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1.
Bangladesh Disease Control Unit, Directorate General Health Service, 2013. Annual Report. Bangladesh, India: Ministry of Health and Family Welfare, Government of Bangladesh.
-
2.
Ahmed BN, Nabi SG, Rahman M, Selim S, Bashar A, Rashid MM, Lira FY, Chowdhury TA, Mondal D, 2014. Kala-azar (visceral leishmaniasis) elimination in Bangladesh: successes and challenges. Curr Trop Med Rep 1: 163–169.
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3.
Bourgeois N, Bastien P, Reynes J, Makinson A, Rouanet I, Lachaud L, 2010. “Active chronic visceral leishmaniasis” in HIV-1-infected patients demonstrated by biological and clinical long-term follow-up of 10 patients. HIV Med 11: 670–673.
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Bangladesh Disease Control Unit, Directorate General Health Service, 2013. National Guideline for Kala-azar Case Management. Bangladesh, India: Ministry of Health and Family Welfare, Government of Bangladesh.
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Srivastava P, Dayama A, Mehrotra S, Sundar S, 2010. Diagnosis of visceral leishmaniasis. Trans R Soc Trop Med Hyg 105: 1–6.
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6.
Maurya R, Singh RK, Kumar B, Salotra P, Rai M, Sundar S, 2005. Evaluation of PCR for diagnosis of Indian kala-azar and assessment of cure. J Clin Microbiol 43: 3038–3041.
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7.
Mondal D, Alvar J, Hasnain MG, Hossain MS, Ghosh D, Huda MM, Nabi SG, Sundar S, Matlashewski G, Arana B, 2014. Efficacy and safety of single-dose liposomal amphotericin B for visceral leishmaniasis in a rural public hospital in Bangladesh: a feasibility study. Lancet Glob Health 2: e51–e57.
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8.
Soldin SJ, Brugnara C, Hicks JM, 1999. Pediatric Reference Ranges, 3rd edition. Washington, DC: AACC Press.
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Hay WW, Hayward AR, Levin MJ, Sondheimer JM, 2000. Current Pediatric Diagnosis and Treatment, 15th edition. New York, NY: Lange Medical Books/McGraw Hill.
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10.
Salam MA, Mondal D, Kabir M, Haque R, 2010. Detection of urinary leishmanial antigen by latex agglutination test (KAtex) in kala-azar patients. Bangladesh J Med Sci 9: 216–222.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 431 | 324 | 25 |
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Polymerase Chain Reaction in the Diagnosis of Visceral Leishmaniasis Recurrence in the Setting of Negative Splenic Smears
Md. Golam Hasnain
Md. Golam Hasnain
International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh; Mymensingh Medical College Hospital, Mymensingh, Bangladesh
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Ariful Basher
Ariful Basher
International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh; Mymensingh Medical College Hospital, Mymensingh, Bangladesh
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Proggananda Nath
Proggananda Nath
International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh; Mymensingh Medical College Hospital, Mymensingh, Bangladesh
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Prakash Ghosh
Prakash Ghosh
International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh; Mymensingh Medical College Hospital, Mymensingh, Bangladesh
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Faria Hossain
Faria Hossain
International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh; Mymensingh Medical College Hospital, Mymensingh, Bangladesh
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Md. Shakhawat Hossain
Md. Shakhawat Hossain
International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh; Mymensingh Medical College Hospital, Mymensingh, Bangladesh
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Dinesh Mondal
Dinesh Mondal
International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh; Mymensingh Medical College Hospital, Mymensingh, Bangladesh
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This report presents two cases of visceral leishmaniasis (VL) recurrence where the microscopy of the splenic smear failed in diagnosis. However, a strong clinical suspicion compelled further evaluation by polymerase chain reaction (PCR), which validated the etiology. This short report highlights the usefulness of PCR in diagnosing cases of suspected smear-negative VL recurrence.
Author Notes
Authors' addresses: Md. Golam Hasnain, Centre for Communicable Disease, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka, Bangladesh, E-mail: hasnain.abir@gmail.com. Ariful Basher and Proggananda Nath, Department of Internal Medicine, Mymensingh Medical College Hospital, Mymensingh, Bangladesh, E-mails: ariful.dr@gmail.com and progganath@yahoo.com. Prakash Ghosh, Faria Hossain, Md. Shakhawat Hossain, and Dinesh Mondal, Centre for Nutrition and Food Security, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka, Bangladesh, E-mails: dreamix888@yahoo.com, faria109@gmail.com, realengewu@yahoo.com, and din63d@icddrb.org.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Bangladesh Disease Control Unit, Directorate General Health Service, 2013. Annual Report. Bangladesh, India: Ministry of Health and Family Welfare, Government of Bangladesh.
-
2.
Ahmed BN, Nabi SG, Rahman M, Selim S, Bashar A, Rashid MM, Lira FY, Chowdhury TA, Mondal D, 2014. Kala-azar (visceral leishmaniasis) elimination in Bangladesh: successes and challenges. Curr Trop Med Rep 1: 163–169.
-
3.
Bourgeois N, Bastien P, Reynes J, Makinson A, Rouanet I, Lachaud L, 2010. “Active chronic visceral leishmaniasis” in HIV-1-infected patients demonstrated by biological and clinical long-term follow-up of 10 patients. HIV Med 11: 670–673.
-
4.
Bangladesh Disease Control Unit, Directorate General Health Service, 2013. National Guideline for Kala-azar Case Management. Bangladesh, India: Ministry of Health and Family Welfare, Government of Bangladesh.
-
5.
Srivastava P, Dayama A, Mehrotra S, Sundar S, 2010. Diagnosis of visceral leishmaniasis. Trans R Soc Trop Med Hyg 105: 1–6.
-
6.
Maurya R, Singh RK, Kumar B, Salotra P, Rai M, Sundar S, 2005. Evaluation of PCR for diagnosis of Indian kala-azar and assessment of cure. J Clin Microbiol 43: 3038–3041.
-
7.
Mondal D, Alvar J, Hasnain MG, Hossain MS, Ghosh D, Huda MM, Nabi SG, Sundar S, Matlashewski G, Arana B, 2014. Efficacy and safety of single-dose liposomal amphotericin B for visceral leishmaniasis in a rural public hospital in Bangladesh: a feasibility study. Lancet Glob Health 2: e51–e57.
-
8.
Soldin SJ, Brugnara C, Hicks JM, 1999. Pediatric Reference Ranges, 3rd edition. Washington, DC: AACC Press.
-
9.
Hay WW, Hayward AR, Levin MJ, Sondheimer JM, 2000. Current Pediatric Diagnosis and Treatment, 15th edition. New York, NY: Lange Medical Books/McGraw Hill.
-
10.
Salam MA, Mondal D, Kabir M, Haque R, 2010. Detection of urinary leishmanial antigen by latex agglutination test (KAtex) in kala-azar patients. Bangladesh J Med Sci 9: 216–222.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 431 | 324 | 25 |
| Full Text Views | 221 | 8 | 0 |
| PDF Downloads | 51 | 10 | 0 |