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-
1.
Walther M, Muller R, 2003. Diagnosis of human filariases (except onchocerciasis). Adv Parasitol 53: 149–193.
-
2.
Rosenblatt JE, 2009. Laboratory diagnosis of infections due to blood and tissue parasites. Clin Infect Dis 49: 1103–1108.
-
3.
Beaver PC, Neel JV, Orihel TC, 1976. Dipetalonema perstans and Mansonella ozzardi in Indians of southern Venezuela. Am J Trop Med Hyg 25: 263–265.
-
4.
Gomez J, Guerrero R, 2000. Environmental factors and the distribution of mansonelliases in southern Venezuela. Parasite 7: 71–76.
-
5.
Marinkelle CJ, German E, 1970. Mansonelliasis in the Comisaria del Vaupes of Colombia. Trop Geogr Med 22: 101–111.
-
6.
Kozek WJ, Palma G, Henao A, Garcia H, Hoyos M, 1983. Filariasis in Colombia: prevalence and distribution of Mansonella ozzardi and Mansonella (= Dipetalonema) perstans infections in the Comisaria del Guainia. Am J Trop Med Hyg 32: 379–384.
-
7.
Orihel TC, 1967. Infections with Dipetalonema perstans and Mansonella ozzardi in the aboriginal Indians of Guyana. Am J Trop Med Hyg 16: 628–635.
-
8.
Adami YL, Moraes MA, Lanfredi RM, Maia-Herzog M, 2008. An atypical microfilaria in blood samples from inhabitants of Brazilian Amazon. Parasitol Res 104: 95–99.
-
9.
Marcos LA, Arrospide N, Recuenco S, Cabezas C, Weil GJ, Fischer PU, 2012. Genetic characterization of atypical Mansonella (Mansonella) ozzardi microfilariae in human blood samples from northeastern Peru. Am J Trop Med Hyg 87: 491–494.
-
10.
Godoy GA, Orihel TC, Volcan GS, 1980. Microfilaria bolivarensis: a new species of filaria from man in Venezuela. Am J Trop Med Hyg 29: 545–547.
-
11.
Eberhard ML, Lammie PJ, 1991. Laboratory diagnosis of filariasis. Clin Lab Med 11: 977–1010.
-
12.
Tang TH, Lopez-Velez R, Lanza M, Shelley AJ, Rubio JM, Luz SL, 2010. Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon region. Mem Inst Oswaldo Cruz 105: 823–828.
-
13.
Hojas RM, Post RJ, 2000. Regional genetic variation in the major sperm protein genes of Onchocerca volvulus and Mansonella ozzardi (Nematoda: Filarioidea). Int J Parasitol 30: 1459–1465.
-
14.
Thompson JD, Higgins DG, Gibson TJ, 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673–4680.
-
15.
Van de Peer Y, De Wachter R, 1994. TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10: 569–570.
-
16.
Luz SL, Crainey JL, Shelley AJ, Rubio M, 2014. Outstanding insecurities concerning the use of an Ov16-based ELISA in the Amazonia onchocerciasis focus. Mem Inst Oswaldo Cruz 109: 506–508.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 825 | 643 | 194 |
| Full Text Views | 539 | 12 | 0 |
| PDF Downloads | 234 | 10 | 2 |
Atypical Mansonella ozzardi Microfilariae from an Endemic Area of Brazilian Amazonia
Thuy-Huong Ta-Tang
Thuy-Huong Ta-Tang
Malaria and Emerging Parasitic Diseases Laboratory, Parasitology Department, National Microbiology Center, Instituto de Salud Carlos III, Madrid, Spain.
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Sergio L. B. Luz
Sergio L. B. Luz
Instituto Leônidas e Maria Deane, Fundação Oswaldo Cruz (FIOCRUZ) Amazônia, Manaus, Brazil.
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Francisco J. Merino
Francisco J. Merino
Severo Ochoa University Hospital, Madrid, Spain.
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Isabel de Fuentes
Isabel de Fuentes
Malaria and Emerging Parasitic Diseases Laboratory, Parasitology Department, National Microbiology Center, Instituto de Salud Carlos III, Madrid, Spain.
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Rogelio López-Vélez
Rogelio López-Vélez
Tropical Medicine and Clinical Parasitology Unit, Infectious Diseases Department, Hospital Ramón y Cajal, Madrid, Spain.
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Tatiana A. P. Almeida
Tatiana A. P. Almeida
Instituto Leônidas e Maria Deane, Fundação Oswaldo Cruz (FIOCRUZ) Amazônia, Manaus, Brazil.
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Marta Lanza
Marta Lanza
Malaria and Emerging Parasitic Diseases Laboratory, Parasitology Department, National Microbiology Center, Instituto de Salud Carlos III, Madrid, Spain.
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Cláudia M. M. Abrahim
Cláudia M. M. Abrahim
Fundação de Hematologia e Hemoterapia do Amazonas (HEMOAM), Manaus, Brazil.
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José M. Rubio
José M. Rubio
Malaria and Emerging Parasitic Diseases Laboratory, Parasitology Department, National Microbiology Center, Instituto de Salud Carlos III, Madrid, Spain.
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Mansonellosis is endemic in several regions of Africa, the Caribbean, and Latin America. Mansonella ozzardi and Mansonella perstans have been reported in Latin America, including the Amazon region. A morphological and molecular microfilariae study was performed in Pauini (Brazil). Blood samples were collected from 40 individuals, and were analyzed by Giemsa-stained blood film and by two different nested polymerase chain reactions which detect internal transcribed spacer-1 and the major sperm protein gene. By microscopy, 14 of 40 were positive: 11 as M. ozzardi and three as M. perstans–like infections. Both molecular methods detected 19 positive cases as M. ozzardi, including those 14 individuals detected by microscopy, without detectable genetic differences among any of the 19 positive samples. Molecular techniques showed an improvement of mansonellosis diagnosis and may become an effective tool to evaluate the present status of M. ozzardi and M. perstans in Latin America.
Author Notes
Authors' addresses: Thuy-Huong Ta-Tang, Isabel de Fuentes, Marta Lanza, and José M. Rubio, Malaria and Emerging Parasitic Diseases Laboratory, Parasitology Department, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Madrid, Spain, E-mails: thuyhuong.tatang@gmail.com, ifuentes@isciii.es, mlanza@isciii.es, and jmrubio@isciii.es. Sergio L. B. Luz and Tatiana A. P. Almeida, Parasitology, Instituto Leônidas e Maria Deane, Fundação Oswaldo Cruz (FIOCRUZ), Manaus, Brazil, E-mails: sergioluz@amazonia.fiocruz.br and tatiana_almeida@amazonia.fiocruz.br. Francisco J. Merino, Microbiology and Parasitology, Severo Ochoa University Hospital, Madrid, Spain, E-mail: fmerino.hsvo@salud.madrid.org. Rogelio López-Vélez, Tropical Medicine and Clinical Parasitology Unit, Infectious Diseases Department, Hospital Ramón y Cajal, Madrid, Spain, E-mail: rogelio.lopezvelez@salud.madrid.org. Cláudia M. M. Abrahim, Fundação de Hematologia e Hemoterapia do Amazonas (HEMOAM), Manaus, Brazil, E-mail: cmmabrahim@hemoam.am.gov.br.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Walther M, Muller R, 2003. Diagnosis of human filariases (except onchocerciasis). Adv Parasitol 53: 149–193.
-
2.
Rosenblatt JE, 2009. Laboratory diagnosis of infections due to blood and tissue parasites. Clin Infect Dis 49: 1103–1108.
-
3.
Beaver PC, Neel JV, Orihel TC, 1976. Dipetalonema perstans and Mansonella ozzardi in Indians of southern Venezuela. Am J Trop Med Hyg 25: 263–265.
-
4.
Gomez J, Guerrero R, 2000. Environmental factors and the distribution of mansonelliases in southern Venezuela. Parasite 7: 71–76.
-
5.
Marinkelle CJ, German E, 1970. Mansonelliasis in the Comisaria del Vaupes of Colombia. Trop Geogr Med 22: 101–111.
-
6.
Kozek WJ, Palma G, Henao A, Garcia H, Hoyos M, 1983. Filariasis in Colombia: prevalence and distribution of Mansonella ozzardi and Mansonella (= Dipetalonema) perstans infections in the Comisaria del Guainia. Am J Trop Med Hyg 32: 379–384.
-
7.
Orihel TC, 1967. Infections with Dipetalonema perstans and Mansonella ozzardi in the aboriginal Indians of Guyana. Am J Trop Med Hyg 16: 628–635.
-
8.
Adami YL, Moraes MA, Lanfredi RM, Maia-Herzog M, 2008. An atypical microfilaria in blood samples from inhabitants of Brazilian Amazon. Parasitol Res 104: 95–99.
-
9.
Marcos LA, Arrospide N, Recuenco S, Cabezas C, Weil GJ, Fischer PU, 2012. Genetic characterization of atypical Mansonella (Mansonella) ozzardi microfilariae in human blood samples from northeastern Peru. Am J Trop Med Hyg 87: 491–494.
-
10.
Godoy GA, Orihel TC, Volcan GS, 1980. Microfilaria bolivarensis: a new species of filaria from man in Venezuela. Am J Trop Med Hyg 29: 545–547.
-
11.
Eberhard ML, Lammie PJ, 1991. Laboratory diagnosis of filariasis. Clin Lab Med 11: 977–1010.
-
12.
Tang TH, Lopez-Velez R, Lanza M, Shelley AJ, Rubio JM, Luz SL, 2010. Nested PCR to detect and distinguish the sympatric filarial species Onchocerca volvulus, Mansonella ozzardi and Mansonella perstans in the Amazon region. Mem Inst Oswaldo Cruz 105: 823–828.
-
13.
Hojas RM, Post RJ, 2000. Regional genetic variation in the major sperm protein genes of Onchocerca volvulus and Mansonella ozzardi (Nematoda: Filarioidea). Int J Parasitol 30: 1459–1465.
-
14.
Thompson JD, Higgins DG, Gibson TJ, 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673–4680.
-
15.
Van de Peer Y, De Wachter R, 1994. TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10: 569–570.
-
16.
Luz SL, Crainey JL, Shelley AJ, Rubio M, 2014. Outstanding insecurities concerning the use of an Ov16-based ELISA in the Amazonia onchocerciasis focus. Mem Inst Oswaldo Cruz 109: 506–508.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 825 | 643 | 194 |
| Full Text Views | 539 | 12 | 0 |
| PDF Downloads | 234 | 10 | 2 |