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Oxidative Stress Markers Correlate with Renal Dysfunction and Thrombocytopenia in Severe Leptospirosis

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  • Laboratory of Pathology and Molecular Biology, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil; Faculty of Medicine, Federal University of Bahia, Salvador, BA, Brazil; Institute of Collective Health, Federal University of Bahia, Salvador, BA, Brazil; Department of Epidemiology of Microbial Diseases, Yale School of Public Health New Haven, Connecticut

Leptospirosis is a zoonotic disease that causes severe manifestations such as Weil's disease and pulmonary hemorrhage syndrome. The aim of this study was to evaluate whether reactive oxygen species (ROS) production and antioxidant reduced glutathione (GSH) levels are related to complications in patients hospitalized with leptospirosis. The ROS production and GSH levels were measured in blood samples of 12 patients and nine healthy controls using chemiluminescence and absorbance assays. We found that ROS production was higher and GSH levels were lower in leptospirosis patients compared with healthy individuals. Among patients, GSH depletion was correlated with thrombocytopenia and elevated serum creatinine, whereas a strong positive correlation was observed between ROS production and elevated serum potassium. Additional investigation of the biological significance of ROS production and GSH levels is warranted as they may guide the development of novel adjuvant therapies for leptospirosis targeting oxidative stress.

Author Notes

* Address correspondence to Mitermayer Galvão Reis, Oswaldo Cruz Foundation, Rua Waldemar Falcão, 121, Candeal – Salvador/BA CEP: 40296-710. E-mail: miter@bahia.fiocruz.br† These authors contributed equally to this work.

Financial support: This work was supported by CNPq grant no. 558714/2008-0; Program of Nucleus of Excellency; Brazilian Research Council no. 020/2009; National Institutes of Health (U01 AI088752, R01 AI052473, R01 TW009504, and D43 TW00919).

Authors' addresses: Alan M. Araújo, Laboratory of Pathology and Molecular Biology, Oswaldo Cruz Foundation, Rua Waldemar Falcão, 121, Candeal ? Salvador Bahia Brazil 40296-710, E-mail: amoreiradearaujo@gmail.com. Eliana A. G. Reis, Laboratory of Pathology and Molecular Biology, Oswaldo Cruz Foundation, Rua Waldemar Falcão, 121, Candeal ? Salvador Bahia Brazil 40296-710, E-mail: eagreis@gmail.com. Daniel Abensur Athanazio, Faculty of Medicine, Federal University of Bahia Brazil, Praça XV de novembro, s/n - Largo do Terreiro de Jesus, 40025010, E-mail: daa@ufba.br. Guilherme S. Ribeiro, Institute of Collective Health, Federal University of Bahia, Salvador, Bahia, Brazil 40110-040, E-mail: gsribeiro@ufba.br. Jose Hagan, Yale University, New Haven, CT, and Oswaldo Cruz Foundation, Rua Waldemar Falcão, 121, Candeal - Salvador Bahia Brazil 40296-710, E-mail: jose.hagan@gmail.com. Guilherme C. Araujo, Laboratory of Pathology and Molecular Biology, Oswaldo Cruz Foundation, Rua Waldemar Falcão, 121, Candeal ? Salvador Bahia Brazil 40296-710, E-mail: guyaraujo@gmail.com. Alcineia O. Damião, Laboratory of Pathology and Molecular Biology, Oswaldo Cruz Foundation, Rua Waldemar Falcão, 121, Candeal ? Salvador Bahia Brazil 40296-710, E-mail: alcidamiao@gmail.com. Nicolli S. Couto, Laboratory of Pathology and Molecular Biology, Oswaldo Cruz Foundation, Rua Waldemar Falcão, 121, Candeal ? Salvador Bahia Brazil 40296-710, E-mail: nicolli.sorice@gmail.com. Albert I. Ko, Yale University, New Haven, CT, and Oswaldo Cruz Foundation, Rua Waldemar Falcão, 121, Candeal - Salvador Bahia Brazil 40296-710, E-mail: albert.ko@yale.edu. Alberto Noronha-Dutra, Oswaldo Cruz Foundation, Rua Waldemar Falcão, 121, Candeal ? Salvador Bahia Brazil 40296-710, E-mail: dutra.alberto@gmail.com. Mitermayer G. Reis, Laboratory of Pathology and Molecular Biology, Oswaldo Cruz Foundation, Rua Waldemar Falcão, 121, Candeal - Salvador/Bahia Brazil 40296-710, E-mail: miter@bahia.fiocruz.br.

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