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Seroreactivity to a Large Panel of Field-Derived Plasmodium falciparum Apical Membrane Antigen 1 and Merozoite Surface Protein 1 Variants Reflects Seasonal and Lifetime Acquired Responses to Malaria

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  • Howard Hughes Medical Institute/Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland; Division of Infectious Diseases, Department of Medicine, University of California, Irvine, California; Laboratory of Immunogenetics, National Institutes of Health, Bethesda, Maryland; Malaria Research and Training Center, Department of Epidemiology of Parasitic Diseases, University of Sciences, Techniques and Technology, Bamako, Mali

Parasite antigen diversity poses an obstacle to developing an effective malaria vaccine. A protein microarray containing Plasmodium falciparum apical membrane antigen 1 (AMA1, n = 57) and merozoite surface protein 1 19-kD (MSP119, n = 10) variants prevalent at a malaria vaccine testing site in Bandiagara, Mali, was used to assess changes in seroreactivity caused by seasonal and lifetime exposure to malaria. Malian adults had significantly higher magnitude and breadth of seroreactivity to variants of both antigens than did Malian children. Seroreactivity increased over the course of the malaria season in children and adults, but the difference was more dramatic in children. These results help to validate diversity-covering protein microarrays as a promising tool for measuring the breadth of antibody responses to highly variant proteins, and demonstrate the potential of this new tool to help guide the development of malaria vaccines with strain-transcending efficacy.

Author Notes

* Address correspondence to Christopher V. Plowe, Howard Hughes Medical Institute/Center for Vaccine Development, University of Maryland School of Medicine, 685 West Baltimore Street, HSF1 480, Baltimore, MD 21201. E-mail: cplowe@medicine.umaryland.edu

Authors' addresses: Jason A. Bailey, Mark A. Travassos, Amed Ouattara, Matthew B. Laurens, Shannon L. Takala-Harrison, Kirsten E. Lyke, and Christopher V. Plowe, Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD, E-mails: jbail006@umaryland.edu, aouattara@medicine.umaryland.edu, mlaurens@medicine.umaryland.edu, stakala@medicine.umaryland.edu, klyke@medicine.umaryland.edu, and cplowe@medicine.umaryland.edu. Jozelyn Pablo, Algis Jasinskas, Rie Nakajima-Sasaki, and Philip L. Felgner, Division of Infectious Diseases, University of California, Irvine, CA, E-mails: jozelynp@gmail.com, ajasinsk@uci.edu, and rie3@uci.edu. Amadou Niangaly, Drissa Coulibaly, and Mahamadou A. Thera, Faculty of Medicine, Malaria Research and Training Center Bamako, University of Bamako, Bamako, Mali, and Malaria Research and Training Center, Mali University of Science, Techniques, and Technology of Bamako, Bamako, Mali, E-mails: niangaly@icermali.org, coulibalyd@icermali.org, and mthera@icermali.org. Jeff Skinner, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health Rockville, MD, E-mail: skinnerj@niaid.nih.gov. Bourema Kouriba and Ogobara K. Doumbo, Malaria Research and Training Center, University of Science, Techniques, and Technology of Bamako, Bamako, Mali, E-mails: kouriba@icermali.org and okd@icermali.org.

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