- Introduction
- Material And Methods
- Results
- Identification of diagnostic proteins.
- Specificity of the P. kellicotti diagnostic proteins.
- Reactivity to excretory/secretory P. kellicotti antigens.
- Emergence and persistence of antibodies to P. kellicotti antigens.
- Comparison of Western blot results obtained with P. kellicotti and P. westermani adult worm antigens.
- Discussion
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Centers for Disease Control and Prevention, 2010. Human paragonimiasis after eating raw or undercooked crayfish—Missouri, July 2006–September 2010. MMWR Morb Mortal Wkly Rep 59: 1573–1576.
-
2.
Lane MA, Marcos LA, Onen NF, Demertzis LM, Hayes EV, Davila SZ, Nurutdinova DR, Bailey TC, Weil GJ, 2012. Paragonimus kellicotti flukes in Missouri, USA. Emerg Infect Dis 18: 1263–1267.
-
3.
Lane MA, Barsanti MC, Santos CA, Yeung M, Lubner SJ, Weil GJ, 2009. Human paragonimiasis in North America following ingestion of raw crayfish. Clin Infect Dis 49: e55–e61.
-
4.
Procop GW, 2009. North American paragonimiasis (caused by Paragonimus kellicotti) in the context of global paragonimiasis. Clin Microbiol Rev 22: 415–446.
-
5.
Fischer PU, Curtis KC, Marcos LA, Weil GJ, 2011. Molecular characterization of the North American lung fluke Paragonimus kellicotti in Missouri and its development in Mongolian gerbils. Am J Trop Med Hyg 84: 1005–1011.
-
6.
Slemenda SB, Maddison SE, Jong EC, Moore DD, 1988. Diagnosis of paragonimiasis by immunoblot. Am J Trop Med Hyg 39: 469–471.
-
7.
Chaffee EF, Bauman PM, Shapilo JJ, 1954. Diagnosis of schistosomiasis by complement-fixation. Am J Trop Med Hyg 3: 905–913.
-
8.
Weil GJ, Ogunrinade AF, Chandrashekar R, Kale OO, 1990. IgG4 subclass antibody serology for onchocerciasis. J Infect Dis 161: 549–554.
-
9.
Maleewong W, Wongkham C, Pariyanonda S, Intapan P, Pipitgool V, Daenseegaew W, Morakote N, 1991. Antigenic components of Paragonimus heterotremus recognized by infected human serum. Parasite Immunol 13: 89–93.
-
10.
Indrawati I, Chaicumpa W, Setasuban P, Ruangkunaporn Y, 1991. Studies on immunodiagnosis of human paragonimiasis and specific antigen of Paragonimus heterotremus. Int J Parasitol 21: 395–401.
-
11.
Maleewong W, Wongkham C, Intapan P, Pariyanonda S, Morakote N, 1992. Excretory-secretory antigenic components of Paragonimus heterotremus recognized by infected human sera. J Clin Microbiol 30: 2077–2079.
-
12.
Nakamura-Uchiyama F, Onah DN, Nawa Y, 2001. Clinical features of paragonimiasis cases recently found in Japan: parasite-specific immunoglobulin M and G antibody classes. Clin Infect Dis 32: e151–e153.
-
13.
Blair D, Wu B, Chang ZS, Gong X, Agatsuma T, Zhang YN, Chen SH, Lin JX, Chen MG, Waikagul J, Guevara AG, Feng Z, Davis GM, 1999. A molecular perspective on the genera Paragonimus Braun, Euparagonimus Chen and Pagumogonimus Chen. J Helminthol 73: 295–299.
-
14.
Blair D, Xu ZB, Agatsuma T, 1999. Paragonimiasis and the genus Paragonimus. Adv Parasitol 42: 113–222.
-
15.
Aka NA, Assoumou A, Adoubrynk D, Domoua K, Kouadio F, Moyou-Somo R, Nakamura-Uchiyama F, Nawa Y, Rondelaud D, Dreyfuss G, 2008. First findings on the seroepidemiology of human paragonimosis at the anti-tuberculosis centre of Divo, Repubuc of Ivory Coast (West Africa). Parasite 15: 157–161.
-
16.
Doanh PN, Dung do T, Thach DT, Horii Y, Shinohara A, Nawa Y, 2011. Human paragonimiasis in Viet Nam: epidemiological survey and identification of the responsible species by DNA sequencing of eggs in patients' sputum. Parasitol Int 60: 534–537.
-
17.
Nkouawa A, Okamoto M, Mabou AK, Edinga E, Yamasaki H, Sako Y, Nakao M, Nakaya K, Blair D, Agatsuma T, Enyong P, Shibahara T, Moyou-Somo R, Ito A, 2009. Paragonimiasis in Cameroon: molecular identification, serodiagnosis and clinical manifestations. Trans R Soc Trop Med Hyg 103: 255–261.
-
18.
Yong TS, Seo JH, Yeo IS, 1993. Serodiagnosis of human paragonimiasis by ELISA-inhibition test using monoclonal antibodies. Korean J Parasitol 31: 141–147.
-
19.
Lee JS, Lee J, Kim SH, Yong TS, 2007. Molecular cloning and characterization of a major egg antigen in Paragonimus westermani and its use in ELISA for the immunodiagnosis of paragonimiasis. Parasitol Res 100: 677–681.
-
20.
Yang SH, Park JO, Lee JH, Jeon BH, Kim WS, Kim SI, Yun KJ, Jeong ET, Lee KW, Kim YM, Lee MH, Park H, 2004. Cloning and characterization of a new cysteine proteinase secreted by Paragonimus westermani adult worms. Am J Trop Med Hyg 71: 87–92.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 444 | 370 | 103 |
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Serological Diagnosis of North American Paragonimiasis by Western Blot Using Paragonimus kellicotti Adult Worm Antigen
Peter U. Fischer
Peter U. Fischer
Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri; Heartland Regional Medical Center, St. Joseph, Missouri; Centers for Disease Control and Prevention, Atlanta, Georgia
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Kurt C. Curtis
Kurt C. Curtis
Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri; Heartland Regional Medical Center, St. Joseph, Missouri; Centers for Disease Control and Prevention, Atlanta, Georgia
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Scott M. Folk
Scott M. Folk
Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri; Heartland Regional Medical Center, St. Joseph, Missouri; Centers for Disease Control and Prevention, Atlanta, Georgia
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Patricia P. Wilkins
Patricia P. Wilkins
Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri; Heartland Regional Medical Center, St. Joseph, Missouri; Centers for Disease Control and Prevention, Atlanta, Georgia
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Luis A. Marcos
Luis A. Marcos
Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri; Heartland Regional Medical Center, St. Joseph, Missouri; Centers for Disease Control and Prevention, Atlanta, Georgia
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Gary J. Weil
Gary J. Weil
Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri; Heartland Regional Medical Center, St. Joseph, Missouri; Centers for Disease Control and Prevention, Atlanta, Georgia
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We studied the value of an IgG Western blot (WB) with Paragonimus kellicotti (Pk) antigen for diagnosis of North American paragonimiasis. The test was evaluated with sera from patients with Pk and Paragonimus westermani infections, with control sera from patients with other helminth infections, and sera from healthy Americans. All 11 proven Pk infection sera and two samples from suspected cases that were negative by P. westermani WB at the Centers for Disease Control and Prevention (CDC) contained antibodies to antigens at 34 kDa and at 21/23 kDa. Seven of 7 P. westermani sera contained antibodies to the 34 kDa antigen, but only 2 recognized the 21/23 kDa doublet. No control samples were reactive with these antigens. Antibody reactivity declined after praziquantel treatment. Thus, the P. kellicotti WB appears to be superior to P. westermani WB for diagnosing Pk infections, and it may be useful for assessing responses to treatment.
Author Notes
Financial support: This study was supported by a grant from the Barnes Jewish Hospital Foundation.
Authors' addresses: Peter U. Fischer, Kurt C. Curtis, Luis A. Marcos, and Gary J. Weil, Infectious Diseases Division, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO, E-mails: Pufische@DOM.wustl.edu, KCurtis@DOM.wustl.edu, LMarcos@DOM.wustl.edu, and GWeil@DOM.wustl.edu. Scott M. Folk, Heartland Regional Medical Center, St. Joseph, MO, E-mail: scott.folk@heartland-health.com. Patricia P. Wilkins, Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, Atlanta, GA, E-mail: pma1@cdc.gov.
- Introduction
- Material And Methods
- Results
- Identification of diagnostic proteins.
- Specificity of the P. kellicotti diagnostic proteins.
- Reactivity to excretory/secretory P. kellicotti antigens.
- Emergence and persistence of antibodies to P. kellicotti antigens.
- Comparison of Western blot results obtained with P. kellicotti and P. westermani adult worm antigens.
- Discussion
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Centers for Disease Control and Prevention, 2010. Human paragonimiasis after eating raw or undercooked crayfish—Missouri, July 2006–September 2010. MMWR Morb Mortal Wkly Rep 59: 1573–1576.
-
2.
Lane MA, Marcos LA, Onen NF, Demertzis LM, Hayes EV, Davila SZ, Nurutdinova DR, Bailey TC, Weil GJ, 2012. Paragonimus kellicotti flukes in Missouri, USA. Emerg Infect Dis 18: 1263–1267.
-
3.
Lane MA, Barsanti MC, Santos CA, Yeung M, Lubner SJ, Weil GJ, 2009. Human paragonimiasis in North America following ingestion of raw crayfish. Clin Infect Dis 49: e55–e61.
-
4.
Procop GW, 2009. North American paragonimiasis (caused by Paragonimus kellicotti) in the context of global paragonimiasis. Clin Microbiol Rev 22: 415–446.
-
5.
Fischer PU, Curtis KC, Marcos LA, Weil GJ, 2011. Molecular characterization of the North American lung fluke Paragonimus kellicotti in Missouri and its development in Mongolian gerbils. Am J Trop Med Hyg 84: 1005–1011.
-
6.
Slemenda SB, Maddison SE, Jong EC, Moore DD, 1988. Diagnosis of paragonimiasis by immunoblot. Am J Trop Med Hyg 39: 469–471.
-
7.
Chaffee EF, Bauman PM, Shapilo JJ, 1954. Diagnosis of schistosomiasis by complement-fixation. Am J Trop Med Hyg 3: 905–913.
-
8.
Weil GJ, Ogunrinade AF, Chandrashekar R, Kale OO, 1990. IgG4 subclass antibody serology for onchocerciasis. J Infect Dis 161: 549–554.
-
9.
Maleewong W, Wongkham C, Pariyanonda S, Intapan P, Pipitgool V, Daenseegaew W, Morakote N, 1991. Antigenic components of Paragonimus heterotremus recognized by infected human serum. Parasite Immunol 13: 89–93.
-
10.
Indrawati I, Chaicumpa W, Setasuban P, Ruangkunaporn Y, 1991. Studies on immunodiagnosis of human paragonimiasis and specific antigen of Paragonimus heterotremus. Int J Parasitol 21: 395–401.
-
11.
Maleewong W, Wongkham C, Intapan P, Pariyanonda S, Morakote N, 1992. Excretory-secretory antigenic components of Paragonimus heterotremus recognized by infected human sera. J Clin Microbiol 30: 2077–2079.
-
12.
Nakamura-Uchiyama F, Onah DN, Nawa Y, 2001. Clinical features of paragonimiasis cases recently found in Japan: parasite-specific immunoglobulin M and G antibody classes. Clin Infect Dis 32: e151–e153.
-
13.
Blair D, Wu B, Chang ZS, Gong X, Agatsuma T, Zhang YN, Chen SH, Lin JX, Chen MG, Waikagul J, Guevara AG, Feng Z, Davis GM, 1999. A molecular perspective on the genera Paragonimus Braun, Euparagonimus Chen and Pagumogonimus Chen. J Helminthol 73: 295–299.
-
14.
Blair D, Xu ZB, Agatsuma T, 1999. Paragonimiasis and the genus Paragonimus. Adv Parasitol 42: 113–222.
-
15.
Aka NA, Assoumou A, Adoubrynk D, Domoua K, Kouadio F, Moyou-Somo R, Nakamura-Uchiyama F, Nawa Y, Rondelaud D, Dreyfuss G, 2008. First findings on the seroepidemiology of human paragonimosis at the anti-tuberculosis centre of Divo, Repubuc of Ivory Coast (West Africa). Parasite 15: 157–161.
-
16.
Doanh PN, Dung do T, Thach DT, Horii Y, Shinohara A, Nawa Y, 2011. Human paragonimiasis in Viet Nam: epidemiological survey and identification of the responsible species by DNA sequencing of eggs in patients' sputum. Parasitol Int 60: 534–537.
-
17.
Nkouawa A, Okamoto M, Mabou AK, Edinga E, Yamasaki H, Sako Y, Nakao M, Nakaya K, Blair D, Agatsuma T, Enyong P, Shibahara T, Moyou-Somo R, Ito A, 2009. Paragonimiasis in Cameroon: molecular identification, serodiagnosis and clinical manifestations. Trans R Soc Trop Med Hyg 103: 255–261.
-
18.
Yong TS, Seo JH, Yeo IS, 1993. Serodiagnosis of human paragonimiasis by ELISA-inhibition test using monoclonal antibodies. Korean J Parasitol 31: 141–147.
-
19.
Lee JS, Lee J, Kim SH, Yong TS, 2007. Molecular cloning and characterization of a major egg antigen in Paragonimus westermani and its use in ELISA for the immunodiagnosis of paragonimiasis. Parasitol Res 100: 677–681.
-
20.
Yang SH, Park JO, Lee JH, Jeon BH, Kim WS, Kim SI, Yun KJ, Jeong ET, Lee KW, Kim YM, Lee MH, Park H, 2004. Cloning and characterization of a new cysteine proteinase secreted by Paragonimus westermani adult worms. Am J Trop Med Hyg 71: 87–92.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 444 | 370 | 103 |
| Full Text Views | 518 | 12 | 1 |
| PDF Downloads | 131 | 11 | 1 |