Evaluation of a Latex Agglutination Assay for the Identification of Burkholderia pseudomallei and Burkholderia mallei

Brea D. Duval Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand

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Mindy G. Elrod Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand

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Jay E. Gee Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand

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Narisara Chantratita Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand

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Sarunporn Tandhavanant Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand

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Direk Limmathurotsakul Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand

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Alex R. Hoffmaster Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand

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Cases of melioidosis and glanders are rare in the United States, but the etiologic agents of each disease (Burkholderia pseudomallei and Burkholderia mallei, respectively) are classified as Tier 1 select agents because of concerns about their potential use as bioterrorism agents. A rapid, highly sensitive, and portable assay for clinical laboratories and field use is required. Our laboratory has further evaluated a latex agglutination assay for its ability to identify B. pseudomallei and B. mallei isolates. This assay uses a monoclonal antibody that specifically recognizes the capsular polysaccharide produced by B. pseudomallei and B. mallei, but is absent in closely related Burkholderia species. A total of 110 B. pseudomallei and B. mallei were tested, and 36 closely related Burkholderia species. The latex agglutination assay was positive for 109 of 110 (99.1% sensitivity) B. pseudomallei and B. mallei isolates tested.

Author Notes

* Address correspondence to Alex R. Hoffmaster, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, MS-G34, Atlanta, GA 30333. E-mail: amh9@cdc.gov

Authors' addresses: Brea D. Duval, Mindy G. Elrod, Jay E. Gee, and Alex R. Hoffmaster, Centers for Disease Control and Prevention, Atlanta, GA, E-mails: BDuval@cdc.gov, MGElrod@cdc.gov, JGee1@cdc.gov, and AHoffmaster@cdc.gov. Narisara Chantratita, Sarunporn Tandhavanant, and Direk Limmathurotsakul, Mahidol University, Bangkok, Thailand, E-mails: Narisara@tropmedres.ac, Sarunporn@tropmedres.ac, and Direk@tropmedres.ac.

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