Prevalence and Distribution of Leishmania RNA Virus 1 in Leishmania Parasites from French Guiana

Marine Ginouvès Ecosystèmes Amazoniens et Pathologie Tropicale–EA 3593–Labex CEBA–Medicine Department, University of French Guiana, Cayenne, French Guiana; Laboratoire Associé–Centre National de Référence Leishmania, Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana; Immunology Laboratory of Leishmaniasis, Pasteur Institute of French Guiana, Cayenne, French Guiana; Laboratory of Virus-Host Interactions, Pasteur Institute of French Guiana, Cayenne, French Guiana; Department of Biochemistry, University of Lausanne, Epalinges, Switzerland; Guianan Institute of Tropical Dermatology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana; Centre d'Investigation Clinique Epidémiologie Clinique Antilles Guyane, Cayenne General Hospital, Cayenne, French Guiana; Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana

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Stéphane Simon Ecosystèmes Amazoniens et Pathologie Tropicale–EA 3593–Labex CEBA–Medicine Department, University of French Guiana, Cayenne, French Guiana; Laboratoire Associé–Centre National de Référence Leishmania, Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana; Immunology Laboratory of Leishmaniasis, Pasteur Institute of French Guiana, Cayenne, French Guiana; Laboratory of Virus-Host Interactions, Pasteur Institute of French Guiana, Cayenne, French Guiana; Department of Biochemistry, University of Lausanne, Epalinges, Switzerland; Guianan Institute of Tropical Dermatology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana; Centre d'Investigation Clinique Epidémiologie Clinique Antilles Guyane, Cayenne General Hospital, Cayenne, French Guiana; Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana

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Eliane Bourreau Ecosystèmes Amazoniens et Pathologie Tropicale–EA 3593–Labex CEBA–Medicine Department, University of French Guiana, Cayenne, French Guiana; Laboratoire Associé–Centre National de Référence Leishmania, Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana; Immunology Laboratory of Leishmaniasis, Pasteur Institute of French Guiana, Cayenne, French Guiana; Laboratory of Virus-Host Interactions, Pasteur Institute of French Guiana, Cayenne, French Guiana; Department of Biochemistry, University of Lausanne, Epalinges, Switzerland; Guianan Institute of Tropical Dermatology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana; Centre d'Investigation Clinique Epidémiologie Clinique Antilles Guyane, Cayenne General Hospital, Cayenne, French Guiana; Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana

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Vincent Lacoste Ecosystèmes Amazoniens et Pathologie Tropicale–EA 3593–Labex CEBA–Medicine Department, University of French Guiana, Cayenne, French Guiana; Laboratoire Associé–Centre National de Référence Leishmania, Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana; Immunology Laboratory of Leishmaniasis, Pasteur Institute of French Guiana, Cayenne, French Guiana; Laboratory of Virus-Host Interactions, Pasteur Institute of French Guiana, Cayenne, French Guiana; Department of Biochemistry, University of Lausanne, Epalinges, Switzerland; Guianan Institute of Tropical Dermatology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana; Centre d'Investigation Clinique Epidémiologie Clinique Antilles Guyane, Cayenne General Hospital, Cayenne, French Guiana; Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana

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Catherine Ronet Ecosystèmes Amazoniens et Pathologie Tropicale–EA 3593–Labex CEBA–Medicine Department, University of French Guiana, Cayenne, French Guiana; Laboratoire Associé–Centre National de Référence Leishmania, Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana; Immunology Laboratory of Leishmaniasis, Pasteur Institute of French Guiana, Cayenne, French Guiana; Laboratory of Virus-Host Interactions, Pasteur Institute of French Guiana, Cayenne, French Guiana; Department of Biochemistry, University of Lausanne, Epalinges, Switzerland; Guianan Institute of Tropical Dermatology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana; Centre d'Investigation Clinique Epidémiologie Clinique Antilles Guyane, Cayenne General Hospital, Cayenne, French Guiana; Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana

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Pierre Couppié Ecosystèmes Amazoniens et Pathologie Tropicale–EA 3593–Labex CEBA–Medicine Department, University of French Guiana, Cayenne, French Guiana; Laboratoire Associé–Centre National de Référence Leishmania, Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana; Immunology Laboratory of Leishmaniasis, Pasteur Institute of French Guiana, Cayenne, French Guiana; Laboratory of Virus-Host Interactions, Pasteur Institute of French Guiana, Cayenne, French Guiana; Department of Biochemistry, University of Lausanne, Epalinges, Switzerland; Guianan Institute of Tropical Dermatology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana; Centre d'Investigation Clinique Epidémiologie Clinique Antilles Guyane, Cayenne General Hospital, Cayenne, French Guiana; Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana

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Mathieu Nacher Ecosystèmes Amazoniens et Pathologie Tropicale–EA 3593–Labex CEBA–Medicine Department, University of French Guiana, Cayenne, French Guiana; Laboratoire Associé–Centre National de Référence Leishmania, Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana; Immunology Laboratory of Leishmaniasis, Pasteur Institute of French Guiana, Cayenne, French Guiana; Laboratory of Virus-Host Interactions, Pasteur Institute of French Guiana, Cayenne, French Guiana; Department of Biochemistry, University of Lausanne, Epalinges, Switzerland; Guianan Institute of Tropical Dermatology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana; Centre d'Investigation Clinique Epidémiologie Clinique Antilles Guyane, Cayenne General Hospital, Cayenne, French Guiana; Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana

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Magalie Demar Ecosystèmes Amazoniens et Pathologie Tropicale–EA 3593–Labex CEBA–Medicine Department, University of French Guiana, Cayenne, French Guiana; Laboratoire Associé–Centre National de Référence Leishmania, Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana; Immunology Laboratory of Leishmaniasis, Pasteur Institute of French Guiana, Cayenne, French Guiana; Laboratory of Virus-Host Interactions, Pasteur Institute of French Guiana, Cayenne, French Guiana; Department of Biochemistry, University of Lausanne, Epalinges, Switzerland; Guianan Institute of Tropical Dermatology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana; Centre d'Investigation Clinique Epidémiologie Clinique Antilles Guyane, Cayenne General Hospital, Cayenne, French Guiana; Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana

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Ghislaine Prévot Ecosystèmes Amazoniens et Pathologie Tropicale–EA 3593–Labex CEBA–Medicine Department, University of French Guiana, Cayenne, French Guiana; Laboratoire Associé–Centre National de Référence Leishmania, Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana; Immunology Laboratory of Leishmaniasis, Pasteur Institute of French Guiana, Cayenne, French Guiana; Laboratory of Virus-Host Interactions, Pasteur Institute of French Guiana, Cayenne, French Guiana; Department of Biochemistry, University of Lausanne, Epalinges, Switzerland; Guianan Institute of Tropical Dermatology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana; Centre d'Investigation Clinique Epidémiologie Clinique Antilles Guyane, Cayenne General Hospital, Cayenne, French Guiana; Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana

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DOI:
https://doi.org/10.4269/ajtmh.15-0419
Volume/Issue:
Volume 94: Issue 1
Page(s):
102–106
Restricted access
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In South America, the presence of the Leishmania RNA virus type 1 (LRV1) was described in Leishmania guyanensis and Leishmania braziliensis strains. The aim of this study was to determine the prevalence distribution of LRV1 in Leishmania isolates in French Guiana given that, in this French overseas department, most Leishmania infections are due to these parasite species. The presence of the virus was observed in 74% of Leishmania spp. isolates, with a highest presence in the internal areas of the country.

Author Notes

* Address correspondence to Marine Ginouvès, Ecosystèmes Amazoniens et Pathologie Tropicale–EA 3593–Labex CEBA–Medicine Department, University of French Guiana, Campus Saint Denis, Avenue d'Estrees, Cayenne 97300, French Guiana. E-mail: marine.ginouves@univ-guyane.fr

Financial support: This work was supported by the University of the French West Indies and French Guiana and the Ministère Français de l'Enseignement Supérieur et de la Recherche Scientifique. It has benefited from an Investissement d'Avenir grant managed by Agence Nationale de la Recherche (CEBA, reference no. ANR-10-LABX-25-01).

Conflict of interest: Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.

Authors' addresses: Marine Ginouvès and Stéphane Simon, Ecosystèmes Amazoniens et Pathologie Tropicale–EA 3593–Labex CEBA–Medicine Department, University of French Guiana, Cayenne, French Guiana, and Laboratoire Associé–Centre National de Référence Leishmania, Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana, E-mails: marine.ginouves@univ-guyane.fr and stephane.simon@guyane.univ-ag.fr. Eliane Bourreau, Immunology Laboratory of Leishmaniasis, Pasteur Institute of French Guiana, Cayenne, French Guiana, E-mail: ebourreau@pasteur-cayenne.fr. Vincent Lacoste, Laboratory of Virus Hosts Interaction, Pasteur Institute of French Guiana, Cayenne, French Guiana, E-mail: vlacoste@pasteur-cayenne.fr. Catherine Ronet, Department of Biochemistry, University of Lausanne, Epalinges, Switzerland, E-mail: catherine.ronet@unil.ch. Pierre Couppié, Ecosystemes Amazoniens et Pathologie Tropicale–EA 3593–Labex CEBA–Medicine Department, University of French Guiana, Cayenne, French Guiana, and Guianan Institute of Tropical Dermatology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana, E-mail: pierre.couppie@ch-cayenne.fr. Mathieu Nacher, Ecosystemes Amazoniens et Pathologie Tropicale–EA 3593–Labex CEBA–Medicine Department, University of French Guiana, Cayenne, French Guiana, and Centre d'Investigation Clinique Epidémiologie Clinique Antilles Guyane, Cayenne General Hospital, Cayenne, French Guiana, E-mail: mathieu.nacher@ch-cayenne.fr. Magalie Demar, Ecosystemes Amazoniens et Pathologie Tropicale–EA 3593–Labex CEBA–Medicine Department, University of French Guiana, Cayenne, French Guiana, Laboratoire Associé–Centre National de Référence Leishmania, Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana, and Laboratory of Parasitology and Mycology, Centre Hospitalier Andrée Rosemon, Cayenne, French Guiana, E-mail: magalie.demar@ch-cayenne.fr. Ghislaine Prévot, Ecosystemes Amazoniens et Pathologie Tropicale–EA 3593–Labex CEBA–Medicine Department, University of French Guiana, Cayenne, French Guiana, E-mail: fac.prevot@gmail.com.

Copyright:
© The American Society of Tropical Medicine and Hygiene 2016
Received:
05 Jun 2015
|
Accepted:
20 Sep 2015
|
Published Online:
06 Jan 2016
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Online ISSN:
1476-1645
  • 1.

    Miller JH, Schwartzwelter JC, 1960. Virus-like particles in an Entamoeba histolytica trophozoite. J Parasitol 46: 523–524.

  • 2.

    Molyneux DH, 1974. Virus-like particles in Leishmania parasites. Nature 249: 588–589.

  • 3.

    Diamond LS, Mattern CF, 1976. Protozoal viruses. Adv Virus Res 20: 87–112.

  • 4.

    Wang AL, Wang CC, 1986. Discovery of a specific double-stranded RNA virus in Giardia lamblia. Mol Biochem Parasitol 21: 269–276.

  • 5.

    Wang AL, Wang CC, 1986. The double-stranded RNA in Trichomonas vaginalis may originate from virus-like particles. Proc Natl Acad Sci USA 83: 7956–7960.

    • Search Google Scholar
    • Export Citation
  • 6.

    Tipper DJ, Bostian KA, 1984. Double-stranded ribonucleic acid killer systems in yeasts. Microbiol Rev 48: 125–156.

  • 7.

    Tarr PI, Aline RF Jr, Smiley BL, Scholler J, Keithly J, Stuart K, 1988. LR1: a candidate RNA virus of Leishmania. Proc Natl Acad Sci USA 85: 9572–9575.

    • Search Google Scholar
    • Export Citation
  • 8.

    Cadd TL, Keenan MC, Patterson JL, 1993. Detection of Leishmania RNA virus 1 proteins. J Virol 67: 5647–5650.

  • 9.

    Zangger H, Hailu A, Desponds C, Lye LF, Akopyants NS, Dobson DE, Ronet C, Ghalib H, Beverley SM, Fasel N, 2014. Leishmania aethiopica field isolates bearing an endosymbiontic dsRNA virus induce pro-inflammatory cytokine response. PLoS Negl Trop Dis 8: e2836.

    • Search Google Scholar
    • Export Citation
  • 10.

    Guilbride L, Myler PJ, Stuart K, 1992. Distribution and sequence divergence of LRV1 viruses among different Leishmania species. Mol Biochem Parasitol 54: 101–104.

    • Search Google Scholar
    • Export Citation
  • 11.

    Widmer G, Dooley S, 1995. Phylogenetic analysis of Leishmania RNA virus and Leishmania suggests ancient virus-parasite association. Nucleic Acids Res 23: 2300–2304.

    • Search Google Scholar
    • Export Citation
  • 12.

    Stuart KD, Weeks R, Guilbride L, Myler PJ, 1992. Molecular organization of Leishmania RNA virus 1. Proc Natl Acad Sci USA 89: 8596–8600.

  • 13.

    Scheffter SM, Ro YT, Chung IK, Patterson JL, 1995. The complete sequence of Leishmania RNA virus LRV2-1, a virus of an old world parasite strain. Virology 212: 84–90.

    • Search Google Scholar
    • Export Citation
  • 14.

    Scheffter S, Widmer G, Patterson JL, 1994. Complete sequence of Leishmania RNA virus 1–4 and identification of conserved sequences. Virology 199: 479–483.

    • Search Google Scholar
    • Export Citation
  • 15.

    Rotureau B, Ravel C, Nacher M, Couppié P, Curtet I, Dedet JP, Carme B, 2006. Molecular epidemiology of Leishmania (Viannia) guyanensis in French Guiana. J Clin Microbiol 44: 468–473.

    • Search Google Scholar
    • Export Citation
  • 16.

    Simon S, Veron V, Carme B, 2010. Leishmania spp. identification by polymerase chain reaction–restriction fragment length polymorphism analysis and its applications in French Guiana. Diagn Microbiol Infect Dis 66: 175–180.

    • Search Google Scholar
    • Export Citation
  • 17.

    Ives A, Ronet C, Prevel F, Ruzzante G, Fuertes-Marraco S, Schutz F, Zangger H, Revaz-Breton M, Lye L, Hickerson SM, Beverley SM, Acha-Orbea H, Launois P, Fasel N, Masina S, 2011. Leishmania RNA virus controls the severity of mucocutaneous leishmaniasis. Science 331: 775–778.

    • Search Google Scholar
    • Export Citation
  • 18.

    Salinas G, Zamora M, Stuart K, Saravia N, 1996. Leishmania RNA viruses in Leishmania of the Viannia subgenus. Am J Trop Med Hyg 54: 425–429.

  • 19.

    Saiz M, Llanos-Cuentas A, Echevarria J, Roncal N, Cruz M, Muniz MT, Lucas C, Wirth DF, Scheffter S, Magill AJ, Patterson JL, 1998. Short report: detection of Leishmania virus in human biopsy samples of leishmaniasis from Peru. Am J Trop Med Hyg 58: 192–194.

    • Search Google Scholar
    • Export Citation
  • 20.

    Ogg MM, Carrion R Jr, Botelho AC, Mayrink W, Correa-Oliveira R, Patterson JL, 2003. Short report: quantification of Leishmania virus RNA in clinical samples and its possible role in pathogenesis. Am J Trop Med Hyg 69: 309–313.

    • Search Google Scholar
    • Export Citation
  • 21.

    Bourreau E, Ginouves M, Prévot G, Hartley M, Gangneux J, Robert-Gangneux F, Dufour J, Marie DS, Bertolotti A, Pratlong F, Martin R, Schütz F, Couppié P, Fasel N, Ronet C, 2015. Leishmania RNA virus presence in L. guyanensis parasites increases the risk of first-line treatment failure and symptomatic relapse. J Infect Dis. [epub ahead of print].

    • Search Google Scholar
    • Export Citation
  • 22.

    Pereira L de O, Maretti-Mira AC, Rodrigues KM, Lima RB, Oliveira-Neto MP, Cupolillo E, Pirmez C, de Oliveira MP, 2013. Severity of tegumentary leishmaniasis is not exclusively associated with Leishmania RNA virus 1 infection in Brazil. Mem Inst Oswaldo Cruz 108: 665–667.

    • Search Google Scholar
    • Export Citation
  • 23.

    Rotureau B, Gaborit P, Issaly J, Carinci R, Fouque F, Carme B, 2006. Diversity and ecology of sand flies (Diptera: Psychodidae: Phlebotominae) in coastal French Guiana. Am J Trop Med Hyg 75: 62–69.

    • Search Google Scholar
    • Export Citation
  • 24.

    Fouque F, Gaborit P, Issaly J, Carinci R, Gantier JC, Ravel C, Dedet JP, 2007. Phlebotomine sand flies (Diptera: Psychodidae) associated with changing patterns in the transmission of the human cutaneous leishmaniasis in French Guiana. Mem Inst Oswaldo Cruz 102: 35–40.

    • Search Google Scholar
    • Export Citation
  • 25.

    Croft SL, Molyneux DH, 1979. Studies on the ultrastructure, virus-like particles and infectivity of Leishmania hertigi. Ann Trop Med Parasitol 73: 213–226.

    • Search Google Scholar
    • Export Citation
  • 26.

    Ronet C, Beverley SM, Fasel N, 2011. Muco-cutaneous leishmaniasis in the new world: the ultimate subversion. Virulence 2: 547–552.

  • 27.

    Ives A, Masina S, Castiglioni P, Prevel F, Revaz-Breton M, Hartley MA, Launois P, Fasel N, Ronet C, 2014. MyD88 and TLR9 dependent immune responses mediate resistance to Leishmania guyanensis infections, irrespective of Leishmania RNA virus burden. PLoS One 9: e96766.

    • Search Google Scholar
    • Export Citation
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