ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Repetto SA, Duran PA, Lasala MB, Gonzalez-Cappa SM, 2010. High rate of strongyloidosis infection, out of endemic area, in patients with eosinophilia and without risk of exogenous reinfections. Am J Trop Med Hyg 82: 1088–1093.
-
2.
Winsberg GR, Sonnenschein E, Dyer AR, Schnadig V, Bonilla E, 1975. Prevalence of intestinal parasites in Latino residents of Chicago. Am J Epidemiol 102: 526–532.
-
3.
Sato Y, Kobayashi J, Toma H, Shiroma Y, 1995. Efficacy of stool examination for detection of Strongyloides infection. Am J Trop Med Hyg 53: 248–250.
-
4.
Hirata T, Nakamura H, Kinjo N, Hokama A, Kinjo F, Yamane N, Fujita J, 2007. Increased detection rate of Strongyloides stercoralis by repeated stool examinations using the agar plate culture method. Am J Trop Med Hyg 77: 683–684.
-
5.
Ganesh S, Cruz RJ Jr, 2011. Strongyloidiasis: a multifaceted disease. Gastroenterol Hepatol 7: 194–196.
-
6.
van Doorn HR, Koelewijn R, Hofwegen H, Gilis H, Wetsteyn JC, Wismans PJ, Sarfati C, Vervoort T, van Gool T, 2007. Use of enzyme-linked immunosorbent assay and dipstick assay for detection of Strongyloides stercoralis infection in humans. J Clin Microbiol 45: 438–442.
-
7.
Rodrigues RM, de Oliveira MC, Sopelete MC, Silva DA, Campos DM, Taketomi EA, Costa-Cruz JM, 2007. IgG1, IgG4, and IgE antibody responses in human strongyloidiasis by ELISA using Strongyloides ratti saline extract as heterologous antigen. Parasitol Res 101: 1209–1214.
-
8.
Olsen A, van Lieshout L, Marti H, Polderman T, Polman K, Steinmann P, Stothard R, Thybo S, Verweij JJ, Magnussen P, 2009. Strongyloidiasis: the most neglected of the neglected tropical diseases? Trans R Soc Trop Med Hyg 103: 967–972.
-
9.
Krolewiecki AJ, Ramanathan R, Fink V, McAuliffe I, Cajal SP, Won K, Juarez M, Di Paolo A, Tapia L, Acosta N, Lee R, Lammie P, Abraham D, Nutman TB, 2010. Improved diagnosis of Strongyloides stercoralis using recombinant antigen-based serologies in a community-wide study in northern Argentina. Clin Vaccine Immunol 17: 1624–1630.
-
10.
Verweij JJ, Canales M, Polman K, Ziem J, Brienen EA, Polderman AM, van Lieshout L, 2009. Molecular diagnosis of Strongyloides stercoralis in faecal samples using real-time PCR. Trans R Soc Trop Med Hyg 103: 342–346.
-
11.
Kramme S, Nissen N, Soblik H, Erttmann K, Tannich E, Fleischer B, Panning M, Brattig N, 2011. Novel real-time PCR for the universal detection of Strongyloides species. J Med Microbiol 60: 454–458.
-
12.
Basuni M, Muhi J, Othman N, Verweij JJ, Ahmad M, Miswan N, Rahumatullah A, Aziz FA, Zainudin NS, Noordin R, 2011. A pentaplex real-time polymerase chain reaction assay for detection of four species of soil-transmitted helminths. Am J Trop Med Hyg 84: 338–343.
-
13.
Wilde J, Eiden J, Yolken R, 1990. Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions. J Clin Microbiol 28: 1300–1307.
-
14.
Valentine-Thon E, 2002. Quality control in nucleic acid testing: where do we stand? J Clin Virol 25 (Suppl 3): S13–S21.
-
15.
Boom R, Sol C, Weel J, Lettinga K, Gerrits Y, van Breda A, Wertheim-Van Dillen P, 2000. Detection and quantitation of human cytomegalovirus DNA in faeces. J Virol Methods 84: 1–14.
-
16.
Beld M, Minnaar R, Weel J, Sol C, Damen M, van der Avoort H, Wertheim-van Dillen P, van Breda A, Boom R, 2004. Highly sensitive assay for detection of enterovirus in clinical specimens by reverse transcription-PCR with an armored RNA internal control. J Clin Microbiol 42: 3059–3064.
-
17.
Fredricks DN, Smith C, Meier A, 2005. Comparison of six DNA extraction methods for recovery of fungal DNA as assessed by quantitative PCR. J Clin Microbiol 43: 5122–5128.
-
18.
Garcia LS, 2001. Diagnostic Medical Parasitology. Fourth edition. Washington, DC: American Society for Microbiology Press.
-
19.
Yu Z, Morrison M, 2004. Improved extraction of PCR-quality community DNA from digesta and fecal samples. Biotechniques 36: 808–812.
-
20.
Nylund L, Heilig HG, Salminen S, de Vos WM, Satokari R, 2010. Semi-automated extraction of microbial DNA from stool for qPCR and phylogenetic microarray analysis. J Microbiol Methods 83: 231–235.
-
21.
Marra NM, Chiuso-Minicucci F, Machado GC, Zorzella-Pezavento SF, Franca TG, Ishikawa LL, Amarante AF, Sartori A, Amarante MR, 2010. Faecal examination and PCR to detect Strongyloides venezuelensis in experimentally infected Lewis rats. Mem Inst Oswaldo Cruz 105: 57–61.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 678 | 397 | 15 |
| Full Text Views | 510 | 12 | 0 |
| PDF Downloads | 190 | 17 | 0 |
Real-Time Polymerase Chain Reaction for Detection of Strongyloides stercoralis in Stool
Yasmin Sultana
Yasmin Sultana
Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, New South Wales, Australia; Discipline of Medicine, Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia
Search for other papers by Yasmin Sultana in
Current site
Google Scholar
PubMed
Search for other papers by Yasmin Sultana in
Current site
Google Scholar
PubMed
Neisha Jeoffreys
Neisha Jeoffreys
Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, New South Wales, Australia; Discipline of Medicine, Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia
Search for other papers by Neisha Jeoffreys in
Current site
Google Scholar
PubMed
Search for other papers by Neisha Jeoffreys in
Current site
Google Scholar
PubMed
Matthew R. Watts
Matthew R. Watts
Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, New South Wales, Australia; Discipline of Medicine, Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia
Search for other papers by Matthew R. Watts in
Current site
Google Scholar
PubMed
Search for other papers by Matthew R. Watts in
Current site
Google Scholar
PubMed
Gwendolyn L. Gilbert
Gwendolyn L. Gilbert
Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, New South Wales, Australia; Discipline of Medicine, Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia
Search for other papers by Gwendolyn L. Gilbert in
Current site
Google Scholar
PubMed
Search for other papers by Gwendolyn L. Gilbert in
Current site
Google Scholar
PubMed
Rogan Lee
Rogan Lee
Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, New South Wales, Australia; Discipline of Medicine, Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia
Search for other papers by Rogan Lee in
Current site
Google Scholar
PubMed
Search for other papers by Rogan Lee in
Current site
Google Scholar
PubMed
The use of real-time polymerase chain reaction (PCR) for detection of Strongyloides stercoralis in stool has recently been described. We compared five DNA extraction methods by using normal human stool spiked with Strongyloides ratti and tested by using a real-time PCR. The PowerSoil kit was found to be the best technique in terms of sensitivity and ease of use. The PCR detected DNA extracted from one spiked S. ratti larva diluted 10−2. The PowerSoil kit was then used to extract DNA from 160 human survey samples. All culture positive specimens with a high and moderate larval load were identified by real-time PCR, but only 15% of specimens with low larval load were positive. Specificity was greater than 99%. The combination of the PowerSoil kit and real-time PCR reliably detected high to moderate larval numbers of S. stercoralis in stools but was less sensitive when the larval load was low.
Author Notes
Financial support: This study was supported by an Australian Leadership Scholarship Award funded by The Australian Agency for International Development and in part by the Centre for Infectious Diseases and Microbiology–Public Health.
Authors' addresses: Yasmin Sultana, Neisha Jeoffreys, Matthew R. Watts, Gwendolyn L. Gilbert, and Rogan Lee, Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Darcy Road, Westmead, New South Wales 2145, Australia, E-mails: yasmin.du@yahoo.com, ysul7258@uni.sydney.edu.au, neisha.jeoffreys@swahs.health.nsw.gov.au, watts.idmicro@gmail.com, lyn.gilbert@sydney.edu.au, and rogan.lee@swahs.health.nsw.gov.au.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Repetto SA, Duran PA, Lasala MB, Gonzalez-Cappa SM, 2010. High rate of strongyloidosis infection, out of endemic area, in patients with eosinophilia and without risk of exogenous reinfections. Am J Trop Med Hyg 82: 1088–1093.
-
2.
Winsberg GR, Sonnenschein E, Dyer AR, Schnadig V, Bonilla E, 1975. Prevalence of intestinal parasites in Latino residents of Chicago. Am J Epidemiol 102: 526–532.
-
3.
Sato Y, Kobayashi J, Toma H, Shiroma Y, 1995. Efficacy of stool examination for detection of Strongyloides infection. Am J Trop Med Hyg 53: 248–250.
-
4.
Hirata T, Nakamura H, Kinjo N, Hokama A, Kinjo F, Yamane N, Fujita J, 2007. Increased detection rate of Strongyloides stercoralis by repeated stool examinations using the agar plate culture method. Am J Trop Med Hyg 77: 683–684.
-
5.
Ganesh S, Cruz RJ Jr, 2011. Strongyloidiasis: a multifaceted disease. Gastroenterol Hepatol 7: 194–196.
-
6.
van Doorn HR, Koelewijn R, Hofwegen H, Gilis H, Wetsteyn JC, Wismans PJ, Sarfati C, Vervoort T, van Gool T, 2007. Use of enzyme-linked immunosorbent assay and dipstick assay for detection of Strongyloides stercoralis infection in humans. J Clin Microbiol 45: 438–442.
-
7.
Rodrigues RM, de Oliveira MC, Sopelete MC, Silva DA, Campos DM, Taketomi EA, Costa-Cruz JM, 2007. IgG1, IgG4, and IgE antibody responses in human strongyloidiasis by ELISA using Strongyloides ratti saline extract as heterologous antigen. Parasitol Res 101: 1209–1214.
-
8.
Olsen A, van Lieshout L, Marti H, Polderman T, Polman K, Steinmann P, Stothard R, Thybo S, Verweij JJ, Magnussen P, 2009. Strongyloidiasis: the most neglected of the neglected tropical diseases? Trans R Soc Trop Med Hyg 103: 967–972.
-
9.
Krolewiecki AJ, Ramanathan R, Fink V, McAuliffe I, Cajal SP, Won K, Juarez M, Di Paolo A, Tapia L, Acosta N, Lee R, Lammie P, Abraham D, Nutman TB, 2010. Improved diagnosis of Strongyloides stercoralis using recombinant antigen-based serologies in a community-wide study in northern Argentina. Clin Vaccine Immunol 17: 1624–1630.
-
10.
Verweij JJ, Canales M, Polman K, Ziem J, Brienen EA, Polderman AM, van Lieshout L, 2009. Molecular diagnosis of Strongyloides stercoralis in faecal samples using real-time PCR. Trans R Soc Trop Med Hyg 103: 342–346.
-
11.
Kramme S, Nissen N, Soblik H, Erttmann K, Tannich E, Fleischer B, Panning M, Brattig N, 2011. Novel real-time PCR for the universal detection of Strongyloides species. J Med Microbiol 60: 454–458.
-
12.
Basuni M, Muhi J, Othman N, Verweij JJ, Ahmad M, Miswan N, Rahumatullah A, Aziz FA, Zainudin NS, Noordin R, 2011. A pentaplex real-time polymerase chain reaction assay for detection of four species of soil-transmitted helminths. Am J Trop Med Hyg 84: 338–343.
-
13.
Wilde J, Eiden J, Yolken R, 1990. Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions. J Clin Microbiol 28: 1300–1307.
-
14.
Valentine-Thon E, 2002. Quality control in nucleic acid testing: where do we stand? J Clin Virol 25 (Suppl 3): S13–S21.
-
15.
Boom R, Sol C, Weel J, Lettinga K, Gerrits Y, van Breda A, Wertheim-Van Dillen P, 2000. Detection and quantitation of human cytomegalovirus DNA in faeces. J Virol Methods 84: 1–14.
-
16.
Beld M, Minnaar R, Weel J, Sol C, Damen M, van der Avoort H, Wertheim-van Dillen P, van Breda A, Boom R, 2004. Highly sensitive assay for detection of enterovirus in clinical specimens by reverse transcription-PCR with an armored RNA internal control. J Clin Microbiol 42: 3059–3064.
-
17.
Fredricks DN, Smith C, Meier A, 2005. Comparison of six DNA extraction methods for recovery of fungal DNA as assessed by quantitative PCR. J Clin Microbiol 43: 5122–5128.
-
18.
Garcia LS, 2001. Diagnostic Medical Parasitology. Fourth edition. Washington, DC: American Society for Microbiology Press.
-
19.
Yu Z, Morrison M, 2004. Improved extraction of PCR-quality community DNA from digesta and fecal samples. Biotechniques 36: 808–812.
-
20.
Nylund L, Heilig HG, Salminen S, de Vos WM, Satokari R, 2010. Semi-automated extraction of microbial DNA from stool for qPCR and phylogenetic microarray analysis. J Microbiol Methods 83: 231–235.
-
21.
Marra NM, Chiuso-Minicucci F, Machado GC, Zorzella-Pezavento SF, Franca TG, Ishikawa LL, Amarante AF, Sartori A, Amarante MR, 2010. Faecal examination and PCR to detect Strongyloides venezuelensis in experimentally infected Lewis rats. Mem Inst Oswaldo Cruz 105: 57–61.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 678 | 397 | 15 |
| Full Text Views | 510 | 12 | 0 |
| PDF Downloads | 190 | 17 | 0 |