Pre-Existing Cross-Reactive Antibodies to Avian Influenza H5N1 and 2009 Pandemic H1N1 in US Military Personnel

Sathit Pichyangkul Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Department of Dentistry, Chulalongkorn University, Bangkok, Thailand; Hong Kong University—Pasteur Research Center, Hong Kong

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Somporn Krasaesub Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Department of Dentistry, Chulalongkorn University, Bangkok, Thailand; Hong Kong University—Pasteur Research Center, Hong Kong

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Anan Jongkaewwattana Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Department of Dentistry, Chulalongkorn University, Bangkok, Thailand; Hong Kong University—Pasteur Research Center, Hong Kong

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Arunee Thitithanyanont Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Department of Dentistry, Chulalongkorn University, Bangkok, Thailand; Hong Kong University—Pasteur Research Center, Hong Kong

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Suwimon Wiboon-ut Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Department of Dentistry, Chulalongkorn University, Bangkok, Thailand; Hong Kong University—Pasteur Research Center, Hong Kong

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Kosol Yongvanitchit Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Department of Dentistry, Chulalongkorn University, Bangkok, Thailand; Hong Kong University—Pasteur Research Center, Hong Kong

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Amporn Limsalakpetch Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Department of Dentistry, Chulalongkorn University, Bangkok, Thailand; Hong Kong University—Pasteur Research Center, Hong Kong

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Utaiwan Kum-Arb Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Department of Dentistry, Chulalongkorn University, Bangkok, Thailand; Hong Kong University—Pasteur Research Center, Hong Kong

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Duangrat Mongkolsirichaikul Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Department of Dentistry, Chulalongkorn University, Bangkok, Thailand; Hong Kong University—Pasteur Research Center, Hong Kong

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Nuanpan Khemnu Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Department of Dentistry, Chulalongkorn University, Bangkok, Thailand; Hong Kong University—Pasteur Research Center, Hong Kong

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Rangsini Mahanonda Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Department of Dentistry, Chulalongkorn University, Bangkok, Thailand; Hong Kong University—Pasteur Research Center, Hong Kong

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Jean-Michel Garcia Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Department of Dentistry, Chulalongkorn University, Bangkok, Thailand; Hong Kong University—Pasteur Research Center, Hong Kong

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Carl J. Mason Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Department of Dentistry, Chulalongkorn University, Bangkok, Thailand; Hong Kong University—Pasteur Research Center, Hong Kong

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Douglas S. Walsh Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Department of Dentistry, Chulalongkorn University, Bangkok, Thailand; Hong Kong University—Pasteur Research Center, Hong Kong

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David L. Saunders Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Department of Dentistry, Chulalongkorn University, Bangkok, Thailand; Hong Kong University—Pasteur Research Center, Hong Kong

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DOI:
https://doi.org/10.4269/ajtmh.13-0151
Volume/Issue:
Volume 90: Issue 1
Page(s):
149–152
Restricted access
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We studied cross-reactive antibodies against avian influenza H5N1 and 2009 pandemic (p) H1N1 in 200 serum samples from US military personnel collected before the H1N1 pandemic. Assays used to measure antibodies against viral proteins involved in protection included a hemagglutination inhibition (HI) assay and a neuraminidase inhibition (NI) assay. Viral neutralization by antibodies against avian influenza H5N1 and 2009 pH1N1 was assessed by influenza (H5) pseudotyped lentiviral particle-based and H1N1 microneutralization assays. Some US military personnel had cross-neutralizing antibodies against H5N1 (14%) and 2009 pH1N1 (16.5%). The odds of having cross-neutralizing antibodies against 2009 pH1N1 were 4.4 times higher in subjects receiving more than five inactivated whole influenza virus vaccinations than those subjects with no record of vaccination. Although unclear if the result of prior vaccination or disease exposure, these pre-existing antibodies may prevent or reduce disease severity.

Author Notes

* Address correspondence to Sathit Pichyangkul, USAMC—Armed Forces Research Institute of Medical Sciences, Department of Immunology and Medicine, 315/6 Rajvithi Road, Phayathai, Bangkok 10400, Thailand. E-mail: SathitP@afrims.org

Financial support: This research was supported by National Institutes of Health, National Institute of Allergy and Infectious Diseases Grant Y1-AI-5026-01, the Thailand Research Fund, and Chulalongkorn University.

Authors' addresses: Sathit Pichyangkul, Somporn Krasaesub, Kosol Yongvanitchit, Amporn Limsalakpetch, Utaiwan Kum-Arb, Duangrat Mongkolsirichaikul, Nuanpan Khemnu, Carl J. Mason, Douglas S. Walsh, and David L. Saunders, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand, E-mails: SathitP@afrims.org, SompornK@afrims.org, KosolY@afrims.org, AmpornL@afrims.org, UtaiwanS@afrims.org, DuangratM@afrims.org, NuanpanK@afrims.org, Carl.Mason@afrims.org. douglas.s.walsh@us.army.mil, and David.Saunders@afrims.org. Anan Jongkaewwattana, National Center for Genetic Engineering and Biotechnology, Pathum Thani, Thailand, E-mail: anan.jon@biotec.or.th. Arunee Thitithanyanont and Suwimon Wiboon-ut, Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand, E-mails: scaty@mahidol.ac.th and swiboonut@gmail.com. Rangsini Mahanonda, Department of Dentistry, Chulalongkorn University, Bangkok, Thailand, E-mail: rangsini.m@chula.ac.th. Jean-Michel Garcia, Hong Kong University—Pasteur Research Center, Hong Kong, E-mail: jmgarcia@hku.hk.

Copyright:
© The American Society of Tropical Medicine and Hygiene 2014
Received:
22 Mar 2013
|
Accepted:
21 Jul 2013
|
Published Online:
08 Jan 2014
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Online ISSN:
1476-1645
  • 1.

    Rubertone MV, Brundage JF, 2002. The Defense Medical Surveillance System and the Department of Defense serum repository: glimpses of the future of public health surveillance. Am J Public Health 92: 1900–1904.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 2.

    Lee MS, Yang CF, 2003. Cross-reactive H1N1 antibody responses to a live attenuated influenza vaccine in children: implication for selection of vaccine strains. J Infect Dis 188: 1362–1366.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 3.

    Mendelman PM, Rappaport R, Cho I, Block S, Gruber W, August M, Dawson D, Cordova J, Kemble G, Mahmood K, Palladino G, Lee MS, Razmpour A, Stoddard J, Forrest BD, 2004. Live attenuated influenza vaccine induces cross-reactive antibody responses in children against an a/Fujian/411/2002-like H3N2 antigenic variant strain. Pediatr Infect Dis J 23: 1053–1055.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 4.

    Lambre CR, Terzidis H, Greffard A, Webster RG, 1990. Measurement of anti-influenza neuraminidase antibody using a peroxidase-linked lectin and microtitre plates coated with natural substrates. J Immunol Methods 135: 49–57.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 5.

    Garcia JM, Lagarde N, Ma ES, de Jong MD, Peiris JS, 2010. Optimization and evaluation of an influenza A (H5) pseudotyped lentiviral particle-based serological assay. J Clin Virol 47: 29–33.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 6.

    Koyama S, Aoshi T, Tanimoto T, Kumagai Y, Kobiyama K, Tougan T, Sakurai K, Coban C, Horii T, Akira S, Ishii KJ, 2010. Plasmacytoid dendritic cells delineate immunogenicity of influenza vaccine subtypes. Sci Transl Med 2: 25–24.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 7.

    Johns MC, Eick AA, Blazes DL, Lee SE, Perdue CL, Lipnick R, Vest KG, Russell KL, DeFraites RF, Sanchez JL, 2010. Seasonal influenza vaccine and protection against pandemic (H1N1) 2009-associated illness among US military personnel. PLoS One 5: e10722.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 8.

    Hancock K, Veguilla V, Lu X, Zhong W, Butler EN, Sun H, Liu F, Dong L, DeVos JR, Gargiullo PM, Brammer TL, Cox NJ, Tumpey TM, Katz JM, 2009. Cross-reactive antibody responses to the 2009 pandemic H1N1 influenza virus. N Engl J Med 361: 1945–1952.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 9.

    Tang JW, Tambyah PA, Wilder-Smith A, Puong KY, Shaw R, Barr IG, Chan KP, 2010. Crosss-reactive antibodies to pandemic (H1N1) 2009 virus, Singapore. Emerg Infect Dis 16: 874–876.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 10.

    Chen H, Wang Y, Liu W, Zhang J, Dong B, Fan X, de Jong MD, Farrar J, Riley S, Smith GJ, Guan Y, 2009. Serologic survey of pandemic (H1N1) 2009 virus, Guangxi Province, China. Emerg Infect Dis 15: 1849–1850.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 11.

    Grabenstein JD, Pittman PR, Greenwood JT, Engler RJ, 2006. Immunization to protect the US Armed Forces: heritage, current practice, and prospects. Epidemiol Rev 28: 3–26.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 12.

    Murphy BR, Kasel JA, Chanock RM, 1972. Association of serum anti-neuraminidase antibody with resistance to influenza in man. N Engl J Med 286: 1329–1332.

  • 13.

    Sandbulte MR, Jimenez GS, Boon AC, Smith LR, Treanor JJ, Webby RJ, 2007. Cross-reactive neuraminidase antibodies afford partial protection against H5N1 in mice and are present in unexposed humans. PLoS Med 4: e59.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 14.

    Smallman-Raynor M, Cliff AD, 2007. Avian influenza A (H5N1) age distribution in humans. Emerg Infect Dis 13: 510–512.

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