. Manual de procedimientos del Subsistema alerta acción SIVE-ALERTA. Available at: https://aplicaciones.msp.gob.ec/salud/archivosdigitales/documentosDirecciones/dnn/archivos/manual_de_procedimientos_sive-alerta.pdf. Accessed January 11, 2016.
-
5.
Hartley MA, Drexler S, Ronet C, Beverley SM, Fasel N, 2014. The immunological, environmental, and phylogenetic perpetrators of metastatic leishmaniasis. Trends Parasitol 30: 412–422.
-
6.
Luna AM, Michelena MA, Fleming C, Arena G, Chiavassa AM, Rafti P, 2012. Mucocutaneous leishmaniasis with laryngeal involvement. Med Cutan Ibero Lat Am 40: 154–157.
-
7.
Kato H, Calvopiña M, Criollo H, Hashiguchi Y, 2013. First human cases of Leishmania (Viannia) naiffi infection in Ecuador and identification of its suspected vector species. Acta Trop 128: 710–713.
-
8.
Calvopina M, Armijos R, Marco J, Uezato H, Kato H, Gómez E, Korenaga M, Barroso P, Mimori T, Cooper P, Nonaka S, Hashiguchi Y, 2006. Leishmania isoenzyme polymorphisms in Ecuador: relationships with geographic distribution and clinical presentation. BMC Infect Dis 6: 136–139.
-
9.
Mouttaki T, Morales-Yuste M, Merino-Espinosa G, Chiheb S, Fellah H, Martin-Sanchez J, Riyad M, 2014. Molecular diagnosis of cutaneous leishmaniasis and identification of the causative Leishmania species in Morocco by using three PCR-based assays. Parasit Vectors 7: 420.
-
10.
Bensoussan E, Nasereddin A, Jonas F, Schnur LF, Jaffe CL, 2006. Comparison of PCR assays for diagnosis of cutaneous leishmaniasis. J Clin Microbiol 44: 1435–1439.
-
11.
Vergel C, Palacios R, Cadena H, Posso CJ, Valderrama L, Perez M, Walker J, Travi BL, Saravia NG, 2006. Evidence for Leishmania (Viannia) parasites in the skin and blood of patients before and after treatment. J Infect Dis 194: 503–511.
-
12.
Venazzi EA, Roberto AC, Barbosa-Tessmann IP, Zanzarini PD, Lonardoni MV, Silveira TG, 2007. Detection of Leishmania (Viannia) DNA in blood from patients with American cutaneous leishmaniasis. Exp Parasitol 115: 399–402.
-
13.
Ontario Public Health, 2014. Specimen Collection Guide for Cutaneous Leishmaniasis. Available at https://www.publichealthontario.ca/en/eRepository/Specimen_collection_guide_cutaneous_leishmaniasis.pdf. Accessed January 18, 2016.
-
14.
Nzelu CO, Gomez EA, Cáceres AG, Sakurai T, Martini-Robles L, Uezato H, Mimori T, Katakura K, Hashiguchi Y, Kato H, 2014. Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for Leishmania infection. Acta Trop 132: 1–6.
-
15.
Foulet F, Botterel F, Buffet P, Morizot D, Rivollet D, Deniau M, Pratlong F, Costa JM, Bretagne S, 2007. Detection and identification of Leishmania species from clinical specimens by using a real-time PCR assay and sequencing of the cytochrome b gene. J Clin Microbiol 45: 2110–2115.
-
16.
Yang B-B, Chen D-L, Chen J-P, Liao L, Hu X-S, Xu J-N, 2013. Analysis of kinetoplast cytochrome b gene of 16 Leishmania isolates from different foci of China: different species of Leishmania in China and their phylogenetic inference. Parasit Vectors 6: 32.
-
17.
Romero GA, Guerra MV, Paes MG, Macedo VO, 2001. Comparison of cutaneous leishmaniasis due to Leishmania (Viannia) braziliensis and L. (V.) guyanensis in Brazil: therapeutic response to meglumine antimoniate. Am J Trop Med Hyg 65: 456–465.
-
18.
Bañuls AL, Hide M, Prugnolle F, 2007. Leishmania and the leishmaniases: a parasite genetic update and advances in taxonomy, epidemiology and pathogenicity in humans. Adv Parasitol 64: 1–109.
-
19.
Couppié P, Clyti E, Sobesky M, Bissuel F, Del Giudice P, Sainte-Marie D, Dedet JP, Carme B, Pradinaud R, 2004. Comparative study of cutaneous leishmaniasis in human immunodeficiency virus (HIV)-infected patients and non-HIV-infected patients in French Guiana. Br J Dermatol 151: 1165–1171.
-
20.
Mandal G, Mandal S, Sharma M, Charret KS, Papadopoulou B, Bhattacharjee H, Mukhopadhyay R, 2015. Species-specific antimonial sensitivity in Leishmania is driven by post-transcriptional regulation of AQP1. PLoS Negl Trop Dis 9: e0003500.
-
21.
Neitzke-Abreu HC, Venazzi MS, Bernal MV, Reinhold-Castro KR, Vagetti F, Mota CA, Silva NR, Aristides SM, Silveira TG, Lonardoni MV, 2013. Detection of DNA from Leishmania (Viannia): accuracy of polymerase chain reaction for the diagnosis of cutaneous leishmaniasis. PLoS One 8: e62473.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 295 | 217 | 4 |
| Full Text Views | 545 | 6 | 1 |
| PDF Downloads | 126 | 12 | 4 |