Author:
- Introduction
- Methods
- Statistical analysis.
- Ethical aspects.
- Ethical aspects of animal experiments.
- Methods of laboratory diagnosis.
- Parasitological methods.
- Laboratory maintenance of the S. mansoni experimental cycle.
- Periovular reaction.
- Detection of IgM antibodies against antigens of the S. mansoni digestive tract.
- Antibody detection of S. mansoni egg antigens.
- Results
- Discussion
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
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Detection of Schistosoma mansoni Antibodies in a Low-Endemicity Area Using Indirect Immunofluorescence and Circumoval Precipitin Test
Maria Cristina Carvalho do Espírito-Santo
Maria Cristina Carvalho do Espírito-Santo
Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil
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Pedro Luiz Pinto
Pedro Luiz Pinto
Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil
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Cybele Gargioni
Cybele Gargioni
Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil
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Monica Viviana Alvarado-Mora
Monica Viviana Alvarado-Mora
Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil
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Vera Lúcia Pagliusi Castilho
Vera Lúcia Pagliusi Castilho
Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil
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João Ranato Rebello Pinho
João Ranato Rebello Pinho
Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil
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Expedito José de Albuquerque Luna
Expedito José de Albuquerque Luna
Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil
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Ronaldo Cesar Borges Gryschek
Ronaldo Cesar Borges Gryschek
Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil
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Parasitological diagnostic methods for schistosomiasis lack sensitivity, especially in regions of low endemicity. The objective of this study was to determine the prevalence of Schistosoma mansoni infections by antibody detection using the indirect immunofluorescence assay (IFA-IgM) and circumoval precipitin test (COPT). Serum samples of 572 individuals were randomly selected. The IFA-IgM and COPT were used to detect anti-S. mansoni antibodies. Of the patients studied, 15.9% (N = 91) were IFA-IgM positive and 5.1% (N = 29) had COPT reactions (P < 0.001 by McNemar's test). Immunodiagnostic techniques showed higher infection prevalence than had been previously estimated. This study suggests that combined use of these diagnostic tools could be useful for the diagnosis of schistosomiasis in epidemiological studies in areas of low endemicity.
Author Notes
Authors' addresses: Maria Cristina Espírito-Santo, Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil, E-mail: cristinasanto@usp.br. Pedro Luiz Pinto, Adolfo Lutz Institut, Department of Enteroparasites at the Parasitology, São Paulo, São Paulo, Brazil, E-mail: pedrp.luiz44@terra.com.br. Cybele Gargioni, Adolfo Lutz Institiute, Department of Enteroparasites at the Parasitology and Mycology, São Paulo, São Paulo, Brazil, E-mail: cgargioni@gmail.com. Mónica Viviana Alvarado-Mora, School of Medicine, University of São Paulo, Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, São Paulo, São Paulo, Brazil, E-mail: monica.viviana@usp.br. Vera Lúcia Pagliusi Castilho, Faculdade de Medicina da Universidade de São Paulo, Laboratory of Clinical Parasitology of the Central Laboratory of the Hosiital das Clinicas, São Paulo, São Paulo, Brazil, E-mail: vera.castilho@hc.fm.usp.br. João Ranato Rebello Pinho, Faculdade de Medicina da Universidade de São Paulo, Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, São Paulo, São Paulo, Brazil, E-mail: jrrpinho@usp.br. Expedito José de Albuquerque Luna, Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo, São Paulo, São Paulo, Brazil, E-mail: eluna@usp.br. Ronaldo Cesar Borges Gryschek, Faculdade de Medicina da Universidade de São Paulo, Department of Infectious and Parasitic Diseases, São Paulo, São Paulo, Brazil, E-mail: rebgry@usp.br.
- Introduction
- Methods
- Statistical analysis.
- Ethical aspects.
- Ethical aspects of animal experiments.
- Methods of laboratory diagnosis.
- Parasitological methods.
- Laboratory maintenance of the S. mansoni experimental cycle.
- Periovular reaction.
- Detection of IgM antibodies against antigens of the S. mansoni digestive tract.
- Antibody detection of S. mansoni egg antigens.
- Results
- Discussion
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
World Health Organization, 2001. Prevention and Control of Schistosomiasis and the Soil-Transmitted Helminthiasis. Report of a WHO Expert Committee. Geneva: WHO, 73.
-
2.
Chitsulo L, Engels D, Montresor A, Savioli L, 2000. The global status of schistosomiasis and its control. Acta Trop 77: 41–51.
-
3.
World Health Organization, 2001. Report of Informal Consultation on Schistosomiasis in Low Transmission Areas: Control Strategies and Criteria for Elimination. London, 10–13 April 2000. Geneva: WHO, 52.
-
4.
Alarcón de Noya BA, Guevara RR, Colmenares C, Losada S, Noya O, 2006. Low transmission areas of schistosomiasis in Venezuela: consequences on the diagnosis, treatment, and control. Mem Inst Oswaldo Cruz 101: 29–35.
-
5.
Ministério da Saúde, 2012. Secretaria de Vigilância a Saúde. Sistema de Informação do Programa de Controle da Esquistossomose. Sistema de Informação de Agravos em Saúde/2012. Casos confirmados de Esquistossomose, Brasil, Grandes Regiões e Unidades Federadas, 1995 a 2011. Brasil. (Dados preliminares, sujeitos à revisão).
-
6.
São Paulo, 2007. Secretaria de Estado da Saúde. Centro de Vigilância Epidemiológica. Coordenadoria de Controle de Doenças. Vigilância Epidemiológica e Controle da Esquistossomose-Normas e Instruções. São Paulo, 45.
-
7.
Katz N, Chaves A, Pellegrino J, 1972. A simple device for quantitative stool thick smear technique in schistosomiasis mansoni. Rev Inst Med Trop Sao Paulo 14: 397–400.
-
8.
Kongs A, Marks G, Verle P, Van der Stuyft P, 2001. The unreliability of the Kato-Katz technique limits its usefulness for evaluating S. mansoni infections. Trop Med Int Health 6: 163–169.
-
9.
Engels D, Sinzinkayo E, Gryseels B, 1996. Day-to-day egg count fluctuation in Schistosoma mansoni infection and its operational implications. Am J Trop Med Hyg 54: 319–324.
-
10.
Teesdale CH, Fahringer K, Chistulo L, 1985. Egg count variation and sensitivity of a thin smear technique for the diagnosis of Schistosoma mansoni. Trans R Soc Trop Med Hyg 79: 369–373.
-
11.
World Health Organization, 2012. Schistosomiasis. Fact Sheet No. 115. Geneva: WHO.
-
12.
Teles HM, de Carvalho ME, Santos Ferreira C, Zacharias F, de Lima VR, Fadel ML, 2002. Schistosomiasis mansoni in Bananal (State of São Paulo, Brazil): I. Efficiency of diagnostic and treatment procedures. Mem Inst Oswaldo Cruz 97: 181–186.
-
13.
Alarcón de Noya BA, Noya O, Balzan C, Cesari IM, 1992. New approaches for the control and eradication of schistosomiasis in Venezuela. Mem Inst Oswaldo Cruz 87: 227–231.
-
14.
Wang W, Li Y, Li H, Xing Y, Qu G, Dai J, Liang Y, 2012. Immunodiagnostic efficacy of detection of Schistosoma japonicum human infections in China: a meta analysis. Asian Pac J Trop Med 5: 15–23.
-
15.
Kanamura HY, Dias LC, da Silva RM, Glasser CM, Patucci RM, Vellosa SA, Antunes JL, 1998. A comparative epidemiologic study of specific antibodies (IgM and IgA) and parasitological findings in an endemic area of low transmission of Schistosoma mansoni. Rev Inst Med Trop Sao Paulo 40: 85–91.
-
16.
Burlandy-Soares LC, de Souza Dias LC, Kanamura HY, de Oliveira EJ, Ciaravolo RM, 2003. Schistosomiasis mansoni: follow-up of control program based on parasitologic and serologic methods in a Brazilian community of low endemicity. Mem Inst Oswaldo Cruz 98: 853–859.
-
17.
Oliver-Gonzalez J, 1954. Anti-egg precipitins in the serum of humans infected with Schistosoma mansoni. J Infect Dis 95: 86–91.
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