Detection of Leishmania siamensis DNA in Saliva by Polymerase Chain Reaction

Atchara Phumee Medical Sciences Program, Department of Parasitology, Division of Dermatology, and Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand; Bumrungrad International Hospital, Bangkok, Thailand; Chiangrai Prachanukroh Hospital, Chiang Rai, Thailand; Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand

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Kanyarat Kraivichian Medical Sciences Program, Department of Parasitology, Division of Dermatology, and Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand; Bumrungrad International Hospital, Bangkok, Thailand; Chiangrai Prachanukroh Hospital, Chiang Rai, Thailand; Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand

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Sarunyou Chusri Medical Sciences Program, Department of Parasitology, Division of Dermatology, and Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand; Bumrungrad International Hospital, Bangkok, Thailand; Chiangrai Prachanukroh Hospital, Chiang Rai, Thailand; Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand

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Nopadon Noppakun Medical Sciences Program, Department of Parasitology, Division of Dermatology, and Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand; Bumrungrad International Hospital, Bangkok, Thailand; Chiangrai Prachanukroh Hospital, Chiang Rai, Thailand; Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand

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Asda Vibhagool Medical Sciences Program, Department of Parasitology, Division of Dermatology, and Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand; Bumrungrad International Hospital, Bangkok, Thailand; Chiangrai Prachanukroh Hospital, Chiang Rai, Thailand; Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand

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Vivornpun Sanprasert Medical Sciences Program, Department of Parasitology, Division of Dermatology, and Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand; Bumrungrad International Hospital, Bangkok, Thailand; Chiangrai Prachanukroh Hospital, Chiang Rai, Thailand; Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand

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Vich Tampanya Medical Sciences Program, Department of Parasitology, Division of Dermatology, and Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand; Bumrungrad International Hospital, Bangkok, Thailand; Chiangrai Prachanukroh Hospital, Chiang Rai, Thailand; Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand

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Henry Wilde Medical Sciences Program, Department of Parasitology, Division of Dermatology, and Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand; Bumrungrad International Hospital, Bangkok, Thailand; Chiangrai Prachanukroh Hospital, Chiang Rai, Thailand; Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand

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Padet Siriyasatien Medical Sciences Program, Department of Parasitology, Division of Dermatology, and Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand; Bumrungrad International Hospital, Bangkok, Thailand; Chiangrai Prachanukroh Hospital, Chiang Rai, Thailand; Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand

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Polymerase chain reaction was used to detect Leishmania siamensis DNA from clinical samples collected from six leishmaniasis patients during 2011–2012. The samples used in this study came from bone marrow, blood, buffy coat, saliva, urine, and tissue biopsy specimens. Saliva was a good source for L. siamensis DNA by polymerase chain reaction. L. siamensis DNA was also found in saliva of an asymptomatic case-patient. Levels of L. siamensis DNA in saliva decreased until being undetectable after treatment. These levels could be used as a marker to evaluate efficacy of the treatment. A larger study is needed to evaluate this method as a screening and survey tool to study the silent background of Leishmania infection among the at-risk population.

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Author Notes

* Address correspondence to Padet Siriyasatien, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand and Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand. E-mail: padet.s@chula.ac.th

Financial support: This study was supported by Integrated Innovation Academic Center: IIAC Chulalongkorn University Centenary Academic Development Project; the Higher Education Research Promotion and National Research University Project of Thailand; the Office of the Higher Education Commission (HR1160A-56), a tuition fee scholarship from the Graduate School, Chulalongkorn University; the Thailand Research Fund to Wej Choochote (TRF Senior Research Scholar: RTA5480006); the Ratchadapisak Sompotch Fund, Faculty of Medicine, Chulalongkorn University (grant no. RA (MF) 02/56); and the National Science and Technology Development Agency (Thailand).

Authors' addresses: Atchara Phumee, Medical Science Programme, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand, E-mail: amphumee@gmail.com. Kanyarat Kraivichian and Vivornpun Sanprasert, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand, E-mails: iamteaw@yahoo.com and vivornpun@gmail.com. Sarunyou Chusri, Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, Thailand, E-mail: sarunyouchusri@hotmail.com. Nopadon Noppakun, Division of Dermatology, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand and Bumrungrad International Hospital, Wattana, Bangkok, 10110 Thailand, E-mail: dr.nopadon@yahoo.com. Asda Vibhagool, Bumrungrad International Hospital, Wattana, Bangkok 10110, Thailand, E-mail: asdavi@gmail.com. Vich Tampanya, Chiangrai Prachanukroh Hospital, Chiang Rai, Chiang Rai 57000, Thailand, E-mail: aekcha@gmail.com. Henry Wilde, Division of Infectious Diseases, Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok 10330 Thailand, E-mail: wildehenry@yahoo.com. Padet Siriyasatien, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand, and Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand, E-mail: padet.s@chula.ac.th.

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