Intraspecific Genetic Variation and Phylogenetic Analysis of Dirofilaria immitis Samples from Western China Using Complete ND1 and 16S rDNA Gene Sequences

Tianyu Liu Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China

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Yinan Liang Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China

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Xiuqin Zhong Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China

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Ning Wang Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China

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Dandan Hu Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China

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Xuan Zhou Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China

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Xiaobin Gu Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China

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Xuerong Peng Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China

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Guangyou Yang Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China; Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China

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Dirofilaria immitis (heartworm) is the causative agent of an important zoonotic disease that is spread by mosquitoes. In this study, molecular and phylogenetic characterization of D. immitis were performed based on complete ND1 and 16S rDNA gene sequences, which provided the foundation for more advanced molecular diagnosis, prevention, and control of heartworm diseases. The mutation rate and evolutionary divergence in adult heartworm samples from seven dogs in western China were analyzed to obtain information on genetic diversity and variability. Phylogenetic relationships were inferred using both maximum parsimony (MP) and Bayes methods based on the complete gene sequences. The results suggest that D. immitis formed an independent monophyletic group in which the 16S rDNA gene has mutated more rapidly than has ND1.

Author Notes

* Address correspondence to Guangyou Yang, Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China. E-mail: guangyouyang@hotmail.com

Authors' addresses: Tianyu Liu, Yinan Liang, Xiuqin Zhong, Ning Wang, Dandan Hu, Xuan Zhou, and Xiaobin Gu, Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China, E-mails: kevinlau1990@hotmail.com, susieconan@vip.qq.com, xiuqinzhong@hotmail.com, wangningzhuhui@hotmail.com, 18783542005@163.com, zhouxuan198866@163.com, and guxiaobin198225@126.com. Xuerong Peng, Department of Chemistry, College of Life and Science, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China, E-mail: 200762130@qq.com. Guangyou Yang, Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an City, Sichuan Province, People's Republic of China, E-mail: guangyouyang@hotmail.com.

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