Comparison of Fluorescence Microscopy and Different Growth Media Culture Methods for Acanthamoeba Keratitis Diagnosis

Avi Peretz Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel; Clinical Microbiology Laboratory, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel

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Yuval Geffen Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel; Clinical Microbiology Laboratory, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel

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Soergiu D. Socea Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel; Clinical Microbiology Laboratory, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel

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Nina Pastukh Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel; Clinical Microbiology Laboratory, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel

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Shmuel Graffi Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel; Clinical Microbiology Laboratory, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Rambam Health Care Campus, Haifa, Israel; Department of Ophthalmology, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel

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DOI:
https://doi.org/10.4269/ajtmh.15-0193
Volume/Issue:
Volume 93: Issue 2
Page(s):
316–318
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Acanthamoeba keratitis (AK), a potentially blinding infection of the cornea, is caused by a free-living protozoan. Culture and microscopic examination of corneal scraping tissue material is the conventional method for identifying Acanthamoeba. In this article, we compared several methods for AK diagnosis of 32 patients: microscopic examination using fluorescent dye, specific culture on growth media—non-nutrient agar (NNA), culture on liquid growth media—peptone yeast glucose (PYG), and TYI-S-33. AK was found in 14 patients. Thirteen of the specimens were found AK positive by fluorescence microscopic examination, 11 specimens were found AK positive on PYG growth media, and 9 specimens were found AK positive on TYI-S-33 growth media. Only five specimens were found AK positive on NNA growth media. Therefore, we recommend using fluorescence microscopy technique and culture method, especially PYG liquid media.

Author Notes

* Address correspondence to Avi Peretz, Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Hanna Senesh 818/2, Tiberias, Israel. E-mail: aperetz@poria.health.gov.il

Authors' addresses: Avi Peretz and Nina Pastukh, Clinical Microbiology Laboratory, Poriya Medical Center, Tiberias, Israel, Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel, E-mails: aperetz@poria.health.gov.il and npastukh@poria.health.gov.il. Shmuel Graffi, Department of Ophthalmology, Baruch Padeh Medical Center, Poriya, Tiberias, Israel, affiliated with the Faculty of Medicine, Bar Ilan University, Galilee, Israel, E-mail: sgraffi@walla.com. Yuval Geffen and Soergiu D. Socea, Clinical Microbiology Laboratory, Rambam Medical Center, Haifa, Israel, E-mails: y_geffen@rambam.health.gov.il and sergiusoc@gmail.com.

Copyright:
© The American Society of Tropical Medicine and Hygiene 2015
Received:
11 Mar 2015
|
Accepted:
07 Apr 2015
|
Published Online:
05 Aug 2015
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Online ISSN:
1476-1645
  • 1.

    Khan NA, 2006. Acanthamoeba: biology and increasing importance in human health. FEMS Microbiol Rev 30: 564–595.

  • 2.

    Visvesvara GS, Moura H, Schuster FL, 2007. Pathogenic and opportunistic free‐living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea. FEMS Immunol Med Microbiol 50: 1–26.

    • Search Google Scholar
    • Export Citation
  • 3.

    Baig AM, Zuberi H, Khan NA, 2014. Recommendations for the management of Acanthamoeba keratitis. J Med Microbiol 63: 770–771.

  • 4.

    Radford CF, Minassian DC, Dart JKG, 2002. Acanthamoeba keratitis in England and Wales: incidence, outcome, and risk factors. Br J Ophthalmol 86: 536–542.

    • Search Google Scholar
    • Export Citation
  • 5.

    Dart JKG, Saw VPJ, Kilvington S, 2009. Acanthamoeba keratitis: diagnosis and treatment update 2009. Am J Ophthalmol 148: 487–499.

  • 6.

    Sharma S, Garg P, Rao GN, 2000. Patient characteristics, diagnosis, and treatment of non-contact lens related Acanthamoeba keratitis. Br J Ophthalmol 84: 1103–1108.

    • Search Google Scholar
    • Export Citation
  • 7.

    Mathers WD, Nelson SE, Lane JL, Wilson ME, Allen RC, Folberg R, 2000. Confirmation of confocal microscopy diagnosis of Acanthamoeba keratitis using polymerase chain reaction analysis. Arch Ophthalmol 118: 178–183.

    • Search Google Scholar
    • Export Citation
  • 8.

    Schroeder JM, Booton GC, Hay J, Niszl IA, Seal DV, Markus MB, Fuerst PA, Byers TJ, 2001. Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of Acanthamoebae from humans with keratitis and from sewage sludge. J Clin Microbiol 39: 1903–1911.

    • Search Google Scholar
    • Export Citation
  • 9.

    Thompson PP, Kowalski RP, Shanks RM, Gordon YJ, 2008. Validation of real-time PCR for laboratory diagnosis of Acanthamoeba keratitis. J Clin Microbiol 46: 3232–3236.

    • Search Google Scholar
    • Export Citation
  • 10.

    Borin S, Feldman I, Ken-Dror S, Briscoe D, 2013. Rapid diagnosis of Acanthamoeba keratitis using non-nutrient agar with a lawn of E. coli. J Ophthalmic Inflamm Infect 3: 1–2.

    • Search Google Scholar
    • Export Citation
  • 11.

    Pérez-Santonja JJ, Kilvington S, Hughes R, Tufail A, Matheson M, Dart JK, 2003. Persistently culture positive Acanthamoeba keratitis: in vivo resistance and in vitro sensitivity. Ophthalmology 110: 1593–1600.

    • Search Google Scholar
    • Export Citation
  • 12.

    Booton GC, Joslin CE, Shoff M, Tu EY, Kelly DJ, Fuerst PA, 2009. Genotypic identification of Acanthamoeba sp. isolates associated with an outbreak of Acanthamoeba keratitis (AK). Cornea 28: 673.

    • Search Google Scholar
    • Export Citation
  • 13.

    Graffi S, Peretz A, Jabaly H, Naftali M, 2013. Acanthamoeba keratitis. Isr Med Assoc J 15: 182–185.

  • 14.

    Yera H, Zamfir O, Bourcier T, Ancelle T, Batellier L, Dupouy-Camet J, Chaumeil C, 2007. Comparison of PCR, microscopic examination and culture for the early diagnosis and characterization of Acanthamoeba isolates from ocular infections. Eur J Clin Microbiol Infect Dis 26: 221–224.

    • Search Google Scholar
    • Export Citation
  • 15.

    Boggild AK, Martin DS, Lee TY, Yu B, Low DE, 2009. Laboratory diagnosis of amoebic keratitis: comparison of four diagnostic methods for different types of clinical specimens. J Clin Microbiol 47: 1314–1318.

    • Search Google Scholar
    • Export Citation
  • 16.

    Lorenzo-Morales J, Khan NA, Walochnik J, 2015. An update on Acanthamoeba keratitis: diagnosis, pathogenesis and treatment. Parasite 22: 10.

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