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Sperm Morphological Features Associated with Chronic Chagas Disease in the Semen of Experimentally Infected Dogs

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  • Department of Molecular Biology, Instituto Nacional de Cardiología “Ignacio Chávez,” Mexico City, Mexico; Department of Agricultural and Animal Production, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma Metropolitana Unidad Xochimilco, Mexico City, Mexico; Department of Parasitology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico; Department of Pathological Anatomy, Instituto Nacional de Cardiología “Ignacio Chávez,” Mexico City, Mexico

The presence of trypanosomatids in the reproductive systems of different mammals (causing genital lesions in the acute stage of the disease) may predispose the animals to low semen quality. However, there are no studies examining the alterations in the sperm morphological features in the chronic stage of Trypanosoma cruzi infection. Knowledge of these aspects is important to understand the other ways of transmission of the Chagas disease. Progressive motility, mass motility, concentration, and sperm morphology of 84 ejaculates of dogs that were chronically infected with T. cruzi were evaluated. Most of the findings were consistent with the reference values and with those obtained from healthy control dogs. The scrotal circumference was not correlated with spermatozoa concentration in the infected animals. In conclusion, the T. cruzi Ninoa (MHOM/MX/1994/Ninoa) strain does not cause significant alterations in the semen quality of dogs experiencing chronic Chagas disease (at concentrations of 5 × 104 to 1 × 106 parasites per animal).

Author Notes

* Address correspondence to Minerva Arce-Fonseca, Department of Molecular Biology, Instituto Nacional de Cardiología “Ignacio Chávez,” Juan Badiano No. 1, Col. Sección XVI, Tlalpan, Mexico City, 14080, Mexico. E-mail: mini_arce@yahoo.com.mx

Financial support: This work was supported by a grant from the Instituto de Ciencia y Tecnología del Distrito Federal (ICyTDF), Mexico (Grant 236/2010).

Authors' addresses: Olivia Rodríguez-Morales, Elvia Pedro-Martínez, and Minerva Arce-Fonseca, Department of Molecular Biology, Instituto Nacional de Cardiología “Ignacio Chávez,” Mexico City, Mexico, E-mails: rm.olivia@gmail.com, mvz.elvia@hotmail.com, and mini_arce@yahoo.com.mx. José Ernesto Hernández-Pichardo, Department of Agricultural and Animal Production, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma Metropolitana Unidad Xochimilco, Mexico City, Mexico, E-mail: ehernan@correo.xoc.uam.mx. Ricardo Alejandre-Aguilar, Department of Parasitology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico, E-mail: rialejandre@yahoo.com.mx. Alberto Aranda-Fraustro and Verónica Graullera-Rivera, Department of Pathological Anatomy, Instituto Nacional de Cardiología “Ignacio Chávez,” Mexico City, Mexico, E-mails: arandafraustro@yahoo.com.mx and grauvero@yahoo.com.mx.

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