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Clinic–Epidemiologic Study of Human Infection by Granada Virus, a New Phlebovirus within the Sandfly Fever Naples Serocomplex

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  • Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Granada, Spain; Departamento de Microbiología, Facultad de Farmacia, Universidad de Granada, Granada, Spain

Granada virus (GRV), a new phlebovirus within the Naples serocomplex, has been recently described in phlebotomine sandflies from Spain. The presence of anti-GRV immunoglobulin G (IgG) antibodies was investigated by indirect fluorescence assay (IFA) and neutralization test (NT) in 920 serum samples from the Granada population. By IFA, an overall GRV seroprevalence of 15.8% (N = 145) was observed, significantly increasing up to 65 years. NT was positive in 18% of anti-GRV IFA-positive samples. IgG antibodies against Toscana virus (TOSV), a hyperendemic phlebovirus within Granada province, were detected in 40% of anti-GRV–positive cases. Anti-GRV IgM antibodies were detected in 36 (6.6%) of 547 acute-phase serum samples from individuals with febrile illness, exanthema, and/or acute respiratory infection. All positives were anti-TOSV IgM-negative. GRV may infect humans, with most cases being asymptomatic. The codetection of anti-GRV and anti-TOSV IgG antibodies could be attributable to cross-reactivity or exposure to the same transmission vector.

Author Notes

* Address correspondence to Mercedes Pérez-Ruiz, Avda. Fuerzas Armadas, 2, Granada 18014. E-mail: mercedes.perez.ruiz.sspa@juntadeandalucia.es† These authors contributed equally.

Financial support: This study was supported in part by funds from a research project (0328/2009; Junta de Andalucía, Spain).

Authors' addresses: José María Navarro-Marí, Cristina Gómez-Camarasa, Mercedes Pérez-Ruiz, Sara Sanbonmatsu-Gámez, and Irene Pedrosa-Corral, Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Granada, Spain, E-mails: josem.navarro.sspa@juntadeandalucia.es, gomezcamarasa@hotmail.com, mercedes.perez.ruiz.sspa@juntadeandalucia.es, saral.sanbonmatsu.sspa@juntadeandalucia.es, and irene_pedrosa@msn.com. María Jiménez-Valera, Departamento de Microbiología, Facultad de Farmacia, Universidad de Granada, Granada, Spain, E-mail: mjvalera@ugr.es.

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