Genetic Structure Inferred from Mitochondrial 12S Ribosomal RNA Sequence of Oncomelania quadrasi, the Intermediate Snail Host of Schistosoma japonicum in the Philippines

Weerachai Saijuntha Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham, Thailand; Division of Environmental Health Sciences, Kochi Medical School, Nankoku, Japan; College of Public Health, University of the Philippines, Manila, Philippines; Municipal Health Office, Municipality of Gonzaga, Cagayan, Philippines; Department of Parasitology, and Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Ecology and Parasitology, Karlsruhe Institute of Technology, Karlsruhe, Germany; Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan

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Blanca Jarilla Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham, Thailand; Division of Environmental Health Sciences, Kochi Medical School, Nankoku, Japan; College of Public Health, University of the Philippines, Manila, Philippines; Municipal Health Office, Municipality of Gonzaga, Cagayan, Philippines; Department of Parasitology, and Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Ecology and Parasitology, Karlsruhe Institute of Technology, Karlsruhe, Germany; Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan

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Alvin K. Leonardo Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham, Thailand; Division of Environmental Health Sciences, Kochi Medical School, Nankoku, Japan; College of Public Health, University of the Philippines, Manila, Philippines; Municipal Health Office, Municipality of Gonzaga, Cagayan, Philippines; Department of Parasitology, and Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Ecology and Parasitology, Karlsruhe Institute of Technology, Karlsruhe, Germany; Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan

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Louie S. Sunico Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham, Thailand; Division of Environmental Health Sciences, Kochi Medical School, Nankoku, Japan; College of Public Health, University of the Philippines, Manila, Philippines; Municipal Health Office, Municipality of Gonzaga, Cagayan, Philippines; Department of Parasitology, and Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Ecology and Parasitology, Karlsruhe Institute of Technology, Karlsruhe, Germany; Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan

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Lydia R. Leonardo Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham, Thailand; Division of Environmental Health Sciences, Kochi Medical School, Nankoku, Japan; College of Public Health, University of the Philippines, Manila, Philippines; Municipal Health Office, Municipality of Gonzaga, Cagayan, Philippines; Department of Parasitology, and Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Ecology and Parasitology, Karlsruhe Institute of Technology, Karlsruhe, Germany; Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan

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Ross H. Andrews Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham, Thailand; Division of Environmental Health Sciences, Kochi Medical School, Nankoku, Japan; College of Public Health, University of the Philippines, Manila, Philippines; Municipal Health Office, Municipality of Gonzaga, Cagayan, Philippines; Department of Parasitology, and Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Ecology and Parasitology, Karlsruhe Institute of Technology, Karlsruhe, Germany; Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan

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Paiboon Sithithaworn Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham, Thailand; Division of Environmental Health Sciences, Kochi Medical School, Nankoku, Japan; College of Public Health, University of the Philippines, Manila, Philippines; Municipal Health Office, Municipality of Gonzaga, Cagayan, Philippines; Department of Parasitology, and Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Ecology and Parasitology, Karlsruhe Institute of Technology, Karlsruhe, Germany; Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan

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Trevor N. Petney Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham, Thailand; Division of Environmental Health Sciences, Kochi Medical School, Nankoku, Japan; College of Public Health, University of the Philippines, Manila, Philippines; Municipal Health Office, Municipality of Gonzaga, Cagayan, Philippines; Department of Parasitology, and Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Ecology and Parasitology, Karlsruhe Institute of Technology, Karlsruhe, Germany; Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan

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Masashi Kirinoki Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham, Thailand; Division of Environmental Health Sciences, Kochi Medical School, Nankoku, Japan; College of Public Health, University of the Philippines, Manila, Philippines; Municipal Health Office, Municipality of Gonzaga, Cagayan, Philippines; Department of Parasitology, and Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Ecology and Parasitology, Karlsruhe Institute of Technology, Karlsruhe, Germany; Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan

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Naoko Kato-Hayashi Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham, Thailand; Division of Environmental Health Sciences, Kochi Medical School, Nankoku, Japan; College of Public Health, University of the Philippines, Manila, Philippines; Municipal Health Office, Municipality of Gonzaga, Cagayan, Philippines; Department of Parasitology, and Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Ecology and Parasitology, Karlsruhe Institute of Technology, Karlsruhe, Germany; Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan

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Mihoko Kikuchi Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham, Thailand; Division of Environmental Health Sciences, Kochi Medical School, Nankoku, Japan; College of Public Health, University of the Philippines, Manila, Philippines; Municipal Health Office, Municipality of Gonzaga, Cagayan, Philippines; Department of Parasitology, and Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Ecology and Parasitology, Karlsruhe Institute of Technology, Karlsruhe, Germany; Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan

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Yuichi Chigusa Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham, Thailand; Division of Environmental Health Sciences, Kochi Medical School, Nankoku, Japan; College of Public Health, University of the Philippines, Manila, Philippines; Municipal Health Office, Municipality of Gonzaga, Cagayan, Philippines; Department of Parasitology, and Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Ecology and Parasitology, Karlsruhe Institute of Technology, Karlsruhe, Germany; Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan

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Takeshi Agatsuma Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham, Thailand; Division of Environmental Health Sciences, Kochi Medical School, Nankoku, Japan; College of Public Health, University of the Philippines, Manila, Philippines; Municipal Health Office, Municipality of Gonzaga, Cagayan, Philippines; Department of Parasitology, and Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Ecology and Parasitology, Karlsruhe Institute of Technology, Karlsruhe, Germany; Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan; Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan

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DOI:
https://doi.org/10.4269/ajtmh.13-0260
Volume/Issue:
Volume 90: Issue 6
Page(s):
1140–1145
Restricted access
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Species and subspecies of the Oncomelania hupensis species complex are recognized as intermediate hosts of Schistosoma japonicum. Of these species and subspecies, O. quadrasi is distributed throughout the Philippines. This study used 12S ribosomal RNA sequences to explore the genetic structure of O. quadrasi populations in the Philippines. Three subspecies, O. h. hupensis, O. h. formosana, and O. h. chiui of this group were also examined. The phylogenetic tree and haplotypes network showed that O. quadrasi separated from the subspecies. Ten O. quadrasi haplotypes (Oq1–Oq10) clustered in relation to their geographic origin. Genetic differentiation (FST) and estimated gene flow (Nm) among populations showed significant differences, ranging from 0.556–1.000 to 0.00–0.74, respectively. Genetic differences among groups (FCT = 0.466), populations within a group (FSC = 0.727), and populations (FST = 0.854) were observed. These results indicate that the O. quadrasi populations in the Philippines have a substructure associated with their geographic origin.

Author Notes

* Address correspondence to Takeshi Agatsuma, Division of Environmental Health Sciences, Kochi Medical School, Oko, Nankoku 783-8505, Japan. E-mail: agatsuma@kochi-u.ac.jp

Financial support: This study was supported by the Japan Society for the Promotion of Science (grant no. L-11566) and TRF-CHE-MSU (grant no. MRG5480009) to Weerachai Saijuntha.

Authors' addresses: Weerachai Saijuntha, Walai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham, Thailand, E-mail: weerachai.s@msu.ac.th. Blanca Jarilla and Takeshi Agatsuma, Division of Environmental Health Sciences, Kochi Medical School, Nankoku, Japan, E-mails: blancajarilla@yahoo.com and agatsuma@kochi-u.ac.jp. Alvin K. Leonardo and Lydia R. Leonardo, College of Public Health, University of the Philippines, Manila, Philippines, E-mail: leonardolydia7@gmail.com. Louie S. Sunico, Municipal Health Office, Municipality of Gonzaga, Cagayan, Philippines, E-mail: HRMO_gonzaga@yahoo.com. Ross H. Andrews and Paiboon Sithithaworn, Department of Parasitology, and Liver Fluke and Cholangiocarcinoma Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, E-mails: rhandrews@gmail.com and paib_sit@hotmail.com. Trevor N. Petney, Department of Ecology and Parasitology, Karlsruhe Institute of Technology, Karlsruhe, Germany, E-mail: petney@kit.edu. Masashi Kirinoki, Naoko Kato-Hayashi, and Yuichi Chigusa, Laboratory of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan, E-mails: kirinoki@dokkyomed.ac.jp, nkato@dokkyo.med.ac.jp, and ychigusa@dokkyomed.ac.jp. Mihoko Kikuchi, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan, E-mail: mkikuchi@nagasaki-u.ac.jp.

Copyright:
© The American Society of Tropical Medicine and Hygiene 2014
Received:
14 May 2013
|
Accepted:
12 Jan 2014
|
Published Online:
04 Jun 2014
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Online ISSN:
1476-1645
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