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Reference Manager
BibTeX
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-
1.
Simmons CP, Farrar JJ, Nguyen vV, Wills B, 2012. Dengue. N Engl J Med 366: 1423–1432.
-
2.
World Health Organization, 2009. Dengue: Guidelines for Treatment, Prevention and Control. Geneva: World Health Organization.
-
3.
Andries AC, Duong V, Ngan C, Ong S, Huy R, Sroin KK, Te V, Y B, Try PL, Buchy P, 2012. Field evaluation and impact on clinical management of a rapid diagnostic kit that detects dengue NS1, IgM and IgG. PLoS Negl Trop Dis 6: e1993.
-
4.
Gan VC, Tan KL, Lye DC, Pok KY, Mok SQ, Chua RC, Leo YS, Ng LC, 2014. Diagnosing dengue at point-of-care: utility of a rapid combined diagnostic kit in Singapore. PLoS ONE 9: e90037.
-
5.
Blacksell SD, Jarman RG, Bailey MS, Tanganuchitcharnchai A, Jenjaroen K, Gibbons RV, Paris DH, Premaratna R, de Silva HJ, Lalloo DG, Day NP, 2011. Evaluation of six commercial point-of-care tests for diagnosis of acute dengue infections: the need for combining NS1 antigen and IgM/IgG antibody detection to achieve acceptable levels of accuracy. Clin Vaccine Immunol 18: 2095–2101.
-
6.
Lai YL, Chung YK, Tan HC, Yap HF, Yap G, Ooi EE, Ng LC, 2007. Cost-effective real-time PCR (RT-PCR) to screen for dengue virus followed by rapid single-tube multiplex RT-PCR for serotyping of the virus. J Clin Microbiol 45: 935–941.
-
7.
Santiago GA, Vergne E, Quiles Y, Cosme J, Vazquez J, Medina JF, Colón C, Margolis H, Muñoz-Jordán JL, 2013. Analytical and clinical performance of the CDC real time RT-PCR assay for detection and typing of dengue virus. PLoS Negl Trop Dis 7: e2311.
-
8.
Singapore National Environmental Agency. Campaign Against Dengue. Available at: http://dengue.gov.sg/. Accessed March 4, 2014.
-
9.
Alere, 2014. SD Bioline Dengue Duo NS1 Ag + Ab Combo. Available at: http://www.alere.com/ww/en/product-details/sd-bioline-dengue-duo-ns1-ag—ab-combo.html. Accessed March 4, 2014.
-
10.
Phuong HL, Thai KT, Nga TT, Giao PT, Hung le Q, Binh TQ, Nam NV, Groen J, de Vries PJ, 2009. Detection of dengue nonstructural 1 (NS1) protein in Vietnamese patients with fever. Diagn Microbiol Infect Dis 63: 372–378.
-
11.
Wang SM, Sekaran SD, 2010. Evaluation of a commercial SD dengue virus NS1 antigen capture enzyme-linked immunosorbent assay kit for early diagnosis of dengue virus infection. J Clin Microbiol 48: 2793–2797.
-
12.
Pok KY, Lai YL, Ang J, Ng LC, 2010. Evaluation of nonstructural 1 antigen assays for the diagnosis and surveillance of dengue in Singapore. Vector Borne Zoonotic Dis 10: 1009–1016.
-
13.
Colombo TE, Vedovello D, Araki CS, Cogo-Moreira H, dos Santos IN, Reis AF, Costa FR, Cruz LE, Casagrande L, Regatieri LJ, Junior JF, Bronzoni RV, Schmidt DJ, Nogueira ML, 2013. Dengue-4 false negative results by Panbio® dengue early ELISA assay in Brazil. J Clin Virol 58: 710–712.
-
14.
Felix AC, Romano CM, Centrone C de C, Rodrigues CL, Villas-Boas L, Araújo ES, de Matos AM, Carvalho KI, Martelli CM, Kallas EG, Pannuti CS, Levi JE, 2012. Low sensitivity of NS1 protein tests evidenced during a dengue type 2 virus outbreak in Santos, Brazil in 2010. Clin Vaccine Immunol 19: 1972–1976.
-
15.
Chuansumrit A, Chaiyaratana W, Pongthanapisith V, Tangnararatchakit K, Lertwongrath S, Yoksan S, 2008. The use of dengue nonstructural protein 1 antigen for the early diagnosis during the febrile stage in patients with dengue infection. Pediatr Infect Dis J 27: 43–48.
-
16.
Fry SR, Meyer M, Semple MG, Simmons CP, Sekaran SD, Huang JX, McElnea C, Huang CY, Valks A, Young PR, Cooper MA, 2011. The diagnostic sensitivity of dengue rapid test assays is significantly enhanced by using a combined antigen and antibody testing approach. PLoS Negl Trop Dis 5: e1199.
-
17.
Rueda E, Mendez A, Gonzalez G, 2002. Haemophagocytic syndrome associated with dengue hemorrhagic fever. Biomedica 22: 160–166.
-
18.
Ramanathan M, Duraisamy G, 1991. Haemophagocytosis in dengue haemorrhagic fever: a case report. Ann Acad Med Singapore 20: 803–804.
-
19.
Muller DA, Young PR, 2013. The flavivirus NS1 protein: molecular and structural biology, immunology, role in pathogenesis and application as a diagnostic marker. Antiviral Res 98: 192–208.
-
20.
Osorio L, Ramirez M, Bonelo A, Villar LA, Parra B, 2010. Comparison of the diagnostic accuracy of commercial NS1-based diagnostic tests for early dengue infection. Virol J 7: 361.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 868 | 680 | 165 |
| Full Text Views | 474 | 11 | 1 |
| PDF Downloads | 210 | 14 | 2 |
Two Cases of False-Positive Dengue Non-Structural Protein 1 (NS1) Antigen in Patients with Hematological Malignancies and a Review of the Literature on the Use of NS1 for the Detection of Dengue Infection
Shimin J. Chung
Shimin J. Chung
Department of Infectious Diseases, Singapore General Hospital, Singapore; Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore; National Public Health Laboratory, Ministry of Health, Singapore; Communicable Diseases Centre, Institute of Infectious Diseases and Epidemiology, Tan Tock Seng Hospital, Singapore; Yong Loo Lin School of Medicine, National University, Singapore; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore
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Prabha U. Krishnan
Prabha U. Krishnan
Department of Infectious Diseases, Singapore General Hospital, Singapore; Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore; National Public Health Laboratory, Ministry of Health, Singapore; Communicable Diseases Centre, Institute of Infectious Diseases and Epidemiology, Tan Tock Seng Hospital, Singapore; Yong Loo Lin School of Medicine, National University, Singapore; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore
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Yee Sin Leo
Yee Sin Leo
Department of Infectious Diseases, Singapore General Hospital, Singapore; Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore; National Public Health Laboratory, Ministry of Health, Singapore; Communicable Diseases Centre, Institute of Infectious Diseases and Epidemiology, Tan Tock Seng Hospital, Singapore; Yong Loo Lin School of Medicine, National University, Singapore; Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore
Search for other papers by Yee Sin Leo in
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Early diagnosis of dengue has been made easier in recent years owing to the advancement in diagnostic technologies. The rapid non-structural protein 1 (NS1) test strip is widely used in many developed and developing regions at risk of dengue. Despite the relatively high specificity of this test, we recently encountered two cases of false-positive dengue NS1 antigen in patients with underlying hematological malignancies. We reviewed the literature for causes of false-positive dengue NS1.
Author Notes
Authors' addresses: Shimin J. Chung, Prabha U. Krishnan, and Yee Sin Leo, The Academia, Singapore General Hospital, Department of Laboratory Medicine, Tan Tock Seng Hospital and Communicable Disease Centre, Singapore, E-mails: jasmine.chung.s.m@sgh.com.sg, prabha_krishnan@ttsh.com.sg, and yee_sin_leo@ttsh.com.sg.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Simmons CP, Farrar JJ, Nguyen vV, Wills B, 2012. Dengue. N Engl J Med 366: 1423–1432.
-
2.
World Health Organization, 2009. Dengue: Guidelines for Treatment, Prevention and Control. Geneva: World Health Organization.
-
3.
Andries AC, Duong V, Ngan C, Ong S, Huy R, Sroin KK, Te V, Y B, Try PL, Buchy P, 2012. Field evaluation and impact on clinical management of a rapid diagnostic kit that detects dengue NS1, IgM and IgG. PLoS Negl Trop Dis 6: e1993.
-
4.
Gan VC, Tan KL, Lye DC, Pok KY, Mok SQ, Chua RC, Leo YS, Ng LC, 2014. Diagnosing dengue at point-of-care: utility of a rapid combined diagnostic kit in Singapore. PLoS ONE 9: e90037.
-
5.
Blacksell SD, Jarman RG, Bailey MS, Tanganuchitcharnchai A, Jenjaroen K, Gibbons RV, Paris DH, Premaratna R, de Silva HJ, Lalloo DG, Day NP, 2011. Evaluation of six commercial point-of-care tests for diagnosis of acute dengue infections: the need for combining NS1 antigen and IgM/IgG antibody detection to achieve acceptable levels of accuracy. Clin Vaccine Immunol 18: 2095–2101.
-
6.
Lai YL, Chung YK, Tan HC, Yap HF, Yap G, Ooi EE, Ng LC, 2007. Cost-effective real-time PCR (RT-PCR) to screen for dengue virus followed by rapid single-tube multiplex RT-PCR for serotyping of the virus. J Clin Microbiol 45: 935–941.
-
7.
Santiago GA, Vergne E, Quiles Y, Cosme J, Vazquez J, Medina JF, Colón C, Margolis H, Muñoz-Jordán JL, 2013. Analytical and clinical performance of the CDC real time RT-PCR assay for detection and typing of dengue virus. PLoS Negl Trop Dis 7: e2311.
-
8.
Singapore National Environmental Agency. Campaign Against Dengue. Available at: http://dengue.gov.sg/. Accessed March 4, 2014.
-
9.
Alere, 2014. SD Bioline Dengue Duo NS1 Ag + Ab Combo. Available at: http://www.alere.com/ww/en/product-details/sd-bioline-dengue-duo-ns1-ag—ab-combo.html. Accessed March 4, 2014.
-
10.
Phuong HL, Thai KT, Nga TT, Giao PT, Hung le Q, Binh TQ, Nam NV, Groen J, de Vries PJ, 2009. Detection of dengue nonstructural 1 (NS1) protein in Vietnamese patients with fever. Diagn Microbiol Infect Dis 63: 372–378.
-
11.
Wang SM, Sekaran SD, 2010. Evaluation of a commercial SD dengue virus NS1 antigen capture enzyme-linked immunosorbent assay kit for early diagnosis of dengue virus infection. J Clin Microbiol 48: 2793–2797.
-
12.
Pok KY, Lai YL, Ang J, Ng LC, 2010. Evaluation of nonstructural 1 antigen assays for the diagnosis and surveillance of dengue in Singapore. Vector Borne Zoonotic Dis 10: 1009–1016.
-
13.
Colombo TE, Vedovello D, Araki CS, Cogo-Moreira H, dos Santos IN, Reis AF, Costa FR, Cruz LE, Casagrande L, Regatieri LJ, Junior JF, Bronzoni RV, Schmidt DJ, Nogueira ML, 2013. Dengue-4 false negative results by Panbio® dengue early ELISA assay in Brazil. J Clin Virol 58: 710–712.
-
14.
Felix AC, Romano CM, Centrone C de C, Rodrigues CL, Villas-Boas L, Araújo ES, de Matos AM, Carvalho KI, Martelli CM, Kallas EG, Pannuti CS, Levi JE, 2012. Low sensitivity of NS1 protein tests evidenced during a dengue type 2 virus outbreak in Santos, Brazil in 2010. Clin Vaccine Immunol 19: 1972–1976.
-
15.
Chuansumrit A, Chaiyaratana W, Pongthanapisith V, Tangnararatchakit K, Lertwongrath S, Yoksan S, 2008. The use of dengue nonstructural protein 1 antigen for the early diagnosis during the febrile stage in patients with dengue infection. Pediatr Infect Dis J 27: 43–48.
-
16.
Fry SR, Meyer M, Semple MG, Simmons CP, Sekaran SD, Huang JX, McElnea C, Huang CY, Valks A, Young PR, Cooper MA, 2011. The diagnostic sensitivity of dengue rapid test assays is significantly enhanced by using a combined antigen and antibody testing approach. PLoS Negl Trop Dis 5: e1199.
-
17.
Rueda E, Mendez A, Gonzalez G, 2002. Haemophagocytic syndrome associated with dengue hemorrhagic fever. Biomedica 22: 160–166.
-
18.
Ramanathan M, Duraisamy G, 1991. Haemophagocytosis in dengue haemorrhagic fever: a case report. Ann Acad Med Singapore 20: 803–804.
-
19.
Muller DA, Young PR, 2013. The flavivirus NS1 protein: molecular and structural biology, immunology, role in pathogenesis and application as a diagnostic marker. Antiviral Res 98: 192–208.
-
20.
Osorio L, Ramirez M, Bonelo A, Villar LA, Parra B, 2010. Comparison of the diagnostic accuracy of commercial NS1-based diagnostic tests for early dengue infection. Virol J 7: 361.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 868 | 680 | 165 |
| Full Text Views | 474 | 11 | 1 |
| PDF Downloads | 210 | 14 | 2 |