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Impact of Short-Time Urine Freezing on the Sensitivity of an Established Schistosoma Real-Time PCR Assay
Urogenital schistosomiaisis is a serious public health problem in sub-Saharan Africa. In this study, we have updated an established real-time polymerase chain reaction (PCR) routinely used in our laboratory. Schistosoma genus-specific real-time PCR was performed on DNA isolated from 85 urine samples and pellets obtained after centrifugation without and after frozen storage. The results revealed that concentration by centrifugation of the urine samples and freezing of the samples before extracting DNA improves the sensitivity of the PCR.
Author Notes
Financial support: EDCTP Senior Fellowship TA 11 40200025 Deutsche Forschungsgemeinschaft-funded project Deutsch-Afrikanische Kooperationsprojekte in der Infektiologie (DFG-Projekt KR 1150/6-1), EU-funded project Immunological Interplay between Poverty Related Diseases and Helminth infections: An African-European Research Initiative “IDEA” (HEALTH-F3-2009-241642). EU-Funded projects: The targeted development of a new generation Vaccine for Schistosomiasis “TheSchistoVac” (Health-2009-242107).
Authors' addresses: Hilaire M. Kenguele, Département de Biologie, Université des Sciences et Techniques de Masuku (USTM), Franceville, Gabon, E-mail: hkenguele@gmail.com. Ayola A. Adegnika, Anne-Marie Nkoma, Ulysse Ateba-Ngoa, Mirabeau Mbong, Jeannot Zinsou, and Bertrand Lell, Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Hôpital Albert Schweitzer Gabon, Institut für Tropenmedizin, Universität Tübingen, Tübingen, Germany, Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands, E-mails: aadegnika@gmail.com, mymhankoma@yahoo.fr, ulyssus7000@gmail.com, mirabs2006@yahoo.com, zinaff@gmail.com, and Bertrand.lell@gmail.com. Jaco J. Verweij, Laboratory for Medical Microbiology and Immunology, St. Elisabeth Hospital, Tilburg, The Netherlands, E-mail: j.verweij@elisabeth.nl.