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Vector Competence of Aedes aegypti and Aedes vittatus (Diptera: Culicidae) from Senegal and Cape Verde Archipelago for West African Lineages of Chikungunya Virus

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  • Institut Pasteur de Dakar, Dakar, Senegal; Institute for Human Infections and Immunity, Center for Biodefense and Emerging Infectious Diseases, and Department of Pathology, University of Texas Medical Branch, Galveston, Texas; Université Cheikh Anta Diop de Dakar, Dakar, Senegal

To assess the risk of emergence of chikungunya virus (CHIKV) in West Africa, vector competence of wild-type, urban, and non-urban Aedes aegypti and Ae. vittatus from Senegal and Cape Verde for CHIKV was investigated. Mosquitoes were fed orally with CHIKV isolates from mosquitoes (ArD30237), bats (CS13-288), and humans (HD180738). After 5, 10, and 15 days of incubation following an infectious blood meal, presence of CHIKV RNA was determined in bodies, legs/wings, and saliva using real-time reverse transcription–polymerase chain reaction. Aedes vittatus showed high susceptibility (50–100%) and early dissemination and transmission of all CHIKV strains tested. Aedes aegypti exhibited infection rates ranging from 0% to 50%. Aedes aegypti from Cape Verde and Kedougou, but not those from Dakar, showed the potential to transmit CHIKV in saliva. Analysis of biology and competence showed relatively high infective survival rates for Ae. vittatus and Ae. aegypti from Cape Verde, suggesting their efficient vector capacity in West Africa.

Author Notes

* Address correspondence to Mawlouth Diallo, Unité d'Entomologie Médicale, Institut Pasteur de Dakar, BP 220 Dakar, Senegal. E-mail: diallo@pasteur.sn

Financial support: This study was supported by the National Institutes of Health (grant RO1AI069145).

Authors' addresses: Cheikh T. Diagne, Diawo Diallo, Yamar Ba, Ibrahima Dia, and Mawlouth Diallo, Unité d'Entomologie Médicale, Institut Pasteur de Dakar, Dakar, Senegal, E-mails: ctdiagne@pasteur.sn, diawod@yahoo.com, ba@pasteur.sn, dia@pasteur.sn, and diallo@pasteur.sn. Oumar Faye and Amadou A. Sall, Unite des Arbovirus et Virus de Fièvres Hémorragiques, Institut Pasteur de Dakar, Dakar, Senegal, E-mails: oumarfaye@pasteur.sn and asall@pasteur.sn. Mathilde Guerbois, Rachel Knight, and Scott C. Weaver, Institute for Human Infections and Immunity, Center for Tropical Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, TX, E-mails: maguerbo@utmb.edu, raknight@utmb.edu, and sweaver@utmb.edu. Ousmane Faye, Unite des Arbovirus et Virus de Fièvres Hémorragiques, Institut Pasteur de Dakar, Dakar, Senegal, and Laboratoire d'Ecologie Vectorielle et Parasitaire, Département de Biologie Animale, Université Cheikh Anta Diop, Dakar, Senegal, E-mails: ofaye@pasteur.sn and fayeo@orange.sn.

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