ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
World Health Organization, 2007. World Health Organization Global Strategy for the Prevention and Control of Sexually Transmitted Infections. Geneva: World Health Organization, 2006–2015.
-
2.
Simms I, Warburton F, Westrom L, 2003. Diagnosis of pelvic inflammatory disease: time for a rethink. Sex Transm Infect 79: 491–494.
-
3.
Gore-Felton C, Vosvick M, Bendel T, Koopman C, Das B, Israelski D, Herrera M, Litzenberg K, Spiegel D, 2003. Correlates of sexually transmitted disease infection among adults living with HIV. Int J STD AIDS 14: 539–546.
-
4.
Van Der Pol B, Kwok C, Pierre-Louis B, Rinaldi A, Salata RA, Chen PL, van de Wijgert J, Mmiro F, Mugerwa R, Chipato T, Morrison CS, 2008. Trichomonas vaginalis infection and human immunodeficiency virus acquisition in African women. J Infect Dis 197: 548–554.
-
5.
Sankuntaw N, Sukprasert S, Engchanil C, Kaewkes W, Chantratita W, Pairoj V, Lulitanond V, 2011. Single tube multiplex real-time PCR for the rapid detection of herpes virus infections of the central nervous system. Mol Cell Probes 25: 114–120.
-
6.
Muvunyi CM, Dhont N, Verhelst R, Crucitti T, Reijans M, Mulders B, Simons G, Temmerman M, Claeys G, Padalko E, 2011. Evaluation of a new multiplex polymerase chain reaction assay STD finder for the simultaneous detection of 7 sexually transmitted disease pathogens. Diagn Microbiol Infect Dis 71: 29–37.
-
7.
McIver CJ, Rismanto N, Smith C, Naing ZW, Rayner B, Lusk MJ, Konecny P, White PA, Rawlinson WD, 2009. Multiplex PCR testing detection of higher-than-expected rates of cervical mycoplasma, ureaplasma, and Trichomonas and viral agent infection in sexually Australian women. J Clin Microbiol 47: 1358–1363.
-
8.
McKechnie ML, Hillman R, Couldwell D, Kong F, Freedman E, Wang H, Gilbert GL, 2009. Simultaneous identification of 14 genital microorganisms in urine by use of a multiplex PCR-based reverse line blot assay. J Clin Microbiol 47: 1871–1877.
-
9.
Quint KD, de Koning MN, Geraets DT, Quint WG, Pirog EC, 2009. Comprehensive analysis of human papillomavirus and Chlamydia trachomatis in-situ and invasive cervical adenocarcinoma. Gynecol Oncol 114: 390–394.
-
10.
Teixeira PR, Vitória MA, Barcarolo J, 2004. Antiretroviral treatment in resource-poor settings: the Brazilian experience. AIDS 18 (Suppl 3): S5–S7.
-
11.
van de Wijgert JH, Morrison CS, Brown J, Kwok C, Van Der Pol B, Chipato T, Byamugisha JK, Padian N, Salata RA, 2009. Disentangling contributions of reproductive tract infections to HIV acquisition in African women. Sex Transm Dis 36: 357–364.
-
12.
Diaz N, Dessì D, Dessole S, Fiori PL, Rappelli P, 2010. Rapid detection of coinfections by Trichomonas vaginalis, Mycoplasma hominis and Ureaplasma urealyticum by a new multiplex polymerase chain reaction. Diagn Microbiol Infect Dis 67: 30–36.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 6 | 5 | 5 |
| Full Text Views | 448 | 163 | 3 |
| PDF Downloads | 182 | 67 | 3 |
Simultaneous Detection of Seven Sexually Transmitted Agents in Human Immunodeficiency Virus–Infected Brazilian Women by Multiplex Polymerase Chain Reaction
We determined the prevalence of seven clinically important pathogens that cause sexually transmitted infections (STIs) (Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, herpes simplex virus 1 [HSV-1], HSV-2, and Treponema pallidum), by using a multiplex polymerase chain reaction (M-PCR) in samples from Brazilian woman infected with human immunodeficiency virus 1 (HIV-1) and uninfected Brazilian women (controls). The M-PCR assay identified all STIs tested for and surprisingly, occurred association between the control and STIs. This association was probably caused by excellent HIV infection control and regular monitoring in these women established by public health strategies in Brazil to combat HIV/acquired immunodeficiency syndrome. Studies using this M-PCR in different populations may help to better elucidate the roles of STIs in several conditions.
Author Notes
Financial support: This work was supported by grants from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, AUX-PE-PRODOC 2571/2010, Brazilian Government.
Disclosure: None of the authors have any commercial or other conflicts of interest.
Authors' addresses: Raquel P. Souza, André L. P. de Abreu, Fabrícia Gimenes, and Marcia E. L. Consolaro, Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá, Av. Colombo, 5790, 87020900, Maringá, Paraná, Brazil, E-mails: raquelpantarotto@hotmail.com.br, andreluelsdorf@gmail.com, fabricia.gimenes@gmail.com, and melconsolaro@gmail.com. Érika C. Ferreira, Departamento de Estatística, Universidade Estadual de Maringá, Av. Colombo, 5790, 87020900, Maringá, Paraná, Brazil, E-mail: erikacris84@hotmail.com. Sheila C. Rocha-Brischiliari, Maria D. de B. Carvalho, and Sandra M. Pelloso, Departamento de Enfermagem, Universidade Estadual de Maringá, Av. Colombo, 5790, 87020900, Maringá, Paraná, Brazil, E-mails: sheilarocha.enfermeira@hotmail.com, mdbcarvalho@gmail.com, and smpelloso@gmail.com. Marcelo G. Bonini, College of Medicine, Department of Pharmacology, University of Illinois at Chicago, Chicago, IL, E-mail: mbonini@uic.edu.