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-
1.
Anderson TJ, Haubold B, Williams JT, Estrada-Franco JG, Richardson L, Mollinedo R, Bockarie M, Mokili J, Mharakurwa S, French N, Whitworth J, Velez ID, Brockman AH, Nosten F, Ferreira MU, Day KP, 2000. Microsatellite markers reveal a spectrum of population structures in the malaria parasite Plasmodium falciparum. Mol Biol Evol 17: 1467–1482.
-
2.
Bogreau H, Renaud F, Bouchiba H, Durand P, Assi SB, Henry MC, Garnotel E, Pradines B, Fusai T, Wade B, Adehossi E, Parola P, Kamil MA, Puijalon O, Rogier C, 2006. Genetic diversity and structure of African Plasmodium falciparum populations in urban and rural areas. Am J Trop Med Hyg 74: 953–959.
-
3.
Mobegi VA, Loua KM, Ahouidi AD, Satoguina J, Nwakanma DC, Amambua-Ngwa A, Conway DJ, 2012. Population genetic structure of Plasmodium falciparum across a region of diverse endemicity in west Africa. Malar J 11: 223.
-
4.
Oyebola MK, Idowu ET, Nyang H, Olukosi YA, Otubanjo OA, Nwakanma DC, Awolola ST, Amambua-Ngwa A, 2014. Microsatellite markers reveal low levels of population sub-structuring of Plasmodium falciparum in southwestern Nigeria. Malar J 13: 493.
-
5.
Khaireh BA, Assefa A, Guessod HH, Basco LK, Khaireh MA, Pascual A, Briolant S, Bouh SM, Farah IH, Ali HM, Abdi AI, Aden MO, Abdillahi Z, Ayeh SN, Darar HY, Koeck JL, Rogier C, Pradines B, Bogreau H, 2013. Population genetics analysis during the elimination process of Plasmodium falciparum in Djibouti. Malar J 12: 201.
-
6.
World Health Organization, 2014. World Malaria Report 2014. WHO Global Malaria Programme. Available at: http://www.who.int/malaria/publications/world_malaria_report_2014/wmr-2014-no-profiles.pdf. Accessed October 21, 2015.
-
7.
Veron V, Carme B, 2006. Short report: recovery and use of Plasmodium DNA from malaria rapid diagnostic tests. Am J Trop Med Hyg 74: 941–943.
-
8.
Morris U, Aydin-Schmidt B, Shakely D, Mårtensson A, Jörnhagen L, Ali AS, Msellem MI, Petzold M, Gil JP, Ferreira PE, Björkman A, 2013. Rapid diagnostic tests for molecular surveillance of Plasmodium falciparum malaria—assessment of DNA extraction methods and field applicability. Malar J 12: 106.
-
9.
Cnops L, Boderie M, Gillet P, Van Esbroeck M, Jacobs J, 2011. Rapid diagnostic tests as a source of DNA for Plasmodium species-specific real-time PCR. Malar J 10: 67.
-
10.
Ishengoma DS, Lwitiho S, Madebe RA, Nyagonde N, Persson O, Vestergaard LS, Bygbjerg IC, Lemnge MM, Alifrangis M, 2011. Using rapid diagnostic tests as source of malaria parasite DNA for molecular analyses in the era of declining malaria prevalence. Malar J 10: 6.
-
11.
Boom R, Sol CJ, Salimans M, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J, 1990. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 28: 495–503.
-
12.
Rougemont M, Van Saanen M, Sahli R, Hinrikson HP, Bille J, Jaton K, 2004. Detection of four Plasmodium species in blood from humans by 18S rRNA gene subunit-based and species-specific real-time PCR assays. J Clin Microbiol 42: 5636–5643.
-
13.
Mercereau-Puijalon O, Barale J-C, Bischoff E, 2002. Three multigene families in Plasmodium parasites: facts and questions. Int J Parasitol 32: 1323–1344.
-
14.
Iqbal J, Siddique A, Jameel M, Hira PR, 2004. Persistent histidine-rich protein 2, parasite lactate dehydrogenase, and panmalarial antigen reactivity after clearance of Plasmodium falciparum monoinfection. J Clin Microbiol 42: 4237–4241.
-
15.
Bousema T, Okell L, Felger I, Drakeley C, 2014. Asymptomatic malaria infections: detectability, transmissibility and public health relevance. Nat Rev Microbiol 12: 833–840.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 701 | 566 | 28 |
| Full Text Views | 350 | 3 | 0 |
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Analyzing Deoxyribose Nucleic Acid from Malaria Rapid Diagnostic Tests to Study Plasmodium falciparum Genetic Diversity in Mali
Cécile Nabet
Cécile Nabet
UMR MD3 Infections Parasitaires Transmission Pharmacologie et Thérapeutique (IP-TPT), Aix-Marseille University, Marseilles, France; Parasitology Laboratory, Timone Hospital, Marseilles, France; Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, UMI 3189, Faculty of Medicine and Dentistry, University of Sciences, Techniques and Technology, Bamako, Mali; University of Pavia, Pavia, Italy
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Safiatou Doumbo
Safiatou Doumbo
UMR MD3 Infections Parasitaires Transmission Pharmacologie et Thérapeutique (IP-TPT), Aix-Marseille University, Marseilles, France; Parasitology Laboratory, Timone Hospital, Marseilles, France; Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, UMI 3189, Faculty of Medicine and Dentistry, University of Sciences, Techniques and Technology, Bamako, Mali; University of Pavia, Pavia, Italy
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Fakhri Jeddi
Fakhri Jeddi
UMR MD3 Infections Parasitaires Transmission Pharmacologie et Thérapeutique (IP-TPT), Aix-Marseille University, Marseilles, France; Parasitology Laboratory, Timone Hospital, Marseilles, France; Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, UMI 3189, Faculty of Medicine and Dentistry, University of Sciences, Techniques and Technology, Bamako, Mali; University of Pavia, Pavia, Italy
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Issaka Sagara
Issaka Sagara
UMR MD3 Infections Parasitaires Transmission Pharmacologie et Thérapeutique (IP-TPT), Aix-Marseille University, Marseilles, France; Parasitology Laboratory, Timone Hospital, Marseilles, France; Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, UMI 3189, Faculty of Medicine and Dentistry, University of Sciences, Techniques and Technology, Bamako, Mali; University of Pavia, Pavia, Italy
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Tommaso Manciulli
Tommaso Manciulli
UMR MD3 Infections Parasitaires Transmission Pharmacologie et Thérapeutique (IP-TPT), Aix-Marseille University, Marseilles, France; Parasitology Laboratory, Timone Hospital, Marseilles, France; Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, UMI 3189, Faculty of Medicine and Dentistry, University of Sciences, Techniques and Technology, Bamako, Mali; University of Pavia, Pavia, Italy
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Amadou Tapily
Amadou Tapily
UMR MD3 Infections Parasitaires Transmission Pharmacologie et Thérapeutique (IP-TPT), Aix-Marseille University, Marseilles, France; Parasitology Laboratory, Timone Hospital, Marseilles, France; Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, UMI 3189, Faculty of Medicine and Dentistry, University of Sciences, Techniques and Technology, Bamako, Mali; University of Pavia, Pavia, Italy
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Coralie L'Ollivier
Coralie L'Ollivier
UMR MD3 Infections Parasitaires Transmission Pharmacologie et Thérapeutique (IP-TPT), Aix-Marseille University, Marseilles, France; Parasitology Laboratory, Timone Hospital, Marseilles, France; Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, UMI 3189, Faculty of Medicine and Dentistry, University of Sciences, Techniques and Technology, Bamako, Mali; University of Pavia, Pavia, Italy
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Abdoulaye Djimde
Abdoulaye Djimde
UMR MD3 Infections Parasitaires Transmission Pharmacologie et Thérapeutique (IP-TPT), Aix-Marseille University, Marseilles, France; Parasitology Laboratory, Timone Hospital, Marseilles, France; Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, UMI 3189, Faculty of Medicine and Dentistry, University of Sciences, Techniques and Technology, Bamako, Mali; University of Pavia, Pavia, Italy
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Ogobara K. Doumbo
Ogobara K. Doumbo
UMR MD3 Infections Parasitaires Transmission Pharmacologie et Thérapeutique (IP-TPT), Aix-Marseille University, Marseilles, France; Parasitology Laboratory, Timone Hospital, Marseilles, France; Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, UMI 3189, Faculty of Medicine and Dentistry, University of Sciences, Techniques and Technology, Bamako, Mali; University of Pavia, Pavia, Italy
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Renaud Piarroux
Renaud Piarroux
UMR MD3 Infections Parasitaires Transmission Pharmacologie et Thérapeutique (IP-TPT), Aix-Marseille University, Marseilles, France; Parasitology Laboratory, Timone Hospital, Marseilles, France; Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, UMI 3189, Faculty of Medicine and Dentistry, University of Sciences, Techniques and Technology, Bamako, Mali; University of Pavia, Pavia, Italy
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We evaluated the use of positive malaria rapid diagnostic tests (mRDTs) to determine genetic diversity of Plasmodium falciparum in Mali. Genetic diversity was assessed via multiple loci variable number of tandem repeats analysis (MLVA). We performed DNA extraction from 104 positive and 30 negative used mRDTs that had been stored at ambient temperature for up to 14 months. Extracted DNA was analyzed via quantitative polymerase chain reaction (qPCR), and MLVA genotyping was then assessed on positive qPCR samples. Eighty-three of the positive mRDTs (83/104, 79.8%) and none of the negative mRDTs were confirmed P. falciparum positive via qPCR. We achieved complete genotyping of 90.4% (75/83) of the qPCR-positive samples. Genotyping revealed high genetic diversity among P. falciparum populations in Mali and an absence of population clustering. We show that mRDTs are useful to monitor P. falciparum genetic diversity and thereby can provide essential data to guide malaria control programs.
Author Notes
Financial support: Sample collection in Mali was supported by EDTCP1-WANETAM and WANECAM grants. Sample genotyping was supported by the Parasitology and Mycology Laboratory, La Timone Hospital, Marseilles, France.
Authors' addresses: Cécile Nabet, Coralie L'Ollivier, and Renaud Piarroux, UMR MD3 IP-TPT, Aix Marseille University, Marseille, France, and Parasitology and Mycology Laboratory, Assistance Publique des Hôpitaux de Marseille (APHM), Marseille, France, E-mails: cecilenabet7@gmail.com, coralie.lollivier@ap-hm.fr, and renaud.piarroux@ap-hm.fr. Safiatou Doumbo, Issaka Sagara, Amadou Tapily, Abdoulaye Djimde, and Ogobara K. Doumbo, Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, Faculty of Medicine and Dentistry, University of Sciences, Techniques and Technologies, Bamako, Mali, E-mails: sdoumbo@icermali.org, isagara@icermali.org, atapily@icermali.org, adjimde@icermali.org, and okd@icermali.org. Fakhri Jeddi, Parasitology and Mycology Laboratory, APHM, Marseille, France, E-mail: fakhri.jeddi@ap-hm.fr. Tommaso Manciulli, UMR MD3 IP-TPT, Aix-Marseille University, Marseille, France, and University of Pavia, Faculty of Medicine and Surgery, Pavia, Italy, E-mail: tommaso.manciulli01@universitadipavia.it.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Anderson TJ, Haubold B, Williams JT, Estrada-Franco JG, Richardson L, Mollinedo R, Bockarie M, Mokili J, Mharakurwa S, French N, Whitworth J, Velez ID, Brockman AH, Nosten F, Ferreira MU, Day KP, 2000. Microsatellite markers reveal a spectrum of population structures in the malaria parasite Plasmodium falciparum. Mol Biol Evol 17: 1467–1482.
-
2.
Bogreau H, Renaud F, Bouchiba H, Durand P, Assi SB, Henry MC, Garnotel E, Pradines B, Fusai T, Wade B, Adehossi E, Parola P, Kamil MA, Puijalon O, Rogier C, 2006. Genetic diversity and structure of African Plasmodium falciparum populations in urban and rural areas. Am J Trop Med Hyg 74: 953–959.
-
3.
Mobegi VA, Loua KM, Ahouidi AD, Satoguina J, Nwakanma DC, Amambua-Ngwa A, Conway DJ, 2012. Population genetic structure of Plasmodium falciparum across a region of diverse endemicity in west Africa. Malar J 11: 223.
-
4.
Oyebola MK, Idowu ET, Nyang H, Olukosi YA, Otubanjo OA, Nwakanma DC, Awolola ST, Amambua-Ngwa A, 2014. Microsatellite markers reveal low levels of population sub-structuring of Plasmodium falciparum in southwestern Nigeria. Malar J 13: 493.
-
5.
Khaireh BA, Assefa A, Guessod HH, Basco LK, Khaireh MA, Pascual A, Briolant S, Bouh SM, Farah IH, Ali HM, Abdi AI, Aden MO, Abdillahi Z, Ayeh SN, Darar HY, Koeck JL, Rogier C, Pradines B, Bogreau H, 2013. Population genetics analysis during the elimination process of Plasmodium falciparum in Djibouti. Malar J 12: 201.
-
6.
World Health Organization, 2014. World Malaria Report 2014. WHO Global Malaria Programme. Available at: http://www.who.int/malaria/publications/world_malaria_report_2014/wmr-2014-no-profiles.pdf. Accessed October 21, 2015.
-
7.
Veron V, Carme B, 2006. Short report: recovery and use of Plasmodium DNA from malaria rapid diagnostic tests. Am J Trop Med Hyg 74: 941–943.
-
8.
Morris U, Aydin-Schmidt B, Shakely D, Mårtensson A, Jörnhagen L, Ali AS, Msellem MI, Petzold M, Gil JP, Ferreira PE, Björkman A, 2013. Rapid diagnostic tests for molecular surveillance of Plasmodium falciparum malaria—assessment of DNA extraction methods and field applicability. Malar J 12: 106.
-
9.
Cnops L, Boderie M, Gillet P, Van Esbroeck M, Jacobs J, 2011. Rapid diagnostic tests as a source of DNA for Plasmodium species-specific real-time PCR. Malar J 10: 67.
-
10.
Ishengoma DS, Lwitiho S, Madebe RA, Nyagonde N, Persson O, Vestergaard LS, Bygbjerg IC, Lemnge MM, Alifrangis M, 2011. Using rapid diagnostic tests as source of malaria parasite DNA for molecular analyses in the era of declining malaria prevalence. Malar J 10: 6.
-
11.
Boom R, Sol CJ, Salimans M, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J, 1990. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 28: 495–503.
-
12.
Rougemont M, Van Saanen M, Sahli R, Hinrikson HP, Bille J, Jaton K, 2004. Detection of four Plasmodium species in blood from humans by 18S rRNA gene subunit-based and species-specific real-time PCR assays. J Clin Microbiol 42: 5636–5643.
-
13.
Mercereau-Puijalon O, Barale J-C, Bischoff E, 2002. Three multigene families in Plasmodium parasites: facts and questions. Int J Parasitol 32: 1323–1344.
-
14.
Iqbal J, Siddique A, Jameel M, Hira PR, 2004. Persistent histidine-rich protein 2, parasite lactate dehydrogenase, and panmalarial antigen reactivity after clearance of Plasmodium falciparum monoinfection. J Clin Microbiol 42: 4237–4241.
-
15.
Bousema T, Okell L, Felger I, Drakeley C, 2014. Asymptomatic malaria infections: detectability, transmissibility and public health relevance. Nat Rev Microbiol 12: 833–840.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 701 | 566 | 28 |
| Full Text Views | 350 | 3 | 0 |
| PDF Downloads | 71 | 4 | 0 |