High Pathogenicity of Influenza A (H10N8) Virus in Mice

Haiying Chen Department of Infectious Diseases, Nanchang Center for Disease Control and Prevention, Nanchang, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou, China

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Lihong Huang Department of Infectious Diseases, Nanchang Center for Disease Control and Prevention, Nanchang, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou, China

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Hui Li Department of Infectious Diseases, Nanchang Center for Disease Control and Prevention, Nanchang, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou, China

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Xianfeng Zhou Department of Infectious Diseases, Nanchang Center for Disease Control and Prevention, Nanchang, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou, China

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Huanan Li Department of Infectious Diseases, Nanchang Center for Disease Control and Prevention, Nanchang, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou, China

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Na Sun Department of Infectious Diseases, Nanchang Center for Disease Control and Prevention, Nanchang, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou, China

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Wenbao Qi Department of Infectious Diseases, Nanchang Center for Disease Control and Prevention, Nanchang, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou, China

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Chencheng Xiao Department of Infectious Diseases, Nanchang Center for Disease Control and Prevention, Nanchang, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou, China

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Xiansheng Ni Department of Infectious Diseases, Nanchang Center for Disease Control and Prevention, Nanchang, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou, China

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Mingbin Liu Department of Infectious Diseases, Nanchang Center for Disease Control and Prevention, Nanchang, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou, China

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Ming Liao Department of Infectious Diseases, Nanchang Center for Disease Control and Prevention, Nanchang, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou, China

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Three human cases of H10N8 virus infections were initially reported in China in late 2013 and early 2014, two of which were fatal. This was the first time the H10N8 subtype has been detected in humans, and the pathogenicity of this virus remains under characterized. We first assessed its pathogenicity by infecting BALB/c mice with two H10N8 isolates, A/Jiangxi-Donghu/346-1/2013 and A/Chicken/Jiangxi/102/2013. The human isolate (H346-1) demonstrated stronger capability of replication and induced higher cytokine response in vivo than the chicken isolate (C102). In addition, H346-1 was fatal to mice, while all mice (N = 14) in C102-infected group survived during the infection course without weight loss. We hypothesized that the 627K mutation in the PB2 gene (PB2-K627) in H346-1 was associated with high pathogenicity in mice. Taken together, this study based on mouse model provides some insight into understanding the pathogenicity of the emerging viruses in mammals.

Author Notes

* Address correspondence to Ming Liao, College of Veterinary Medicine, South China Agricultural University, 483 Wushan Road, Tianhe District, Guangzhou, Guangdong Province 510642, China. E-mail: nccdczxf@126.com
† These authors contributed equally to this article.

Financial support: This research was supported by the National Natural Science Foundation of China (No. 81460302), Major Science and Technological Project of Jiangxi Province (No. 20143ACG70004), Science and Technology Planning Project of Guangdong Province (No. 20140224, 2013B020202001), Development Program for Excellent Young Teachers in Guangdong Province (No. Yq2013025) and Science and technology nova Program of Pearl River of Guangzhou (No. 2014J2200072).

Authors' addresses: Haiying Chen, Hui Li, Xianfeng Zhou, Xiansheng Ni, and Mingbin Liu, Department of Infectious Diseases, Nanchang Center for Disease Control and Prevention, Nanchang, China, E-mails: nccdcchy@126.com, nccdcyjb@163.com, xfzhou-nccdc@hotmail.com, nxsh94@163.com, and mingbinliu@126.com. Lihong Huang, Huanan Li, Na Sun, Wenbao Qi, Chencheng Xiao, and Ming Liao, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China, E-mails: huanglihongtxs@163.com, huananli@yeah.net, 1018989253@qq.com, qiwenbao@scau.edu.cn, 305639447@qq.com, and nccdczxf@126.com.

  • 1.

    García-Sastre A, Schmolke M, 2014. Avian influenza A H10N8–a virus on the verge? Lancet 383: 676677.

  • 2.

    Kuiken T, Holmes EC, McCauley J, Rimmelzwaan GF, Williams CS, Grenfell BT, 2006. Host species barriers to influenza virus infections. Science 312: 394397.

    • Search Google Scholar
    • Export Citation
  • 3.

    Webster RG, Peiris M, Chen H, Guan Y, 2006. H5N1 outbreaks and enzootic influenza. Emerg Infect Dis 12: 38.

  • 4.

    Wei SH, Yang JR, Wu HS, Chang MC, Lin JS, Lin CY, Liu YL, Lo YC, Yang CH, Chuang JH, Lin MC, Chung WC, Liao CH, Lee MS, Huang WT, Chen PJ, Liu MT, Chang FY, 2013. Human infection with avian influenza A H6N1 virus: an epidemiological analysis. Lancet Respir Med 1: 771778.

    • Search Google Scholar
    • Export Citation
  • 5.

    Peiris M, Yuen KY, Leung CW, Chan KH, Ip PL, Lai RW, Orr WK, Shortridge KF, 1999. Human infection with influenza H9N2. Lancet 354: 916917.

  • 6.

    Belser JA, Bridges CB, Katz JM, Tumpey TM, 2009. Past, present, and possible future human infection with influenza virus A subtype H7. Emerg Infect Dis 15: 859865.

    • Search Google Scholar
    • Export Citation
  • 7.

    To KK, Tsang AK, Chan JF, Cheng VC, Chen H, Yuen KY, 2014. Emergence in China of human disease due to avian influenza A (H10N8)–cause for concern? J Infect 68: 205215.

    • Search Google Scholar
    • Export Citation
  • 8.

    Chen H, Yuan H, Gao R, Zhang J, Wang D, Xiong Y, Fan G, Yang F, Li X, Zhou J, Zou S, Yang L, Chen T, Dong L, Bo H, Zhao X, Zhang Y, Lan Y, Bai T, Dong J, Li Q, Wang S, Zhang Y, Li H, Gong T, Shi Y, Ni X, Li J, Zhou J, Fan J, Wu J, Zhou X, Hu M, Wan J, Yang W, Li D, Wu G, Feng Z, Gao GF, Wang Y, Jin Q, Liu M, Shu Y, 2014. Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection: a descriptive study. Lancet 383: 714721.

    • Search Google Scholar
    • Export Citation
  • 9.

    Qi W, Zhou X, Shi W, Huang L, Xia W, Liu D, Li H, Chen S, Lei F, Cao L, Wu J, He F, Song W, Li Q, Li H, Liao M, Liu M, 2014. Genesis of the novel human-infecting influenza A (H10N8) virus and potential genetic diversity of the virus in poultry, China. Euro Surveill 19: 20841.

    • Search Google Scholar
    • Export Citation
  • 10.

    Reed LJ, Muench H, 1938. A simple method of estimating fifty percent endpoints. Am J Hyg 27: 493497.

  • 11.

    Hatta M, Gao P, Halfmann P, Kawaoka Y, 2001. Molecular basis for high virulence of Hong Kong H5N1 influenza A viruses. Science 293: 18401842.

  • 12.

    Li X, Qi W, He J, Ning Z, Hu Y, Tian J, Jiao P, Xu C, Chen J, Richt J, Ma W, Liao M, 2012. Molecular basis of efficient replication and pathogenicity of H9N2 avian influenza viruses in mice. PLoS One 7: e40118.

    • Search Google Scholar
    • Export Citation
  • 13.

    Mok CK, Lee HH, Lestra M, Nicholls JM, Chan MC, Sia SF, Zhu H, Poon LL, Guan Y, Peiris JS, 2014. Amino acid substitutions in polymerase basic protein 2 gene contributes to the pathogenicity of the novel A/H7N9 influenza virus in mammalian hosts. J Virol 88: 35683576.

    • Search Google Scholar
    • Export Citation
  • 14.

    Arzey GG, Kirkland PD, Arzey KE, Frost M, Maywood P, Conaty S, Hurt AC, Deng YM, Iannello P, Barr I, Dwyer DE, Ratnamohan M, McPhie K, Selleck P, 2012. Influenza virus A (H10N7) in chickens and poultry abattoir workers, Australia. Emerg Infect Dis 18: 814816.

    • Search Google Scholar
    • Export Citation
  • 15.

    Beare AS, Webster RG, 1991. Replication of avian influenza viruses in humans. Arch Virol 119: 3742.

  • 16.

    Zohari S, Neimanis A, Härkönen T, Moraeus C, Valarcher JF, 2014. Avian influenza A (H10N7) virus involvement in mass mortality of harbour seals (Phoca vitulina) in Sweden, March through October 2014. Euro Surveill 19: 20967.

    • Search Google Scholar
    • Export Citation
  • 17.

    Liu M, Li X, Yuan H, Zhou J, Wu J, Bo H, Xia W, Xiong Y, Yang L, Gao R, Guo J, Huang W, Zhang Y, Zhao X, Zou X, Chen T, Wang D, Li Q, Wang S, Chen S, Hu M, Ni X, Gong T, Shi Y, Li J, Zhou J, Cai J, Xiao Z, Zhang W, Sun J, Li D, Wu G, Feng Z, Wang Y, Chen H, Shu Y, 2015. Genetic diversity of avian influenza A (H10N8) virus in live poultry markets and its association with human infections in China. Sci Rep 5: 7632.

    • Search Google Scholar
    • Export Citation
  • 18.

    Ni X, He F, Hu M, Zhou X, Wang B, Feng C, Wu Y, Li Y, Tu J, Li H, Liu M, Chen H, Chen S, 2015. Investigation of avian influenza virus in poultry and wild birds due to novel avian-origin influenza A (H10N8) in Nanchang City, China. Microbes Infect 17: 4853.

    • Search Google Scholar
    • Export Citation
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