ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
McManus DP, Zhang W, Li J, Bartley PB, 2003. Echinococcosis. Lancet 362: 1295–1304.
-
2.
Craig PS, McManus DP, Lightowlers MW, Chabalgoity JA, Garcia HH, Gavidia CM, Gilman RH, Gonzalez AE, Lorca M, Naquira C, 2007. Prevention and control of cystic echinococcosis. Lancet Infect Dis 7: 385–394.
-
3.
Moro P, Schantz PM, 2009. Echinococcosis: a review. Int J Infect Dis 13: 125–133.
-
4.
Eckert J, Gemmell MA, Meslin F-X, Pawlowski ZS, 2001. WHO/OIE Manual on Echinococcosis in Humans and Animals: A Public Health Problem of Global Concern. Paris, France: World Organisation for Animal Health.
-
5.
Budke CM, Deplazes P, Torgerson PR, 2006. Global socioeconomic impact of cystic echinococcosis. Emerg Infect Dis 12: 296–303.
-
6.
Brunetti E, Kern P, Vuitton DA, 2010. Expert consensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans. Acta Trop 114: 1–16.
-
7.
Brunetti E, Junghanss T, 2009. Update on cystic hydatid disease. Curr Opin Infect Dis 22: 497–502.
-
8.
Gerke V, Moss SE, 2002. Annexins: from structure to function. Physiol Rev 82: 331–371.
-
9.
Gerke V, Creutz CE, Moss SE, 2005. Annexins: linking Ca2+ signalling to membrane dynamics. Nat Rev Mol Cell Biol 6: 449–461.
-
10.
Belluoccio D, Grskovic I, Niehoff A, Schlötzer Schrehardt U, Rosenbaum S, Etich J, Frie C, Pausch F, Moss SE, Pöschl E, 2010. Deficiency of annexins A5 and A6 induces complex changes in the transcriptome of growth plate cartilage but does not inhibit the induction of mineralization. J Bone Miner Res 25: 141–153.
-
11.
Fatimathas L, Moss SE, 2010. Annexins as disease modifiers. Histol Histopathol 25: 527–532.
-
12.
Hofmann A, Osman A, Leow CY, Driguez P, McManus DP, Jones MK, 2010. Parasite annexins—new molecules with potential for drug and vaccine development. BioEssays 32: 967–976.
-
13.
Cantacessi C, Seddon JM, Miller TL, Leow CY, Thomas L, Mason L, Willis C, Walker G, Loukas A, Gasser RB, 2013. A genome-wide analysis of annexins from parasitic organisms and their vectors. Sci Rep 3: 2898.
-
14.
Patel N, Hamamouch N, Li C, Hewezi T, Hussey RS, Baum TJ, Mitchum MG, Davis EL, 2010. A nematode effector protein similar to annexins in host plants. J Exp Bot 61: 235–248.
-
15.
He L, Ren MY, Chen XQ, Wang XY, Li S, Lin JS, Liang C, Liang P, Hu Y, Lei HL, 2008. Calcium-dependent proapoptotic effect of Taenia solium metacestodes annexin B1 on human eosinophils: a novel strategy to prevent host immune response. Int J Biochem Cell Biol 40: 2151–2163.
-
16.
Tararam CA, Farias LP, Wilson RA, de Cerqueira Leite LC, 2010. Schistosoma mansoni annexin 2: molecular characterization and immunolocalization. Exp Parasitol 126: 146–155.
-
17.
He L, Ren MY, Chen XQ, Wang XY, Li S, Lin JS, Liang C, Liang P, Hu Y, Lei HL, 2014. Biochemical and immunological characterization of annexin B30 from Clonorchis sinensis excretory/secretory products. Parasitol Res 113: 2743–2755.
-
18.
Yazid S, Sinniah A, Solito E, Calder V, Flower RJ, 2013. Anti-allergic cromones inhibit histamine and eicosanoid release from activated human and murine mast cells by releasing Annexin A1. PLoS One 8: e58963.
-
19.
Bharadwaj A, Bydoun M, Holloway R, Waisman D, 2013. Annexin A2 heterotetramer: structure and function. Int J Mol Sci 14: 6259–6305.
-
20.
van Genderen HO, Kenis H, Hofstra L, Narula J, Reutelingsperger CP, 2008. Extracellular annexin A5: functions of phosphatidylserine-binding and two-dimensional crystallization. Biochim Biophys Acta 1783: 953–963.
-
21.
Nickel W, 2005. Unconventional secretory routes: direct protein export across the plasma membrane of mammalian cells. Traffic 6: 607–614.
-
22.
Virginio VG, Monteiro KM, Drumond F, de Carvalho MO, Vargas DM, Zaha A, Ferreira HB, 2012. Excretory/secretory products from in vitro-cultured Echinococcus granulosus protoscoleces. Mol Biochem Parasitol 183: 15–22.
-
23.
Aziz A, Zhang W, Li J, Loukas A, McManus DP, Mulvenna J, 2011. Proteomic characterisation of Echinococcus granulosus hydatid cyst fluid from sheep, cattle and humans. J Proteomics 74: 1560–1572.
-
24.
Dematteis S, Pirotto F, Marques J, Nieto A, Örn A, Baz A, 2001. Modulation of the cellular immune response by a carbohydrate rich fraction from Echinococcus granulosus protoscoleces in infected or immunized Balb/c mice. Parasite Immunol 23: 1–9.
-
25.
Nie HM, Xie Y, Fu Y, Yang Y, Gu XB, Wang S, Peng X, Lai W, Peng XR, Yang GY, 2013. Cloning and characterization of the fatty acid-binding protein gene from the protoscolex of Taenia multiceps. Parasitol Res 112: 1833–1839.
-
26.
Hu DD, Song XJ, Xie Y, Zhong XQ, Wang N, Zheng Y, Gu XB, Wang T, Peng XR, Yang GY, 2015. Molecular insights into a tetraspanin in the hydatid tapeworm Echinococcus granulosus. Parasit Vectors 8: 311.
-
27.
Livak KJ, Schmittgen TD, 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25: 402–408.
-
28.
Zhang Y, Wang KH, Guo YJ, Lu YM, Yan HL, Song YL, Wang F, Ding FX, Sun SH, 2007. Annexin B1 from Taenia solium metacestodes is a newly characterized member of the annexin family. Biol Chem 388: 601–610.
-
29.
Choi SH, Kwon SR, Lee EH, Kim KH, 2009. Molecular cloning, functional characterization and localization of an annexin from a fish gill fluke Microcotyle sebastis (Platyhelminthes: Monogenea). Mol Biochem Parasitol 163: 48–53.
-
30.
de la Torre Escudero E, Manzano Román R, Siles Lucas M, Pérez Sánchez R, Moyano JC, Barrera I, Oleaga A, 2012. Molecular and functional characterization of a Schistosoma bovis annexin: fibrinolytic and anticoagulant activity. Vet Parasitol 184: 25–36.
-
31.
Moss SE, Morgan RO, 2004. The annexins. Genome Biol 5: 219.
-
32.
Gao YJ, Yan HL, Ding FX, Lu YM, Sun SH, 2007. Annexin B1 at the host–parasite interface of the Taenia solium cysticercus: secreted and associated with inflammatory reaction. Acta Trop 101: 192–199.
-
33.
Morgan R, Martin Almedina S, Iglesias J, Gonzalez Florez M, Fernandez M, 2004. Evolutionary perspective on annexin calcium-binding domains. Biochim Biophys Acta 1742: 133–140.
-
34.
Konopka Postupolska D, 2007. Annexins: putative linkers in dynamic membrane-cytoskeleton interactions in plant cells. Protoplasma 230: 203–215.
-
35.
Vergnolle N, Guimbaud R, Chaussade S, Buéno L, Escourrou J, Coméra C, 2004. Annexin 1 is secreted in situ during ulcerative colitis in humans. Inflamm Bowel Dis 10: 584–592.
-
36.
Donnelly S, Moss S, 1997. Annexins in the secretory pathway. Cell Mol Life Sci 53: 533–538.
-
37.
Christmas P, Callaway J, Fallon J, Jones J, Haigler H, 1991. Selective secretion of annexin 1, a protein without a signal sequence, by the human prostate gland. J Biol Chem 266: 2499–2507.
-
38.
Faure AV, Migné C, Devilliers G, Ayala Sanmartin J, 2002. Annexin 2 “secretion” accompanying exocytosis of chromaffin cells: possible mechanisms of annexin release. Exp Cell Res 276: 79–89.
-
39.
Danielsen EM, van Deurs B, Hansen GH, 2003. “Nonclassical” secretion of annexin A2 to the lumenal side of the enterocyte brush border membrane. Biochemistry 42: 14670–14676.
-
40.
Théry C, Zitvogel L, Amigorena S, 2002. Exosomes: composition, biogenesis and function. Nat Rev Immunol 2: 569–579.
-
41.
Valapala M, Vishwanatha JK, 2011. Lipid raft endocytosis and exosomal transport facilitate extracellular trafficking of annexin A2. J Biol Chem 286: 30911–30925.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 866 | 726 | 15 |
| Full Text Views | 457 | 31 | 8 |
| PDF Downloads | 154 | 13 | 0 |
Characterization of a Secretory Annexin in Echinococcus granulosus
Xingju Song
Xingju Song
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China; Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China
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Dandan Hu
Dandan Hu
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China; Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China
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Xiuqin Zhong
Xiuqin Zhong
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China; Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China
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Ning Wang
Ning Wang
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China; Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China
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Xiaobin Gu
Xiaobin Gu
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China; Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China
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Tao Wang
Tao Wang
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China; Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China
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Xuerong Peng
Xuerong Peng
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China; Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China
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Guangyou Yang
Guangyou Yang
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China; Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China
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Cystic echinococcosis, caused by Echinococcus granulosus, is a widespread parasitic zoonosis causing economic loss and public health problems. Annexins are important proteins usually present in the plasma membrane, but previous studies have shown that an annexin B33 protein of E. granulosus (Eg-ANX) could be detected in the excretory/secretory products and cyst fluid. In this study, we cloned and characterized Eg-ANX. In silico analysis showed that the amino acid sequence of Eg-ANX was conserved and lacked any signal peptides. The phospholipid-binding activity of recombinant Eg-ANX (rEg-ANX) was tested; liposomes could bind to rEg-ANX only in the presence of Ca2+. In addition, we performed western blotting and immunohistochemical analyses to further validate the secretory properties of Eg-ANX. The protein could be detected in the cyst fluid of E. granulosus and was also present in the intermediate host tissues, which suggested that Eg-ANX might play an important role in parasite–host interaction.
Author Notes
Financial support: This study was supported by a grant from the Key Technology R&D Program of Sichuan Province, China (no. 2015NZ0041; http://www.scst.gov.cn/).
Authors' addresses: Xingju Song, Dandan Hu, Xiuqin Zhong, Ning Wang, Xiaobin Gu, Tao Wang, and Guangyou Yang, Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China, E-mails: 18728153735@163.com, 18783542005@163.com, xiuqinzhong@hotmail.com, wangningzhuhui@hotmail.com, guxiaobin198225@126.com, muhammad06@126.com, and guangyou1963@aliyun.com. Xuerong Peng, Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Ya'an, China, E-mail: 276361511@qq.com.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
McManus DP, Zhang W, Li J, Bartley PB, 2003. Echinococcosis. Lancet 362: 1295–1304.
-
2.
Craig PS, McManus DP, Lightowlers MW, Chabalgoity JA, Garcia HH, Gavidia CM, Gilman RH, Gonzalez AE, Lorca M, Naquira C, 2007. Prevention and control of cystic echinococcosis. Lancet Infect Dis 7: 385–394.
-
3.
Moro P, Schantz PM, 2009. Echinococcosis: a review. Int J Infect Dis 13: 125–133.
-
4.
Eckert J, Gemmell MA, Meslin F-X, Pawlowski ZS, 2001. WHO/OIE Manual on Echinococcosis in Humans and Animals: A Public Health Problem of Global Concern. Paris, France: World Organisation for Animal Health.
-
5.
Budke CM, Deplazes P, Torgerson PR, 2006. Global socioeconomic impact of cystic echinococcosis. Emerg Infect Dis 12: 296–303.
-
6.
Brunetti E, Kern P, Vuitton DA, 2010. Expert consensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans. Acta Trop 114: 1–16.
-
7.
Brunetti E, Junghanss T, 2009. Update on cystic hydatid disease. Curr Opin Infect Dis 22: 497–502.
-
8.
Gerke V, Moss SE, 2002. Annexins: from structure to function. Physiol Rev 82: 331–371.
-
9.
Gerke V, Creutz CE, Moss SE, 2005. Annexins: linking Ca2+ signalling to membrane dynamics. Nat Rev Mol Cell Biol 6: 449–461.
-
10.
Belluoccio D, Grskovic I, Niehoff A, Schlötzer Schrehardt U, Rosenbaum S, Etich J, Frie C, Pausch F, Moss SE, Pöschl E, 2010. Deficiency of annexins A5 and A6 induces complex changes in the transcriptome of growth plate cartilage but does not inhibit the induction of mineralization. J Bone Miner Res 25: 141–153.
-
11.
Fatimathas L, Moss SE, 2010. Annexins as disease modifiers. Histol Histopathol 25: 527–532.
-
12.
Hofmann A, Osman A, Leow CY, Driguez P, McManus DP, Jones MK, 2010. Parasite annexins—new molecules with potential for drug and vaccine development. BioEssays 32: 967–976.
-
13.
Cantacessi C, Seddon JM, Miller TL, Leow CY, Thomas L, Mason L, Willis C, Walker G, Loukas A, Gasser RB, 2013. A genome-wide analysis of annexins from parasitic organisms and their vectors. Sci Rep 3: 2898.
-
14.
Patel N, Hamamouch N, Li C, Hewezi T, Hussey RS, Baum TJ, Mitchum MG, Davis EL, 2010. A nematode effector protein similar to annexins in host plants. J Exp Bot 61: 235–248.
-
15.
He L, Ren MY, Chen XQ, Wang XY, Li S, Lin JS, Liang C, Liang P, Hu Y, Lei HL, 2008. Calcium-dependent proapoptotic effect of Taenia solium metacestodes annexin B1 on human eosinophils: a novel strategy to prevent host immune response. Int J Biochem Cell Biol 40: 2151–2163.
-
16.
Tararam CA, Farias LP, Wilson RA, de Cerqueira Leite LC, 2010. Schistosoma mansoni annexin 2: molecular characterization and immunolocalization. Exp Parasitol 126: 146–155.
-
17.
He L, Ren MY, Chen XQ, Wang XY, Li S, Lin JS, Liang C, Liang P, Hu Y, Lei HL, 2014. Biochemical and immunological characterization of annexin B30 from Clonorchis sinensis excretory/secretory products. Parasitol Res 113: 2743–2755.
-
18.
Yazid S, Sinniah A, Solito E, Calder V, Flower RJ, 2013. Anti-allergic cromones inhibit histamine and eicosanoid release from activated human and murine mast cells by releasing Annexin A1. PLoS One 8: e58963.
-
19.
Bharadwaj A, Bydoun M, Holloway R, Waisman D, 2013. Annexin A2 heterotetramer: structure and function. Int J Mol Sci 14: 6259–6305.
-
20.
van Genderen HO, Kenis H, Hofstra L, Narula J, Reutelingsperger CP, 2008. Extracellular annexin A5: functions of phosphatidylserine-binding and two-dimensional crystallization. Biochim Biophys Acta 1783: 953–963.
-
21.
Nickel W, 2005. Unconventional secretory routes: direct protein export across the plasma membrane of mammalian cells. Traffic 6: 607–614.
-
22.
Virginio VG, Monteiro KM, Drumond F, de Carvalho MO, Vargas DM, Zaha A, Ferreira HB, 2012. Excretory/secretory products from in vitro-cultured Echinococcus granulosus protoscoleces. Mol Biochem Parasitol 183: 15–22.
-
23.
Aziz A, Zhang W, Li J, Loukas A, McManus DP, Mulvenna J, 2011. Proteomic characterisation of Echinococcus granulosus hydatid cyst fluid from sheep, cattle and humans. J Proteomics 74: 1560–1572.
-
24.
Dematteis S, Pirotto F, Marques J, Nieto A, Örn A, Baz A, 2001. Modulation of the cellular immune response by a carbohydrate rich fraction from Echinococcus granulosus protoscoleces in infected or immunized Balb/c mice. Parasite Immunol 23: 1–9.
-
25.
Nie HM, Xie Y, Fu Y, Yang Y, Gu XB, Wang S, Peng X, Lai W, Peng XR, Yang GY, 2013. Cloning and characterization of the fatty acid-binding protein gene from the protoscolex of Taenia multiceps. Parasitol Res 112: 1833–1839.
-
26.
Hu DD, Song XJ, Xie Y, Zhong XQ, Wang N, Zheng Y, Gu XB, Wang T, Peng XR, Yang GY, 2015. Molecular insights into a tetraspanin in the hydatid tapeworm Echinococcus granulosus. Parasit Vectors 8: 311.
-
27.
Livak KJ, Schmittgen TD, 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25: 402–408.
-
28.
Zhang Y, Wang KH, Guo YJ, Lu YM, Yan HL, Song YL, Wang F, Ding FX, Sun SH, 2007. Annexin B1 from Taenia solium metacestodes is a newly characterized member of the annexin family. Biol Chem 388: 601–610.
-
29.
Choi SH, Kwon SR, Lee EH, Kim KH, 2009. Molecular cloning, functional characterization and localization of an annexin from a fish gill fluke Microcotyle sebastis (Platyhelminthes: Monogenea). Mol Biochem Parasitol 163: 48–53.
-
30.
de la Torre Escudero E, Manzano Román R, Siles Lucas M, Pérez Sánchez R, Moyano JC, Barrera I, Oleaga A, 2012. Molecular and functional characterization of a Schistosoma bovis annexin: fibrinolytic and anticoagulant activity. Vet Parasitol 184: 25–36.
-
31.
Moss SE, Morgan RO, 2004. The annexins. Genome Biol 5: 219.
-
32.
Gao YJ, Yan HL, Ding FX, Lu YM, Sun SH, 2007. Annexin B1 at the host–parasite interface of the Taenia solium cysticercus: secreted and associated with inflammatory reaction. Acta Trop 101: 192–199.
-
33.
Morgan R, Martin Almedina S, Iglesias J, Gonzalez Florez M, Fernandez M, 2004. Evolutionary perspective on annexin calcium-binding domains. Biochim Biophys Acta 1742: 133–140.
-
34.
Konopka Postupolska D, 2007. Annexins: putative linkers in dynamic membrane-cytoskeleton interactions in plant cells. Protoplasma 230: 203–215.
-
35.
Vergnolle N, Guimbaud R, Chaussade S, Buéno L, Escourrou J, Coméra C, 2004. Annexin 1 is secreted in situ during ulcerative colitis in humans. Inflamm Bowel Dis 10: 584–592.
-
36.
Donnelly S, Moss S, 1997. Annexins in the secretory pathway. Cell Mol Life Sci 53: 533–538.
-
37.
Christmas P, Callaway J, Fallon J, Jones J, Haigler H, 1991. Selective secretion of annexin 1, a protein without a signal sequence, by the human prostate gland. J Biol Chem 266: 2499–2507.
-
38.
Faure AV, Migné C, Devilliers G, Ayala Sanmartin J, 2002. Annexin 2 “secretion” accompanying exocytosis of chromaffin cells: possible mechanisms of annexin release. Exp Cell Res 276: 79–89.
-
39.
Danielsen EM, van Deurs B, Hansen GH, 2003. “Nonclassical” secretion of annexin A2 to the lumenal side of the enterocyte brush border membrane. Biochemistry 42: 14670–14676.
-
40.
Théry C, Zitvogel L, Amigorena S, 2002. Exosomes: composition, biogenesis and function. Nat Rev Immunol 2: 569–579.
-
41.
Valapala M, Vishwanatha JK, 2011. Lipid raft endocytosis and exosomal transport facilitate extracellular trafficking of annexin A2. J Biol Chem 286: 30911–30925.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 866 | 726 | 15 |
| Full Text Views | 457 | 31 | 8 |
| PDF Downloads | 154 | 13 | 0 |