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Kinetics of Dengue Non-Structural Protein 1 Antigen and IgM and IgA Antibodies in Capillary Blood Samples from Confirmed Dengue Patients

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  • Laboratoire de Virologie, Centre National de Référence des Arbovirus, Laboratoire Associé, Région Antilles Guyane, Institut Pasteur de la Guyane, Cayenne, French Guiana; Far East Medical Vietnam Limited, Ho Chi Minh City, Vietnam; Pasteur Institute of Ho Chi Minh City, Ho Chi Minh City, Vietnam; Laboratoire des Interactions Virus-Hôtes, Institut Pasteur de la Guyane, Cayenne, French Guiana; Centre d'Epidémiologie et de Santé Publique des Armées, Marseille, France; Unité Mixte de Recherche Scientifique 872, Pôle 4, Equipe 16, Institut National de la Santé et de la Recherche Médicale, Centre de Recherches Biomédicales des Cordeliers, Paris, France

Large-scale epidemiological surveillance of dengue in the field and dengue patient management require simple methods for sample collection, storage, and transportation as well as effective diagnostic tools. We evaluated the kinetics of three biological markers of dengue infection—non-structural protein 1 (NS1) antigen, immunoglobulin M (IgM), and IgA—in sequential capillary blood samples collected from fingertips of confirmed dengue patients. The overall sensitivities and specificities of the tests were 96% and 100%, respectively, for NS1, 58.1% and 100%, respectively, for IgM, and 33% and 100%, respectively, for IgA. During the acute phase of the disease, NS1 was the best marker of dengue infection, with a sensitivity of 98.7%, whereas from day 5, all three markers exhibited relevant levels of sensitivity. This first descriptive study of the kinetics of biological markers of dengue in capillary blood samples confirms the usefulness of this biological compartment for dengue diagnosis and argues for its exploitation in community-level and remote settings.

Author Notes

* Address correspondence to Séverine Matheus, Laboratoire de Virologie, Centre National de Référence des Arbovirus, Laboratoire associé, Région Antilles Guyane, Institut Pasteur de la Guyane, 23 Avenue Pasteur, BP 6010, 97306 Cayenne Cedex, French Guiana. E-mail: smatheus@pasteur-cayenne.fr

Financial support: This study was supported by Conseil Régional de la Guyane Agreement 60/2007/CR.

Authors' addresses: Séverine Matheus and Bhetty Labeau, Laboratoire de Virologie, Centre National de Référence des Arbovirus, Laboratoire Associé, Institut Pasteur de la Guyane, Cayenne, French Guiana, E-mails: smatheus@pasteur-cayenne.fr and blabeau@pasteur-cayenne.fr. Thai Binh Pham, Far East Medical Vietnam Limited, Ho Chi Minh City, Vietnam, E-mail: binh.pham@fvhospital.com. Vu Thi Que Huong, Laboratoire de Virologie, Institut Pasteur Vietnam, Ho Chi Minh City, Vietnam, E-mail: huongpasteur@hotmail.com. Vincent Lacoste, Laboratoire des Interactions Virus-Hôtes, Institut Pasteur de la Guyane, Cayenne, French Guiana, E-mail: vlacoste@pasteur-cayenne.fr. Xavier Deparis, Centre d'Epidémiologie et de Santé Publique des Armées, Marseille, France, E-mail: xavier.deparis@wanadoo.fr. Vincent Marechal, UMRS 872, Pôle 4, Equipe 16, Institut National de la Santé et de la Recherche Médicale, Centre de Recherches Biomédicales des Cordeliers, Paris, France, E-mail: vincent.marechal@upmc.fr.

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