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-
1.
WHO, 2010. Report of an Informal Working Group on Urogenital Schistosomiasis and HIV transmission, Geneva: World Health Organization, 1–35.
-
2.
Downs JA, van Dam GJ, Andreasen A, van Lieshout L, Corstjens PLAM, Kalluvya SE, Fitzgerald DW, de Dood CJ, Peck RN, Johnson WD, Bang H, Changalucha JM, 2012. Association of schistosomiasis and HIV infection in Tanzania. Am J Trop Med Hyg 87: 868–873.
-
3.
Kjetland EF, Mduluza T, Ndhlovu PD, Gomo E, Gwanzura L, Midzi N, Mason P, Friis H, Gundersen SG, 2006. Genital schistosomiasis in women: a clinical 12-month in vivo study following treatment with praziquantel. Trans R Soc Trop Med Hyg 100: 740–752.
-
4.
Mbabazi PS, Andan O, Fitzgerald DW, Chitsulo L, Engels D, Jennifer A, 2011. Examining the relationship between urogenital schistosomiasis and HIV Infection. PLoS Negl Trop Dis 5: 1–8.
-
5.
Aryeetey YA, Essien–Baidoo S, Larbi IA, Ahmed K, Amoah AS, Obeng BB, van Lieshout L, Yazdanbaksh M, Boakye DA, Verweij JJ, 2013. Molecular diagnosis of Schistosoma infections in urine samples of school children in Ghana. Am J Trop Med Hyg 88: 1028–1031.
-
6.
Cnops L, Soentjens P, Clerinx J, Van Esbroeck M, 2013. A Schistosoma haematobium-specific real-time PCR for diagnosis of urogenital schistosomiasis in serum samples of international travelers and migrants. PLoS Negl Trop Dis 7: e2413.
-
7.
Yin-chang Z, Socheat D, Bounlu K, You-sheng L, Sinuon M, Insisiengmay S, Wei H, Wei–Zhu S, Bergquist R, 2005. Application of dipstick dye immunoassay (DDIA) kit for the diagnosis of schistosomiasis mekongi. Acta Trop 96: 137–141.
-
8.
Angeles JMM, Goto Y, Kirinoki M, Asada M, Leonardo LR, Rivera PT, Villacorte EA, Inoue N, Chigusa Y, Kawazu S, 2012. Utilization of ELISA using thioredoxin peroxidase-1 and tandem repeat proteins for diagnosis of Schistosoma japonicum infection among water buffaloes. PLoS Negl Trop Dis 6: e1800.
-
9.
Doenhoff MJ, Wheeler JG, Tricker K, Hamilton JV, Sturrock RF, Butterworth AE, Ouma JH, Mbugua GG, Kariuki C, Koech D, 2003. The detection of antibodies against Schistosoma mansoni soluble egg antigens (SEA) and CEF6 in ELISA, before and after chemotherapy. Ann Trop Med Parasitol 97: 697–709.
-
10.
Zhu Y, Hua W, Xu M, He W, Wang X, Dai Y, Zhao S, Tang J, Wang S, Lu S, 2012. A novel immunodiagnostic assay to detect serum antibody response against selected soluble egg antigen fractions from Schistosoma japonicum. PLoS ONE 7: e44032.
-
11.
Xu J, Rong R, Zhang HQ, Shi CJ, Zhu XQ, Xia CM, 2009. Sensitive and rapid detection of Schistosoma japonicum DNA by loop-mediated isothermal amplification (LAMP). Int J Parasitol 40: 327–331.
-
12.
Wichmann D, Panning M, Quack T, Kramme S, Burchard G, Grevelding C, Drosten C, 2009. Diagnosing schistosomiasis by detection of cell-free parasite DNA in human plasma. PLoS Negl Trop Dis 3: e422.
-
13.
Wichmann D, Poppert S, Von Thien H, Clerinx J, Dieckmann S, Jensenius M, Parola P, Richter J, Schunk M, Stich A, Zanger P, Burchard G, Tannich E, 2013. Prospective European-wide multicentre study on a blood based real-time PCR for the diagnosis of acute schistosomiasis. BMC Infect Dis 13: 1–7.
-
14.
Ibironke O, Koukounari A, Asaolu S, Moustaki I, Shiff C, 2012. Validation of a new test for Schistosoma haematobium based on detection of Dra1 DNA fragments in urine: evaluation through latent class analysis. PLoS Negl Trop Dis 6: e1464.
-
15.
Van Riet E, Adegnika AA, Retra K, Vieira R, Tielens AGM, Lell B, Yazdanbakhsh M, 2007. Cellular and humoral responses to influenza in gabonese children living in rural and semi-urban areas. J Infect Dis 196: 1671–1678.
-
16.
Obeng BB, Aryeetey YA, de Dood CJ, Amoah AS, Larbi IA, Deelder AM, van Lieshout L, 2008. Application of a circulating-cathodic-antigen (CCA) strip test and real-time PCR, in comparison with microscopy, for the detection of Schistosoma haematobium in urine samples from Ghana. Ann Trop Med Parasitol 102: 625–633.
-
17.
Niesters HG, 2002. Clinical virology in real time. J Clin Virol 25: 3–12.
-
18.
Fernández-Soto P, Velasco Tirado V, Carranza Rodríguez C, Pérez-Arellano JL, Muro A, 2013. Long-term frozen storage of urine samples: a trouble to get PCR results in Schistosoma spp. DNA detection? PLoS ONE 8: e61703.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 41 | 41 | 5 |
| Full Text Views | 259 | 73 | 1 |
| PDF Downloads | 86 | 21 | 1 |
Impact of Short-Time Urine Freezing on the Sensitivity of an Established Schistosoma Real-Time PCR Assay
Hilaire M. Kenguele
Hilaire M. Kenguele
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands
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Ayola A. Adegnika
Ayola A. Adegnika
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands
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Anne-Marie Nkoma
Anne-Marie Nkoma
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands
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Ulysse Ateba-Ngoa
Ulysse Ateba-Ngoa
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands
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Mirabeau Mbong
Mirabeau Mbong
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands
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Jeannot Zinsou
Jeannot Zinsou
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands
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Bertrand Lell
Bertrand Lell
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands
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Jaco J. Verweij
Jaco J. Verweij
Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon; Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany; Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands; Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands
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Urogenital schistosomiaisis is a serious public health problem in sub-Saharan Africa. In this study, we have updated an established real-time polymerase chain reaction (PCR) routinely used in our laboratory. Schistosoma genus-specific real-time PCR was performed on DNA isolated from 85 urine samples and pellets obtained after centrifugation without and after frozen storage. The results revealed that concentration by centrifugation of the urine samples and freezing of the samples before extracting DNA improves the sensitivity of the PCR.
Author Notes
Financial support: EDCTP Senior Fellowship TA 11 40200025 Deutsche Forschungsgemeinschaft-funded project Deutsch-Afrikanische Kooperationsprojekte in der Infektiologie (DFG-Projekt KR 1150/6-1), EU-funded project Immunological Interplay between Poverty Related Diseases and Helminth infections: An African-European Research Initiative “IDEA” (HEALTH-F3-2009-241642). EU-Funded projects: The targeted development of a new generation Vaccine for Schistosomiasis “TheSchistoVac” (Health-2009-242107).
Authors' addresses: Hilaire M. Kenguele, Département de Biologie, Université des Sciences et Techniques de Masuku (USTM), Franceville, Gabon, E-mail: hkenguele@gmail.com. Ayola A. Adegnika, Anne-Marie Nkoma, Ulysse Ateba-Ngoa, Mirabeau Mbong, Jeannot Zinsou, and Bertrand Lell, Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon, Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany, Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands, E-mails: aadegnika@gmail.com, mymhankoma@yahoo.fr, ulyssus7000@gmail.com, mirabs2006@yahoo.com, zinaff@gmail.com, and Bertrand.lell@gmail.com. Jaco J. Verweij, Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands, E-mail: j.verweij@elisabeth.nl.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
WHO, 2010. Report of an Informal Working Group on Urogenital Schistosomiasis and HIV transmission, Geneva: World Health Organization, 1–35.
-
2.
Downs JA, van Dam GJ, Andreasen A, van Lieshout L, Corstjens PLAM, Kalluvya SE, Fitzgerald DW, de Dood CJ, Peck RN, Johnson WD, Bang H, Changalucha JM, 2012. Association of schistosomiasis and HIV infection in Tanzania. Am J Trop Med Hyg 87: 868–873.
-
3.
Kjetland EF, Mduluza T, Ndhlovu PD, Gomo E, Gwanzura L, Midzi N, Mason P, Friis H, Gundersen SG, 2006. Genital schistosomiasis in women: a clinical 12-month in vivo study following treatment with praziquantel. Trans R Soc Trop Med Hyg 100: 740–752.
-
4.
Mbabazi PS, Andan O, Fitzgerald DW, Chitsulo L, Engels D, Jennifer A, 2011. Examining the relationship between urogenital schistosomiasis and HIV Infection. PLoS Negl Trop Dis 5: 1–8.
-
5.
Aryeetey YA, Essien–Baidoo S, Larbi IA, Ahmed K, Amoah AS, Obeng BB, van Lieshout L, Yazdanbaksh M, Boakye DA, Verweij JJ, 2013. Molecular diagnosis of Schistosoma infections in urine samples of school children in Ghana. Am J Trop Med Hyg 88: 1028–1031.
-
6.
Cnops L, Soentjens P, Clerinx J, Van Esbroeck M, 2013. A Schistosoma haematobium-specific real-time PCR for diagnosis of urogenital schistosomiasis in serum samples of international travelers and migrants. PLoS Negl Trop Dis 7: e2413.
-
7.
Yin-chang Z, Socheat D, Bounlu K, You-sheng L, Sinuon M, Insisiengmay S, Wei H, Wei–Zhu S, Bergquist R, 2005. Application of dipstick dye immunoassay (DDIA) kit for the diagnosis of schistosomiasis mekongi. Acta Trop 96: 137–141.
-
8.
Angeles JMM, Goto Y, Kirinoki M, Asada M, Leonardo LR, Rivera PT, Villacorte EA, Inoue N, Chigusa Y, Kawazu S, 2012. Utilization of ELISA using thioredoxin peroxidase-1 and tandem repeat proteins for diagnosis of Schistosoma japonicum infection among water buffaloes. PLoS Negl Trop Dis 6: e1800.
-
9.
Doenhoff MJ, Wheeler JG, Tricker K, Hamilton JV, Sturrock RF, Butterworth AE, Ouma JH, Mbugua GG, Kariuki C, Koech D, 2003. The detection of antibodies against Schistosoma mansoni soluble egg antigens (SEA) and CEF6 in ELISA, before and after chemotherapy. Ann Trop Med Parasitol 97: 697–709.
-
10.
Zhu Y, Hua W, Xu M, He W, Wang X, Dai Y, Zhao S, Tang J, Wang S, Lu S, 2012. A novel immunodiagnostic assay to detect serum antibody response against selected soluble egg antigen fractions from Schistosoma japonicum. PLoS ONE 7: e44032.
-
11.
Xu J, Rong R, Zhang HQ, Shi CJ, Zhu XQ, Xia CM, 2009. Sensitive and rapid detection of Schistosoma japonicum DNA by loop-mediated isothermal amplification (LAMP). Int J Parasitol 40: 327–331.
-
12.
Wichmann D, Panning M, Quack T, Kramme S, Burchard G, Grevelding C, Drosten C, 2009. Diagnosing schistosomiasis by detection of cell-free parasite DNA in human plasma. PLoS Negl Trop Dis 3: e422.
-
13.
Wichmann D, Poppert S, Von Thien H, Clerinx J, Dieckmann S, Jensenius M, Parola P, Richter J, Schunk M, Stich A, Zanger P, Burchard G, Tannich E, 2013. Prospective European-wide multicentre study on a blood based real-time PCR for the diagnosis of acute schistosomiasis. BMC Infect Dis 13: 1–7.
-
14.
Ibironke O, Koukounari A, Asaolu S, Moustaki I, Shiff C, 2012. Validation of a new test for Schistosoma haematobium based on detection of Dra1 DNA fragments in urine: evaluation through latent class analysis. PLoS Negl Trop Dis 6: e1464.
-
15.
Van Riet E, Adegnika AA, Retra K, Vieira R, Tielens AGM, Lell B, Yazdanbakhsh M, 2007. Cellular and humoral responses to influenza in gabonese children living in rural and semi-urban areas. J Infect Dis 196: 1671–1678.
-
16.
Obeng BB, Aryeetey YA, de Dood CJ, Amoah AS, Larbi IA, Deelder AM, van Lieshout L, 2008. Application of a circulating-cathodic-antigen (CCA) strip test and real-time PCR, in comparison with microscopy, for the detection of Schistosoma haematobium in urine samples from Ghana. Ann Trop Med Parasitol 102: 625–633.
-
17.
Niesters HG, 2002. Clinical virology in real time. J Clin Virol 25: 3–12.
-
18.
Fernández-Soto P, Velasco Tirado V, Carranza Rodríguez C, Pérez-Arellano JL, Muro A, 2013. Long-term frozen storage of urine samples: a trouble to get PCR results in Schistosoma spp. DNA detection? PLoS ONE 8: e61703.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 41 | 41 | 5 |
| Full Text Views | 259 | 73 | 1 |
| PDF Downloads | 86 | 21 | 1 |