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Immune Responses to O-Specific Polysaccharide and Lipopolysaccharide of Vibrio cholerae O1 Ogawa in Adult Bangladeshi Recipients of an Oral Killed Cholera Vaccine and Comparison to Responses in Patients with Cholera

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  • Centre for Vaccine Sciences, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka, Bangladesh; NIDDK, LBC, National Institutes of Health, Bethesda, Maryland; Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts; Departments of Medicine and Pediatrics, Harvard Medical School, Boston, Massachusetts; Department of Biochemistry and Molecular Biology, University of Dhaka, Bangladesh; Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts; Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts

Protective immunity to cholera is serogroup specific, and serogrouping is defined by the O-specific polysaccharide (OSP) of lipopolysaccharide (LPS). We characterized OSP-specific immune responses in adult recipients of an oral killed cholera vaccine (OCV WC-rBS) and compared these with responses in patients with cholera caused by Vibrio cholerae O1 Ogawa. Although vaccinees developed plasma immunoglobulin G (IgG), IgM, IgA antibody and antibody secreting cell (ASC, marker of mucosal response) to Ogawa OSP and LPS 7 days after vaccination, responses were significantly lower than that which occurred after cholera. Similarly, patients recovering from cholera had detectable IgA, IgM, and IgG memory B cell (MBC) responses against OSP and LPS on Day 30 and Day 90, whereas vaccinees only developed IgG responses to OSP 30 days after the second immunization. The markedly lower ASC and MBC responses to OSP and LPS observed among vaccinees might explain, in part, the lower protection of an OCV compared with natural infection.

Author Notes

* Address correspondence to Firdausi Qadri, 68 Shaheed Tajuddin Ahmed Sharani, Mohakhali, Dhaka-1212, Bangladesh. E-mail: fqadri@icddrb.org

Financial support: This research was supported by the icddr,b, by the Intramural Research Program of the National Institutes of Health, NIDDK, and extramural grants from the National Institutes of Health, including the National Institute of Allergy and Infectious Diseases (U01 AI058935 [S.B.C., E.T.R.], R03 AI063079 [F.Q.], U01 AI077883 and AI106878 [E.T.R.]), K08 AI089721 [R.C.C.], K08AI100923 [D.T.L.]) and the Fogarty International Center, Training Grant in Vaccine Development and Public Health (TW005572 [T.U., M.M.A., R.R., and F.Q.]), Career Development Awards (K01 TW07409 [J.B.H.] and K01 TW07144 [R.C.L.], and a Fogarty International Clinical Research Scholars Award (R24 TW007988 [T.U., R.A.J.]), as well as by Swedish Sida grant INT-ICDDR,B-HN-01-AV (F.Q.), a Physician Scientist Early Career Award from the Howard Hughes Medical Institute (R.C.L.), a Postdoctoral Fellowship in Tropical Infectious Diseases from the American Society for Tropical Medicine and Hygiene - Burroughs Wellcome Fund (D.T.L.), and a Thrasher Research Fund Early Career Award (D.T.L.).

Authors' addresses: Taher Uddin, Amena Aktar, Russell A. Johnson, M. Arifur Rahman, Sadia Afrin, Aklima Akter, Mohammad Murshid Alam, Fahima Chowdhury, Ashraful I. Khan, and Firdausi Qadri, International Centre For Diarrhoeal Disease Research, Bangladesh (icddr,b) - Immunology Laboratory, Dhaka, Bangladesh, E-mails: taher_imm@icddrb.org, bio_amn015@yahoo.com, russell.a.johnson@gmail.com, marifur@icddrb.org, sadia_afrin89@yahoo.com, aklima17@gmail.com, shafiul@icddrb.org, fchowdhury@icddrb.org, ashrafk@icddrb.org, and fqadri@icddrb.org. Peng Xu and Pavol Kováč, National Institutes of Health - NIDDK, LBC, Bethesda, MD, E-mails: xup3@mail.nih.gov and Kovac@mail.nih.gov. Daniel T. Leung, Meagan K. Bufano, Yanan Yu, and Ying Wu-Freeman, Massachusetts General Hospital - Infectious Diseases, Boston, MA, E-mails: dleung@partners.org, MBUFANO@partners.org, YYVAUGHN@PARTNERS.ORG, and YWUFREEMAN@PARTNERS.ORG. Atiqur Rahman, University of Dhaka - Department of Biochemistry and Molecular Biology, Dhaka, Bangladesh, E-mail: atique303@gmail.com. Rasheduzzaman Rashu, International Centre For Diarrhoeal Disease Research, Bangladesh (icddr,b) - Immunology Laboratory, Dhaka, Bangladesh, and Massachusetts General Hospital - Infectious Diseases, Boston, MA, E-mail: rashedimm@yahoo.com. Jason B. Harris, Department of Pediatrics, Harvard Medical School, Boston, MA, and Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, E-mail: jbharris@partners.org. Regina C. LaRocque and Richelle C. Charles, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, and Department of Medicine, Harvard Medical School, Boston, MA, E-mails: RCLAROCQUE@PARTNERS.ORG and RCCHARLES@PARTNERS.ORG. Stephen Calderwood, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, Department of Medicine, Harvard Medical School, Boston, MA, and Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, E-mail: SCALDERWOOD@PARTNERS.ORG. Edward T. Ryan, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, Department of Medicine, Harvard Medical School, Boston, MA, and Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA, E-mail: ETRYAN@PARTNERS.ORG.

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