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Longitudinal Evaluation of Enteric Protozoa in Haitian Children by Stool Exam and Multiplex Serologic Assay

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  • Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Division of Parasitic Diseases and Malaria, National Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia; Departments of Medicine, Microbiology, and Pathology, University of Virginia, and TECHLAB, Inc., Blacksburg, Virginia

Haitian children were monitored longitudinally in a filariasis study. Included were stool samples examined for Giardia intestinalis and Entamoeba histolytica cysts, and serum specimens analyzed for immunoglobulin G (IgG) responses to eight recombinant antigens from G. intestinalis (variant-specific surface protein [VSP1–VSP5]), E. histolytica (lectin adhesion molecule [LecA]), and Cryptosporidium parvum (17- and 27-kDa) using a multiplex bead assay. The IgG responses to VSP antigens peaked at 2 years of age and then diminished and were significantly lower (P < 0.002) in children > 4.5 years than in children < 4.5 years. The IgG responses to Cryptosporidium tended to increase with age. The IgG responses to LecA and VSP antigens and the prevalence of stools positive for cysts were significantly higher (P < 0.037 and P < 0.035, respectively) in the rainy season than in the dry season. The multiplex bead assay provides a powerful tool for analyzing serologic responses to multiple pathogens.

Author Notes

* Address correspondence to Delynn M. Moss, Centers for Disease Control and Prevention, Division of Foodborne, Waterborne, and Environmental Diseases, 1600 Clifton Rd., Atlanta, GA 30329. E-mail: dmm3@cdc.gov

Financial support: We appreciate the financial support of the National Institutes of Health (NIH), CDC, and the UNDP/World Bank/WHO Special Program for Research and Training in Tropical Diseases (grant nos. 920528 and 940441). Katy Hamlin was supported by a CDC/APHL Emerging Infectious Disease Fellowship. Support for the production and purification of the LecA antigen was provided by NIH, grant R01 AI043596.

Authors' addresses: Delynn M. Moss, Jeffrey W. Priest, and Gordana Derado, Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases, Waterborne Disease Prevention Branch, Atlanta, GA, E-mails: dmm3@cdc.gov, jip8@cdc.gov, and uwx8@cdc.gov. Katy Hamlin, The George Washington University School of Public Health and Health Sciences, Washington, DC, E-mail: kl.hamlin28@gmail.com. Joel Herbein, TechLab, Inc., Blacksburg, VA, E-mail: jherbein@techlab.com. William A. Petri Jr., University of Virginia, Health Sciences Center, Charlottesville, VA, E-mail: wap3q@virginia.edu. Patrick J. Lammie, Centers for Disease Control and Prevention, Division of Parasitic Diseases, Atlanta, GA, E-mail: pjl1@cdc.gov.

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