ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Desjeux P, 2004. Leishmaniasis: current situation and new perspectives. Comp Immunol Microbiol Infect Dis 27: 305–318.
-
2.
Chappuis F, Sundar S, Hailu A, Ghalib H, Rijal S, Peeling RW, Alvar J, Boelaert M, 2007. Visceral leishmaniasis: what are the needs for diagnosis, treatment and control? Nat Rev Microbiol 5: 873–882.
-
3.
Zijlstra EE, Ali MS, el-Hassan AM, el-Toum IA, Satti M, Ghalib HW, Kager PA, 1992. Kala-azar: a comparative study of parasitological methods and the direct agglutination test in diagnosis. Trans R Soc Trop Med Hyg 86: 505–507.
-
4.
Gatti S, Gramegna M, Klersy C, Madama S, Bruno A, Maserati R, Bernuzzi AM, Cevini C, Scaglia M, 2004. Diagnosis of visceral leishmaniasis: the sensitivities and specificities of traditional methods and a nested PCR assay. Ann Trop Med Parasitol 98: 667–676.
-
5.
Antinori S, Calattini S, Longhi E, Bestetti G, Piolini R, Magni C, Orlando G, Gramiccia M, Acquaviva V, Foschi A, Corvasce S, Colomba C, Titone L, Parravicini C, Cascio A, Corbellino M, 2007. Clinical use of polymerase chain reaction performed on peripheral blood and bone marrow samples for the diagnosis and monitoring of visceral leishmaniasis in HIV-infected and HIV-uninfected patients: a single-center, 8-year experience in Italy and review of the literature. Clin Infect Dis 44: 1602–1610.
-
6.
Pandey K, Pant S, Kanbara H, Shuaibu MN, Mallik AK, Pandey BD, Kaneko O, Yanagi T, 2008. Molecular detection of Leishmania parasites from whole bodies of sandflies collected in Nepal. Parasitol Res 103: 293–297.
-
7.
Aransay AM, Scoulica E, Tselentis Y, 2000. Detection and identification of Leishmania DNA within naturally infected sand flies by seminested PCR on minicircle kinetoplastic DNA. Appl Environ Microbiol 66: 1933–1938.
-
8.
Srivastava P, Mehrotra S, Tiwary P, Chakravarty J, Sundar S, 2011. Diagnosis of Indian visceral leishmaniasis by nucleic acid detection using PCR. PLoS One 6: e19304.
-
9.
Chargui N, Haouas N, Jaouadi K, Gorcii M, Pratlong F, Dedet JP, Mezhoud H, Babba H, 2012. Usefulness of a PCR-based method in the detection and species identification of Leishmania from clinical samples. Pathol Biol (Paris) 60: e75–e79.
-
10.
Salam MA, Khan MG, Bhaskar KR, Afrad MH, Huda MM, Mondal D, 2012. Peripheral blood buffy coat smear: a promising tool for diagnosis of visceral leishmaniasis. J Clin Microbiol 50: 837–840.
-
11.
Kent RJ, Norris DE, 2005. Identification of mammalian blood meals in mosquitoes by a multiplexed polymerase chain reaction targeting cytochrome B. Am J Trop Med Hyg 73: 336–342.
-
12.
Edgar RC, 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32: 1792–1797.
-
13.
Brandonisio O, Fumarola L, Maggi P, Cavaliere R, Spinelli R, Pastore G, 2002. Evaluation of a rapid immunochromatographic test for serodiagnosis of visceral leishmaniasis. Eur J Clin Microbiol Infect Dis 21: 461–464.
-
14.
Sundar S, Pai K, Sahu M, Kumar V, Murray HW, 2002. Immunochromatographic strip-test detection of anti-K39 antibody in Indian visceral leishmaniasis. Ann Trop Med Parasitol 96: 19–23.
-
15.
Carvalho SF, Lemos EM, Corey R, Dietze R, 2003. Performance of recombinant K39 antigen in the diagnosis of Brazilian visceral leishmaniasis. Am J Trop Med Hyg 68: 321–324.
-
16.
Bern C, Jha SN, Joshi AB, Thakur GD, Bista MB, 2000. Use of the recombinant K39 dipstick test and the direct agglutination test in a setting endemic for visceral leishmaniasis in Nepal. Am J Trop Med Hyg 63: 153–157.
-
17.
Sarker CB, Momen A, Jamal MF, Siddiqui NI, Siddiqui FM, Chowdhury KS, Rahman S, Talukder SI, 2003. Immunochromatographic (rK39) strip test in the diagnosis of visceral leishmaniasis in Bangladesh. Mymensingh Med J 12: 93–97.
-
18.
Zijlstra EE, Nur Y, Desjeux P, Khalil EA, El-Hassan AM, Groen J, 2001. Diagnosing visceral leishmaniasis with the recombinant K39 strip test: experience from the Sudan. Trop Med Int Health 6: 108–113.
-
19.
Boelaert M, Rijal S, Regmi S, Singh R, Karki B, Jacquet D, Chappuis F, Campino L, Desjeux P, Le Ray D, Koirala S, Van der Stuyft P, 2004. A comparative study of the effectiveness of diagnostic tests for visceral leishmaniasis. Am J Trop Med Hyg 70: 72–77.
-
20.
Sundar S, Reed SG, Singh VP, Kumar PC, Murray HW, 1998. Rapid accurate field diagnosis of Indian visceral leishmaniasis. Lancet 351: 563–565.
-
21.
WHO, 2011. Visceral Leishmaniasis Rapid Diagnostic Test Performance. Geneva: World Health Organization.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 102 | 73 | 12 |
| Full Text Views | 396 | 11 | 2 |
| PDF Downloads | 71 | 8 | 2 |
A New Molecular Surveillance System for Leishmaniasis
Kishor Pandey
Kishor Pandey
Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, Nepal; Janakpur Zonal Hospital, Janakpur, Nepal; Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden; Nanyang Technological University, School of Biological Sciences, Singapore
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Basu Dev Pandey
Basu Dev Pandey
Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, Nepal; Janakpur Zonal Hospital, Janakpur, Nepal; Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden; Nanyang Technological University, School of Biological Sciences, Singapore
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Arun Kumar Mallik
Arun Kumar Mallik
Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, Nepal; Janakpur Zonal Hospital, Janakpur, Nepal; Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden; Nanyang Technological University, School of Biological Sciences, Singapore
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Jyoti Acharya
Jyoti Acharya
Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, Nepal; Janakpur Zonal Hospital, Janakpur, Nepal; Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden; Nanyang Technological University, School of Biological Sciences, Singapore
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Kentaro Kato
Kentaro Kato
Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, Nepal; Janakpur Zonal Hospital, Janakpur, Nepal; Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden; Nanyang Technological University, School of Biological Sciences, Singapore
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Osamu Kaneko
Osamu Kaneko
Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, Nepal; Janakpur Zonal Hospital, Janakpur, Nepal; Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden; Nanyang Technological University, School of Biological Sciences, Singapore
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Pedro Eduardo Ferreira
Pedro Eduardo Ferreira
Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, Nepal; Janakpur Zonal Hospital, Janakpur, Nepal; Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan; Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden; Nanyang Technological University, School of Biological Sciences, Singapore
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Presently, global efforts are being made to control and eradicate the deadliest tropical diseases through the improvement of adequate interventions. A critical point for programs to succeed is the prompt and accurate diagnosis in endemic regions. Rapid diagnostic tests (RDTs) are being massively deployed and used to improve diagnosis in tropical countries. In the present report, we evaluated the hypothesis of, after use for diagnosis, the reuse of the Leishmania RDT kit as a DNA source, which can be used downstream as a molecular surveillance and/or quality control tool. As a proof of principle, a polymerase chain reaction-based method was used to detect Leishmania spp. minicircle kinetoplast DNA from leishmaniasis RDT kits. Our results show that Leishmania spp. DNA can be extracted from used RDTs and may constitute an important, reliable, and affordable tool to assist in future leishmaniasis molecular surveillance methods.
Author Notes
Financial support: This work is supported by UBS (Union Bank of Switzerland) Optimus Foundation and Karolinska Institute Research Foundations.
Authors' addresses: Kishor Pandey and Osamu Kaneko, Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan, E-mails: pandey_kishor@hotmail.com and okaneko@nagasaki-u.ac.jp. Basu Dev Pandey and Jyoti Acharya, Infectious Disease, Sukraraj Tropical and Infectious Disease Hospital, Kathmandu, Nepal, E-mails: drbasupandey@gmail.com and acharyajyoti30@yahoo.com. Arun Kumar Mallik, Infectious Disease, Janakpur Zonal Hospital, Janakpur, Nepal, E-mail: ak_mallik@yahoo.com. Kentaro Kato, Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki, Japan, E-mail: katoken@nagasaki-u.ac.jp. Pedro Eduardo Ferreira, Nanyang Technological University, School of Biological Sciences, Singapore, E-mail: pedro.ferreira@ki.se.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Desjeux P, 2004. Leishmaniasis: current situation and new perspectives. Comp Immunol Microbiol Infect Dis 27: 305–318.
-
2.
Chappuis F, Sundar S, Hailu A, Ghalib H, Rijal S, Peeling RW, Alvar J, Boelaert M, 2007. Visceral leishmaniasis: what are the needs for diagnosis, treatment and control? Nat Rev Microbiol 5: 873–882.
-
3.
Zijlstra EE, Ali MS, el-Hassan AM, el-Toum IA, Satti M, Ghalib HW, Kager PA, 1992. Kala-azar: a comparative study of parasitological methods and the direct agglutination test in diagnosis. Trans R Soc Trop Med Hyg 86: 505–507.
-
4.
Gatti S, Gramegna M, Klersy C, Madama S, Bruno A, Maserati R, Bernuzzi AM, Cevini C, Scaglia M, 2004. Diagnosis of visceral leishmaniasis: the sensitivities and specificities of traditional methods and a nested PCR assay. Ann Trop Med Parasitol 98: 667–676.
-
5.
Antinori S, Calattini S, Longhi E, Bestetti G, Piolini R, Magni C, Orlando G, Gramiccia M, Acquaviva V, Foschi A, Corvasce S, Colomba C, Titone L, Parravicini C, Cascio A, Corbellino M, 2007. Clinical use of polymerase chain reaction performed on peripheral blood and bone marrow samples for the diagnosis and monitoring of visceral leishmaniasis in HIV-infected and HIV-uninfected patients: a single-center, 8-year experience in Italy and review of the literature. Clin Infect Dis 44: 1602–1610.
-
6.
Pandey K, Pant S, Kanbara H, Shuaibu MN, Mallik AK, Pandey BD, Kaneko O, Yanagi T, 2008. Molecular detection of Leishmania parasites from whole bodies of sandflies collected in Nepal. Parasitol Res 103: 293–297.
-
7.
Aransay AM, Scoulica E, Tselentis Y, 2000. Detection and identification of Leishmania DNA within naturally infected sand flies by seminested PCR on minicircle kinetoplastic DNA. Appl Environ Microbiol 66: 1933–1938.
-
8.
Srivastava P, Mehrotra S, Tiwary P, Chakravarty J, Sundar S, 2011. Diagnosis of Indian visceral leishmaniasis by nucleic acid detection using PCR. PLoS One 6: e19304.
-
9.
Chargui N, Haouas N, Jaouadi K, Gorcii M, Pratlong F, Dedet JP, Mezhoud H, Babba H, 2012. Usefulness of a PCR-based method in the detection and species identification of Leishmania from clinical samples. Pathol Biol (Paris) 60: e75–e79.
-
10.
Salam MA, Khan MG, Bhaskar KR, Afrad MH, Huda MM, Mondal D, 2012. Peripheral blood buffy coat smear: a promising tool for diagnosis of visceral leishmaniasis. J Clin Microbiol 50: 837–840.
-
11.
Kent RJ, Norris DE, 2005. Identification of mammalian blood meals in mosquitoes by a multiplexed polymerase chain reaction targeting cytochrome B. Am J Trop Med Hyg 73: 336–342.
-
12.
Edgar RC, 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32: 1792–1797.
-
13.
Brandonisio O, Fumarola L, Maggi P, Cavaliere R, Spinelli R, Pastore G, 2002. Evaluation of a rapid immunochromatographic test for serodiagnosis of visceral leishmaniasis. Eur J Clin Microbiol Infect Dis 21: 461–464.
-
14.
Sundar S, Pai K, Sahu M, Kumar V, Murray HW, 2002. Immunochromatographic strip-test detection of anti-K39 antibody in Indian visceral leishmaniasis. Ann Trop Med Parasitol 96: 19–23.
-
15.
Carvalho SF, Lemos EM, Corey R, Dietze R, 2003. Performance of recombinant K39 antigen in the diagnosis of Brazilian visceral leishmaniasis. Am J Trop Med Hyg 68: 321–324.
-
16.
Bern C, Jha SN, Joshi AB, Thakur GD, Bista MB, 2000. Use of the recombinant K39 dipstick test and the direct agglutination test in a setting endemic for visceral leishmaniasis in Nepal. Am J Trop Med Hyg 63: 153–157.
-
17.
Sarker CB, Momen A, Jamal MF, Siddiqui NI, Siddiqui FM, Chowdhury KS, Rahman S, Talukder SI, 2003. Immunochromatographic (rK39) strip test in the diagnosis of visceral leishmaniasis in Bangladesh. Mymensingh Med J 12: 93–97.
-
18.
Zijlstra EE, Nur Y, Desjeux P, Khalil EA, El-Hassan AM, Groen J, 2001. Diagnosing visceral leishmaniasis with the recombinant K39 strip test: experience from the Sudan. Trop Med Int Health 6: 108–113.
-
19.
Boelaert M, Rijal S, Regmi S, Singh R, Karki B, Jacquet D, Chappuis F, Campino L, Desjeux P, Le Ray D, Koirala S, Van der Stuyft P, 2004. A comparative study of the effectiveness of diagnostic tests for visceral leishmaniasis. Am J Trop Med Hyg 70: 72–77.
-
20.
Sundar S, Reed SG, Singh VP, Kumar PC, Murray HW, 1998. Rapid accurate field diagnosis of Indian visceral leishmaniasis. Lancet 351: 563–565.
-
21.
WHO, 2011. Visceral Leishmaniasis Rapid Diagnostic Test Performance. Geneva: World Health Organization.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 102 | 73 | 12 |
| Full Text Views | 396 | 11 | 2 |
| PDF Downloads | 71 | 8 | 2 |