D2 Region of the 28S RNA Gene: A Too-Conserved Fragment for Inferences on Phylogeny of South American Triatomines

Ana Letícia Guerra Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, São José do Rio Preto, Sao Paulo, Brazil.

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Kaio Cesar Chaboli Alevi Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, São José do Rio Preto, Sao Paulo, Brazil.

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Cecília Artico Banho Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, São José do Rio Preto, Sao Paulo, Brazil.

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Jader de Oliveira Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista, Araraquara, Sao Paulo, Brazil.

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João Aristeu da Rosa Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista, Araraquara, Sao Paulo, Brazil.

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Maria Tercília Vilela de Azeredo-Oliveira Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, São José do Rio Preto, Sao Paulo, Brazil.

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The brasiliensis complex is composed of five triatomine species, and different approaches suggest that Triatoma lenti and Triatoma petrochiae may be the new members. Therefore, this study sought to analyze the phylogenetic relationships within this complex by means of the D2 region of the 28S RNA gene, and to analyze the degree of polymorphism and phylogenetic significance of this gene for South American triatomines. Phylogenetic analysis by using sequence fragments of the D2 domain did not allow to perform phylogenetic inferences on species within the brasiliensis complex, because the gene alignment composed of a matrix with 37 specimens exhibited only two variable sites along the 567 base pairs used. Furthermore, if all South American species are included, only four variable sites were detected, reflecting the high degree of gene conservation. Therefore, we do not recommend the use of this gene for phylogenetic reconstruction for this group of Chagas disease vectors.

Author Notes

* Address correspondence to Kaio Cesar Chaboli Alevi, Instituto de Biociências, Letras e Ciências Exatas, (IBILCE–UNESP), Rua Cristovão Colombo, 2265, Jardim Nazareth, CEP 15054-000, São José do Rio Preto, Sao Paulo, Brazil. E-mail: kaiochaboli@hotmail.com

Financial support: The research was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

Authors' addresses: Ana Letícia Guerra, Kaio Cesar Chaboli Alevi, Cecília Artico Banho, and Maria Tercília Vilela de Azeredo-Oliveira, Departamento de Biologia, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista (IBILCE–UNESP), São José do Rio Preto, Sao Paulo, Brazil, E-mails: analebio@yahoo.com.br, kaiochaboli@hotmail.com, ce_artico@hotmail.com, and tercilia@ibilce.unesp.br. Jader de Oliveira and João Aristeu da Rosa, Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, Universidade Estadual Paulista (UNESP), Araraquara, São Paulo, Brazil, E-mails: jdr.oliveira@hotmail.com and joaoaristeu@gmail.com.

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