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-
1.
Alvar J, Velez ID, Bern C, Herero M, Desjeux P, Cano J, Jannin J, den Boer M; WHO Leishmaniasis Control Team, 2012. Leishmaniasis worldwide and global estimates of its incidence. PLoS ONE 7: e35671.
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2.
Desjeux P, 2004. Leishmaniasis: current situation and new perspectives. Comp Immunol Microbiol Infect Dis 27: 305–318.
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3.
World Health Organization, 2007. Report of the Fifth Consultative Meeting on Leishmania/HIV Coinfection. Available at: http://www.who.int/leishmaniasis/resources/Leishmaniasis_hiv_coinfection5.pdf. Accessed November 1, 2013.
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4.
Ministério da Saúde do Brasil, 2013. Casos de Leishmaniose Tegumentar Americana. Brasil. Grandes Regiões e Unidades Federadas. 1990–2011. Available at: http://portal.saude.gov.br/portal/arquivos/pdf/2012_11_casos_de_lta_entre_1990_e_2011.pdf. Accessed November 1, 2013.
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Levitt J, Bernardo S, Talley Whang T, 2013. How to perform a punch biopsy of the skin—videos in clinical medicine. N Engl J Med 369: e13.
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6.
De Lorenzi D, Mandara MT, Tranquillo M, Baroni M, Gasparinetti N, Gandini G, Masserdotti C, Bonfanti U, Bertolini G, Vian P, Bernardini M, 2006. Squash–prep cytology in the diagnosis of canine and feline nervous system lesions: a study of 42 cases. Vet Clin Pathol 35: 208–214.
-
7.
Mitra S, Kumar M, Sharma V, Mukhopadhyay D, 2010. Squash preparation: a reliable diagnostic tool in the intraoperative diagnosis of central nervous system tumors. J Cytol 27: 81–85.
-
8.
Alves CF, Alves CF, Figueiredo MM, Souza CC, Machado-Coelho GL, Melo MN, Tafuri WL, Raso P, Soares RP, Tafuri WL, 2013. American tegumentary leishmaniasis: effectiveness of an immunohistochemical protocol for the detection of Leishmania in skin. PLoS ONE 8: e63343.
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9.
Berman JD, 1997. Human leishmaniasis: clinical, diagnostic, and chemotherapeutic developments in the last 10 years. Clin Infect Dis 24: 684–703.
-
10.
Weigle KA, de Dávalos M, Heredia P, Molineros R, Saravia NG, D'Alessandro A, 1987. Diagnosis of cutaneous and mucocutaneous leishmaniasis in Colombia: a comparison of seven methods. Am J Trop Med Hyg 36: 489–496.
-
11.
Sousa AQ, Parise ME, Pompeu MM, Coelho Filho JM, Vasconcelos IA, Lima JW, Oliveira EG, Vasconcelos AW, David JR, Maguire JH, 1995. Bubonic leishmaniasis: a common manifestation of Leishmania (Viannia) braziliensis infection in Ceara, Brazil. Am J Trop Med Hyg 53: 380–385.
-
12.
Sousa AQ, Pompeu MML, Frutuoso MS, Moraes EA, Pearson RD, 2012. Reply to Drs. Torres and Suarez. Clin Infect Dis 55: 309–310.
-
13.
Aviles H, Belli A, Armijos R, Monroy FP, Harris E, 1999. PCR detection and identification of Leishmania parasites in clinical specimens in Ecuador: a comparison with classical diagnostic methods. J Parasitol 85: 181–187.
-
14.
Ramirez JR, Agudelo S, Muskus C, 2000. Diagnosis of cutaneous leishmaniasis in Colombia: the sampling site within lesions influences the sensitivity of parasitological diagnosis. J Clin Microbiol 38: 3768–3773.
-
15.
Sharquie KE, Hassen AS, Hassan SA, Al-Hamami IA, 2002. Evaluation of diagnosis of cutaneous leishmaniasis by direct smear, culture and histopathology. Saudi Med J 23: 925–928.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 958 | 647 | 45 |
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Press Imprint Smear: A Rapid, Simple, and Cheap Method for the Diagnosis of Cutaneous Leishmaniasis Caused by Leishmania (Viannia) braziliensis
Anastácio Q. Sousa
Anastácio Q. Sousa
School of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil; Hospital São José for Infectious Diseases, Fortaleza, Ceará, Brazil; Unichristus School of Medicine, Fortaleza, Ceará, Brazil; State University of Ceará School of Medicine, Fortaleza, Ceará, Brazil; University of Virginia School of Medicine, Charlottesville, Virginia
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Margarida M. L. Pompeu
Margarida M. L. Pompeu
School of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil; Hospital São José for Infectious Diseases, Fortaleza, Ceará, Brazil; Unichristus School of Medicine, Fortaleza, Ceará, Brazil; State University of Ceará School of Medicine, Fortaleza, Ceará, Brazil; University of Virginia School of Medicine, Charlottesville, Virginia
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Mércia S. Frutuoso
Mércia S. Frutuoso
School of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil; Hospital São José for Infectious Diseases, Fortaleza, Ceará, Brazil; Unichristus School of Medicine, Fortaleza, Ceará, Brazil; State University of Ceará School of Medicine, Fortaleza, Ceará, Brazil; University of Virginia School of Medicine, Charlottesville, Virginia
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José W. O. Lima
José W. O. Lima
School of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil; Hospital São José for Infectious Diseases, Fortaleza, Ceará, Brazil; Unichristus School of Medicine, Fortaleza, Ceará, Brazil; State University of Ceará School of Medicine, Fortaleza, Ceará, Brazil; University of Virginia School of Medicine, Charlottesville, Virginia
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Juliana M. B. M. Tinel
Juliana M. B. M. Tinel
School of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil; Hospital São José for Infectious Diseases, Fortaleza, Ceará, Brazil; Unichristus School of Medicine, Fortaleza, Ceará, Brazil; State University of Ceará School of Medicine, Fortaleza, Ceará, Brazil; University of Virginia School of Medicine, Charlottesville, Virginia
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Richard D. Pearson
Richard D. Pearson
School of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil; Hospital São José for Infectious Diseases, Fortaleza, Ceará, Brazil; Unichristus School of Medicine, Fortaleza, Ceará, Brazil; State University of Ceará School of Medicine, Fortaleza, Ceará, Brazil; University of Virginia School of Medicine, Charlottesville, Virginia
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A modified imprint method, Press-Imprint-Smear, was compared with histopathology for the diagnosis of cutaneous leishmaniasis. Amastigotes were seen in 69 (92%) of 75 individuals in one or both assays. The Press-Imprint-Smear was positive in 85.3%, and histopathology was positive in 44%. Press-Imprint-Smear is a rapid and relatively sensitive method for the diagnosis of cutaneous leishmaniasis.
Author Notes
Financial support: This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq Grant 485964/2012-0 and Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico-FUNCAP Grant 12535705-2.
Authors' addresses: Anastácio Q. Sousa, Margarida M. L. Pompeu, and Juliana M. B. M. Tinel, School of Medicine, Federal University of Ceará, Fortaleza, Ceará, Brazil, E-mails: aqsousa@gmail.com, mpompeu@ufc.br, and julianazuma@gmail.com. Mércia S. Frutuoso, School of Medicine, Federal University of Ceará and Unichristus School of Medicine, Fortaleza, Ceará, Brazil, E-mail: merciasindeaux@gmail.com. José W. O. Lima, State University of Ceará School of Medicine, Fortaleza, Ceará, Brazil, E-mail: jwolima@yahoo.com.br. Richard D. Pearson, University of Virginia School of Medicine, Charlottesville, VA, E-mail: rdp9g@virginia.edu.
ProCite
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Reference Manager
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-
1.
Alvar J, Velez ID, Bern C, Herero M, Desjeux P, Cano J, Jannin J, den Boer M; WHO Leishmaniasis Control Team, 2012. Leishmaniasis worldwide and global estimates of its incidence. PLoS ONE 7: e35671.
-
2.
Desjeux P, 2004. Leishmaniasis: current situation and new perspectives. Comp Immunol Microbiol Infect Dis 27: 305–318.
-
3.
World Health Organization, 2007. Report of the Fifth Consultative Meeting on Leishmania/HIV Coinfection. Available at: http://www.who.int/leishmaniasis/resources/Leishmaniasis_hiv_coinfection5.pdf. Accessed November 1, 2013.
-
4.
Ministério da Saúde do Brasil, 2013. Casos de Leishmaniose Tegumentar Americana. Brasil. Grandes Regiões e Unidades Federadas. 1990–2011. Available at: http://portal.saude.gov.br/portal/arquivos/pdf/2012_11_casos_de_lta_entre_1990_e_2011.pdf. Accessed November 1, 2013.
-
5.
Levitt J, Bernardo S, Talley Whang T, 2013. How to perform a punch biopsy of the skin—videos in clinical medicine. N Engl J Med 369: e13.
-
6.
De Lorenzi D, Mandara MT, Tranquillo M, Baroni M, Gasparinetti N, Gandini G, Masserdotti C, Bonfanti U, Bertolini G, Vian P, Bernardini M, 2006. Squash–prep cytology in the diagnosis of canine and feline nervous system lesions: a study of 42 cases. Vet Clin Pathol 35: 208–214.
-
7.
Mitra S, Kumar M, Sharma V, Mukhopadhyay D, 2010. Squash preparation: a reliable diagnostic tool in the intraoperative diagnosis of central nervous system tumors. J Cytol 27: 81–85.
-
8.
Alves CF, Alves CF, Figueiredo MM, Souza CC, Machado-Coelho GL, Melo MN, Tafuri WL, Raso P, Soares RP, Tafuri WL, 2013. American tegumentary leishmaniasis: effectiveness of an immunohistochemical protocol for the detection of Leishmania in skin. PLoS ONE 8: e63343.
-
9.
Berman JD, 1997. Human leishmaniasis: clinical, diagnostic, and chemotherapeutic developments in the last 10 years. Clin Infect Dis 24: 684–703.
-
10.
Weigle KA, de Dávalos M, Heredia P, Molineros R, Saravia NG, D'Alessandro A, 1987. Diagnosis of cutaneous and mucocutaneous leishmaniasis in Colombia: a comparison of seven methods. Am J Trop Med Hyg 36: 489–496.
-
11.
Sousa AQ, Parise ME, Pompeu MM, Coelho Filho JM, Vasconcelos IA, Lima JW, Oliveira EG, Vasconcelos AW, David JR, Maguire JH, 1995. Bubonic leishmaniasis: a common manifestation of Leishmania (Viannia) braziliensis infection in Ceara, Brazil. Am J Trop Med Hyg 53: 380–385.
-
12.
Sousa AQ, Pompeu MML, Frutuoso MS, Moraes EA, Pearson RD, 2012. Reply to Drs. Torres and Suarez. Clin Infect Dis 55: 309–310.
-
13.
Aviles H, Belli A, Armijos R, Monroy FP, Harris E, 1999. PCR detection and identification of Leishmania parasites in clinical specimens in Ecuador: a comparison with classical diagnostic methods. J Parasitol 85: 181–187.
-
14.
Ramirez JR, Agudelo S, Muskus C, 2000. Diagnosis of cutaneous leishmaniasis in Colombia: the sampling site within lesions influences the sensitivity of parasitological diagnosis. J Clin Microbiol 38: 3768–3773.
-
15.
Sharquie KE, Hassen AS, Hassan SA, Al-Hamami IA, 2002. Evaluation of diagnosis of cutaneous leishmaniasis by direct smear, culture and histopathology. Saudi Med J 23: 925–928.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 958 | 647 | 45 |
| Full Text Views | 506 | 17 | 0 |
| PDF Downloads | 302 | 13 | 0 |