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Variation in Mitochondrial NADH Dehydrogenase Subunit 5 and NADH Dehydrogenase Subunit 4 Genes in the Chagas Disease Vector Triatoma infestans (Hemiptera: Reduviidae)
Variation in mitochondrial NADH dehydrogenase subunit 5 (ND5) and NADH dehydrogenase subunit 4 (ND4) genes was surveyed in Triatoma infestans from 24 localities of Argentina. The DNA sequence comparisons of 2,183 basepairs of the mitochondrial genome, which include the complete sequence of ND5 (1,712 basepairs) and 401 basepairs of ND4 genes, showed 19 haplotypes determined by 48 variable sites and a nucleotide diversity value of 0.292%. Twenty-six (65%) substitutions were synonymous, and there were 14 (35%) predicted amino acid replacements in ND5. In ND4, 5 (62.5%) substitutions were synonymous and 3 (37.5%) were replacement sites. Samples from six localities studied shared one haplotype and the rest of the localities had different haplotypes. The amplified regions should be useful for population genetic studies.
Author Notes
Financial support: This study was supported by the grants from the Agencia Nacional de Promoción Científica y Tecnológica (FONCyT), the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) of Argentina, and the Secretaría de Ciencia y Tecnología de la Universidad Nacional de Córdoba. Cintia J. Fernández is a Fellow of FONCyT and Beatriz A. García is Career Investigator of CONICET.
Authors' addresses: Cintia J. Fernández, Alicia R. Pérez de Rosas, and Beatriz A. García, Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Pabellón Argentina 2° Piso, Ciudad Universitaria, 5000 Córdoba, Argentina, E-mails: cjudithfernandez@gmail.com, arperez@biomed.fcm.edu.com.ar, and bgarcia@biomed.uncor.edu.