Feasey N, Jones MW, Mabey DC, Solomon AW, 2010. Neglected tropical diseases. Br Med Bull 93: 179–200.
Murray HW, Berman JD, Davies CR, Saravia NG, 2005. Advances in leishmaniasis. Lancet 366: 1561–1577.
Roberts MT, 2006. Current understandings on the immunology of leishmaniasis and recent developments in prevention and treatment. Br Med Bull 75–76: 115–130. doi:10.1093/bmb/ldl003.
Berman JD, 1997. Human leishmaniasis: clinical, diagnostic and therapeutic developments in the last 10 years. Clin Infect Dis 24: 684–703.
Andresen K, Gaafar A, El-Hasan AM, Ismail A, Dafaila M, Theander TG, Kharazmi A, 1996. Evaluation of the polymerase chain reaction in the diagnosis of cutaneous leishmaniasis due to Leishmania major. A comparison with direct microscopy of smears and sections from lesions. Trans R Soc Trop Med Hyg 90: 133–135.
Aviles H, Belli A, Armijos R, Monroy F, Harris E, 1999. PCR detection and identification of Leishmania parasites in clinical specimens in Ecuador: a comparison with classical diagnostic methods. J Parasitol 85: 181–187.
Mary C, Faraut FO, Lascombe L, Dumon H, 2004. Quantification of Leishmania infantum DNA by a real-time PCR assay with high sensitivity. J Clin Microbiol 42: 5249–5255.
Shahbazi F, Shahabi S, Kazemi B, Mohebali M, Abadi AR, Zare Z, 2008. Evaluation of PCR assay in diagnosis and identification of cutaneous leishmaniasis: a comparison with the parasitological methods. Parasitol Res 103: 1159–1162.
Osman OF, Oskam L, Zijlstra EE, Kroon NC, Schoone GJ, Khalil ET, EL-Hassan AM, Kager PA, 1997. Evaluation of PCR for diagnosis of visceral leishmaniasis. J Clin Microbiol 35: 2454–2457.
Lightner LK, Chulay JD, Bryceson AD, 1983. Comparison of microscopy and culture in the detection of Leishmania donovani from splenic aspirates. Am J Trop Med Hyg 32: 296–299.
Sukmee T, Siripattanapipong S, Mungthin M, Worapong J, Rangsin R, Samung Y, Kongkaew W, Bumrungsana K, Chanachai K, Apiwathanasorn C, Rujirojindakul P, Wattanasri S, Ungchusak K, Leelayoova S, 2008. A suspected new species of Leishmania, the causative agent of visceral leishmaniasis in a Thai patient. Int J Parasitol 38: 617–622.
Suankratay C, Suwanpimolkul G, Wilde H, Siriyasatien P, 2010. Case report: autochthonous visceral leishmaniasis in a human immunodeficiency virus (HIV)-infected patient: the first in Thailand and review of the literature. Am J Trop Med Hyg 82: 4–8.
Kongkaew W, Siriarayaporn P, Leelayoova S, Supparatpinyo K, Areechokchai D, Duang-ngern P, Chanachai K, Sukmee T, Samung Y, Sridurongkathum P, 2007. Autochthonous visceral leishmaniasis: a report of a second case in Thailand. Southeast Asian J Trop Med Public Health 38: 8–12.
Chusri S, Hortiwakul T, Silpapojakul K, Siriyasatien P, 2012. Case report: consecutive cutaneous and visceral leishmaniasis manifestations involving a novel Leishmania species in two HIV patients in Thailand. Am J Trop Med Hyg 87: 76–80.
Bualert L, Charungkiattikul W, Thongsuksai P, Mungthin M, Siripattanapipong S, Khositnithikul R, Naaglor T, Ravel C, Baidouri FE, Leelayoova S, 2012. Case report: autochthonous disseminated dermal and visceral leishmaniasis in an AIDS patient, southern Thailand, caused by Leishmania siamensis. Am J Trop Med Hyg 86: 821–824.
Viriyavejakul P, Viravan C, Riganti M, Punpoowong B, 1997. Imported cutaneous leishmaniasis in Thailand. Southeast Asian J Trop Med Public Health 28: 558–562.
Maharom P, Siripattanapipong S, Mungthin M, Naaglor T, Sukkawee R, Pudkorn R, Wattana W, Wanachiwanawin D, Areechokchai D, Leelayoova S, 2008. Visceral leishmaniasis caused by Leishmania infantum in Thailand. Southeast Asian J Trop Med Public Health 39: 988–990.
Corvalan FH, Sampaio RN, Brustoloni YM, Andreotti R, Lima Júnior MS, 2011. DNA identification of Leishmania (Viannia) braziliensis in human saliva from a patient with American cutaneous leishmaniasis. J Venom Anim Toxins Incl Trop Dis 17: 98–102.
Galaï Y, Chabchoub N, Abid MB, Abda IB, Bouafif NB, Amri F, Aoun K, Bouratbine A, 2011. Diagnosis of Mediterranean visceral leishmaniasis by detection of Leishmania antibodies and Leishmania DNA in oral fluid samples collected using an Oracol device. J Clin Microbiol 49: 3150–3153.
Forkner CE, Zia LS, 1934. Viable Leishmania donovani in nasal and oral secretions of patients with kala-azar and the bearing of this finding on the transmission of the disease. J Exp Med 59: 491–499.
Spanakos G, Piperaki ET, Menounos PG, Tegos N, Flemetakis A, Vakalis NC, 2007. Detection and species identification of Old World Leishmania in clinical samples using a PCR-based method. Trans R Soc Trop Med Hyg 102: 46–53.
Van Eys GJ, Schoone GJ, Kroon NC, Ebeling SB, 1992. Sequence analysis of small subunit ribosomal RNA genes and its use for detection and identification of Leishmania parasites. Mol Biochem Parasitol 51: 133–142.
Kwok S, Higuchi R, 1989. Avoiding false positives with PCR. Nature 339: 237–238.
Mebrahtu YB, Hendricks LD, Oster CN, Lawyer PG, Perkins PV, Pamba H, 1993. Leishmania donovani parasites in the nasal secretions, tonsillopharyngeal mucosa, and urine centrifugates of visceral leishmaniasis patients in Kenya. Am J Trop Med Hyg 48: 530–535.
Efstratiadis G, Boura E, Giamalis P, Mandala E, Leontsini M, Tsiaousis G, Memmos D, 2006. Renal involvement in a patient with visceral leishmaniasis. Nephrol Dial Transplant 21: 235–236.
Dutra M, Martinelli R, de Carvalho EM, Rodrigues LE, Brito E, Rocha H, 1985. Renal involvement in visceral leishmaniasis. Am J Kidney Dis 6: 22–27.
Amann K, Bogdan C, Harrer T, Rech J, 2012. Renal leishmaniasis as unusual cause of nephrotic syndrome in an HIV patient. J Am Soc Nephrol 23: 586–590.
Romero GA, Guerra MV, Paes MG, Cupolillo E, Bentin Toaldo C, Macedo VO, Fernandes O, 2001. Sensitivity of the polymerase chain reaction for the diagnosis of cutaneous leishmaniasis due to Leishmania (Viannia) guyanensis. Acta Trop 79: 225–229.
Mathis A, Deplazes P, 1995. PCR and in vitro cultivation for detection of Leishmania spp. in diagnostic samples from humans and dogs. J Clin Microbiol 33: 1145–1149.
Smits HL, Hartskeerl RA, 1995. PCR amplification reactions in parasitology. J Microbiol Methods 23: 41–54.
Piarroux R, Azaiez R, Lossi AM, Reynier P, Muscatelli F, Gambarelli F, Fontes M, Dumon H, Quilici M, 1993. Isolation and characterization of a repetitive DNA sequence from Leishmania infantum: development of a visceral leishmaniasis polymerase chain reaction. Am J Trop Med Hyg 49: 364–369.
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Polymerase chain reaction was used to detect Leishmania siamensis DNA from clinical samples collected from six leishmaniasis patients during 2011–2012. The samples used in this study came from bone marrow, blood, buffy coat, saliva, urine, and tissue biopsy specimens. Saliva was a good source for L. siamensis DNA by polymerase chain reaction. L. siamensis DNA was also found in saliva of an asymptomatic case-patient. Levels of L. siamensis DNA in saliva decreased until being undetectable after treatment. These levels could be used as a marker to evaluate efficacy of the treatment. A larger study is needed to evaluate this method as a screening and survey tool to study the silent background of Leishmania infection among the at-risk population.
Financial support: This study was supported by Integrated Innovation Academic Center: IIAC Chulalongkorn University Centenary Academic Development Project; the Higher Education Research Promotion and National Research University Project of Thailand; the Office of the Higher Education Commission (HR1160A-56), a tuition fee scholarship from the Graduate School, Chulalongkorn University; the Thailand Research Fund to Wej Choochote (TRF Senior Research Scholar: RTA5480006); the Ratchadapisak Sompotch Fund, Faculty of Medicine, Chulalongkorn University (grant no. RA (MF) 02/56); and the National Science and Technology Development Agency (Thailand).
Authors' addresses: Atchara Phumee, Medical Science Programme, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand, E-mail: amphumee@gmail.com. Kanyarat Kraivichian and Vivornpun Sanprasert, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand, E-mails: iamteaw@yahoo.com and vivornpun@gmail.com. Sarunyou Chusri, Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, Thailand, E-mail: sarunyouchusri@hotmail.com. Nopadon Noppakun, Division of Dermatology, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand and Bumrungrad International Hospital, Wattana, Bangkok, 10110 Thailand, E-mail: dr.nopadon@yahoo.com. Asda Vibhagool, Bumrungrad International Hospital, Wattana, Bangkok 10110, Thailand, E-mail: asdavi@gmail.com. Vich Tampanya, Chiangrai Prachanukroh Hospital, Chiang Rai, Chiang Rai 57000, Thailand, E-mail: aekcha@gmail.com. Henry Wilde, Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok 10330 Thailand, E-mail: wildehenry@yahoo.com. Padet Siriyasatien, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand, and Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand, E-mail: padet.s@chula.ac.th.
Feasey N, Jones MW, Mabey DC, Solomon AW, 2010. Neglected tropical diseases. Br Med Bull 93: 179–200.
Murray HW, Berman JD, Davies CR, Saravia NG, 2005. Advances in leishmaniasis. Lancet 366: 1561–1577.
Roberts MT, 2006. Current understandings on the immunology of leishmaniasis and recent developments in prevention and treatment. Br Med Bull 75–76: 115–130. doi:10.1093/bmb/ldl003.
Berman JD, 1997. Human leishmaniasis: clinical, diagnostic and therapeutic developments in the last 10 years. Clin Infect Dis 24: 684–703.
Andresen K, Gaafar A, El-Hasan AM, Ismail A, Dafaila M, Theander TG, Kharazmi A, 1996. Evaluation of the polymerase chain reaction in the diagnosis of cutaneous leishmaniasis due to Leishmania major. A comparison with direct microscopy of smears and sections from lesions. Trans R Soc Trop Med Hyg 90: 133–135.
Aviles H, Belli A, Armijos R, Monroy F, Harris E, 1999. PCR detection and identification of Leishmania parasites in clinical specimens in Ecuador: a comparison with classical diagnostic methods. J Parasitol 85: 181–187.
Mary C, Faraut FO, Lascombe L, Dumon H, 2004. Quantification of Leishmania infantum DNA by a real-time PCR assay with high sensitivity. J Clin Microbiol 42: 5249–5255.
Shahbazi F, Shahabi S, Kazemi B, Mohebali M, Abadi AR, Zare Z, 2008. Evaluation of PCR assay in diagnosis and identification of cutaneous leishmaniasis: a comparison with the parasitological methods. Parasitol Res 103: 1159–1162.
Osman OF, Oskam L, Zijlstra EE, Kroon NC, Schoone GJ, Khalil ET, EL-Hassan AM, Kager PA, 1997. Evaluation of PCR for diagnosis of visceral leishmaniasis. J Clin Microbiol 35: 2454–2457.
Lightner LK, Chulay JD, Bryceson AD, 1983. Comparison of microscopy and culture in the detection of Leishmania donovani from splenic aspirates. Am J Trop Med Hyg 32: 296–299.
Sukmee T, Siripattanapipong S, Mungthin M, Worapong J, Rangsin R, Samung Y, Kongkaew W, Bumrungsana K, Chanachai K, Apiwathanasorn C, Rujirojindakul P, Wattanasri S, Ungchusak K, Leelayoova S, 2008. A suspected new species of Leishmania, the causative agent of visceral leishmaniasis in a Thai patient. Int J Parasitol 38: 617–622.
Suankratay C, Suwanpimolkul G, Wilde H, Siriyasatien P, 2010. Case report: autochthonous visceral leishmaniasis in a human immunodeficiency virus (HIV)-infected patient: the first in Thailand and review of the literature. Am J Trop Med Hyg 82: 4–8.
Kongkaew W, Siriarayaporn P, Leelayoova S, Supparatpinyo K, Areechokchai D, Duang-ngern P, Chanachai K, Sukmee T, Samung Y, Sridurongkathum P, 2007. Autochthonous visceral leishmaniasis: a report of a second case in Thailand. Southeast Asian J Trop Med Public Health 38: 8–12.
Chusri S, Hortiwakul T, Silpapojakul K, Siriyasatien P, 2012. Case report: consecutive cutaneous and visceral leishmaniasis manifestations involving a novel Leishmania species in two HIV patients in Thailand. Am J Trop Med Hyg 87: 76–80.
Bualert L, Charungkiattikul W, Thongsuksai P, Mungthin M, Siripattanapipong S, Khositnithikul R, Naaglor T, Ravel C, Baidouri FE, Leelayoova S, 2012. Case report: autochthonous disseminated dermal and visceral leishmaniasis in an AIDS patient, southern Thailand, caused by Leishmania siamensis. Am J Trop Med Hyg 86: 821–824.
Viriyavejakul P, Viravan C, Riganti M, Punpoowong B, 1997. Imported cutaneous leishmaniasis in Thailand. Southeast Asian J Trop Med Public Health 28: 558–562.
Maharom P, Siripattanapipong S, Mungthin M, Naaglor T, Sukkawee R, Pudkorn R, Wattana W, Wanachiwanawin D, Areechokchai D, Leelayoova S, 2008. Visceral leishmaniasis caused by Leishmania infantum in Thailand. Southeast Asian J Trop Med Public Health 39: 988–990.
Corvalan FH, Sampaio RN, Brustoloni YM, Andreotti R, Lima Júnior MS, 2011. DNA identification of Leishmania (Viannia) braziliensis in human saliva from a patient with American cutaneous leishmaniasis. J Venom Anim Toxins Incl Trop Dis 17: 98–102.
Galaï Y, Chabchoub N, Abid MB, Abda IB, Bouafif NB, Amri F, Aoun K, Bouratbine A, 2011. Diagnosis of Mediterranean visceral leishmaniasis by detection of Leishmania antibodies and Leishmania DNA in oral fluid samples collected using an Oracol device. J Clin Microbiol 49: 3150–3153.
Forkner CE, Zia LS, 1934. Viable Leishmania donovani in nasal and oral secretions of patients with kala-azar and the bearing of this finding on the transmission of the disease. J Exp Med 59: 491–499.
Spanakos G, Piperaki ET, Menounos PG, Tegos N, Flemetakis A, Vakalis NC, 2007. Detection and species identification of Old World Leishmania in clinical samples using a PCR-based method. Trans R Soc Trop Med Hyg 102: 46–53.
Van Eys GJ, Schoone GJ, Kroon NC, Ebeling SB, 1992. Sequence analysis of small subunit ribosomal RNA genes and its use for detection and identification of Leishmania parasites. Mol Biochem Parasitol 51: 133–142.
Kwok S, Higuchi R, 1989. Avoiding false positives with PCR. Nature 339: 237–238.
Mebrahtu YB, Hendricks LD, Oster CN, Lawyer PG, Perkins PV, Pamba H, 1993. Leishmania donovani parasites in the nasal secretions, tonsillopharyngeal mucosa, and urine centrifugates of visceral leishmaniasis patients in Kenya. Am J Trop Med Hyg 48: 530–535.
Efstratiadis G, Boura E, Giamalis P, Mandala E, Leontsini M, Tsiaousis G, Memmos D, 2006. Renal involvement in a patient with visceral leishmaniasis. Nephrol Dial Transplant 21: 235–236.
Dutra M, Martinelli R, de Carvalho EM, Rodrigues LE, Brito E, Rocha H, 1985. Renal involvement in visceral leishmaniasis. Am J Kidney Dis 6: 22–27.
Amann K, Bogdan C, Harrer T, Rech J, 2012. Renal leishmaniasis as unusual cause of nephrotic syndrome in an HIV patient. J Am Soc Nephrol 23: 586–590.
Romero GA, Guerra MV, Paes MG, Cupolillo E, Bentin Toaldo C, Macedo VO, Fernandes O, 2001. Sensitivity of the polymerase chain reaction for the diagnosis of cutaneous leishmaniasis due to Leishmania (Viannia) guyanensis. Acta Trop 79: 225–229.
Mathis A, Deplazes P, 1995. PCR and in vitro cultivation for detection of Leishmania spp. in diagnostic samples from humans and dogs. J Clin Microbiol 33: 1145–1149.
Smits HL, Hartskeerl RA, 1995. PCR amplification reactions in parasitology. J Microbiol Methods 23: 41–54.
Piarroux R, Azaiez R, Lossi AM, Reynier P, Muscatelli F, Gambarelli F, Fontes M, Dumon H, Quilici M, 1993. Isolation and characterization of a repetitive DNA sequence from Leishmania infantum: development of a visceral leishmaniasis polymerase chain reaction. Am J Trop Med Hyg 49: 364–369.
Past two years | Past Year | Past 30 Days | |
---|---|---|---|
Abstract Views | 22 | 22 | 6 |
Full Text Views | 436 | 170 | 0 |
PDF Downloads | 90 | 24 | 0 |