Author:
ProCite
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Reference Manager
BibTeX
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-
1.
Lee AC, Montgomery SP, Theis JH, Blagburn BL, Eberhard ML, 2010. Public health issues concerning the widespread distribution of canine heartworm disease. Trends Parasitol 26: 168–173.
-
2.
Ettinger S, Feldman E, 2005. Textbook of Small Animal Veterinary Medicine. St. Louis, MO: Elsevier Saunders.
-
3.
Atkins C, 2005. Canine heartworm disease. Textbook of Veterinary Internal Medicine. Sixth edition. St. Louis, MO: Elsevier, 1118–1136.
-
4.
Simón F, Morchón R, González-Miguel J, Marcos-Atxutegi C, Siles-Lucas M, 2009. What is new about animal and human dirofilariosis? Trends Parasitol 25: 404–409.
-
5.
Morchón R, Moya I, González-Miguel J, Montoya M, Simon F, 2010. Zoonotic Dirofilaria immitis infections in a province of northern Spain. Epidemiol Infect 138: 380–383.
-
6.
Avellis FO, Kramer LH, Mora P, Bartolino A, Benedetti P, Rivasi F, 2011. A Case of human conjunctival dirofilariosis by Dirofilaria immitis in Italy. Vector Borne Zoonotic Dis 11: 451–452.
-
7.
Otranto D, Diniz DG, Dantas-Torres F, Casiraghi M, de Almeida IN, de Almeida LN, dos Santos JN, Furtado AP, de Almeida Sobrinho EF, Bain O, 2011. Human intraocular filariasis caused by Dirofilaria sp. nematode, Brazil. Emerg Infect Dis 17: 863.
-
8.
To KK, Wong SS, Poon RW, Trendell-Smith NJ, Ngan AH, Lam JW, Tang TH, AhChong A-K, Kan JC, Chan K-H, Yuen KY, 2012. A novel Dirofilaria species causing human and canine infections in Hong Kong. J Clin Microbiol 50: 3534–3541.
-
9.
Foissac M, Million M, Mary C, Dales J-P, Souraud J-B, Piarroux R, Parola P, 2013. Subcutaneous infection with Dirofilaria immitis nematode in human, France. Emerg Infect Dis 19: 171.
-
10.
Montoya-Alonso JA, Mellado I, Carretón E, Cabrera-Pedrero ED, Morchón R, Simón F, 2010. Canine dirofilariosis caused by Dirofilaria immitis is a risk factor for the human population on the island of Gran Canaria, Canary Islands, Spain. Parasitol Res 107: 1265–1269.
-
11.
Fu Y, Lan J, Zhang Z, Hou R, Wu X, Yang D, Zhang R, Zheng W, Nie H, Xie Y, 2012. Novel insights into the transcriptome of Dirofilaria immitis. PLoS ONE 7: e41639.
-
12.
Geary J, Satti M, Moreno Y, Madrill N, Whitten D, Headley SA, Agnew D, Geary T, Mackenzie C, 2012. First analysis of the secretome of the canine heartworm, Dirofilaria immitis. Parasit Vectors 5: 140.
-
13.
Godel C, Kumar S, Koutsovoulos G, Ludin P, Nilsson D, Comandatore F, Wrobel N, Thompson M, Schmid CD, Goto S, 2012. The genome of the heartworm, Dirofilaria immitis, reveals drug and vaccine targets. FASEB J 26: 4650–4661.
-
14.
Fu Y, Lan J, Wu X, Yang D, Zhang Z, Nie H, Hou R, Zhang R, Zheng W, Xie Y, 2013. Identification of Dirofilaria immitis miRNA using illumina deep sequencing. Vet Res 44: 3.
-
15.
Huang H, Wang T, Yang G, Zhang Z, Wang C, Yang Z, Luo L, Liu L, Lan J, Huang X, 2009. Molecular characterization and phylogenetic analysis of Dirofilaria immitis of China based on COI and 12S rDNA genes. Vet Parasitol 160: 175–179.
-
16.
Casiraghi M, Bazzocchi C, Mortarino M, Ottina E, Genchi C, 2006. A simple molecular method for discriminating common filarial nematodes of dogs (Canis familiaris). Vet Parasitol 141: 368–372.
-
17.
Sambrook J, Russell DW, 2001. Molecular Cloning: A Laboratory Manual. Long Island, NY: CSHL Press.
-
18.
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG, 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–4882.
-
19.
Kimura M, 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111–120.
-
20.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S, 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739.
-
21.
Ronquist F, Huelsenbeck JP, 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574.
-
22.
Yatawara L, Wickramasinghe S, Nagataki M, Rajapakse R, Agatsuma T, 2007. Molecular characterization and phylogenetic analysis of Setaria digitata of Sri Lanka based on CO1 and 12S rDNA genes. Vet Parasitol 148: 161–165.
-
23.
Casiraghi M, Bain O, Guerrero R, Martin C, Pocacqua V, Gardner SL, Franceschi A, Bandi C, 2004. Mapping the presence of Wolbachia pipientis on the phylogeny of filarial nematodes: evidence for symbiont loss during evolution. Int J Parasitol 34: 191–203.
-
24.
Casiraghi M, Anderson T, Bandi C, Bazzocchi C, Genchi C, 2001. A phylogenetic analysis of filarial nematodes: comparison with the phylogeny of Wolbachia endosymbionts. Parasitology 122: 93–103.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 29 | 29 | 8 |
| Full Text Views | 373 | 95 | 0 |
| PDF Downloads | 70 | 21 | 0 |
Intraspecific Genetic Variation and Phylogenetic Analysis of Dirofilaria immitis Samples from Western China Using Complete ND1 and 16S rDNA Gene Sequences
Tianyu Liu
Tianyu Liu
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China
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Yinan Liang
Yinan Liang
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China
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Xiuqin Zhong
Xiuqin Zhong
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China
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Ning Wang
Ning Wang
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China
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Dandan Hu
Dandan Hu
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China
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Xuan Zhou
Xuan Zhou
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China
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Xiaobin Gu
Xiaobin Gu
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China
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Xuerong Peng
Xuerong Peng
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China
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Guangyou Yang
Guangyou Yang
Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China
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Dirofilaria immitis (heartworm) is the causative agent of an important zoonotic disease that is spread by mosquitoes. In this study, molecular and phylogenetic characterization of D. immitis were performed based on complete ND1 and 16S rDNA gene sequences, which provided the foundation for more advanced molecular diagnosis, prevention, and control of heartworm diseases. The mutation rate and evolutionary divergence in adult heartworm samples from seven dogs in western China were analyzed to obtain information on genetic diversity and variability. Phylogenetic relationships were inferred using both maximum parsimony (MP) and Bayes methods based on the complete gene sequences. The results suggest that D. immitis formed an independent monophyletic group in which the 16S rDNA gene has mutated more rapidly than has ND1.
Author Notes
Authors' addresses: Tianyu Liu, Yinan Liang, Xiuqin Zhong, Ning Wang, Dandan Hu, Xuan Zhou, and Xiaobin Gu, Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China, E-mails: kevinlau1990@hotmail.com, susieconan@vip.qq.com, xiuqinzhong@hotmail.com, wangningzhuhui@hotmail.com, 18783542005@163.com, zhouxuan198866@163.com, and guxiaobin198225@126.com. Xuerong Peng, Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China, E-mail: 200762130@qq.com. Guangyou Yang, Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China, E-mail: guangyouyang@hotmail.com.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Lee AC, Montgomery SP, Theis JH, Blagburn BL, Eberhard ML, 2010. Public health issues concerning the widespread distribution of canine heartworm disease. Trends Parasitol 26: 168–173.
-
2.
Ettinger S, Feldman E, 2005. Textbook of Small Animal Veterinary Medicine. St. Louis, MO: Elsevier Saunders.
-
3.
Atkins C, 2005. Canine heartworm disease. Textbook of Veterinary Internal Medicine. Sixth edition. St. Louis, MO: Elsevier, 1118–1136.
-
4.
Simón F, Morchón R, González-Miguel J, Marcos-Atxutegi C, Siles-Lucas M, 2009. What is new about animal and human dirofilariosis? Trends Parasitol 25: 404–409.
-
5.
Morchón R, Moya I, González-Miguel J, Montoya M, Simon F, 2010. Zoonotic Dirofilaria immitis infections in a province of northern Spain. Epidemiol Infect 138: 380–383.
-
6.
Avellis FO, Kramer LH, Mora P, Bartolino A, Benedetti P, Rivasi F, 2011. A Case of human conjunctival dirofilariosis by Dirofilaria immitis in Italy. Vector Borne Zoonotic Dis 11: 451–452.
-
7.
Otranto D, Diniz DG, Dantas-Torres F, Casiraghi M, de Almeida IN, de Almeida LN, dos Santos JN, Furtado AP, de Almeida Sobrinho EF, Bain O, 2011. Human intraocular filariasis caused by Dirofilaria sp. nematode, Brazil. Emerg Infect Dis 17: 863.
-
8.
To KK, Wong SS, Poon RW, Trendell-Smith NJ, Ngan AH, Lam JW, Tang TH, AhChong A-K, Kan JC, Chan K-H, Yuen KY, 2012. A novel Dirofilaria species causing human and canine infections in Hong Kong. J Clin Microbiol 50: 3534–3541.
-
9.
Foissac M, Million M, Mary C, Dales J-P, Souraud J-B, Piarroux R, Parola P, 2013. Subcutaneous infection with Dirofilaria immitis nematode in human, France. Emerg Infect Dis 19: 171.
-
10.
Montoya-Alonso JA, Mellado I, Carretón E, Cabrera-Pedrero ED, Morchón R, Simón F, 2010. Canine dirofilariosis caused by Dirofilaria immitis is a risk factor for the human population on the island of Gran Canaria, Canary Islands, Spain. Parasitol Res 107: 1265–1269.
-
11.
Fu Y, Lan J, Zhang Z, Hou R, Wu X, Yang D, Zhang R, Zheng W, Nie H, Xie Y, 2012. Novel insights into the transcriptome of Dirofilaria immitis. PLoS ONE 7: e41639.
-
12.
Geary J, Satti M, Moreno Y, Madrill N, Whitten D, Headley SA, Agnew D, Geary T, Mackenzie C, 2012. First analysis of the secretome of the canine heartworm, Dirofilaria immitis. Parasit Vectors 5: 140.
-
13.
Godel C, Kumar S, Koutsovoulos G, Ludin P, Nilsson D, Comandatore F, Wrobel N, Thompson M, Schmid CD, Goto S, 2012. The genome of the heartworm, Dirofilaria immitis, reveals drug and vaccine targets. FASEB J 26: 4650–4661.
-
14.
Fu Y, Lan J, Wu X, Yang D, Zhang Z, Nie H, Hou R, Zhang R, Zheng W, Xie Y, 2013. Identification of Dirofilaria immitis miRNA using illumina deep sequencing. Vet Res 44: 3.
-
15.
Huang H, Wang T, Yang G, Zhang Z, Wang C, Yang Z, Luo L, Liu L, Lan J, Huang X, 2009. Molecular characterization and phylogenetic analysis of Dirofilaria immitis of China based on COI and 12S rDNA genes. Vet Parasitol 160: 175–179.
-
16.
Casiraghi M, Bazzocchi C, Mortarino M, Ottina E, Genchi C, 2006. A simple molecular method for discriminating common filarial nematodes of dogs (Canis familiaris). Vet Parasitol 141: 368–372.
-
17.
Sambrook J, Russell DW, 2001. Molecular Cloning: A Laboratory Manual. Long Island, NY: CSHL Press.
-
18.
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG, 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–4882.
-
19.
Kimura M, 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111–120.
-
20.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S, 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739.
-
21.
Ronquist F, Huelsenbeck JP, 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574.
-
22.
Yatawara L, Wickramasinghe S, Nagataki M, Rajapakse R, Agatsuma T, 2007. Molecular characterization and phylogenetic analysis of Setaria digitata of Sri Lanka based on CO1 and 12S rDNA genes. Vet Parasitol 148: 161–165.
-
23.
Casiraghi M, Bain O, Guerrero R, Martin C, Pocacqua V, Gardner SL, Franceschi A, Bandi C, 2004. Mapping the presence of Wolbachia pipientis on the phylogeny of filarial nematodes: evidence for symbiont loss during evolution. Int J Parasitol 34: 191–203.
-
24.
Casiraghi M, Anderson T, Bandi C, Bazzocchi C, Genchi C, 2001. A phylogenetic analysis of filarial nematodes: comparison with the phylogeny of Wolbachia endosymbionts. Parasitology 122: 93–103.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 29 | 29 | 8 |
| Full Text Views | 373 | 95 | 0 |
| PDF Downloads | 70 | 21 | 0 |