Sampling Host-Seeking Anthropophilic Mosquito Vectors in West Africa: Comparisons of an Active Human-Baited Tent-Trap Against Gold Standard Methods

Benjamin J. Krajacich Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado; Special Programs, Infoscitex Corporation, Littleton, Massachusetts; The Liberian Institute for Biomedical Research, Monrovia, Liberia; Vector Biology Research Program Naval Medical Research Unit No. 3, Cairo, Egypt; Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, Burkina Faso

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Jeremiah R. Slade Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado; Special Programs, Infoscitex Corporation, Littleton, Massachusetts; The Liberian Institute for Biomedical Research, Monrovia, Liberia; Vector Biology Research Program Naval Medical Research Unit No. 3, Cairo, Egypt; Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, Burkina Faso

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Robert F. Mulligan Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado; Special Programs, Infoscitex Corporation, Littleton, Massachusetts; The Liberian Institute for Biomedical Research, Monrovia, Liberia; Vector Biology Research Program Naval Medical Research Unit No. 3, Cairo, Egypt; Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, Burkina Faso

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Brendan LaBrecque Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado; Special Programs, Infoscitex Corporation, Littleton, Massachusetts; The Liberian Institute for Biomedical Research, Monrovia, Liberia; Vector Biology Research Program Naval Medical Research Unit No. 3, Cairo, Egypt; Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, Burkina Faso

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Haoues Alout Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado; Special Programs, Infoscitex Corporation, Littleton, Massachusetts; The Liberian Institute for Biomedical Research, Monrovia, Liberia; Vector Biology Research Program Naval Medical Research Unit No. 3, Cairo, Egypt; Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, Burkina Faso

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Nathan D. Grubaugh Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado; Special Programs, Infoscitex Corporation, Littleton, Massachusetts; The Liberian Institute for Biomedical Research, Monrovia, Liberia; Vector Biology Research Program Naval Medical Research Unit No. 3, Cairo, Egypt; Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, Burkina Faso

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Jacob I. Meyers Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado; Special Programs, Infoscitex Corporation, Littleton, Massachusetts; The Liberian Institute for Biomedical Research, Monrovia, Liberia; Vector Biology Research Program Naval Medical Research Unit No. 3, Cairo, Egypt; Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, Burkina Faso

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Lawrence S. Fakoli III Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado; Special Programs, Infoscitex Corporation, Littleton, Massachusetts; The Liberian Institute for Biomedical Research, Monrovia, Liberia; Vector Biology Research Program Naval Medical Research Unit No. 3, Cairo, Egypt; Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, Burkina Faso

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Fatorma K. Bolay Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado; Special Programs, Infoscitex Corporation, Littleton, Massachusetts; The Liberian Institute for Biomedical Research, Monrovia, Liberia; Vector Biology Research Program Naval Medical Research Unit No. 3, Cairo, Egypt; Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, Burkina Faso

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Doug E. Brackney Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado; Special Programs, Infoscitex Corporation, Littleton, Massachusetts; The Liberian Institute for Biomedical Research, Monrovia, Liberia; Vector Biology Research Program Naval Medical Research Unit No. 3, Cairo, Egypt; Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, Burkina Faso

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Timothy A. Burton Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado; Special Programs, Infoscitex Corporation, Littleton, Massachusetts; The Liberian Institute for Biomedical Research, Monrovia, Liberia; Vector Biology Research Program Naval Medical Research Unit No. 3, Cairo, Egypt; Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, Burkina Faso

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Jonathan A. Seaman Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado; Special Programs, Infoscitex Corporation, Littleton, Massachusetts; The Liberian Institute for Biomedical Research, Monrovia, Liberia; Vector Biology Research Program Naval Medical Research Unit No. 3, Cairo, Egypt; Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, Burkina Faso

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Joseph W. Diclaro II Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado; Special Programs, Infoscitex Corporation, Littleton, Massachusetts; The Liberian Institute for Biomedical Research, Monrovia, Liberia; Vector Biology Research Program Naval Medical Research Unit No. 3, Cairo, Egypt; Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, Burkina Faso

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Roch K. Dabiré Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado; Special Programs, Infoscitex Corporation, Littleton, Massachusetts; The Liberian Institute for Biomedical Research, Monrovia, Liberia; Vector Biology Research Program Naval Medical Research Unit No. 3, Cairo, Egypt; Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, Burkina Faso

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Brian D. Foy Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado; Department of Biomedical Sciences, Colorado State University, Fort Collins, Colorado; Special Programs, Infoscitex Corporation, Littleton, Massachusetts; The Liberian Institute for Biomedical Research, Monrovia, Liberia; Vector Biology Research Program Naval Medical Research Unit No. 3, Cairo, Egypt; Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, Burkina Faso

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In this study, we characterize the ability of the previously described Infoscitex tent (IST) to capture mosquitoes in comparison to either the Centers for Disease Control Light Trap hung next to individuals under a bed net (LTC) or to human landing catches (HLC). In Senegal, the IST caught 6.14 times the number of Anopheles gambiae sensu lato (s.l.), and 8.78 times the Culex group V mosquitoes as LTC. In one of two locations in Burkina Faso, the IST caught An. gambiae at a rate not significantly different than HLC. Of importance, 9.1–36.1% of HLC caught An. gambiae were blood fed, mostly with fresh blood, suggesting they fed upon the collector, whereas only 0.5–5.0% from the IST had partial or old blood. The IST also caught outdoor biting species in proportions comparable to HLC. The results show this tent provides a safer and effective alternative to the skill-dependent, risky, and laborious HLC method.

Author Notes

* Address correspondence to Benjamin J. Krajacich, Colorado State University, 1692 Campus Delivery, Fort Collins, CO 80523-1692. E-mail: benjamin.krajacich@colostate.edu

Financial support: Financial support for this project was through a Small Business Innovation Research Grant W81XWH-10-C-0214 from the U.S. Department of Defense, and research in Senegal, Liberia, and Burkina Faso was partially funded by grant 1R01AI094349-01A1 from the U.S. National Institutes of Health.

Authors' addresses: Benjamin J. Krajacich, Haoues Alout, Nathan D. Grubaugh, Jacob I. Meyers, Doug E. Brackney, Timothy A. Burton, Jonathan A. Seaman, and Brian D. Foy, Colorado State University, Fort Collins, CO, E-mails: benjamin.krajacich@colostate.edu, haoues.alout@colostate.edu, nathan.grubaugh@colostate.edu, Jacob.I.Meyers@gmail.com, Doug.Brackney@colostate.edu, timaburton@gmail.com, jonathan.seaman@rams.colostate.edu, and brian.foy@colostate.edu. Jeremiah R. Slade, Robert F. Mulligan, and Brendan LaBrecque, Infoscitex Corporation, Littleton, MA, E-mails: jslade@infoscitex.com, rfm3@email.com, and labrecque.b@gmail.com. Lawrence S. Fakoli III and Fatorma K. Bolay, Liberian Institute for Biomedical Research (LIBR), Charlesville, Margibi County, Liberia, E-mails: lawfako2008@yahoo.com and director.libr@gmail.com. Joseph W. Diclaro II, Vector Biology Research Program, Naval Medical Research Unit No. 3 Cairo Egypt, E-mail: joseph.diclaro@med.navy.mil. Roch K. Dabiré, Institut de Recherche en Sciences de la Santé (IRSS), Bobo Dioulasso, Burkina Faso, E-mail: dabire_roch@hotmail.com.

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