A New Population and Habitat for Neotricula aperta in the Mekong River of Northeastern Thailand: A DNA Sequence-Based Phylogenetic Assessment Confirms Identifications and Interpopulation Relationships

Yanin Limpanont Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand; School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, Thailand

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Phiraphol Chusongsang Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand; School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, Thailand

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Yupa Chusongsang Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand; School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, Thailand

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Jareemate Limsomboon Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand; School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, Thailand

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Oranuch Sanpool Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand; School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, Thailand

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Worasak Kaewkong Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand; School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, Thailand

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Pewpan M. Intapan Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand; School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, Thailand

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Penchom Janwan Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand; School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, Thailand

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Lakkhana Sadaow Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand; School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, Thailand

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Wanchai Maleewong Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand; School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, Thailand

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DOI:
https://doi.org/10.4269/ajtmh.14-0467
Volume/Issue:
Volume 92: Issue 2
Page(s):
336–339
Restricted access
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Neotricula aperta (Gastropoda: Pomatiopsidae), the snail intermediate host of Schistosoma mekongi, is found in Cambodia, Laos, and Thailand. We update information on the distribution of this species in the Mekong River and its tributary, the Mun River, in Thailand. DNA sequences of a portion of the mitochondrial cytochrome c oxidase subunit 1 were obtained from N. aperta collected from different locations and used to confirm species and strain identities. Specimens of the β-strain were found in the Mun River, whereas specimens of the γ-strain were found in the Mekong River. The γ-strain (with molecular confirmation of identity) is newly reported from Nong Khai Province, where it occurred in a habitat novel for this species: under paving slabs instead of under natural bed rocks, where agal aufwuchs is extensively located on the islet in the middle of the Mekong River. The new location is approximate 400 km upstream from the nearest previously known site for this species.

Author Notes

* Address correspondence to Wanchai Maleewong, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. E-mail: wanch_ma@kku.ac.th

Financial support: This research was funded by Thailand Research Fund (TRF) Grant MRG5680085 (to Y.L.). O.S., W.K., P.M.I., P.J., L.S., and W.M. were supported by a TRF Senior Research Scholar Grant, TRF Grant RTA5580004, and Faculty of Medicine Grant, Khon Kaen University Grant TR57201.

Authors' addresses: Yanin Limpanont, Phiraphol Chusongsang, Yupa Chusongsang, and Jareemate Limsomboon, Southeast Asian Center for Medical Malacology, Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, E-mails: yanin.lim@mahidol.ac.th, phiraphol.chu@mahidol.ac.th, yupa.chu@mahidol.ac.th, and jareemate.lim@mahidol.ac.th. Oranuch Sanpool, Pewpan M. Intapan, Lakkhana Sadaow, and Wanchai Maleewong, Department of Parasitology, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand, E-mails: sanpoolor@yahoo.com, pewpan@kku.ac.th, sadaow1986@hotmail.com, and wanch_ma@kku.ac.th. Worasak Kaewkong, Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand, E-mail: worasak_bc@hotmail.com. Penchom Janwan, Department of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, Thailand, E-mail: pair_wu@yahoo.com.

Copyright:
© The American Society of Tropical Medicine and Hygiene 2015
Received:
27 Jul 2014
|
Accepted:
09 Nov 2014
|
Published Online:
04 Feb 2015
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Online ISSN:
1476-1645
  • 1.

    Attwood SW, Upatham ES, 2012. Observations on Neotricula aperta (Gastropoda: Pomatiopsidae) population densities in Thailand and central Laos: implications for the spread of Mekong schistosomiasis. Parasit Vectors 5: 126.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 2.

    Urbani C, Sinoun M, Socheat D, Pholsena K, Strandgaard H, Odermatt P, Hatz C, 2002. Epidemiology and control of mekongi schistosomiasis. Acta Trop 82: 157–168.

  • 3.

    Davis GM, Kitikoon V, Temcharoen P, 1976. Monograph on “Lithoglyphopsis” aperta, the snail host of Mekong River Schistosomiasis. Malacologia 15: 241–287.

  • 4.

    Tropmed Technical Group, 1986. Snails of medical importance in Southeast Asia. Southeast Asian J Trop Med Public Health 17: 282–322.

  • 5.

    Attwood SW, Fatih FA, Campbell I, Upatham ES, 2008. The distribution of Mekong schistosomiasis, past and future: preliminary indications from an analysis of genetic variation in the intermediate host. Parasitol Int 57: 256–270.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 6.

    Attwood SW, 2010. Studies on the parasitology, phylogeography and the evolution of host-parasite interactions for the snail intermediate hosts of medically important trematode genera in Southeast Asia. Adv Parasitol 73: 405–440.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 7.

    Attwood SW, Ambu S, Meng X-H, Upatham ES, Xu F-S, Southgate VR, 2003. The Phylogenetics of Triculine snails (Rissooidea: Pomatiopsidae) from South-east Asia and Southern China: historical biogeography and the transmission of human Schistosomiasis. J Molluscan Stud 69: 263–271.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 8.

    Staub KC, Woodruff DS, Upatham ES, Viyanant V, 1990. Genetic variation in Neotricula aperta, the intermediate snail host of Schistosoma mekongi: allozyme differences reveal a group of sibling species. Am Malacol Bull 7: 93–103.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 9.

    Attwood SW, Kitikoon V, Southgate VR, 1998. Neotricula aperta (Gastropoda: Pomatiopsidae), the intermediate host of Schistosoma mekongi: allozyme variation and relationships between Khmer, Lao and Thai populations. J Zool (Lond) 246: 309–324.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 10.

    Attwood SW, 1999. Genetic variation in Neotricula aperta, the snail intermediate host of Schistosoma mekongi in the lower Mekong Basin. J Zool (Lond) 249: 153–164.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 11.

    Attwood SW, Johnston DA, 2001. Nucleotide sequence differences reveal genetic variation on Neotricula aperta (Gastropoda: Pomatiopsidae), the snail host of Schistosomiasis in the lower Mekong Basin. Biol J Linn Soc Lond 73: 23–41.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 12.

    Attwood SW, Upatham ES, Zhang Y-P, Yang ZQ, Southgate VR, 2004. A DNA-sequence based phylogeny for triculine snails (Gastropoda: Pomatiopsidae: Triculinae), intermediate hosts for Schistosoma (Trematode: Digenea): phylogeography and the origin of Neotricula. J Zool (Lond) 262: 47–56.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 13.

    Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S, 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739.

    • PubMed
    • Search Google Scholar
    • Export Citation
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