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Production of Toxocara cati TES-120 Recombinant Antigen and Comparison with its T. canis Homolog for Serodiagnosis of Toxocariasis

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  • Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia; Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; Department of Medical Parasitology and Mycology, School of Public Health and National Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran

Toxocariasis is a cosmopolitan zoonotic disease caused by the infective larvae of Toxocara canis and T. cati. Diagnosis in humans is usually based on clinical symptoms and serology. Immunoglobulin G (IgG)-enzyme-linked immunosorbent assay kits using T. canis excretory–secretory (TES) larval antigens are commonly used for serodiagnosis. Differences in the antigens of the two Toxocara species may influence the diagnostic sensitivity of the test. In this study, T. cati recombinant TES-120 (rTES-120) was cloned, expressed, and compared with its T. canis homolog in an IgG4-western blot. The diagnostic sensitivity and specificity of T. cati rTES-120 were 70% (33/47) and 100% (39/39), respectively. T. canis rTES-120 showed 57.4% sensitivity and 94.4% specificity. When the results of assays using rTES-120 of both species were considered, the diagnostic sensitivity was 76%. This study shows that using antigens from both Toxocara species may improve the serodiagnosis of toxocariasis.

Author Notes

* Address correspondence to Rahmah Noordin, Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Penang, Malaysia, E-mail: rahmah8485@gmail.com or Seyed Mahmoud Sadjjadi, Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran, E-mail: smsadjjadi@sums.ac.ir.

Financial support: This study was funded by the following research grants: Malaysian Cradle Fund no. 304.CIPPM.650564.C117, USM Short Term Grant no. 304.CIPPM.6311089, and SUMS Grant no. 5790.

Authors' addresses: Farzaneh Zahabiun and Seyed Mahmoud Sadjjadi, Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran, E-mails: zahabiun@gmail.com and smsadjjadi@sums.ac.ir. Muhammad Hafiznur Yunus, Anizah Rahumatullah, Syazwan Saidin, and Rahmah Noordin, Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia, E-mails: mhn1016@yahoo.com, anirah82@gmail.com, syazwan_saidin@yahoo.com, and rahmah8485@gmail.com. Falaki Moghaddam, MH Department of Medical Parasitology and Mycology, School of Public Health and National Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran, E-mail: falakimoghaddam@yahoo.com.

Reprint requests: Rahmah Noordin, Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Penang, Malaysia, E-mail: rahmah8485@gmail.com, Tel: +6046534854, or Seyed Mahmoud Sadjjadi, Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran, E-mail: smsadjjadi@sums.ac.ir.

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