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Analyzing Deoxyribose Nucleic Acid from Malaria Rapid Diagnostic Tests to Study Plasmodium falciparum Genetic Diversity in Mali

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  • UMR MD3 Infections Parasitaires Transmission Pharmacologie et Thérapeutique (IP-TPT), Aix-Marseille University, Marseilles, France; Parasitology Laboratory, Timone Hospital, Marseilles, France; Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, UMI 3189, Faculty of Medicine and Dentistry, University of Sciences, Techniques and Technology, Bamako, Mali; University of Pavia, Pavia, Italy

We evaluated the use of positive malaria rapid diagnostic tests (mRDTs) to determine genetic diversity of Plasmodium falciparum in Mali. Genetic diversity was assessed via multiple loci variable number of tandem repeats analysis (MLVA). We performed DNA extraction from 104 positive and 30 negative used mRDTs that had been stored at ambient temperature for up to 14 months. Extracted DNA was analyzed via quantitative polymerase chain reaction (qPCR), and MLVA genotyping was then assessed on positive qPCR samples. Eighty-three of the positive mRDTs (83/104, 79.8%) and none of the negative mRDTs were confirmed P. falciparum positive via qPCR. We achieved complete genotyping of 90.4% (75/83) of the qPCR-positive samples. Genotyping revealed high genetic diversity among P. falciparum populations in Mali and an absence of population clustering. We show that mRDTs are useful to monitor P. falciparum genetic diversity and thereby can provide essential data to guide malaria control programs.

Author Notes

* Address correspondence to Renaud Piarroux, Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire de La Timone, 264 rue Saint Pierre, 13385 Marseilles Cedex 5, France. E-mail: renaud.piarroux@ap-hm.fr

Financial support: Sample collection in Mali was supported by EDTCP1-WANETAM and WANECAM grants. Sample genotyping was supported by the Parasitology and Mycology Laboratory, La Timone Hospital, Marseilles, France.

Authors' addresses: Cécile Nabet, Coralie L'Ollivier, and Renaud Piarroux, UMR MD3 IP-TPT, Aix Marseille University, Marseille, France, and Parasitology and Mycology Laboratory, Assistance Publique des Hôpitaux de Marseille (APHM), Marseille, France, E-mails: cecilenabet7@gmail.com, coralie.lollivier@ap-hm.fr, and renaud.piarroux@ap-hm.fr. Safiatou Doumbo, Issaka Sagara, Amadou Tapily, Abdoulaye Djimde, and Ogobara K. Doumbo, Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, Faculty of Medicine and Dentistry, University of Sciences, Techniques and Technologies, Bamako, Mali, E-mails: sdoumbo@icermali.org, isagara@icermali.org, atapily@icermali.org, adjimde@icermali.org, and okd@icermali.org. Fakhri Jeddi, Parasitology and Mycology Laboratory, APHM, Marseille, France, E-mail: fakhri.jeddi@ap-hm.fr. Tommaso Manciulli, UMR MD3 IP-TPT, Aix-Marseille University, Marseille, France, and University of Pavia, Faculty of Medicine and Surgery, Pavia, Italy, E-mail: tommaso.manciulli01@universitadipavia.it.

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