Yanagida T, Mohammadzadeh T, Kamhawi S, Nakao M, Sadjjadi SM, Hijjawi N, Abdel-Hafez SK, Sako Y, Okamoto M, Ito A, 2012. Genetic polymorphisms of Echinococcus granulosus sensu stricto in the Middle East. Parasitol Int 61: 599–603.
Ahn KS, Hong ST, Kang YN, Kwon JH, Kim MJ, Park TJ, Kim YH, Lim TJ, Kang KJ, 2012. An imported case of cystic echinococcosis in the liver. Korean J Parasitol 50: 357–360.
Ahmadi NA, Meshkehkar M, 2011. An abattoir-based study on the prevalence and economic losses due to cystic echinococcosis in slaughtered herbivores in Ahwaz, south-western Iran. J Helminthol 85: 33–39.
Metanat M, Sharifi-mood B, Sandoghi M, Alavi-Naini R, 2008. Osseous hydatid disease. Iran J Parasitol 3: 60–64.
Babba H, Messedi A, Masmoudi S, Zribi M, Grillot R, Ambriosethomas P, Beyrouti I, Sahnoun Y, 1994. Diagnosis of human hydatidosis - comparison between imagery and 6 serologic techniques. Am J Trop Med Hyg 50: 64–68.
Mariconti M, Bazzocchi C, Tamarozzi F, Meroni V, Genco F, Maserati R, Brunetti E, 2014. Immunoblotting with human native antigen shows stage-related sensitivity in the serodiagnosis of hepatic cystic echinococcosis. Am J Trop Med Hyg 90: 75–79.
Ortona E, Rigano R, Buttari B, Delunardo F, Ioppolo S, Margutti P, Profumo E, Teggi A, Vaccari S, Siracusano A, 2003. An update on immunodiagnosis of cystic echinococcosis. Acta Trop 85: 165–171.
Hewitson JP, Grainger JR, Maizels RM, 2009. Helminth immunoregulation: the role of parasite secreted proteins in modulating host immunity. Mol Biochem Parasitol 167: 1–11.
Zhang W, McManus D, 2013. Advances in the immunology and diagnosis of cystic echinococcosis. Filippou D, ed. Advances in Hydatid Disease, Diagnosis and Treatment. Nicosia, Cyprus: Broken Hill, 147–166.
Craig PS, Rogan MT, Campos-Ponce M, 2003. Echinococcosis: disease, detection and transmission. Parasitology 127: S5–S20.
Mohammadzadeh T, Sako Y, Sadjjadi SM, Sarkari B, Ito A, 2012. Comparison of the usefulness of hydatid cyst fluid, native antigen B and recombinant antigen B8/1 for serological diagnosis of cystic echinococcosis. Trans R Soc Trop Med Hyg 106: 371–375.
Baveja UK, Basak S, Thusoo TK, 1997. Immunodiagnosis of human hydatid disease. J Commun Dis 29: 313–319.
Di Felice G, Pini C, Afferni C, Vicari G, 1986. Purification and partial characterization of the major antigen of Echinococcus granulosus (antigen 5) with monoclonal antibodies. Mol Biochem Parasitol 20: 133–142.
Sarkari B, Sadjjadi SM, Abidi H, Izadpanah A, Kazemian S, Rafati A, 2007. Application of Western blotting using native antigen B for serodiagnosis of human cystic echinococcosis. Iran J Parasitol 2: 7–12.
Mamuti W, Sako Y, Nakao M, Xiao N, Nakaya K, Ishikawa Y, Yamasaki H, Lightowlers MW, Ito A, 2006. Recent advances in characterization of Echinococcus antigen B. Parasitol Int 55 (Suppl): S57–S62.
Haag KL, Zaha A, Araujo AM, Gottstein B, 1997. Reduced genetic variability within coding and non-coding regions of the Echinococcus multilocularis genome. Parasitology 115: 521–529.
Murray CK, Gasser RA, Magill AJ, Miller RS, 2008. Update on rapid diagnostic testing for malaria. Clin Microbiol Rev 21: 97–110.
van Doorn HR, Koelewijn R, Hofwegen H, Gilis H, Wetsteyn JCFM, Wismans PJ, Sarfati C, Vervoort T, van Gool T, 2007. Use of enzyme-linked immunosorbent assay and dipstick assay for detection of Strongyloides stercoralis infection in humans. J Clin Microbiol 45: 438–442.
Rahmah N, Shenoy RK, Nutman TB, Weiss N, Gilmour K, Maizels RM, Yazdanbakhsh M, Sartono E, 2003. Multicentre laboratory evaluation of Brugia Rapid dipstick test for detection of brugian filariasis. Trop Med Int Health 8: 895–900.
Xu J, Feng T, Lin DD, Wang QZ, Tang L, Wu XH, Guo JG, Peeling RW, Zhou XN, 2011. Performance of a dipstick dye immunoassay for rapid screening of Schistosoma japonicum infection in areas of low endemicity. Parasite Vector 4: e87.
Oriol R, Williams JF, Pérez EM, Oriol C, 1971. Purification of lipoprotein antigens of Echinococcus granulosus from sheep hydatid fluid. Am J Trop Med Hyg 20: 569–574.
Sadjjadi SM, Sedaghat F, Hosseini SV, Sarkari B, 2009. Serum antigen and antibody detection in echinococcosis: application in serodiagnosis of human hydatidosis. Korean J Parasitol 47: 153–157.
Bradford MM, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254.
Khalilpour A, Santhanam A, Wei LC, Saadatnia G, Velusamy N, Osman S, Ahmad MM, Noordin R, 2013. Antigenic proteins of Helicobacter pylori of potential diagnostic value. Asian Pac J Cancer Prev 14: 1635–1642.
Saidin S, Yunus MH, Zakaria ND, Razak KA, Huat LB, Othman N, Noordin R, 2014. Production of recombinant Entamoeba histolytica pyruvate phosphate dikinase and its application in a lateral flow dipstick test for amoebic liver abscess. BMC Infect Dis 14: 182.
O'Farrell B, 2009. Evolution in lateral flow-based immunoassay systems. Wong R, Tse H, eds. Lateral Flow Immunoassay. New York: Humana Press, 1–33.
Makhsin SR, Razak KA, Noordin R, Zakaria ND, Chun TS, 2012. The effects of size and synthesis methods of gold nanoparticle-conjugated M alpha HIgG(4) for use in an immunochromatographic strip test to detect brugian filariasis. Nanotechnology 23: e495719.
Eckert J, Deplazes P, 2004. Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin Microbiol Rev 17: 107–135.
Gomara MJ, Haro I, 2007. Synthetic peptides for the immunodiagnosis of human diseases. Curr Med Chem 14: 531–546.
Hernandez-Gonzalez A, Santivanez S, Garcia HH, Rodriguez S, Munoz S, Ramos G, Orduna A, Siles-Lucas M, 2012. Improved serodiagnosis of cystic echinococcosis using the new recombinant 2B2t antigen. PLoS Negl Trop Dis 6: e1714.
Carmena D, Benito A, Eraso E, 2006. Antigens for the immunodiagnosis of Echinococcus granulosus infection: an update. Acta Trop 98: 74–86.
Lorenzo C, Last JA, González-Sapienza GG, 2005. The immunogenicity of Echinococcus granulosus antigen 5 is determined by its post-translational modifications. Parasitology 131: 669–677.
Virginio VG, Hernandez A, Rott MB, Monteiro KM, Zandonai AF, Nieto A, Zaha A, Ferreira HB, 2003. A set of recombinant antigens from Echinococcus granulosus with potential for use in the immunodiagnosis of human cystic hydatid disease. Clin Exp Immunol 132: 309–315.
Hujakka H, Koistinen V, Kuronen I, Eerikainen P, Parviainen M, Lundkvist A, Vaheri A, Vapalahti O, Narvanen A, 2003. Diagnostic rapid tests for acute hantavirus infections: specific tests for Hantaan, Dobrava and Puumala viruses versus a hantavirus combination test. J Virol Methods 108: 117–122.
Olsen DA, 1995. Immunoassay Method for the Rapid Identification of Detergent Treated Antigens. U.S. Patent No. 5393658. Washington, DC.
Al-Sherbiny MM, Farrag AAMMK, Fayad MH, Makled MK, Tawfeek GM, Ali NMS, 2004. Application and assessment of a dipstick assay in the diagnosis of hydatidosis and trichinosis. Parasitol Res 93: 87–95.
Feng XH, Wen H, Zhang ZX, Chen XH, Ma XD, Zhang JP, Qi XW, Bradshaw H, Vuitton D, Craig PS, 2010. Dot immunogold filtration assay (DIGFA) with multiple native antigens for rapid serodiagnosis of human cystic and alveolar echinococcosis. Acta Trop 113: 114–120.
Sbihi Y, Gil JR, Alvarez PA, Orduña A, RodrÃguez-Torres A, Osuna A, 2003. Development of a dipstick dye immunoassay for diagnosing hydatidosis. J Clin Lab Anal 17: 219–22.
Hernandez Gonzalez A, 2012. Validación de un nuevo antÃgeno recombinante para el diagnóstico y seguimiento de pacientes con hidatidosis, y su aplicación a un test comercial. Available at: http://gredos.usal.es/jspui/handle/10366/121370. Accessed March 19, 2014.
McVie A, Ersfeld K, Rogan MT, Craig PS, 1997. Expression and immunological characterisation of Echinococcus granulosus recombinant antigen B for IgG4 subclass detection in human cystic echinococcosis. Acta Trop 67: 19–35.
Daeki AO, Craig PS, Shambesh MK, 2000. IgG-subclass antibody responses and the natural history of hepatic cystic echinococcosis in asymptomatic patients. Ann Trop Med Parasitol 94: 319–328.
Rigano R, Profumo E, Ioppolo S, Notargiacomo S, Ortona E, Teggi A, Siracusano A, 1995. Immunological markers indicating the effectiveness of pharmacological treatment in human hydatid disease. Clin Exp Immunol 102: 281–285.
Guerri ML, Davila M, Rodriguez M, Nieto FJ, Ladron de Guevara C, 2000. Utility of IgG subclasses in the diagnosis and follow up of hydatidosis. Enferm Infecc Microbiol Clin 18: 262–266.
Zhang W, Li J, McManus DP, 2003. Concepts in immunology and diagnosis of hydatid disease. Clin Microbiol Rev 16: 18–36.
Moghadam ZK, Ghaffarifar F, Khalilpour A, Abdul Aziz F, Saadatnia G, Noordin R, 2013. IgG4 detection of Echinococcus granulosus paramyosin is a useful diagnostic test for human hydatidosis. Clin Vaccine Immunol 20: 501–505.
Maddison SE, Slemenda SB, Schantz PM, Fried JA, Wilson M, Tsang VC, 1989. A specific diagnostic antigen of Echinococcus granulosus with an apparent molecular weight of 8 kDA. Am J Trop Med Hyg 40: 377–383.
Ito A, 2002. Serologic and molecular diagnosis of zoonotic larval cestode infections. Parasitol Int 51: 221–235.
Virginio VG, Hernández A, Rott MB, Monteiro KM, Zandonai AF, Nieto A, Zaha A, Ferreira HB, 2003. A set of recombinant antigens from Echinococcus granulosus with potential for use in the immunodiagnosis of human cystic hydatid disease. Clin Exp Immunol 132: 309–315.
Hernández-González A, Muro A, Barrera I, Ramos G, Orduña A, Siles-Lucas M, 2008. Usefulness of four different Echinococcus granulosus recombinant antigens for serodiagnosis of unilocular hydatid disease (UHD) and postsurgical follow-up of patients treated for UHD. Clin Vaccine Immunol 15: 147–153.
Rokni MB, Aminian B, 2006. Evaluation of the Enzyme-linked Immuno-electro Transfer Blot (EITB) technique using hydatid cyst antigens B/5 and total IgG antibodies in laboratory diagnosis of human hydatidosis. Pak J Med Sci 22: 127–131.
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Cystic echinococcosis (CE) caused by infection with Echinococcus granulosus is of major concern for humans in many parts of the world. Antigen B was prepared from E. granulosus hydatid fluid, and Western blots confirmed eight batches showing a band corresponding to the 8-/12-kDa subunit with positive serum and no low-molecular mass band (< 15 kDa) with negative serum. The batches were pooled and used to prepare lateral flow immunoglobulin G4 (IgG4) and IgG dipsticks. Diagnostic sensitivity was determined using serum samples from 21 hydatidosis patients, and diagnostic specificity was established using sera from 17 individuals infected with other parasites and 15 healthy people. IgG4 dipstick had a diagnostic sensitivity of 95% (20 of 21) and a specificity of 100% (32 of 32). The IgG dipstick had a sensitivity of 100% (21 of 21) and a specificity of 87.5% (28 of 32). Thus, both IgG and IgG4 dipsticks had high sensitivities, but IgG4 had greater specificity for the diagnosis of human CE.
Financial support: This study was funded by Malaysian Ministry of Higher Education HiCOE Research Grant 311/CIPPM/4401005 and the Shiraz University of Medical Sciences.
Authors' addresses: Akbar Khalilpour, Zohreh Kazemi Moghadam, Muhammad Hafiznur Yunus, Nor Dyana Zakaria, Sabariah Osman, and Rahmah Noordin, Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia, E-mails: akbarkhalilpour@gmail.com, aram106p@yahoo.com, mhn1016@yahoo.com, nordyana@usm.my, sabariaho@yahoo.com, and rahmah8485@gmail.com. Seyed Mahmoud Sadjjadi, Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran, E-mail: smsadjjadi@sums.ac.ir.
Reprint requests: Rahmah Noordin, Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang 11800, Malaysia, E-mail: rahmah8485@gmail.com; or Seyed Mahmoud Sadjjadi, Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran, E-mail: sadjjadi316@gmail.com.
Yanagida T, Mohammadzadeh T, Kamhawi S, Nakao M, Sadjjadi SM, Hijjawi N, Abdel-Hafez SK, Sako Y, Okamoto M, Ito A, 2012. Genetic polymorphisms of Echinococcus granulosus sensu stricto in the Middle East. Parasitol Int 61: 599–603.
Ahn KS, Hong ST, Kang YN, Kwon JH, Kim MJ, Park TJ, Kim YH, Lim TJ, Kang KJ, 2012. An imported case of cystic echinococcosis in the liver. Korean J Parasitol 50: 357–360.
Ahmadi NA, Meshkehkar M, 2011. An abattoir-based study on the prevalence and economic losses due to cystic echinococcosis in slaughtered herbivores in Ahwaz, south-western Iran. J Helminthol 85: 33–39.
Metanat M, Sharifi-mood B, Sandoghi M, Alavi-Naini R, 2008. Osseous hydatid disease. Iran J Parasitol 3: 60–64.
Babba H, Messedi A, Masmoudi S, Zribi M, Grillot R, Ambriosethomas P, Beyrouti I, Sahnoun Y, 1994. Diagnosis of human hydatidosis - comparison between imagery and 6 serologic techniques. Am J Trop Med Hyg 50: 64–68.
Mariconti M, Bazzocchi C, Tamarozzi F, Meroni V, Genco F, Maserati R, Brunetti E, 2014. Immunoblotting with human native antigen shows stage-related sensitivity in the serodiagnosis of hepatic cystic echinococcosis. Am J Trop Med Hyg 90: 75–79.
Ortona E, Rigano R, Buttari B, Delunardo F, Ioppolo S, Margutti P, Profumo E, Teggi A, Vaccari S, Siracusano A, 2003. An update on immunodiagnosis of cystic echinococcosis. Acta Trop 85: 165–171.
Hewitson JP, Grainger JR, Maizels RM, 2009. Helminth immunoregulation: the role of parasite secreted proteins in modulating host immunity. Mol Biochem Parasitol 167: 1–11.
Zhang W, McManus D, 2013. Advances in the immunology and diagnosis of cystic echinococcosis. Filippou D, ed. Advances in Hydatid Disease, Diagnosis and Treatment. Nicosia, Cyprus: Broken Hill, 147–166.
Craig PS, Rogan MT, Campos-Ponce M, 2003. Echinococcosis: disease, detection and transmission. Parasitology 127: S5–S20.
Mohammadzadeh T, Sako Y, Sadjjadi SM, Sarkari B, Ito A, 2012. Comparison of the usefulness of hydatid cyst fluid, native antigen B and recombinant antigen B8/1 for serological diagnosis of cystic echinococcosis. Trans R Soc Trop Med Hyg 106: 371–375.
Baveja UK, Basak S, Thusoo TK, 1997. Immunodiagnosis of human hydatid disease. J Commun Dis 29: 313–319.
Di Felice G, Pini C, Afferni C, Vicari G, 1986. Purification and partial characterization of the major antigen of Echinococcus granulosus (antigen 5) with monoclonal antibodies. Mol Biochem Parasitol 20: 133–142.
Sarkari B, Sadjjadi SM, Abidi H, Izadpanah A, Kazemian S, Rafati A, 2007. Application of Western blotting using native antigen B for serodiagnosis of human cystic echinococcosis. Iran J Parasitol 2: 7–12.
Mamuti W, Sako Y, Nakao M, Xiao N, Nakaya K, Ishikawa Y, Yamasaki H, Lightowlers MW, Ito A, 2006. Recent advances in characterization of Echinococcus antigen B. Parasitol Int 55 (Suppl): S57–S62.
Haag KL, Zaha A, Araujo AM, Gottstein B, 1997. Reduced genetic variability within coding and non-coding regions of the Echinococcus multilocularis genome. Parasitology 115: 521–529.
Murray CK, Gasser RA, Magill AJ, Miller RS, 2008. Update on rapid diagnostic testing for malaria. Clin Microbiol Rev 21: 97–110.
van Doorn HR, Koelewijn R, Hofwegen H, Gilis H, Wetsteyn JCFM, Wismans PJ, Sarfati C, Vervoort T, van Gool T, 2007. Use of enzyme-linked immunosorbent assay and dipstick assay for detection of Strongyloides stercoralis infection in humans. J Clin Microbiol 45: 438–442.
Rahmah N, Shenoy RK, Nutman TB, Weiss N, Gilmour K, Maizels RM, Yazdanbakhsh M, Sartono E, 2003. Multicentre laboratory evaluation of Brugia Rapid dipstick test for detection of brugian filariasis. Trop Med Int Health 8: 895–900.
Xu J, Feng T, Lin DD, Wang QZ, Tang L, Wu XH, Guo JG, Peeling RW, Zhou XN, 2011. Performance of a dipstick dye immunoassay for rapid screening of Schistosoma japonicum infection in areas of low endemicity. Parasite Vector 4: e87.
Oriol R, Williams JF, Pérez EM, Oriol C, 1971. Purification of lipoprotein antigens of Echinococcus granulosus from sheep hydatid fluid. Am J Trop Med Hyg 20: 569–574.
Sadjjadi SM, Sedaghat F, Hosseini SV, Sarkari B, 2009. Serum antigen and antibody detection in echinococcosis: application in serodiagnosis of human hydatidosis. Korean J Parasitol 47: 153–157.
Bradford MM, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254.
Khalilpour A, Santhanam A, Wei LC, Saadatnia G, Velusamy N, Osman S, Ahmad MM, Noordin R, 2013. Antigenic proteins of Helicobacter pylori of potential diagnostic value. Asian Pac J Cancer Prev 14: 1635–1642.
Saidin S, Yunus MH, Zakaria ND, Razak KA, Huat LB, Othman N, Noordin R, 2014. Production of recombinant Entamoeba histolytica pyruvate phosphate dikinase and its application in a lateral flow dipstick test for amoebic liver abscess. BMC Infect Dis 14: 182.
O'Farrell B, 2009. Evolution in lateral flow-based immunoassay systems. Wong R, Tse H, eds. Lateral Flow Immunoassay. New York: Humana Press, 1–33.
Makhsin SR, Razak KA, Noordin R, Zakaria ND, Chun TS, 2012. The effects of size and synthesis methods of gold nanoparticle-conjugated M alpha HIgG(4) for use in an immunochromatographic strip test to detect brugian filariasis. Nanotechnology 23: e495719.
Eckert J, Deplazes P, 2004. Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin Microbiol Rev 17: 107–135.
Gomara MJ, Haro I, 2007. Synthetic peptides for the immunodiagnosis of human diseases. Curr Med Chem 14: 531–546.
Hernandez-Gonzalez A, Santivanez S, Garcia HH, Rodriguez S, Munoz S, Ramos G, Orduna A, Siles-Lucas M, 2012. Improved serodiagnosis of cystic echinococcosis using the new recombinant 2B2t antigen. PLoS Negl Trop Dis 6: e1714.
Carmena D, Benito A, Eraso E, 2006. Antigens for the immunodiagnosis of Echinococcus granulosus infection: an update. Acta Trop 98: 74–86.
Lorenzo C, Last JA, González-Sapienza GG, 2005. The immunogenicity of Echinococcus granulosus antigen 5 is determined by its post-translational modifications. Parasitology 131: 669–677.
Virginio VG, Hernandez A, Rott MB, Monteiro KM, Zandonai AF, Nieto A, Zaha A, Ferreira HB, 2003. A set of recombinant antigens from Echinococcus granulosus with potential for use in the immunodiagnosis of human cystic hydatid disease. Clin Exp Immunol 132: 309–315.
Hujakka H, Koistinen V, Kuronen I, Eerikainen P, Parviainen M, Lundkvist A, Vaheri A, Vapalahti O, Narvanen A, 2003. Diagnostic rapid tests for acute hantavirus infections: specific tests for Hantaan, Dobrava and Puumala viruses versus a hantavirus combination test. J Virol Methods 108: 117–122.
Olsen DA, 1995. Immunoassay Method for the Rapid Identification of Detergent Treated Antigens. U.S. Patent No. 5393658. Washington, DC.
Al-Sherbiny MM, Farrag AAMMK, Fayad MH, Makled MK, Tawfeek GM, Ali NMS, 2004. Application and assessment of a dipstick assay in the diagnosis of hydatidosis and trichinosis. Parasitol Res 93: 87–95.
Feng XH, Wen H, Zhang ZX, Chen XH, Ma XD, Zhang JP, Qi XW, Bradshaw H, Vuitton D, Craig PS, 2010. Dot immunogold filtration assay (DIGFA) with multiple native antigens for rapid serodiagnosis of human cystic and alveolar echinococcosis. Acta Trop 113: 114–120.
Sbihi Y, Gil JR, Alvarez PA, Orduña A, RodrÃguez-Torres A, Osuna A, 2003. Development of a dipstick dye immunoassay for diagnosing hydatidosis. J Clin Lab Anal 17: 219–22.
Hernandez Gonzalez A, 2012. Validación de un nuevo antÃgeno recombinante para el diagnóstico y seguimiento de pacientes con hidatidosis, y su aplicación a un test comercial. Available at: http://gredos.usal.es/jspui/handle/10366/121370. Accessed March 19, 2014.
McVie A, Ersfeld K, Rogan MT, Craig PS, 1997. Expression and immunological characterisation of Echinococcus granulosus recombinant antigen B for IgG4 subclass detection in human cystic echinococcosis. Acta Trop 67: 19–35.
Daeki AO, Craig PS, Shambesh MK, 2000. IgG-subclass antibody responses and the natural history of hepatic cystic echinococcosis in asymptomatic patients. Ann Trop Med Parasitol 94: 319–328.
Rigano R, Profumo E, Ioppolo S, Notargiacomo S, Ortona E, Teggi A, Siracusano A, 1995. Immunological markers indicating the effectiveness of pharmacological treatment in human hydatid disease. Clin Exp Immunol 102: 281–285.
Guerri ML, Davila M, Rodriguez M, Nieto FJ, Ladron de Guevara C, 2000. Utility of IgG subclasses in the diagnosis and follow up of hydatidosis. Enferm Infecc Microbiol Clin 18: 262–266.
Zhang W, Li J, McManus DP, 2003. Concepts in immunology and diagnosis of hydatid disease. Clin Microbiol Rev 16: 18–36.
Moghadam ZK, Ghaffarifar F, Khalilpour A, Abdul Aziz F, Saadatnia G, Noordin R, 2013. IgG4 detection of Echinococcus granulosus paramyosin is a useful diagnostic test for human hydatidosis. Clin Vaccine Immunol 20: 501–505.
Maddison SE, Slemenda SB, Schantz PM, Fried JA, Wilson M, Tsang VC, 1989. A specific diagnostic antigen of Echinococcus granulosus with an apparent molecular weight of 8 kDA. Am J Trop Med Hyg 40: 377–383.
Ito A, 2002. Serologic and molecular diagnosis of zoonotic larval cestode infections. Parasitol Int 51: 221–235.
Virginio VG, Hernández A, Rott MB, Monteiro KM, Zandonai AF, Nieto A, Zaha A, Ferreira HB, 2003. A set of recombinant antigens from Echinococcus granulosus with potential for use in the immunodiagnosis of human cystic hydatid disease. Clin Exp Immunol 132: 309–315.
Hernández-González A, Muro A, Barrera I, Ramos G, Orduña A, Siles-Lucas M, 2008. Usefulness of four different Echinococcus granulosus recombinant antigens for serodiagnosis of unilocular hydatid disease (UHD) and postsurgical follow-up of patients treated for UHD. Clin Vaccine Immunol 15: 147–153.
Rokni MB, Aminian B, 2006. Evaluation of the Enzyme-linked Immuno-electro Transfer Blot (EITB) technique using hydatid cyst antigens B/5 and total IgG antibodies in laboratory diagnosis of human hydatidosis. Pak J Med Sci 22: 127–131.
Past two years | Past Year | Past 30 Days | |
---|---|---|---|
Abstract Views | 290 | 233 | 57 |
Full Text Views | 417 | 7 | 1 |
PDF Downloads | 92 | 7 | 1 |