• 1.

    Currie BJ, Sutherland SK, Hudson BJ, Smith AM, 1991. An epidemiological study of snakebite envenomation in Papua New Guinea. Med J Aust 154: 266268.

    • Search Google Scholar
    • Export Citation
  • 2.

    Lalloo DG, Trevett AJ, Korinhona A, Nwokolo N, Laurenson IF, Paul M, Black J, Naraqi S, Mavo B, Saweri A, Hutton RA, Theakston RDG, Warrell DA, 1995. Snake bites by the Papuan taipan (Oxyuranus scutellatus canni): paralysis, hemostatic and electrocardiographic abnormalities, and effects of antivenom. Am J Trop Med Hyg 52: 525531.

    • Search Google Scholar
    • Export Citation
  • 3.

    Williams D, 2005. Snakebite in Papua New Guinea. Williams DJ, Jensen SD, Nimorakiotakis B, Winkel KD, eds. Venomous Bites and Stings in Papua New Guinea: A Treatment Guide for Health Workers and Doctors. Melbourne, Australia: University of Melbourne, 532.

    • Search Google Scholar
    • Export Citation
  • 4.

    Lalloo DG, Trevett AJ, Owens D, Minei J, Naraqi S, Saweri A, Hutton RA, Theakston RDG, Warrell DA, 1995. Coagulopathy following bites by the Papuan taipan (Oxyuranus scutellatus canni). Blood Coagul Fibrinolysis 6: 6572.

    • Search Google Scholar
    • Export Citation
  • 5.

    Lalloo DG, Trevett AJ, Nwokolo N, Laurenson IF, Naraqi S, Kevau I, Kemp MW, James R, Hooper L, Theakston RDG, Warrell DA, 1997. Electrocardiographic abnormalities in patients bitten by taipans (Oxyuranus scutellatus canni) and other elapid snakes in Papua New Guinea. Trans R Soc Trop Med Hyg 91: 5356.

    • Search Google Scholar
    • Export Citation
  • 6.

    Trevett AJ, Lalloo DG, Nwokolo NC, Naraqi S, Kevau IH, Theakston RDG, Warrell DA, 1995. Electrophysiological findings in patients envenomed following the bite of a Papuan taipan (Oxyuranus scutellatus canni). Trans R Soc Trop Med Hyg 89: 415417.

    • Search Google Scholar
    • Export Citation
  • 7.

    O'Leary MA, Isbister GK, 2009. Commercial monovalent antivenoms in Australia are polyvalent. Toxicon 54: 192195.

  • 8.

    Vargas M, Segura A, Herrera M, Villalta M, Estrada R, Cerdas M, Paiva O, Matainaho T, Jensen SD, Winkel KD, León G, Gutiérrez JM, Williams DJ, 2011. Preclinical evaluation of caprylic acid-fractionated IgG antivenom for the treatment of taipan (Oxyuranus scutellatus) envenoming in Papua New Guinea. PLoS Negl Trop Dis 5: e1144.

    • Search Google Scholar
    • Export Citation
  • 9.

    Lomonte B, Escolano J, Fernández J, Sanz L, Angulo Y, Gutiérrez JM, Calvete JJ, 2008. Snake venomics and antivenomics of the arboreal neotropical pitvipers Bothriechis lateralis and Bothriechis schlegelii. J Proteome Res 7: 24452457.

    • Search Google Scholar
    • Export Citation
  • 10.

    Gutiérrez JM, Lomonte B, León G, Alape-Girón A, Flores-Díaz M, Sanz L, Angulo Y, Calvete JJ, 2009. Snake venomics and antivenomics: proteomic tools in the design and control of antivenoms for the treatment of snakebite envenoming. J Proteomics 72: 165182.

    • Search Google Scholar
    • Export Citation
  • 11.

    Calvete JJ, 2011. Proteomic tools against the neglected pathology of snake bite envenoming. Expert Rev Proteomics 8: 739758.

  • 12.

    Pla D, Gutiérrez JM, Calvete JJ, 2012. Second generation snake antivenomics: comparing immunoaffinity and immunodepletion protocols. Toxicon 60: 688699.

    • Search Google Scholar
    • Export Citation
  • 13.

    Williams DJ, Gutiérrez JM, Calvete JJ, Wüster W, Ratanabanangkoon K, Paiva O, Brown NI, Casewell NR, Harrison RA, Rowley PD, O'Shea M, Jensen SD, Winkel KD, Warrell DA, 2011. Ending the drought: new strategies for improving the flow of affordable, effective antivenoms in Asia and Africa. J Proteomics 74: 17351767.

    • Search Google Scholar
    • Export Citation
  • 14.

    Herrera M, Fernández J, Vargas M, Villalta M, Segura Á, León G, Angulo Y, Paiva O, Matainaho T, Jensen SD, Winkel KD, Calvete JJ, Williams DJ, Gutiérrez JM, 2012. Comparative proteomic analysis of the venom of the taipan snake, Oxyuranus scutellatus, from Papua New Guinea and Australia: role of neurotoxic and procoagulant effects in venom toxicity. J Proteomics 75: 21282140.

    • Search Google Scholar
    • Export Citation
  • 15.

    Fohlman J, Eaker D, Karlsson E, Thesleff S, 1976. Taipoxin, an extremely potent presynaptic neurotoxin from the venom of the Australian snake taipan (Oxyuranus s. scutellatus). Isolation, characterization, quaternary structure and pharmacological properties. Eur J Biochem 68: 457469.

    • Search Google Scholar
    • Export Citation
  • 16.

    Lambeau G, Barhanin J, Schweitz H, Qar J, Lazdunski M, 1989. Identification and properties of very high affinity brain membrane-binding sites for a neurotoxic phospholipase from the taipan venom. J Biol Chem 264: 1150311510.

    • Search Google Scholar
    • Export Citation
  • 17.

    Lambeau G, Cupillard L, Lazdunski M, 1997. Membrane receptors for venom phospholipases A2. Kini RM, ed. Venom Phospholipase A2 Enzymes. Structure, Function and Mechanism. Chichester, United Kingdom: Wiley, 389412.

    • Search Google Scholar
    • Export Citation
  • 18.

    Speiger H, Govers-Riemslag JWP, Swaal RFA, Rosing J, 1986. Prothrombin activation by an activator from the venom of Oxyuranus scutellatus (taipan snake). J Biol Chem 261: 1325813267.

    • Search Google Scholar
    • Export Citation
  • 19.

    Calvete JJ, Cid P, Sanz L, Segura A, Villalta M, Herrera M, León G, Harrison R, Durfa N, Nasidi A, Theakston RDG, Warrell DA, Gutiérrez JM, 2010. Antivenomic assessment of the immunological reactivity of EchiTAb-Plus-ICP, an antivenom for the treatment of snakebite envenoming in sub-Saharan Africa. Am J Trop Med Hyg 82: 11941201.

    • Search Google Scholar
    • Export Citation
  • 20.

    Kuruppu S, Reeve S, Banerjee Y, Kini RM, Smith I, Hodgson WC, 2005. Isolation and pharmacological characterization of cannitoxin, a presynaptic neurotoxin from the venom of the Papuan taipan (Oxyuranus scutellatus canni). J Pharmacol Exp Ther 315: 11961202.

    • Search Google Scholar
    • Export Citation
  • 21.

    Possani LD, Martin BM, Yatani A, Mochca-Morales J, Zamudio FZ, Gurrola GB, Brown AM, 1992. Isolation and physiological characterization of taicatoxin, a complex toxin with specific effects on calcium channels. Toxicon 30: 13431364.

    • Search Google Scholar
    • Export Citation
 
 
 
 

 

 
 

 

 

 

 

 

 

Antivenomic Characterization of Two Antivenoms Against the Venom of the Taipan, Oxyuranus scutellatus, from Papua New Guinea and Australia

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  • Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica; Charles Campbell Toxinology Centre, School of Medicine and Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea; Special Laboratory of Applied Toxinology, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, Sao Paulo, Brazil; Australian Venom Research Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, Victoria, Australia

Antivenoms manufactured by bioCSL Limited (Australia) and Instituto Clodomiro Picado (Costa Rica) against the venom of the taipan snakes (Oxyuranus scutellatus) from Australia and Papua New Guinea (PNG), respectively, were compared using antivenomics, an analytical approach that combines proteomics with immunoaffinity chromatography. Both antivenoms recognized all venom proteins present in venom from PNG O. scutellatus, although a pattern of partial recognition was observed for some components. In the case of the Australian O. scutellatus venom, both antivenoms immunorecognized the majority of the components, but the CSL antivenom showed a stronger pattern of immunoreactivity, which was revealed by the percentage of retained proteins in the immunoaffinity column. Antivenoms interacted with taipoxin in surface plasmon resonance. These observations on antivenomics agree with previous neutralization studies.

Author Notes

* Address correspondence to José María Gutiérrez, Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José 2060-1000, Costa Rica. E-mail: jose.gutierrez@ucr.ac.cr

Financial support: This study was supported by Fondos del Sistema-Consejo Nacional de Rectores (FEES-CONARE), Vicerrectoría de Investigación, University of Costa Rica Projects 741-B2-652 and 741-B3-017, and a project grant from the Australian National Health and Medical Research Council (Australia). Additional financial support was also provided through an ad hoc grant from the Papua New Guinea Office of Higher Education.

Authors' addresses: María Herrera, Álvaro Segura, Mariángela Vargas, Mauren Villalta, Guillermo León, and José María Gutiérrez, Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica, E-mails: maria.herrera_v@ucr.ac.cr, alvaro.seguraruiz@ucr.ac.cr, mariangela.vargasarroyo@ucr.ac.cr, mauren.villaltaarrieta@ucr.ac.cr, guillermo.leon@ucr.ac.cr, and jose.gutierrez@ucr.ac.cr. Owen K. Paiva, Charles Campbell Toxinology Centre, School of Medicine and Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea, E-mail: owen.paiva@gmail.com. Ana Helena Pagotto and Solange M. T. Serrano, Special Laboratory of Applied Toxinology, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, Sao Paulo, Brazil, E-mails: ana.pagotto@butantan.gov.br and solange.serrano@butantan.gov.br. Simon D. Jensen and David J. Williams, Charles Campbell Toxinology Centre, School of Medicine and Health Sciences, University of Papua New Guinea, Port Moresby, Papua New Guinea, and Australian Venom Research Unit, Department of Pharmacology and Therapeutics, University of Melbourne, Parkville, VIC, Australia, E-mails: simondjensen@hotmail.com and david.williams@unimelb.edu.au.

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